Rh1AG421300

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0020805
Physical Location & Seq
Forward (+)
65120127 .. 65132730
12604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG421300.1

Sequence Viewer

Length: 1200 bp
ATGACCTCCGATGACCTCTCCTCCGTGTCGACCACCGCTGCTACAAGAACCCCAAGGCCTGGCCCCCTCGCGTTGTTCCTCTGCCACATGTTTCTCGCTCCTCTCATGTTGACTTTCTCTGTATTATCTCTAACCTTTCAAGTTTCAATCTCTTCCTTCTCTCTTCTTTCTCTCAGGTATGCCATCTCTTTTCTTCTCTCTGCCTGCTTAGCACCATTGGCATCACCAACTACAATCGGAGGTCCGAGTTCGACTCGGACTCGGACTCTCGCAACATGTCGGACTCGGCGGCGTTCTTCGAGTTGCTGTGTCAGCGCCAGGAGGGTAAAATATGGGGGTGTTCGTCTTCTTCGAGCTCAAATTGGCGATCGCCCTAACGCCCAAACACCGCCAAGCTTGGCGTCAAGAAATTCGATAATGGCAATGGTGGCCGCTTCTCACAACCTCATTTACCCAGCCTACAACTTCTCTGTCATCGAGTATTTTCATCGCTTTCCCGCTAGAACATCACATCCTTCAGTTGGTGATCTGAATCATCTGATTTCGGCTACCATGAGTGGTGTAACCTGCTGCCTTCGTTTCCCTGGACAACTCAACTCTGATCTCCGCAAGCTTGCTGTTAATCTGATCCCATTCCCCCGATTGCATTTCTTCATGGTTGGGTTTGCTCCACTTACATCTCGTGGATCGCAGCAATACAGGGCACTCACTGTTCCAGAGCTCACTCAACAGATGTGGGATGCCAAGAACATGATGTGTGCTGCTGATCCTCGTCACGGGCGGTATTTGACTGCTTCAGCAATGTTCCGTGGTAAGATGAGCACCAAGGAAGTTGATGAACAGATGATCAATGTCCAAAACAAGAATTCATCCTACTTTGTGGAGTGGATCCCCAACAATGTCAAGTCCACTGTTTGTGACATCCCACCAACCGGTCTGAAGATGGCTTCGACATTCATTGGCAACTCCACTTCCATTCAAGAAATGTTCCGGAGGGTGAGTGAGCAGTTCACTGCTATGTTCCGCAGAAAGGCTTTCTTGCATTGGTACACAGGAGAGGGAATGGATGAGATGGAGTTTACTGAGGCAGAGAGCAACATGAATGACCTTGTTTCAGAGTACCAGCAGTACCAGGATGCCACTGCAGATGAGGAAGGGTACGATTATGAAGATGAGGAGGACGTTCAGGAGGAGGCTTGA

Protein Analysis

399

Amino Acids

44.63

Weight (kDa)

8.05

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 213 - 334 1.4e-40 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020805)

