Rh2AG626500

acetate--CoA ligase ACS, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0021659
Physical Location & Seq
Reverse (-)
85099602 .. 85099958
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG626500.1

Sequence Viewer

Length: 357 bp
ATGAGCAGGGAAGCGGATGTCGAGGACGACGGCCTTAGCGCTATCCATGATGGTGGTGGCCATGTACCTAGAGTCCTGCTTGCTCCATCCGAGGCAGAACAAGGGGGTGTCGGGATCGGAAGCGAAGACGGAGGCGAAGACACCGACACCGACCGGTTGTCTCTGTTCTGCAGGTGGTTTAATGGAAGAGGCTTCGAATCGGCGTGCCTCACTGCCTCCCATGTCAACTGTGCCCACCCAATATTCAGCCGCGGTTTGGTAACTGCGCGTATCCTACAGACGCACCGTAATGGCTTGGTGCAGGTGGACCTTGGTCCACAGAAGTCTCTTCGAACTTTTGAAACCATAAACAATTAG

Protein Analysis

118

Amino Acids

12.66

Weight (kDa)

5.04

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021659)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002756.1_g000003
rosa_rugosa Rorug06G0204000
rosa_samantha Rh1BG001800 Rh1CG009200 Rh2AG626500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 162, 292
Acc36I ACCTGC 2 cut(s) 162, 292
AccII CGCG 2 cut(s) 252, 268
AciI CCGC 3 cut(s) 14, 250, 252
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 58
AfaI GTAC 1 cut(s) 66
AfeI AGCGCT 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 153
AgsI TTSAA 1 cut(s) 341
AhdI GACNNNNNGTC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 165, 330
AlwI GGATC 1 cut(s) 122
Aor51HI AGCGCT 1 cut(s) 40
AoxI GGCC 2 cut(s) 31, 58
AsiGI ACCGGT 1 cut(s) 153
AspLEI GCGC 2 cut(s) 41, 268
AspS9I GGNCC 2 cut(s) 307, 314
AsuII TTCGAA 2 cut(s) 195, 331
AvaII GGWCC 2 cut(s) 307, 314
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 60
BbsI GAAGAC 2 cut(s) 132, 144
BccI CCATC 2 cut(s) 44, 94
BceAI ACGGC 1 cut(s) 46
BciVI GTATCC 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 165, 330
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 169, 275
BfoI RGCGCY 1 cut(s) 42
BfuAI ACCTGC 2 cut(s) 162, 292
BfuI GTATCC 1 cut(s) 281
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme18I GGWCC 2 cut(s) 307, 314
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 2 cut(s) 307, 314
BoxI GACNNNNGTC 1 cut(s) 312
BpiI GAAGAC 2 cut(s) 132, 144
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 2 cut(s) 195, 331
BsaJI CCNNGG 3 cut(s) 90, 250, 310
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 90, 250, 310
BseGI GGATG 2 cut(s) 22, 86
BseLI CCNNNNNNNGG 1 cut(s) 256
BseSI GKGCMC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 2 cut(s) 252, 268
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 33, 60
BshTI ACCGGT 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 165, 330
BsnI GGCC 2 cut(s) 33, 60
Bsp119I TTCGAA 2 cut(s) 195, 331
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 3 cut(s) 14, 250, 252
BspANI GGCC 2 cut(s) 33, 60
BspFNI CGCG 2 cut(s) 252, 268
BspMAI CTGCAG 1 cut(s) 173
BspMI ACCTGC 2 cut(s) 162, 292
BspPI GGATC 1 cut(s) 122
BspT104I TTCGAA 2 cut(s) 195, 331
BsrFI RCCGGY 1 cut(s) 153
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 3 cut(s) 90, 250, 310
BssMI GATC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 310
Bst4CI ACNGT 2 cut(s) 230, 287
Bst6I CTCTTC 2 cut(s) 181, 333
BstBI TTCGAA 2 cut(s) 195, 331
BstC8I GCNNGC 2 cut(s) 81, 205
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 2 cut(s) 22, 86
BstFNI CGCG 2 cut(s) 252, 268
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 2 cut(s) 41, 268
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 2 cut(s) 165, 330
BstMBI GATC 1 cut(s) 114
BstMCI CGRYCG 1 cut(s) 154
BstPAI GACNNNNGTC 1 cut(s) 312
BstSFI CTRYAG 2 cut(s) 169, 275
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 2 cut(s) 252, 268
