Rorug06G0393200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0021655
Physical Location & Seq
Reverse (-)
53854209 .. 53855207
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0393200.1

Sequence Viewer

Length: 999 bp
ATGCTGACACTAATGCCACCCCCAAGCCCAGAGTCCCCATGGACCCTCTCCCCTCACCAAACCCCATCCCCTTCACTCCTTTACCACTGCATTGCCTCCCTCTATCGCCGCGAAGGCTCCATCTCCTCCATTGCAGTCTCAAAGCAGCAAGGAGTAGTCTTCACTGGCTCAGAGAGTACCCGAGTTCGTGTGTGGAGACAACCAGACCACATTGAATACGGTTGTCTCAATGCGACTTCAGGTGAAGTACGAGCTATTGCAACTCATGGTAATATGCTTTTCACCTCACACAAAGACCACAAAATCCGAATGTGGAACTTCACGGTCACAGATCACAATTTCAAGTCCAAAAAGGTTTCTTCTCTCCCCAAAACAAGCTCTTTCCCTTTATTTTCAAGATTTACAAACACCAAACAGCAACACAAAGAATGCATTTCCTGCTTGGCATATTATAAAACCGATGGTCTGTTGACTCTGTTGTATACTGGCTCCCATGACAGAACAGTCAAAGCATGGCGGCTTATAACCAATCACTGTGTGGACTCATTTGTGGCATATGAAGACAATGTGAATGCAATGGTGGTGAACCAAGATGATGGGTGCGTTTTCACTTGCTCCTCAGATGGTTCTGTCAAACTCTGGAGAAGGGTTTTCAGAGGAAACTCTCACACTCTCACCATGACTCTCAAATCCCAATTCCCAAATTCCAACTACCCTGTAAAAGCCAGAGCCTTAAACACATCATCCAACTCTTGCTTCCTCTACTCTGGTTCTGCAGACGGAACCATTAGTTTCTGGGAGAAGGAGAAACTGACCCACAGATTTAACCATGGTGGGTTTTTACAGGGCCACCATTTTTCAGTTCTGTGTTTAGTGGCCATCGAGAAGTTGATTTTCAGTGGATCAGAGGATACTACAATTAGGGTTTGGAGGAGGGAAGAAGGGGGTTGTTTTCATGAATGTTTGGCTGTGTTGGAGGGGCATATACATAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

332

Amino Acids

37.52

Weight (kDa)

