Rh2AG323000

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019776
Physical Location & Seq
Reverse (-)
46422714 .. 46424189
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG323000.1

Sequence Viewer

Length: 861 bp
ATGGAGGGATATGAACCTAAAGTCCCAAAAGAGTGGGGATTAGGGGATCACACAGATGGGTGTGTCAGGAAAGCACCTTTAAAATGCATTGCTGGAGTAGATGATACTTTTAAGGTGGTATCTGATGTCCGCCTTCCTGTGAATCCAGAGACTTTAGCAGTTGATAACAGTGATGATTGCAGATTAGCGTGCTTAAGAAATTGCTTATGTACAGCTTTTGCTTATGATAATAGGTGTCTTGTTTGGGGAGGGGATCTATTCAACGTCAAACAATTTACACCAGACGAGAAGGAAGGCAAGGACTTGCATCTGAAAATTCCAGCTTCTGAGAGAATAGAGGGGAAGAAAGAGAACAGGACTCTTTGGATCATCATCGGAGTACTTGGAGGTTTATTTATTGTTATTACCATTGTTGTGGTACTATTTGTGAAGCATCGATGTTTTGGGGAAAAGTTAGATACACTTGATGGTTCCTTGGTGATGTTCAGGAGAAACAGAGATCTATTAGATGATGCTTTAGAAAATTACTTCCCAACCCGTGTTGCCAATGTGTTGAATAAAGAAGAAGATGTTGATGCCTTGTTGGATTACAGGTTAGAAGGTAATGCTGACAAAGACCAACTGAGTAGAGCATGCAAAGTCGCTTGTTGGTGCATTCAAGACGACGAGAAGGGTAGGCCAACTATGGGGCAGGTTGTTCAACTTCTGGAGGGAGTTATAGATGCTGGAATTCCACCAATGCCAAAGTTCCTTGACCGCTTTTCTAAGAGTCTAGTTGAATCCATCAATTATCGGTACATCACTTCCAGCAGTTCTGATTCATGTCGTCATGAGTACTCAGATAGTATATATCAAGCTTAA

Protein Analysis

286

Amino Acids

32.23

Weight (kDa)

5.11

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 32 - 89 6e-13 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019776)

