Rh1DG420200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0020690
Physical Location & Seq
Forward (+)
63468020 .. 63469269
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG420200.1

Sequence Viewer

Length: 366 bp
ATGTTGCAGAACAAGGAAGAGGATGAGTTTATGGTCAATGTTGGGAAGGACGACAAGAAAGAGGATGAGGTTGTGGTCAAGGATGACAATGGCAAGGAAGATGAGGCTAAAGATGGAAATGAGTCTTCAAATCAGAGCAGGAGTGAGAAGAAGAGTTGTAAGGATATCTGGAGTTTGTCATCCAACATCGATTTCACATATGTTGAGGAGTTGAGATCTCAGATCAGTCAGAAGCTGACACAGGCTGCCCAGCGGGGAAAACCAGAACGTTTCGAAGCTGGTACTGGAGCTCAGGCATATAAAGAGCTGGAAGAGGTTGATGAGCCTGCGGACATTGATTTTTTCATGACACAGGCCGGGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.72

Weight (kDa)

4.41

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020690)

Species Orthologous Gene IDs
rosa_laevigata RLG00000026424 RLG00000026428
rosa_roxburghii Rroxscaffold_4G00280400
rosa_samantha Rh1BG389000 Rh1CG402400 Rh1DG420200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 364
AciI CCGC 2 cut(s) 253, 329
AclI AACGTT 1 cut(s) 268
AfaI GTAC 1 cut(s) 283
AgsI TTSAA 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 4 cut(s) 235, 278, 290, 307
AluI AGCT 4 cut(s) 235, 278, 290, 307
Alw21I GWGCWC 1 cut(s) 292
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 358
AsuII TTCGAA 1 cut(s) 273
BanII GRGCYC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 107
BcnI CCSGG 1 cut(s) 358
BglII AGATCT 1 cut(s) 215
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 117
BpmI CTGGAG 2 cut(s) 190, 306
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 289
BseCI ATCGAT 1 cut(s) 189
BseGI GGATG 4 cut(s) 28, 70, 88, 179
BseMII CTCAG 2 cut(s) 233, 305
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 249
BshFI GGCC 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 1 cut(s) 357
BsnI GGCC 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 2 cut(s) 215, 222
BspACI CCGC 2 cut(s) 253, 329
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 2 cut(s) 232, 304
BspDI ATCGAT 1 cut(s) 189
BspHI TCATGA 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 289
BssMI GATC 2 cut(s) 215, 222
Bst6I CTCTTC 3 cut(s) 12, 146, 306
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 2 cut(s) 219, 291
BstF5I GGATG 4 cut(s) 28, 70, 88, 179
BstKTI GATC 2 cut(s) 218, 225
BstMBI GATC 2 cut(s) 215, 222
BstSCI CCNGG 1 cut(s) 356
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 1 cut(s) 356
BsuTUI ATCGAT 1 cut(s) 189
BtsCI GGATG 4 cut(s) 28, 70, 88, 179
Cac8I GCNNGC 1 cut(s) 327
CaiI CAGNNNCTG 1 cut(s) 235
CciI TCATGA 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 1 cut(s) 282
CviAII CATG 1 cut(s) 346
CviJI RGCY 8 cut(s) 107, 235, 245, 278, 290, 307, 325, 356
CviKI_1 RGCY 8 cut(s) 107, 235, 245, 278, 290, 307, 325, 356
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 2 cut(s) 219, 291
DpnI GATC 2 cut(s) 217, 224
DpnII GATC 2 cut(s) 215, 222
Eam1104I CTCTTC 3 cut(s) 12, 146, 306
EarI CTCTTC 3 cut(s) 12, 146, 306
Ecl136II GAGCTC 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 290
EcoRV GATATC 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 292
FaeI CATG 1 cut(s) 349
FaiI YATR 7 cut(s) 32, 199, 201, 298, 300, 347, 364
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 246
FauNDI CATATG 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 4 cut(s) 35, 77, 95, 166
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 1 cut(s) 246
GluI GCNGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 253
GsuI CTGGAG 2 cut(s) 190, 306
HaeIII GGCC 1 cut(s) 356
HapII CCGG 1 cut(s) 357
Hin1II CATG 1 cut(s) 349
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 357
Hpy188I TCNGA 3 cut(s) 135, 222, 231
Hpy188III TCNNGA 2 cut(s) 169, 346
HpyAV CCTTC 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 7
HpyF3I CTNAG 2 cut(s) 219, 291
HpySE526I ACGT 1 cut(s) 268
Hsp92II CATG 1 cut(s) 349
Kzo9I GATC 2 cut(s) 215, 222
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 1 cut(s) 232
MaeII ACGT 1 cut(s) 268
MalI GATC 2 cut(s) 217, 224
MboI GATC 2 cut(s) 215, 222
MboII GAAGA 6 cut(s) 29, 110, 117, 160, 163, 323
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 292
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 6 cut(s) 13, 55, 61, 97, 199, 307
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 358
NciI CCSGG 1 cut(s) 358
NdeI CATATG 1 cut(s) 199
NdeII GATC 2 cut(s) 215, 222
NlaIII CATG 1 cut(s) 349
NspV TTCGAA 1 cut(s) 273
PagI TCATGA 1 cut(s) 345
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PsiI TTATAA 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 292
Psp1406I AACGTT 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 249
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SacI GAGCTC 1 cut(s) 292
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 2 cut(s) 215, 222
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 292
SetI ASST 7 cut(s) 72, 237, 271, 280, 292, 309, 318
SfuI TTCGAA 1 cut(s) 273
SsiI CCGC 2 cut(s) 253, 329
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 1 cut(s) 356
TaiI ACGT 1 cut(s) 271
TaqI TCGA 2 cut(s) 189, 273
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.