BstV2I GAAGAC 2 cut(s) 132, 144
BstXI CCANNNNNNTGG 1 cut(s) 53
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 2 cut(s) 33, 60
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 22, 86
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 210
BveI ACCTGC 2 cut(s) 162, 292
Cac8I GCNNGC 2 cut(s) 81, 205
CfoI GCGC 2 cut(s) 41, 268
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 307, 314
Cfr42I CCGCGG 1 cut(s) 253
CseI GACGC 1 cut(s) 289
Csp6I GTAC 1 cut(s) 65
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 3 cut(s) 47, 62, 221
CviJI RGCY 5 cut(s) 33, 60, 192, 249, 294
CviKI_1 RGCY 5 cut(s) 33, 60, 192, 249, 294
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 181, 333
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 2 cut(s) 181, 333
Eco130I CCWWGG 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 307, 314
Eco47III AGCGCT 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 3 cut(s) 50, 65, 224
FaiI YATR 4 cut(s) 48, 63, 222, 347
FatI CATG 3 cut(s) 46, 61, 220
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 2 cut(s) 29, 73
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 1 cut(s) 69
GlaI GCGC 2 cut(s) 40, 267
GluI GCNGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 2 cut(s) 33, 60
HapII CCGG 1 cut(s) 154
HgaI GACGC 1 cut(s) 289
HhaI GCGC 2 cut(s) 41, 268
Hin1II CATG 3 cut(s) 50, 65, 224
Hin6I GCGC 2 cut(s) 39, 266
HinP1I GCGC 2 cut(s) 39, 266
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 2 cut(s) 72, 197
HpaII CCGG 1 cut(s) 154
Hpy166II GTNNAC 3 cut(s) 226, 307, 317
Hpy188I TCNGA 2 cut(s) 91, 119
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 3 cut(s) 226, 307, 317
Hpy99I CGWCG 1 cut(s) 32
HpyCH4III ACNGT 2 cut(s) 230, 287
HpyCH4V TGCA 2 cut(s) 171, 301
HpyF3I CTNAG 1 cut(s) 35
Hsp92II CATG 3 cut(s) 50, 65, 224
HspAI GCGC 2 cut(s) 39, 266
KspI CCGCGG 1 cut(s) 253
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 4 cut(s) 89, 157, 167, 287
MaeI CTAG 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 4 cut(s) 137, 149, 198, 320
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 1 cut(s) 352
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 6 cut(s) 16, 85, 125, 182, 218, 226
Mox20I TGGCCA 1 cut(s) 60
MscI TGGCCA 1 cut(s) 60
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 2 cut(s) 51, 288
Msp20I TGGCCA 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 1 cut(s) 154
MvnI CGCG 2 cut(s) 252, 268
NdeII GATC 1 cut(s) 114
NlaIII CATG 3 cut(s) 50, 65, 224
NspV TTCGAA 2 cut(s) 195, 331
PaqCI CACCTGC 2 cut(s) 162, 292
PfeI GAWTC 1 cut(s) 197
PinAI ACCGGT 1 cut(s) 153
PkrI GCNGC 1 cut(s) 251
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PshAI GACNNNNGTC 1 cut(s) 312
PspPI GGNCC 2 cut(s) 307, 314
PstI CTGCAG 1 cut(s) 173
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 2 cut(s) 51, 288
SacII CCGCGG 1 cut(s) 253
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 307, 314
SchI GAGTC 1 cut(s) 81
SduI GDGCHC 1 cut(s) 235
SetI ASST 4 cut(s) 70, 176, 306, 312
SfcI CTRYAG 2 cut(s) 169, 275
Sfr303I CCGCGG 1 cut(s) 253
SfuI TTCGAA 2 cut(s) 195, 331
SgrBI CCGCGG 1 cut(s) 253
SinI GGWCC 2 cut(s) 307, 314
SmiMI CAYNNNNRTG 2 cut(s) 51, 288
Sse9I AATT 1 cut(s) 352
SsiI CCGC 3 cut(s) 14, 250, 252
SspI AATATT 1 cut(s) 243
SspMI CTAG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 230, 287
TaqI TCGA 3 cut(s) 21, 195, 331
TasI AATT 1 cut(s) 352
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 217
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 307, 314
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.