Rh1CG347900

NADH-cytochrome b5 reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0017766
Physical Location & Seq
Forward (+)
62488079 .. 62501488
13410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG347900.1

Sequence Viewer

Length: 696 bp
ATGGAAGGAGCAGTGCTTCGGTTTGCGGCGGAGACTCTGGTGGACAAGGCCATGGAGCTCGATCACATCGGCGGGACCTATATGTATCTCTCTAGATTCCATGCACAAGAGATGAAGGAGACTGACTTTGATAATTTGGGGGTTGCGGTAATGCTGCACAAGGTAAAAACTTTTGGTTTCATTCTGTTACAATGCCTTTTAAAAGCTAGCAAGTCAGTTACAATTTGTGTCTCGCCATATATGGATTCGCCTTTTGATTTTGGTTGCTTTGTTCTTGGTGCAGCTATTGTTCAGTTGCACTTGACTGATTGTTCAGATTATGTGCATCGGTCAACACAATATCAAACATCCTGTAGTAGTCCTAAAGTCCCATTGCATGGAATCCCTGGTGAAAGCAAGGTTTATGTTGGTAAAACTGGGCAGGAATTGACAGGTCAAACAGCCCACAGACTATGCTCAAAGATGTATGCTCAAGGAAGAATGTCGCACCATTTTCGTGAGATGTGTGTTGGAGATCATGTTGCTGTCAAGGGACCCAAGGACCGTTTCAAGTATGAGCCTGGCCAAGTTAGAACTGTTGGGATGCTTGGTAGAGGCTCTCGAATCACTCTGATGTTCCAGGTTGCTAGGGCAATATTAGAAAATCCAAAAGACAAAACAAGGGTTCTCCTAATCTATGTCAATTGTGACCTATGA

Protein Analysis

231

Amino Acids

25.8

Weight (kDa)

