Rorug06G0492400

BONZAI 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007922
Physical Location & Seq
Reverse (-)
61018891 .. 61024299
5409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0492400.1

Sequence Viewer

Length: 1062 bp
ATGCTCGGTGTTGGACGAGAGGTGATCGAGAATTTCAAGCTTAGAGGCACATTTTCAAGAGATTTGAGCGGTTTCGTGAAGAATCCATTTGCATTCTGCCTAGTTCTGGTGCGCCAGCAGAGCAAGATTTCCGAAAGGACGGAAGTTTCTTCTGCGAGTTTAATCTTAGTAAATACAGGTGTACAAAAGGAGATGGAAAGAGACTATGTGAGGCACGCCAATATTAACGACACCAAGAATTCTAGAGACAGAACCGGAGGGGATAGCTCACTGCTGTTGAGATCGAGTCCTGATAAAACGACGACGACGTCGGAGTTCGTTTTGCAGTGGGGGAATCGCAAGCGCCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGTGGTGTCCCGGTTACCAGAACCACGGTTCGGGTAGACCGCCGGGTCATTCGACCGGACAAGGACTCGTCCAATCAGCCCTCTACCACCAATAATAGTAACGGGTATTTGAACCTCCGTCAACGACCGTTATCGCCGCAACAACCACCACCTCAGCGTGTTCTCAGAAACTCGGAGACTTCAAGTGCAGGCATGAGAGGCCAGAGCAACGGAGCTATGAGGGGAATTATTTCGCCTGACAGGGGCGGCGTTGCGCACGATAAGAAAGGTACAACTAACAACCACAACCACAATCATCACCATCACCATAATGACAATCATCACAACCACAAGTCCGCTGCGTCATCGGAGACGGCGCACGATAGCAAGAAGGGCGGCTCCTCATCGGGGAGCGGCGACGCGGCAGCAGCACCGCCGATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAGAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGAGACCCAAAAAGAGAGCAAAGATCATCCAACGCACCCTCAATCTCGTAAGCCCGGGTGCATGGCTGTGTGATCTGACGCTGGAACGGTACGAGGTCAGAGAGAAGAAGATCTCCAAGAAGAGACCGAGAGGATTGAAGGCAATGGGCACCATGGTGGAGTCGGACTCGGAATAG

Protein Analysis

353

Amino Acids

39.33

Weight (kDa)