Species Orthologous Gene IDs
rosa_samantha Rh1AG421300 Rh1CG057500 Rh5BG407100 Rh7CG298500 Rh7CG492400 Rh7DG460500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 575
AccB7I CCANNNNNTGG 1 cut(s) 59
AccI GTMKAC 1 cut(s) 29
AccII CGCG 1 cut(s) 71
AccIII TCCGGA 1 cut(s) 990
AciI CCGC 8 cut(s) 36, 289, 389, 432, 498, 607, 781, 1024
AclWI GGATC 5 cut(s) 622, 694, 761, 883, 896
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 2 cut(s) 409, 865
AcuI CTGAAG 3 cut(s) 501, 780, 959
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 4 cut(s) 1049, 1121, 1130, 1160
AfiI CCNNNNNNNGG 5 cut(s) 59, 521, 776, 932, 1030
AflIII ACRYGT 2 cut(s) 87, 275
AgeI ACCGGT 1 cut(s) 932
AgsI TTSAA 3 cut(s) 140, 147, 980
AjnI CCWGG 4 cut(s) 58, 317, 583, 1131
AloI GAACNNNNNNTCC 2 cut(s) 496, 528
AluBI AGCT 4 cut(s) 356, 396, 613, 721
AluI AGCT 4 cut(s) 356, 396, 613, 721
Alw21I GWGCWC 3 cut(s) 358, 723, 824
AlwI GGATC 5 cut(s) 622, 694, 761, 883, 896
Aor13HI TCCGGA 1 cut(s) 990
AoxI GGCC 3 cut(s) 56, 61, 429
ApeKI GCWGC 4 cut(s) 38, 570, 691, 761
ApoI RAATTY 2 cut(s) 409, 865
AsiGI ACCGGT 1 cut(s) 932
AsiSI GCGATCGC 1 cut(s) 370
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 62, 242
AsuHPI GGTGA 3 cut(s) 216, 536, 1009
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 706
BamHI GGATCC 1 cut(s) 888
BanII GRGCYC 2 cut(s) 358, 723
BauI CACGAG 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 338
Bbv12I GWGCWC 3 cut(s) 358, 723, 824
BbvI GCAGC 4 cut(s) 25, 557, 703, 748
BccI CCATC 3 cut(s) 191, 937, 1066
BciT130I CCWGG 4 cut(s) 60, 319, 585, 1133
BclI TGATCA 1 cut(s) 846
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 1143
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 6 cut(s) 39, 290, 432, 571, 692, 762
BlpI GCTNAGC 1 cut(s) 208
BlsI GCNGC 6 cut(s) 40, 291, 433, 572, 693, 763
Bme1390I CCNGG 4 cut(s) 60, 319, 585, 1133
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 62, 242
BmiI GGNNCC 2 cut(s) 64, 890
BmrFI CCNGG 4 cut(s) 60, 319, 585, 1133
BmsI GCATC 3 cut(s) 230, 730, 1126
BpiI GAAGAC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu1102I GCTNAGC 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 4 cut(s) 53, 583, 808, 825
BsaWI WCCGGW 2 cut(s) 932, 990
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 985, 1015
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 521, 776, 932, 1030
Bse118I RCCGGY 1 cut(s) 932
Bse3DI GCAATG 2 cut(s) 429, 807
Bse8I GATNNNNATC 1 cut(s) 531
BseAI TCCGGA 1 cut(s) 990
BseBI CCWGG 4 cut(s) 60, 319, 585, 1133
BseDI CCNNGG 4 cut(s) 53, 583, 808, 825
BseGI GGATG 6 cut(s) 511, 745, 869, 921, 1072, 1141
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 5 cut(s) 59, 521, 776, 932, 1030
BseMI GCAATG 2 cut(s) 429, 807
BseMII CTCAG 2 cut(s) 187, 1074
BseRI GAGGAG 3 cut(s) 10, 90, 1190
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 4 cut(s) 25, 557, 703, 748
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 58, 63, 431
BshTI ACCGGT 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 3 cut(s) 358, 723, 824
BsiSI CCGG 2 cut(s) 933, 991
BslI CCNNNNNNNGG 5 cut(s) 59, 521, 776, 932, 1030
BsnI GGCC 3 cut(s) 58, 63, 431
Bsp1286I GDGCHC 4 cut(s) 358, 706, 723, 824
Bsp13I TCCGGA 1 cut(s) 990
Bsp143I GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
Bsp1720I GCTNAGC 1 cut(s) 208
BspACI CCGC 8 cut(s) 36, 289, 389, 432, 498, 607, 781, 1024
BspANI GGCC 3 cut(s) 58, 63, 431
BspCNI CTCAG 2 cut(s) 186, 1075
BspEI TCCGGA 1 cut(s) 990
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 64, 890
BspMAI CTGCAG 1 cut(s) 1147
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 5 cut(s) 622, 694, 761, 883, 896
BsrDI GCAATG 2 cut(s) 429, 807
BsrFI RCCGGY 1 cut(s) 932
