Rh1DG365800

NADH-cytochrome b5 reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017766
Physical Location & Seq
Forward (+)
59153348 .. 59165748
12401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG365800.1

Sequence Viewer

Length: 360 bp
ATGTTCCAGCTTTTGTGGTCTGTACTTGGTCGTCATTGGTATACAGTCCCTGGTGAAAGCAAGGTTTATGTTGGTAAAACTGGGCAGGAATTGACAGGTCAAACAGCCCACAGACTATGCTCAAAGATGTATGCTCAAGGAAGAATGTCGCACCATTTTCGTGAGATGTGTGTTGGAGATCATGTTGCTGTCAAGGGACCCAAGGACCGTTTCAAGTATGAGCCTGGCCAAGTTAGAACTGTTGGGATGCTTGGTAGAGGCTCTCGAATCACTCTGATGTTCCAGGTTGCTAGGGCAATATTAGAAAATCCAAAAGACAAAACAAGGGTTCTCCTAATCTATGTCAATTGTGACCTATGA

Protein Analysis

119

Amino Acids

13.61

Weight (kDa)

9.84

Isoelectric Point (pI)

19.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_6 PF00970 38 - 73 1.9e-06 Oxidoreductase FAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 226
AfaI GTAC 1 cut(s) 24
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 3 cut(s) 49, 223, 282
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 197, 205
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 2 cut(s) 197, 205
BalI TGGCCA 1 cut(s) 228
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 3 cut(s) 51, 225, 284
BfaI CTAG 1 cut(s) 291
Bme1390I CCNGG 3 cut(s) 51, 225, 284
Bme18I GGWCC 2 cut(s) 197, 205
BmgT120I GGNCC 2 cut(s) 197, 205
BmiI GGNNCC 2 cut(s) 198, 199
BmrFI CCNGG 3 cut(s) 51, 225, 284
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 49, 201
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 3 cut(s) 51, 225, 284
BseDI CCNNGG 2 cut(s) 49, 201
BseGI GGATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 85
BshFI GGCC 1 cut(s) 228
BslFI GGGAC 2 cut(s) 32, 210
BsmFI GGGAC 2 cut(s) 32, 210
BsnI GGCC 1 cut(s) 228
Bsp143I GATC 1 cut(s) 178
BspANI GGCC 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 198, 199
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 49, 201
BssMI GATC 1 cut(s) 178
BssNAI GTATAC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 51, 225, 284
Bst4CI ACNGT 3 cut(s) 46, 209, 241
BstF5I GGATG 1 cut(s) 252
BstKTI GATC 1 cut(s) 181
BstMBI GATC 1 cut(s) 178
BstNI CCWGG 3 cut(s) 51, 225, 284
BstSCI CCNGG 3 cut(s) 49, 223, 282
BstZ17I GTATAC 1 cut(s) 42
BsuRI GGCC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 252
CaiI CAGNNNCTG 1 cut(s) 50
Cfr13I GGNCC 2 cut(s) 197, 205
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 182
CviJI RGCY 5 cut(s) 10, 107, 223, 228, 261
CviKI_1 RGCY 5 cut(s) 10, 107, 223, 228, 261
CviQI GTAC 1 cut(s) 23
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
EaeI YGGCCR 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 197, 205
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 49, 223, 282
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 1 cut(s) 185
FaiI YATR 8 cut(s) 42, 69, 118, 132, 183, 219, 342, 358
FaqI GGGAC 2 cut(s) 32, 210
FatI CATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 41
FokI GGATG 1 cut(s) 259
FspBI CTAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 228
Hin1II CATG 1 cut(s) 185
HinfI GANTC 1 cut(s) 267
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 1 cut(s) 276
Hpy188III TCNNGA 2 cut(s) 161, 264
Hpy8I GTNNAC 1 cut(s) 42
HpyCH4III ACNGT 3 cut(s) 46, 209, 241
Hsp92II CATG 1 cut(s) 185
KflI GGGWCCC 1 cut(s) 197
Kzo9I GATC 1 cut(s) 178
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 350
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 1 cut(s) 153
MfeI CAATTG 1 cut(s) 346
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 2 cut(s) 89, 346
MluNI TGGCCA 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 1 cut(s) 251
Mox20I TGGCCA 1 cut(s) 228
MscI TGGCCA 1 cut(s) 228
MslI CAYNNNNRTG 2 cut(s) 159, 275
Msp20I TGGCCA 1 cut(s) 228
MspR9I CCNGG 3 cut(s) 51, 225, 284
MunI CAATTG 1 cut(s) 346
MvaI CCWGG 3 cut(s) 51, 225, 284
NdeII GATC 1 cut(s) 178
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 2 cut(s) 198, 199
NmuCI GTSAC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 267
PpuMI RGGWCCY 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 49, 223, 282
PspGI CCWGG 3 cut(s) 49, 223, 282
PspN4I GGNNCC 2 cut(s) 198, 199
PspPI GGNCC 2 cut(s) 197, 205
PspPPI RGGWCCY 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 159, 275
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 2 cut(s) 197, 205
ScrFI CCNGG 3 cut(s) 51, 225, 284
SetI ASST 5 cut(s) 12, 66, 100, 288, 357
SfaNI GCATC 1 cut(s) 237
SinI GGWCC 2 cut(s) 197, 205
SmiMI CAYNNNNRTG 2 cut(s) 159, 275
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 2 cut(s) 89, 346
SspI AATATT 1 cut(s) 300
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 49, 223, 282
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 46, 209, 241
TaqI TCGA 1 cut(s) 265
TasI AATT 2 cut(s) 89, 346
TatI WGTACW 1 cut(s) 22
TfiI GAWTC 1 cut(s) 267
TseFI GTSAC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 350
VpaK11BI GGWCC 2 cut(s) 197, 205
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.