10.24

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 277 - 326 7.5e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 307, 425
AatII GACGTC 1 cut(s) 311
Acc16I TGCGCA 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 1034
AccBSI CCGCTC 2 cut(s) 69, 774
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 782
AclWI GGATC 3 cut(s) 355, 867, 880
AcoI YGGCCR 2 cut(s) 806, 861
AcsI RAATTY 2 cut(s) 31, 238
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 3 cut(s) 183, 652, 977
AfiI CCNNNNNNNGG 5 cut(s) 106, 370, 411, 623, 768
AgsI TTSAA 5 cut(s) 37, 57, 493, 564, 1024
AjnI CCWGG 1 cut(s) 363
AleI CACNNNNGTG 1 cut(s) 1040
AluBI AGCT 4 cut(s) 40, 267, 596, 880
AluI AGCT 4 cut(s) 40, 267, 596, 880
Alw26I GTCTC 6 cut(s) 195, 240, 551, 725, 883, 1003
AlwI GGATC 3 cut(s) 355, 867, 880
Ama87I CYCGRG 1 cut(s) 940
AoxI GGCC 3 cut(s) 580, 806, 861
ApeKI GCWGC 3 cut(s) 719, 785, 788
ApoI RAATTY 2 cut(s) 31, 238
Asp700I GAANNNNTTC 1 cut(s) 610
AspLEI GCGC 4 cut(s) 114, 345, 637, 739
AsuC2I CCSGG 4 cut(s) 392, 425, 941, 942
AsuHPI GGTGA 3 cut(s) 34, 671, 677
AvaI CYCGRG 1 cut(s) 940
BaeGI GKGCMC 1 cut(s) 1037
BalI TGGCCA 1 cut(s) 863
BamHI GGATCC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 1034
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 3 cut(s) 706, 797, 800
BccI CCATC 3 cut(s) 187, 690, 793
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 1 cut(s) 365
BcnI CCSGG 4 cut(s) 392, 425, 941, 942
BcoDI GTCTC 6 cut(s) 195, 240, 551, 725, 883, 1003
BfaI CTAG 2 cut(s) 101, 243
BfoI RGCGCY 1 cut(s) 346
BglII AGATCT 1 cut(s) 996
BisI GCNGC 9 cut(s) 518, 628, 720, 757, 775, 783, 786, 789, 809
BlsI GCNGC 9 cut(s) 519, 629, 721, 758, 776, 784, 787, 790, 810
Bme1390I CCNGG 5 cut(s) 365, 392, 425, 941, 942
BmeT110I CYCGRG 1 cut(s) 940
BmiI GGNNCC 3 cut(s) 760, 874, 1036
BmrFI CCNGG 5 cut(s) 365, 392, 425, 941, 942
BplI GAGNNNNNCTC 1 cut(s) 1037
BpmI CTGGAG 1 cut(s) 364
Bpu10I CCTNAGC 1 cut(s) 534
BpuMI CCSGG 4 cut(s) 392, 425, 941, 942
BsaHI GRCGYC 1 cut(s) 308
BsaI GGTCTC 2 cut(s) 883, 1003
BsaJI CCNNGG 3 cut(s) 405, 940, 1038
BsaWI WCCGGW 3 cut(s) 254, 348, 436
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 370, 411, 623, 768
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 3 cut(s) 405, 940, 1038
BseGI GGATG 1 cut(s) 912
BseLI CCNNNNNNNGG 5 cut(s) 106, 370, 411, 623, 768
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 3 cut(s) 548, 559, 899
BseNI ACTGG 1 cut(s) 381
BseRI GAGGAG 1 cut(s) 751
BseSI GKGCMC 1 cut(s) 1037
BseXI GCAGC 3 cut(s) 706, 797, 800
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 782
Bsh1285I CGRYCG 2 cut(s) 437, 509
BshFI GGCC 3 cut(s) 582, 808, 863
BshNI GGYRCC 1 cut(s) 1034
BsiEI CGRYCG 2 cut(s) 437, 509
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 6 cut(s) 255, 349, 392, 424, 437, 941
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 5 cut(s) 106, 370, 411, 623, 768
BsmAI GTCTC 6 cut(s) 195, 240, 551, 725, 883, 1003
BsmBI CGTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 3 cut(s) 582, 808, 863
Bso31I GGTCTC 2 cut(s) 883, 1003
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 1037
Bsp1407I TGTACA 1 cut(s) 181
Bsp143I GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
Bsp19I CCATGG 1 cut(s) 1038
BspANI GGCC 3 cut(s) 582, 808, 863
BspCNI CTCAG 3 cut(s) 547, 558, 898
BspFNI CGCG 1 cut(s) 782
BspLI GGNNCC 3 cut(s) 760, 874, 1036
BspPI GGATC 3 cut(s) 355, 867, 880
BspT107I GGYRCC 1 cut(s) 1034
BspTNI GGTCTC 2 cut(s) 883, 1003
BsrBI CCGCTC 2 cut(s) 69, 774