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 4 cut(s) 53, 583, 808, 825
BssMI GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 53, 825
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 4 cut(s) 60, 319, 585, 1133
Bst4CI ACNGT 2 cut(s) 712, 913
Bst6I CTCTTC 2 cut(s) 157, 168
BstACI GRCGYC 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 205, 611, 615
BstDEI CTNAG 3 cut(s) 173, 208, 1083
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 6 cut(s) 511, 745, 869, 921, 1072, 1141
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 8 cut(s) 370, 529, 604, 630, 689, 769, 849, 891
BstMBI GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 4 cut(s) 209, 218, 312, 428
BstNI CCWGG 4 cut(s) 60, 319, 585, 1133
BstNSI RCATGY 2 cut(s) 91, 279
BstSCI CCNGG 4 cut(s) 58, 317, 583, 1131
BstSFI CTRYAG 1 cut(s) 1143
BstSLI GKGCMC 1 cut(s) 706
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 4 cut(s) 25, 557, 703, 748
BstV2I GAAGAC 1 cut(s) 338
BstX2I RGATCY 1 cut(s) 888
BstYI RGATCY 1 cut(s) 888
BsuRI GGCC 3 cut(s) 58, 63, 431
BtgI CCRYGG 1 cut(s) 808
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 6 cut(s) 511, 745, 869, 921, 1072, 1141
BtsI GCAGTG 2 cut(s) 1011, 1140
BtsIMutI CAGTG 4 cut(s) 708, 909, 1011, 1140
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 3 cut(s) 205, 611, 615
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 932
Cfr13I GGNCC 2 cut(s) 62, 242
CseI GACGC 1 cut(s) 390
Csp6I GTAC 4 cut(s) 1048, 1120, 1129, 1159
CspAI ACCGGT 1 cut(s) 932
CspCI CAANNNNNGTGG 2 cut(s) 716, 751
CviAII CATG 7 cut(s) 88, 106, 276, 553, 655, 751, 1099
CviQI GTAC 4 cut(s) 1048, 1120, 1129, 1159
DdeI CTNAG 3 cut(s) 173, 208, 1083
DpnI GATC 8 cut(s) 369, 528, 603, 629, 688, 768, 848, 890
DpnII GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 2 cut(s) 157, 168
EarI CTCTTC 2 cut(s) 157, 168
Ecl136II GAGCTC 2 cut(s) 356, 721
Eco130I CCWWGG 2 cut(s) 53, 825
Eco147I AGGCCT 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 358, 723
Eco47I GGWCC 1 cut(s) 242
Eco53kI GAGCTC 2 cut(s) 356, 721
Eco57I CTGAAG 3 cut(s) 501, 780, 959
EcoICRI GAGCTC 2 cut(s) 356, 721
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 4 cut(s) 58, 317, 583, 1131
EcoT14I CCWWGG 2 cut(s) 53, 825
EcoT38I GRGCYC 2 cut(s) 358, 723
ErhI CCWWGG 2 cut(s) 53, 825
FaeI CATG 7 cut(s) 91, 109, 279, 556, 658, 754, 1102
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FatI CATG 7 cut(s) 87, 105, 275, 552, 654, 750, 1098
FauI CCCGC 1 cut(s) 505
FbaI TGATCA 1 cut(s) 846
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 6 cut(s) 39, 290, 432, 571, 692, 762
FokI GGATG 6 cut(s) 498, 752, 856, 908, 1079, 1148
FriOI GRGCYC 2 cut(s) 358, 723
Fsp4HI GCNGC 6 cut(s) 39, 290, 432, 571, 692, 762
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 316
GluI GCNGC 6 cut(s) 39, 290, 432, 571, 692, 762
GsaI CCCAGC 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 3 cut(s) 58, 63, 431
HapII CCGG 2 cut(s) 933, 991
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 7 cut(s) 91, 109, 279, 556, 658, 754, 1102
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 2 cut(s) 30, 111
HindII GTYRAC 2 cut(s) 30, 111
HindIII AAGCTT 2 cut(s) 394, 611
HinfI GANTC 5 cut(s) 253, 259, 265, 283, 532
HpaII CCGG 2 cut(s) 933, 991
HphI GGTGA 3 cut(s) 216, 536, 1009
Hpy166II GTNNAC 6 cut(s) 30, 111, 909, 1011, 1050, 1080
Hpy188III TCNNGA 5 cut(s) 405, 716, 980, 991, 1187
Hpy8I GTNNAC 6 cut(s) 30, 111, 909, 1011, 1050, 1080
HpyAV CCTTC 4 cut(s) 166, 525, 584, 1148
HpyCH4III ACNGT 2 cut(s) 712, 913
HpyCH4IV ACGT 1 cut(s) 1182
HpyCH4V TGCA 3 cut(s) 646, 1042, 1145
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 218, 312, 428