8.73

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_6 PF00970 150 - 185 6.7e-06 Oxidoreductase FAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 377
AciI CCGC 4 cut(s) 26, 29, 72, 146
AcoI YGGCCR 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 1 cut(s) 550
AjnI CCWGG 3 cut(s) 385, 559, 618
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 3 cut(s) 58, 206, 284
AluI AGCT 3 cut(s) 58, 206, 284
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 3 cut(s) 26, 113, 235
AoxI GGCC 2 cut(s) 48, 562
ApeKI GCWGC 2 cut(s) 154, 281
AspS9I GGNCC 3 cut(s) 75, 533, 541
AsuHPI GGTGA 1 cut(s) 401
AsuNHI GCTAGC 1 cut(s) 206
AvaII GGWCC 3 cut(s) 75, 533, 541
BalI TGGCCA 1 cut(s) 564
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 141, 293
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 3 cut(s) 387, 561, 620
BcoDI GTCTC 3 cut(s) 26, 113, 235
BfaI CTAG 3 cut(s) 93, 207, 627
BfmI CTRYAG 1 cut(s) 352
BisI GCNGC 3 cut(s) 27, 155, 282
BlsI GCNGC 3 cut(s) 28, 156, 283
Bme1390I CCNGG 3 cut(s) 387, 561, 620
Bme18I GGWCC 3 cut(s) 75, 533, 541
BmgT120I GGNCC 3 cut(s) 75, 533, 541
BmiI GGNNCC 3 cut(s) 76, 534, 535
BmrFI CCNGG 3 cut(s) 387, 561, 620
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 334, 573
BmtI GCTAGC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 51, 385, 537
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 421
Bse3DI GCAATG 1 cut(s) 371
BseBI CCWGG 3 cut(s) 387, 561, 620
BseDI CCNNGG 3 cut(s) 51, 385, 537
BseGI GGATG 2 cut(s) 347, 588
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMI GCAATG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 421
BseXI GCAGC 2 cut(s) 141, 293
BsgI GTGCAG 2 cut(s) 140, 300
BshFI GGCC 2 cut(s) 50, 564
BsiHKAI GWGCWC 1 cut(s) 60
BslFI GGGAC 3 cut(s) 88, 353, 546
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 3 cut(s) 26, 113, 235
BsmFI GGGAC 3 cut(s) 88, 353, 546
BsnI GGCC 2 cut(s) 50, 564
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 2 cut(s) 61, 514
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 4 cut(s) 26, 29, 72, 146
BspANI GGCC 2 cut(s) 50, 564
BspLI GGNNCC 3 cut(s) 76, 534, 535
BspOI GCTAGC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 371
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 3 cut(s) 51, 385, 537
BssMI GATC 2 cut(s) 61, 514
BssT1I CCWWGG 2 cut(s) 51, 537
Bst2UI CCWGG 3 cut(s) 387, 561, 620
Bst4CI ACNGT 2 cut(s) 545, 577
BstC8I GCNNGC 1 cut(s) 208
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 347, 588
BstKTI GATC 2 cut(s) 64, 517
BstMAI GTCTC 3 cut(s) 26, 113, 235
BstMBI GATC 2 cut(s) 61, 514
BstNI CCWGG 3 cut(s) 387, 561, 620
BstSCI CCNGG 3 cut(s) 385, 559, 618
BstSFI CTRYAG 1 cut(s) 352
BstV1I GCAGC 2 cut(s) 141, 293
BsuRI GGCC 2 cut(s) 50, 564
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 347, 588
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 208
Cfr13I GGNCC 3 cut(s) 75, 533, 541
CviAII CATG 4 cut(s) 52, 101, 377, 518
CviJI RGCY 8 cut(s) 50, 58, 206, 284, 443, 559, 564, 597
CviKI_1 RGCY 8 cut(s) 50, 58, 206, 284, 443, 559, 564, 597
DpnI GATC 2 cut(s) 63, 516
DpnII GATC 2 cut(s) 61, 514
DraI TTTAAA 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 562
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 51, 537
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 3 cut(s) 75, 533, 541
Eco53kI GAGCTC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 2 cut(s) 75, 533
EcoRII CCWGG 3 cut(s) 385, 559, 618
EcoT14I CCWWGG 2 cut(s) 51, 537
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 51, 537
FaeI CATG 4 cut(s) 55, 104, 380, 521
FaqI GGGAC 3 cut(s) 88, 353, 546
FatI CATG 4 cut(s) 51, 100, 376, 517
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 3 cut(s) 27, 155, 282
FokI GGATG 2 cut(s) 334, 595
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 3 cut(s) 27, 155, 282
FspBI CTAG 3 cut(s) 93, 207, 627
GluI GCNGC 3 cut(s) 27, 155, 282
HaeIII GGCC 2 cut(s) 50, 564
Hin1II CATG 4 cut(s) 55, 104, 380, 521
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 5 cut(s) 34, 96, 245, 381, 603
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 43, 333
Hpy188I TCNGA 2 cut(s) 316, 612
Hpy188III TCNNGA 3 cut(s) 93, 497, 600
Hpy8I GTNNAC 2 cut(s) 43, 333
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 2 cut(s) 545, 577
HpyCH4V TGCA 6 cut(s) 104, 157, 281, 298, 325, 376
Hsp92II CATG 4 cut(s) 55, 104, 380, 521
KflI GGGWCCC 1 cut(s) 533
Kzo9I GATC 2 cut(s) 61, 514
LmnI GCTCC 2 cut(s) 8, 55
Lsp1109I GCAGC 2 cut(s) 141, 293
LweI GCATC 2 cut(s) 334, 573
MaeI CTAG 3 cut(s) 93, 207, 627
MaeIII GTNAC 3 cut(s) 186, 217, 686
MalI GATC 2 cut(s) 63, 516
MboI GATC 2 cut(s) 61, 514
MboII GAAGA 1 cut(s) 489
MfeI CAATTG 1 cut(s) 682
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 4 cut(s) 133, 222, 425, 682
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 1 cut(s) 587
Mox20I TGGCCA 1 cut(s) 564
MscI TGGCCA 1 cut(s) 564
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 2 cut(s) 495, 611
Msp20I TGGCCA 1 cut(s) 564
MspR9I CCNGG 3 cut(s) 387, 561, 620
MunI CAATTG 1 cut(s) 682
MvaI CCWGG 3 cut(s) 387, 561, 620
NcoI CCATGG 1 cut(s) 51
NdeII GATC 2 cut(s) 61, 514
NheI GCTAGC 1 cut(s) 206
NlaIII CATG 4 cut(s) 55, 104, 380, 521
NlaIV GGNNCC 3 cut(s) 76, 534, 535
NmuCI GTSAC 1 cut(s) 686
PfeI GAWTC 4 cut(s) 96, 245, 381, 603
PflMI CCANNNNNTGG 1 cut(s) 377
PkrI GCNGC 3 cut(s) 28, 156, 283
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 2 cut(s) 75, 533
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 2 cut(s) 75, 533
Psp6I CCWGG 3 cut(s) 385, 559, 618
PspGI CCWGG 3 cut(s) 385, 559, 618
PspN4I GGNNCC 3 cut(s) 76, 534, 535
PspPI GGNCC 3 cut(s) 75, 533, 541
PspPPI RGGWCCY 2 cut(s) 75, 533
RseI CAYNNNNRTG 2 cut(s) 495, 611
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 3 cut(s) 27, 155, 282
Sau3AI GATC 2 cut(s) 61, 514
Sau96I GGNCC 3 cut(s) 75, 533, 541
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 3 cut(s) 387, 561, 620
SduI GDGCHC 1 cut(s) 60
SetI ASST 9 cut(s) 60, 80, 165, 208, 286, 402, 436, 624, 693
SfaNI GCATC 2 cut(s) 334, 573
SfcI CTRYAG 1 cut(s) 352
SinI GGWCC 3 cut(s) 75, 533, 541
SmiMI CAYNNNNRTG 2 cut(s) 495, 611
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 4 cut(s) 133, 222, 425, 682
SsiI CCGC 4 cut(s) 26, 29, 72, 146
SspI AATATT 1 cut(s) 636
SspMI CTAG 3 cut(s) 93, 207, 627
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 385, 559, 618
StyI CCWWGG 2 cut(s) 51, 537
TaaI ACNGT 2 cut(s) 545, 577
TaqI TCGA 2 cut(s) 60, 601
TaqII GACCGA 1 cut(s) 318
TasI AATT 4 cut(s) 133, 222, 425, 682
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 4 cut(s) 96, 245, 381, 603
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 2 cut(s) 154, 281
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 2 cut(s) 128, 169
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 75, 533, 541
XbaI TCTAGA 1 cut(s) 92
XspI CTAG 3 cut(s) 93, 207, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.