BsrDI GCAATG 1 cut(s) 1035
BsrGI TGTACA 1 cut(s) 181
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 3 cut(s) 405, 940, 1038
BssMI GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 1038
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 3 cut(s) 409, 510, 975
Bst6I CTCTTC 2 cut(s) 843, 1001
BstACI GRCGYC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 181
BstC8I GCNNGC 4 cut(s) 116, 216, 341, 571
BstDEI CTNAG 5 cut(s) 41, 166, 534, 545, 885
BstDSI CCRYGG 2 cut(s) 405, 1038
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 1 cut(s) 912
BstFNI CGCG 1 cut(s) 782
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 4 cut(s) 114, 345, 637, 739
BstKTI GATC 7 cut(s) 27, 284, 363, 875, 912, 961, 999
BstMAI GTCTC 6 cut(s) 195, 240, 551, 725, 883, 1003
BstMBI GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
BstMCI CGRYCG 2 cut(s) 437, 509
BstMWI GCNNNNNNNGC 5 cut(s) 120, 351, 579, 753, 788
BstNI CCWGG 1 cut(s) 365
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 5 cut(s) 363, 390, 423, 939, 940
BstSLI GKGCMC 1 cut(s) 1037
BstUI CGCG 1 cut(s) 782
BstV1I GCAGC 3 cut(s) 706, 797, 800
BstX2I RGATCY 3 cut(s) 360, 872, 996
BstYI RGATCY 3 cut(s) 360, 872, 996
BsuRI GGCC 3 cut(s) 582, 808, 863
BtgI CCRYGG 2 cut(s) 405, 1038
BtsCI GGATG 1 cut(s) 912
BtsI GCAGTG 2 cut(s) 269, 332
BtsIMutI CAGTG 3 cut(s) 269, 332, 388
Cac8I GCNNGC 4 cut(s) 116, 216, 341, 571
CfoI GCGC 4 cut(s) 114, 345, 637, 739
Cfr9I CCCGGG 1 cut(s) 940
CseI GACGC 3 cut(s) 711, 788, 973
Csp6I GTAC 3 cut(s) 182, 651, 976
CviAII CATG 5 cut(s) 355, 574, 859, 948, 1039
CviQI GTAC 3 cut(s) 182, 651, 976
DdeI CTNAG 5 cut(s) 41, 166, 534, 545, 885
DpnI GATC 7 cut(s) 26, 283, 362, 874, 911, 960, 998
DpnII GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
DrdI GACNNNNNNGTC 2 cut(s) 307, 425
DseDI GACNNNNNNGTC 2 cut(s) 307, 425
EaeI YGGCCR 2 cut(s) 806, 861
Eam1104I CTCTTC 2 cut(s) 843, 1001
EarI CTCTTC 2 cut(s) 843, 1001
Eco130I CCWWGG 1 cut(s) 1038
Eco31I GGTCTC 2 cut(s) 883, 1003
Eco88I CYCGRG 1 cut(s) 940
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 1038
Esp3I CGTCTC 1 cut(s) 725
FaeI CATG 5 cut(s) 358, 577, 862, 951, 1042
FaiI YATR 8 cut(s) 207, 356, 575, 599, 690, 860, 949, 1040
FaqI GGGAC 1 cut(s) 374
FatI CATG 5 cut(s) 354, 573, 858, 947, 1038
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 9 cut(s) 518, 628, 720, 757, 775, 783, 786, 789, 809
FokI GGATG 1 cut(s) 899
Fsp4HI GCNGC 9 cut(s) 518, 628, 720, 757, 775, 783, 786, 789, 809
FspBI CTAG 2 cut(s) 101, 243
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 4 cut(s) 113, 344, 636, 738
GluI GCNGC 9 cut(s) 518, 628, 720, 757, 775, 783, 786, 789, 809
GsuI CTGGAG 1 cut(s) 364
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 3 cut(s) 582, 808, 863
HapII CCGG 6 cut(s) 255, 349, 392, 424, 437, 941
HgaI GACGC 3 cut(s) 711, 788, 973
HhaI GCGC 4 cut(s) 114, 345, 637, 739
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 5 cut(s) 358, 577, 862, 951, 1042
Hin6I GCGC 4 cut(s) 112, 343, 635, 737
HinP1I GCGC 4 cut(s) 112, 343, 635, 737
HincII GTYRAC 1 cut(s) 503
HindII GTYRAC 1 cut(s) 503
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 7 cut(s) 82, 286, 334, 377, 446, 1046, 1052
HpaII CCGG 6 cut(s) 255, 349, 392, 424, 437, 941
HphI GGTGA 3 cut(s) 34, 671, 677
Hpy166II GTNNAC 3 cut(s) 182, 418, 503
Hpy188III TCNNGA 6 cut(s) 28, 57, 76, 243, 290, 820
Hpy8I GTNNAC 3 cut(s) 182, 418, 503
Hpy99I CGWCG 5 cut(s) 304, 307, 310, 313, 782
HpyAV CCTTC 3 cut(s) 745, 838, 1018
HpyCH4III ACNGT 3 cut(s) 409, 510, 975