HpyF3I CTNAG 3 cut(s) 173, 208, 1083
HpySE526I ACGT 1 cut(s) 1182
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 7 cut(s) 91, 109, 279, 556, 658, 754, 1102
HspAI GCGC 1 cut(s) 315
Kpn2I TCCGGA 1 cut(s) 990
Ksp22I TGATCA 1 cut(s) 846
Kzo9I GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
LmnI GCTCC 2 cut(s) 103, 673
Lsp1109I GCAGC 4 cut(s) 25, 557, 703, 748
LweI GCATC 3 cut(s) 230, 730, 1126
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 1182
MaeIII GTNAC 3 cut(s) 562, 773, 917
MalI GATC 8 cut(s) 369, 528, 603, 629, 688, 768, 848, 890
MboI GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
MboII GAAGA 9 cut(s) 144, 155, 185, 288, 338, 341, 643, 952, 1181
MflI RGATCY 1 cut(s) 888
MhlI GDGCHC 4 cut(s) 358, 706, 723, 824
MluCI AATT 3 cut(s) 360, 409, 865
MlyI GAGTC 4 cut(s) 247, 253, 259, 277
MmeI TCCRAC 1 cut(s) 260
MroI TCCGGA 1 cut(s) 990
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 1016
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 2 cut(s) 933, 991
MspR9I CCNGG 4 cut(s) 60, 319, 585, 1133
MvaI CCWGG 4 cut(s) 60, 319, 585, 1133
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 4 cut(s) 209, 218, 312, 428
NdeII GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
NlaIII CATG 7 cut(s) 91, 109, 279, 556, 658, 754, 1102
NlaIV GGNNCC 2 cut(s) 64, 890
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 773, 917
NspI RCATGY 2 cut(s) 91, 279
PceI AGGCCT 1 cut(s) 58
PciI ACATGT 2 cut(s) 87, 275
PcsI WCGNNNNNNNCGW 1 cut(s) 349
PfeI GAWTC 1 cut(s) 532
PflMI CCANNNNNTGG 1 cut(s) 59
PinAI ACCGGT 1 cut(s) 932
PkrI GCNGC 6 cut(s) 40, 291, 433, 572, 693, 763
Ple19I CGATCG 1 cut(s) 370
PleI GAGTC 4 cut(s) 247, 253, 259, 277
PpsI GAGTC 4 cut(s) 247, 253, 259, 277
PscI ACATGT 2 cut(s) 87, 275
Psp124BI GAGCTC 2 cut(s) 358, 723
Psp6I CCWGG 4 cut(s) 58, 317, 583, 1131
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 4 cut(s) 58, 317, 583, 1131
PspN4I GGNNCC 2 cut(s) 64, 890
PspPI GGNCC 2 cut(s) 62, 242
PstI CTGCAG 1 cut(s) 1147
PsuI RGATCY 1 cut(s) 888
PvuI CGATCG 1 cut(s) 370
RgaI GCGATCGC 1 cut(s) 370
RsaI GTAC 4 cut(s) 1049, 1121, 1130, 1160
RsaNI GTAC 4 cut(s) 1048, 1120, 1129, 1159
RseI CAYNNNNRTG 1 cut(s) 1016
SacI GAGCTC 2 cut(s) 358, 723
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 6 cut(s) 39, 290, 432, 571, 692, 762
Sau3AI GATC 8 cut(s) 367, 526, 601, 627, 686, 766, 846, 888
Sau96I GGNCC 2 cut(s) 62, 242
SchI GAGTC 4 cut(s) 247, 253, 259, 277
ScrFI CCNGG 4 cut(s) 60, 319, 585, 1133
SduI GDGCHC 4 cut(s) 358, 706, 723, 824
SfaAI GCGATCGC 1 cut(s) 370
SfaNI GCATC 3 cut(s) 230, 730, 1126
SfcI CTRYAG 1 cut(s) 1143
SgfI GCGATCGC 1 cut(s) 370
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 1016
Sse9I AATT 3 cut(s) 360, 409, 865
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 8 cut(s) 36, 289, 389, 432, 498, 607, 781, 1024
SspMI CTAG 1 cut(s) 501
SstI GAGCTC 2 cut(s) 358, 723
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 4 cut(s) 58, 317, 583, 1131
StyI CCWWGG 2 cut(s) 53, 825
TaaI ACNGT 2 cut(s) 712, 913
TaiI ACGT 1 cut(s) 1185
TaqI TCGA 7 cut(s) 29, 251, 299, 352, 413, 477, 950
TasI AATT 3 cut(s) 360, 409, 865
TauI GCSGC 2 cut(s) 292, 434
TfiI GAWTC 1 cut(s) 532
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 4 cut(s) 715, 916, 1018, 1147
TseFI GTSAC 2 cut(s) 773, 917
TseI GCWGC 4 cut(s) 38, 570, 691, 761
Tsp45I GTSAC 2 cut(s) 773, 917
TspDTI ATGAA 7 cut(s) 476, 643, 852, 858, 946, 1115, 1182
TspGWI ACGGA 2 cut(s) 13, 797
TspRI CASTG 4 cut(s) 715, 916, 1018, 1147
Van91I CCANNNNNTGG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 2 cut(s) 409, 865
XceI RCATGY 2 cut(s) 91, 279
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.