9.0

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 34 - 132 3.3e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 37 - 226 7e-18 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 50 - 215 4.8e-09 TEP-1 second beta-propeller
WDR55 PF24796 136 - 309 1.1e-11 WDR55
Beta-prop_EML PF23409 138 - 312 7.5e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 139 - 215 1.3e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 139 - 319 1.6e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 141 - 269 6.1e-10 Prp19 WD40 domain
Beta-prop_ACSF4 PF13570 157 - 310 8.3e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR3_2nd PF25172 158 - 269 4.5e-10 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 158 - 318 2.5e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 158 - 240 1.7e-06 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 159 - 311 1.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 242 - 320 6e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 242 - 316 4.3e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 246 - 320 1.3e-08 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 252 - 313 7.6e-06 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021655)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002589.1_g000027 Rmu_sc0003238.1_g000022 Rmu_sc0008559.1_g000009
rosa_rugosa Rorug06G0393200
rosa_samantha Rh4CG057500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 453, 524
AasI GACNNNNNNGTC 1 cut(s) 503
AccI GTMKAC 1 cut(s) 482
AccII CGCG 1 cut(s) 111
AciI CCGC 2 cut(s) 109, 517
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 2 cut(s) 178, 249
AgsI TTSAA 3 cut(s) 215, 343, 396
AjuI GAANNNNNNNTTGG 2 cut(s) 740, 772
AluBI AGCT 2 cut(s) 254, 378
AluI AGCT 2 cut(s) 254, 378
Alw26I GTCTC 3 cut(s) 142, 190, 230
AlwI GGATC 1 cut(s) 910
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 2 cut(s) 847, 876
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 703
AspS9I GGNCC 2 cut(s) 42, 847
AsuHPI GGTGA 5 cut(s) 47, 254, 274, 595, 667
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 42
BalI TGGCCA 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 151, 567
BbvI GCAGC 1 cut(s) 157
BccI CCATC 6 cut(s) 73, 128, 455, 590, 617, 887
BciVI GTATCC 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 142, 190, 230
BfmI CTRYAG 1 cut(s) 774
BfuI GTATCC 1 cut(s) 904
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 3 cut(s) 109, 146, 518
BlsI GCNGC 3 cut(s) 110, 147, 519
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 42, 847
BmiI GGNNCC 4 cut(s) 44, 118, 490, 784
BpiI GAAGAC 2 cut(s) 151, 567
BpmI CTGGAG 1 cut(s) 661
BsaJI CCNNGG 2 cut(s) 38, 829
Bse1I ACTGG 2 cut(s) 169, 490
Bse3DI GCAATG 3 cut(s) 90, 129, 582
BseDI CCNNGG 2 cut(s) 38, 829
BseGI GGATG 2 cut(s) 65, 743
BseMI GCAATG 3 cut(s) 90, 129, 582
BseMII CTCAG 2 cut(s) 183, 633
BseNI ACTGG 2 cut(s) 169, 490
BseRI GAGGAG 3 cut(s) 115, 607, 946
BseXI GCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 849, 878
BsiHKCI CYCGRG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 3 cut(s) 142, 190, 230
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 2 cut(s) 434, 577
BsnI GGCC 2 cut(s) 849, 878
BsoBI CYCGRG 1 cut(s) 180
Bsp143I GATC 2 cut(s) 331, 902
Bsp19I CCATGG 2 cut(s) 38, 829
BspACI CCGC 2 cut(s) 109, 517
BspANI GGCC 2 cut(s) 849, 878
BspCNI CTCAG 2 cut(s) 182, 632
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 4 cut(s) 44, 118, 490, 784
BspMAI CTGCAG 1 cut(s) 778
BspPI GGATC 1 cut(s) 910
BsrDI GCAATG 3 cut(s) 90, 129, 582
BsrI ACTGG 2 cut(s) 169, 490
BssECI CCNNGG 2 cut(s) 38, 829
BssMI GATC 2 cut(s) 331, 902
BssNAI GTATAC 1 cut(s) 483
BssT1I CCWWGG 2 cut(s) 38, 829
Bst1107I GTATAC 1 cut(s) 483
Bst4CI ACNGT 4 cut(s) 221, 325, 505, 536
BstAPI GCANNNNNTGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 169, 619
BstDSI CCRYGG 2 cut(s) 38, 829
BstF5I GGATG 2 cut(s) 65, 743
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 2 cut(s) 334, 905
BstMAI GTCTC 3 cut(s) 142, 190, 230
BstMBI GATC 2 cut(s) 331, 902