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 5 cut(s) 92, 325, 354, 569, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 351, 579, 753, 788
HpyF3I CTNAG 5 cut(s) 41, 166, 534, 545, 885
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 5 cut(s) 358, 577, 862, 951, 1042
HspAI GCGC 4 cut(s) 112, 343, 635, 737
Kzo9I GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
LmnI GCTCC 4 cut(s) 593, 764, 771, 885
Lsp1109I GCAGC 3 cut(s) 706, 797, 800
MaeI CTAG 2 cut(s) 101, 243
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 2 cut(s) 394, 479
MalI GATC 7 cut(s) 26, 283, 362, 874, 911, 960, 998
MbiI CCGCTC 2 cut(s) 69, 774
MboI GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
MboII GAAGA 7 cut(s) 91, 141, 370, 860, 1003, 1006, 1018
MflI RGATCY 3 cut(s) 360, 872, 996
MhlI GDGCHC 1 cut(s) 1037
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 3 cut(s) 31, 238, 606
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 5 cut(s) 295, 371, 440, 1046, 1055
MmeI TCCRAC 3 cut(s) 291, 940, 1029
Mox20I TGGCCA 1 cut(s) 863
MroXI GAANNNNTTC 1 cut(s) 610
MscI TGGCCA 1 cut(s) 863
MseI TTAA 3 cut(s) 161, 225, 867
MslI CAYNNNNRTG 3 cut(s) 690, 952, 1040
Msp20I TGGCCA 1 cut(s) 863
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 6 cut(s) 255, 349, 392, 424, 437, 941
MspR9I CCNGG 5 cut(s) 365, 392, 425, 941, 942
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 5 cut(s) 120, 351, 579, 753, 788
NciI CCSGG 4 cut(s) 392, 425, 941, 942
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
NlaIII CATG 5 cut(s) 358, 577, 862, 951, 1042
NlaIV GGNNCC 3 cut(s) 760, 874, 1036
NsbI TGCGCA 1 cut(s) 636
OliI CACNNNNGTG 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PctI GAATGC 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 2 cut(s) 82, 334
PflFI GACNNNGTC 1 cut(s) 307
PkrI GCNGC 9 cut(s) 519, 629, 721, 758, 776, 784, 787, 790, 810
PleI GAGTC 5 cut(s) 294, 371, 440, 1046, 1054
PpsI GAGTC 5 cut(s) 294, 371, 440, 1046, 1054
Psp6I CCWGG 1 cut(s) 363
PspEI GGTNACC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 3 cut(s) 760, 874, 1036
PsuI RGATCY 3 cut(s) 360, 872, 996
PsyI GACNNNGTC 1 cut(s) 307
RsaI GTAC 3 cut(s) 183, 652, 977
RsaNI GTAC 3 cut(s) 182, 651, 976
RseI CAYNNNNRTG 3 cut(s) 690, 952, 1040
SaqAI TTAA 3 cut(s) 161, 225, 867
SatI GCNGC 9 cut(s) 518, 628, 720, 757, 775, 783, 786, 789, 809
Sau3AI GATC 7 cut(s) 24, 281, 360, 872, 909, 958, 996
SchI GAGTC 5 cut(s) 295, 371, 440, 1046, 1055
ScrFI CCNGG 5 cut(s) 365, 392, 425, 941, 942
SduI GDGCHC 1 cut(s) 1037
SmaI CCCGGG 1 cut(s) 942
SmiMI CAYNNNNRTG 3 cut(s) 690, 952, 1040
Sse9I AATT 3 cut(s) 31, 238, 606
SspI AATATT 1 cut(s) 223
SspMI CTAG 2 cut(s) 101, 243
StyD4I CCNGG 5 cut(s) 363, 390, 423, 939, 940
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 3 cut(s) 409, 510, 975
TaiI ACGT 1 cut(s) 311
TaqI TCGA 3 cut(s) 27, 284, 433
TaqII GACCGA 1 cut(s) 1027
TasI AATT 3 cut(s) 31, 238, 606
TatI WGTACW 1 cut(s) 181
TauI GCSGC 6 cut(s) 520, 630, 759, 777, 785, 811
TfiI GAWTC 2 cut(s) 82, 334
Tru1I TTAA 3 cut(s) 161, 225, 867
Tru9I TTAA 3 cut(s) 161, 225, 867
TscAI CASTG 3 cut(s) 276, 332, 388
TseI GCWGC 3 cut(s) 719, 785, 788
TspDTI ATGAA 2 cut(s) 371, 847
TspGWI ACGGA 3 cut(s) 155, 488, 606
TspMI CCCGGG 1 cut(s) 940
TspRI CASTG 3 cut(s) 276, 332, 388
Tth111I GACNNNGTC 1 cut(s) 307
XapI RAATTY 2 cut(s) 31, 238
XbaI TCTAGA 1 cut(s) 242
XmaI CCCGGG 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 2 cut(s) 101, 243
ZraI GACGTC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.