Species Orthologous Gene IDs
malus_domestica MD17G1108100.v1.1
rosa_chinensis RchiOBHm_Chr2g0127781
rosa_roxburghii Rroxscaffold_175G00432500 Rroxscaffold_7G00202500
rosa_samantha Rh2AG323000 Rh2AG323200 Rh2BG332400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 682
AciI CCGC 2 cut(s) 130, 757
AclWI GGATC 3 cut(s) 54, 261, 374
AcsI RAATTY 2 cut(s) 315, 729
AfaI GTAC 5 cut(s) 211, 381, 420, 797, 836
AfiI CCNNNNNNNGG 1 cut(s) 686
AflII CTTAAG 1 cut(s) 193
AgsI TTSAA 5 cut(s) 262, 556, 659, 701, 779
AloI GAACNNNNNNTCC 1 cut(s) 38
AluBI AGCT 3 cut(s) 215, 323, 857
AluI AGCT 3 cut(s) 215, 323, 857
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 3 cut(s) 54, 261, 374
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 1 cut(s) 677
ApoI RAATTY 2 cut(s) 315, 729
AsuHPI GGTGA 1 cut(s) 490
BccI CCATC 3 cut(s) 50, 461, 791
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 773
BfrI CTTAAG 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 682
BglII AGATCT 1 cut(s) 499
BmcAI AGTACT 2 cut(s) 381, 836
BmiI GGNNCC 1 cut(s) 472
BmsI GCATC 5 cut(s) 316, 442, 502, 565, 712
BpmI CTGGAG 2 cut(s) 114, 728
Bsa29I ATCGAT 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 87
Bse8I GATNNNNATC 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 474
BseJI GATNNNNATC 1 cut(s) 371
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 3 cut(s) 318, 614, 852
BshFI GGCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 436
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 8
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 1 cut(s) 679
Bsp1407I TGTACA 1 cut(s) 209
Bsp143I GATC 4 cut(s) 46, 253, 366, 499
BspACI CCGC 2 cut(s) 130, 757
BspANI GGCC 1 cut(s) 679
BspCNI CTCAG 3 cut(s) 319, 615, 851
BspDI ATCGAT 1 cut(s) 436
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 472
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 3 cut(s) 54, 261, 374
BspTI CTTAAG 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 4 cut(s) 46, 253, 366, 499
BssT1I CCWWGG 1 cut(s) 474
Bst4CI ACNGT 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 193
BstAUI TGTACA 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 190, 634
BstDEI CTNAG 4 cut(s) 327, 623, 765, 838
BstKTI GATC 4 cut(s) 49, 256, 369, 502
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 4 cut(s) 46, 253, 366, 499
BstNSI RCATGY 1 cut(s) 636
BstX2I RGATCY 2 cut(s) 253, 499
BstXI CCANNNNNNTGG 2 cut(s) 33, 415
BstYI RGATCY 2 cut(s) 253, 499
Bsu15I ATCGAT 1 cut(s) 436
BsuRI GGCC 1 cut(s) 679
BsuTUI ATCGAT 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 2 cut(s) 190, 634
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 829
ClaI ATCGAT 1 cut(s) 436
Csp6I GTAC 5 cut(s) 210, 380, 419, 796, 835
CviAII CATG 3 cut(s) 633, 822, 830
CviJI RGCY 4 cut(s) 215, 323, 679, 857
CviKI_1 RGCY 4 cut(s) 215, 323, 679, 857
CviQI GTAC 5 cut(s) 210, 380, 419, 796, 835
DdeI CTNAG 4 cut(s) 327, 623, 765, 838
DpnI GATC 4 cut(s) 48, 255, 368, 501
DpnII GATC 4 cut(s) 46, 253, 366, 499
DraI TTTAAA 1 cut(s) 81
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 474
EcoRI GAATTC 1 cut(s) 729
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 3 cut(s) 636, 825, 833
FaqI GGGAC 1 cut(s) 8
FatI CATG 3 cut(s) 632, 821, 829
FspBI CTAG 1 cut(s) 773
GsuI CTGGAG 2 cut(s) 114, 728
HaeIII GGCC 1 cut(s) 679
Hin1II CATG 3 cut(s) 636, 825, 833
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 5 cut(s) 142, 358, 769, 779, 818
HphI GGTGA 1 cut(s) 490
Hpy188I TCNGA 6 cut(s) 124, 312, 328, 377, 817, 841
Hpy188III TCNNGA 6 cut(s) 67, 146, 487, 659, 707, 830
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 5 cut(s) 143, 283, 287, 593, 664
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 87, 180, 307, 636, 654
HpyF3I CTNAG 4 cut(s) 327, 623, 765, 838
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 3 cut(s) 636, 825, 833
Kzo9I GATC 4 cut(s) 46, 253, 366, 499
LweI GCATC 5 cut(s) 316, 442, 502, 565, 712
MaeI CTAG 1 cut(s) 773
MaeII ACGT 1 cut(s) 264
MalI GATC 4 cut(s) 48, 255, 368, 501
MboI GATC 4 cut(s) 46, 253, 366, 499
MboII GAAGA 3 cut(s) 355, 575, 578
MflI RGATCY 2 cut(s) 253, 499
MluCI AATT 6 cut(s) 199, 272, 315, 523, 729, 787
MlyI GAGTC 2 cut(s) 352, 778
MmeI TCCRAC 1 cut(s) 564
MnlI CCTC 4 cut(s) 242, 331, 380, 703
Mph1103I ATGCAT 1 cut(s) 89
MseI TTAA 4 cut(s) 80, 111, 194, 859
MslI CAYNNNNRTG 2 cut(s) 54, 413
MspCI CTTAAG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 654
NdeII GATC 4 cut(s) 46, 253, 366, 499
NlaIII CATG 3 cut(s) 636, 825, 833
NlaIV GGNNCC 1 cut(s) 472
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 636
PaeI GCATGC 1 cut(s) 636
PagI TCATGA 1 cut(s) 829
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 3 cut(s) 142, 779, 818
PleI GAGTC 2 cut(s) 352, 777
PpsI GAGTC 2 cut(s) 352, 777
PspN4I GGNNCC 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 253, 499
RsaI GTAC 5 cut(s) 211, 381, 420, 797, 836
RsaNI GTAC 5 cut(s) 210, 380, 419, 796, 835
RseI CAYNNNNRTG 2 cut(s) 54, 413
SaqAI TTAA 4 cut(s) 80, 111, 194, 859
Sau3AI GATC 4 cut(s) 46, 253, 366, 499
ScaI AGTACT 2 cut(s) 381, 836
SchI GAGTC 2 cut(s) 352, 778
SfaNI GCATC 5 cut(s) 316, 442, 502, 565, 712
SmiMI CAYNNNNRTG 2 cut(s) 54, 413
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SphI GCATGC 1 cut(s) 636
Sse9I AATT 6 cut(s) 199, 272, 315, 523, 729, 787
SsiI CCGC 2 cut(s) 130, 757
SspMI CTAG 1 cut(s) 773
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 436
TasI AATT 6 cut(s) 199, 272, 315, 523, 729, 787
TatI WGTACW 3 cut(s) 209, 379, 834
TfiI GAWTC 3 cut(s) 142, 779, 818
Tru1I TTAA 4 cut(s) 80, 111, 194, 859
Tru9I TTAA 4 cut(s) 80, 111, 194, 859
TscAI CASTG 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 27, 810
TspRI CASTG 1 cut(s) 175
Vha464I CTTAAG 1 cut(s) 193
XapI RAATTY 2 cut(s) 315, 729
XceI RCATGY 1 cut(s) 636
XspI CTAG 1 cut(s) 773
ZrmI AGTACT 2 cut(s) 381, 836
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.