BstMWI GCNNNNNNNGC 2 cut(s) 114, 438
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 2 cut(s) 151, 567
BstXI CCANNNNNNTGG 1 cut(s) 596
BstZ17I GTATAC 1 cut(s) 483
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 2 cut(s) 849, 878
BtgI CCRYGG 2 cut(s) 38, 829
BtsCI GGATG 2 cut(s) 65, 743
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 4 cut(s) 85, 162, 532, 904
CciI TCATGA 1 cut(s) 955
Cfr13I GGNCC 2 cut(s) 42, 847
Csp6I GTAC 2 cut(s) 177, 248
CviAII CATG 7 cut(s) 39, 266, 494, 513, 679, 830, 956
CviQI GTAC 2 cut(s) 177, 248
DdeI CTNAG 2 cut(s) 169, 619
DpnI GATC 2 cut(s) 333, 904
DpnII GATC 2 cut(s) 331, 902
DraIII CACNNNGTG 1 cut(s) 538
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 876
Eco130I CCWWGG 2 cut(s) 38, 829
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 180
EcoT14I CCWWGG 2 cut(s) 38, 829
EcoT22I ATGCAT 1 cut(s) 434
ErhI CCWWGG 2 cut(s) 38, 829
FaeI CATG 7 cut(s) 42, 269, 497, 516, 682, 833, 959
FaqI GGGAC 1 cut(s) 19
FatI CATG 7 cut(s) 38, 265, 493, 512, 678, 829, 955
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 3 cut(s) 109, 146, 518
FokI GGATG 2 cut(s) 52, 730
Fsp4HI GCNGC 3 cut(s) 109, 146, 518
GluI GCNGC 3 cut(s) 109, 146, 518
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 2 cut(s) 849, 878
Hin1II CATG 7 cut(s) 42, 269, 497, 516, 682, 833, 959
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HinfI GANTC 4 cut(s) 32, 472, 542, 682
HphI GGTGA 5 cut(s) 47, 254, 274, 595, 667
Hpy166II GTNNAC 4 cut(s) 471, 483, 541, 586
Hpy188I TCNGA 5 cut(s) 172, 308, 622, 656, 907
Hpy188III TCNNGA 4 cut(s) 396, 640, 883, 956
Hpy8I GTNNAC 4 cut(s) 471, 483, 541, 586
HpyAV CCTTC 5 cut(s) 81, 107, 639, 796, 935
HpyCH4III ACNGT 4 cut(s) 221, 325, 505, 536
HpyCH4V TGCA 6 cut(s) 90, 134, 260, 432, 575, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 438
HpyF3I CTNAG 2 cut(s) 169, 619
Hsp92II CATG 7 cut(s) 42, 269, 497, 516, 682, 833, 959
Kzo9I GATC 2 cut(s) 331, 902
LmnI GCTCC 3 cut(s) 122, 494, 620
Lsp1109I GCAGC 1 cut(s) 157
MaeIII GTNAC 1 cut(s) 325
MalI GATC 2 cut(s) 333, 904
MboI GATC 2 cut(s) 331, 902
MboII GAAGA 4 cut(s) 151, 351, 572, 950
MlsI TGGCCA 1 cut(s) 878
MluCI AATT 4 cut(s) 337, 695, 703, 918
MluNI TGGCCA 1 cut(s) 878
MlyI GAGTC 4 cut(s) 41, 466, 536, 676
MmeI TCCRAC 3 cut(s) 732, 771, 954
Mox20I TGGCCA 1 cut(s) 878
Mph1103I ATGCAT 1 cut(s) 434
MscI TGGCCA 1 cut(s) 878
MseI TTAA 2 cut(s) 734, 825
Msp20I TGGCCA 1 cut(s) 878
Mva1269I GAATGC 2 cut(s) 434, 577
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 114, 438
NcoI CCATGG 2 cut(s) 38, 829
NdeI CATATG 1 cut(s) 556
NdeII GATC 2 cut(s) 331, 902
NlaIII CATG 7 cut(s) 42, 269, 497, 516, 682, 833, 959
NlaIV GGNNCC 4 cut(s) 44, 118, 490, 784
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 434
PagI TCATGA 1 cut(s) 955
PctI GAATGC 2 cut(s) 434, 577
PkrI GCNGC 3 cut(s) 110, 147, 519
PleI GAGTC 4 cut(s) 40, 466, 536, 676
PpsI GAGTC 4 cut(s) 40, 466, 536, 676
PsiI TTATAA 2 cut(s) 453, 524
PspN4I GGNNCC 4 cut(s) 44, 118, 490, 784
PspPI GGNCC 2 cut(s) 42, 847
PstI CTGCAG 1 cut(s) 778
RsaI GTAC 2 cut(s) 178, 249
RsaNI GTAC 2 cut(s) 177, 248
SaqAI TTAA 2 cut(s) 734, 825
SatI GCNGC 3 cut(s) 109, 146, 518
Sau3AI GATC 2 cut(s) 331, 902
Sau96I GGNCC 2 cut(s) 42, 847
SchI GAGTC 4 cut(s) 41, 466, 536, 676
SetI ASST 5 cut(s) 244, 256, 287, 357, 380
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 1 cut(s) 42
Sse9I AATT 4 cut(s) 337, 695, 703, 918
SsiI CCGC 2 cut(s) 109, 517
StyI CCWWGG 2 cut(s) 38, 829
TaaI ACNGT 4 cut(s) 221, 325, 505, 536
TaqI TCGA 1 cut(s) 882
TasI AATT 4 cut(s) 337, 695, 703, 918
TauI GCSGC 2 cut(s) 111, 520
Tru1I TTAA 2 cut(s) 734, 825
Tru9I TTAA 2 cut(s) 734, 825
TscAI CASTG 4 cut(s) 92, 169, 539, 904
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 573, 944, 972
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 4 cut(s) 92, 169, 539, 904
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 703
XcmI CCANNNNNNNNNTGG 2 cut(s) 36, 535
XmiI GTMKAC 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.