Rorug06G0388800

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006024
Physical Location & Seq
Forward (+)
53337733 .. 53338216
484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0388800.1

Sequence Viewer

Length: 453 bp
ATGAAGAGAAACTCACCAGGATCTCTTGAAGCAACTTCAGAAGAGAATAGTATCTACGAAAATATGGCTGGTATGCCCGTCCGTTTCAGTTACAAGAATCTACAAACTGCAACGAATAACTTCTCAAAGAAGCTTGGGCAAGGAGGTTTTGGATCAGTTTATGAGGGAGTTCTTCCAGATGGAACCCAACTGGCTGGGCAGAAAGAGTTCCGAGCTGAAGTAACCACCACCATTGGCAGCATCCATCATTTTCACTTGGTCCGTCTCAAAGGATTCTGTGCAGAAGGAAGCCATCGACTTCTTGCTTATGAATACATGGCAAATGGTTCCCTGGATAAATGGATCTTCAAGAAAACAAATGATGAATGCTTATTGGATTGGGAGACAAGATTTAGTATAGCATTGGGTACAGCAAAAGGACTAGCTTATCTCCATGAAGAATGTGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

150

Amino Acids

16.71

Weight (kDa)

6.2

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 41 - 146 3e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 42 - 147 4.1e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 28, 160, 350
AcuI CTGAAG 2 cut(s) 21, 237
AfaI GTAC 1 cut(s) 409
AgsI TTSAA 2 cut(s) 29, 349
AjnI CCWGG 2 cut(s) 16, 330
AluBI AGCT 3 cut(s) 133, 215, 425
AluI AGCT 3 cut(s) 133, 215, 425
Alw26I GTCTC 2 cut(s) 269, 377
AlwI GGATC 3 cut(s) 28, 160, 350
ApeKI GCWGC 1 cut(s) 237
Asp700I GAANNNNTTC 2 cut(s) 119, 206
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 249
BccI CCATC 3 cut(s) 173, 252, 300
BciT130I CCWGG 2 cut(s) 18, 332
BcoDI GTCTC 2 cut(s) 269, 377
BfaI CTAG 1 cut(s) 422
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 18, 332
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 2 cut(s) 184, 328
BmrFI CCNGG 2 cut(s) 18, 332
BmsI GCATC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 330
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 18, 332
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 1 cut(s) 194
BsgI GTGCAG 1 cut(s) 300
BsmAI GTCTC 2 cut(s) 269, 377
BsmBI CGTCTC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 371
Bsp143I GATC 3 cut(s) 20, 152, 342
BspLI GGNNCC 2 cut(s) 184, 328
BspPI GGATC 3 cut(s) 28, 160, 350
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 20, 152, 342
Bst2UI CCWGG 2 cut(s) 18, 332
Bst6I CTCTTC 1 cut(s) 36
BstF5I GGATG 1 cut(s) 240
BstKTI GATC 3 cut(s) 23, 155, 345
BstMAI GTCTC 2 cut(s) 269, 377
BstMBI GATC 3 cut(s) 20, 152, 342
BstNI CCWGG 2 cut(s) 18, 332
BstSCI CCNGG 2 cut(s) 16, 330
BstV1I GCAGC 1 cut(s) 249
BstX2I RGATCY 2 cut(s) 20, 342
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 2 cut(s) 20, 342
BtsCI GGATG 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 1 cut(s) 408
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 2 cut(s) 316, 434
CviJI RGCY 6 cut(s) 68, 133, 194, 215, 291, 425
CviKI_1 RGCY 6 cut(s) 68, 133, 194, 215, 291, 425
CviQI GTAC 1 cut(s) 408
DpnI GATC 3 cut(s) 22, 154, 344
DpnII GATC 3 cut(s) 20, 152, 342
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 21, 237
EcoRII CCWGG 2 cut(s) 16, 330
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 2 cut(s) 319, 437
FaiI YATR 8 cut(s) 65, 74, 162, 309, 317, 398, 435, 451
FatI CATG 2 cut(s) 315, 433
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 1 cut(s) 422
GluI GCNGC 1 cut(s) 238
GsaI CCCAGC 1 cut(s) 198
Hin1II CATG 2 cut(s) 319, 437
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 3 cut(s) 97, 273, 446
HphI GGTGA 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 40, 212
Hpy188III TCNNGA 3 cut(s) 26, 176, 349
HpyAV CCTTC 1 cut(s) 278
HpyCH4V TGCA 2 cut(s) 110, 281
Hsp92II CATG 2 cut(s) 319, 437
Kzo9I GATC 3 cut(s) 20, 152, 342
LpnPI CCDG 8 cut(s) 3, 30, 54, 176, 180, 189, 317, 344
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 422
MaeIII GTNAC 2 cut(s) 89, 220
MalI GATC 3 cut(s) 22, 154, 344
MboI GATC 3 cut(s) 20, 152, 342
MboII GAAGA 5 cut(s) 16, 53, 164, 337, 449
MflI RGATCY 2 cut(s) 20, 342
MnlI CCTC 2 cut(s) 137, 157
MroXI GAANNNNTTC 2 cut(s) 119, 206
MspR9I CCNGG 2 cut(s) 18, 332
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 2 cut(s) 18, 332
NdeII GATC 3 cut(s) 20, 152, 342
NlaIII CATG 2 cut(s) 319, 437
NlaIV GGNNCC 2 cut(s) 184, 328
PctI GAATGC 1 cut(s) 371
PdmI GAANNNNTTC 2 cut(s) 119, 206
PfeI GAWTC 3 cut(s) 97, 273, 446
PkrI GCNGC 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 16, 330
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 2 cut(s) 16, 330
PspN4I GGNNCC 2 cut(s) 184, 328
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 2 cut(s) 20, 342
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 20, 152, 342
Sau96I GGNCC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 18, 332
SetI ASST 4 cut(s) 135, 148, 217, 427
SfaNI GCATC 1 cut(s) 249
SinI GGWCC 1 cut(s) 259
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 2 cut(s) 16, 330
TaqI TCGA 1 cut(s) 295
TfiI GAWTC 3 cut(s) 97, 273, 446
TseI GCWGC 1 cut(s) 237
TspDTI ATGAA 5 cut(s) 17, 324, 378, 438, 450
TspGWI ACGGA 2 cut(s) 71, 251
VpaK11BI GGWCC 1 cut(s) 259
XmnI GAANNNNTTC 2 cut(s) 119, 206
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.