Rh2BG299500

DNA repair metallo-beta-lactamase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015940
Physical Location & Seq
Forward (+)
34353108 .. 34356996
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG299500.1

Sequence Viewer

Length: 1452 bp
ATGTTCTTGTTTGAAGGCGATTTTGGTAATGTCCTCCACACCGGAGATTGCAGACTCACACCCGAATATCTGCAACGTTTACCAGACGAGTACCTTGGTAAGAAAGGGAAAAAGCCTTGGTGTCAACTCAATTATGTTTTTCTAGATTGCACGTTTGGAAAATACTATCAAAGTTTTCCCAGCAAACATTCAGCAATCCAGCAGGTCATTAATTGTATATGGAAGCATTCTGATGCAACTGAGGTGTATTTAGCCTGTGATCTCCTTGGTCAGGAAGAGATATTGGTTGATGTGTCCCGTACATTTGGATCAAAGATATATGTTGACAAAGTCTACAATCCTGAGTATTTTGATGCTCTGACAGTCATAGCTCCTGAAATCATATCCCAAGATCCGTCTTCATGCTTCCGGGTGCTTGATGCATTTCCAAAACTCAACGAGAGAGCCAAAGCAAAGCTTGCAGAGGCTCATGTTAACTTAAAGCCTGAGCCTCTCATAATCCGTCCTTCAGCACAGTGGTATGCTTGTGAGGTAGAATTGCTAGATAACGAAAGTCAAAGAAAACTGAGATTCAATGAAGCAGTAAGGGACCAATTTGGTGTTTGGCATGTTTGTTACTCAATGCACTCGTCCAGGGATGAATTAGAGTGGGCTCTGCAGCTTCTATTACCTAAATGGGTTGTTTCAACCACCCCCAGTTGCAGGGCTATGGAGCTGAATTATGTTAAGAAGCACTGTTTGACCTCTCGAATATCTCCAACTGATCCTCTTTGGAAACTTCTAGACATTAGTATGGAACCTTCTTCAGTTGCAGAAGTATCAATTGATTATGGGGGATCTCCAGTGTCTGAAGAGCTCAATCAAAGTCCTGCAGATTCTCAATTGCAACTGATTAGTAAATCTGCTTCCCCGAAAAAGTTCTTCAGTTTCTCTCCTCCTAGGAAAAGACCGTTTGTCACATTATTTGGTAGAGCTAGGTTTGGTTTCCAAGAATTGGCTATTCAGCACAAAGAGTCAAAAAATTTATACCTGAAAGATAAACCTTTTCAAGAAGTGGGCAACAGAGTTGGAGCAAATTTGTCTGGTGAGGGGGAAGAAAACAGTGAACACAGATGCTGCAGCAAGAATTTGGTGAAAAAGGTAGAGGAAAATGCTAATGAATTACAATGTGAAAAGCCAGGGGAGAGAAGGTCTGAACTGGAGCATGTACAACTGACTTCATGGGATGAACGTAATCAGAAGTATGAGCTGGAAAAGAAAAGACCAATTGAACCAGAAGCTCAAAATGTTTCTTGTTCTCTGATTGGGTCGTCAAAGTGCTTCAATGAAAGATTCAGAAAATTATACAGGTCAATGAATGTGGCTGTGCCTCAGCCGCTTCCTTCTCTAGTCGAACTTATGAATGCCCGCAAGCGTGCTAAGAGGAGAGTTGATTTCTCTTCTTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRMBL Lactamase_B_2

Protein Analysis

483

Amino Acids

55.56

Weight (kDa)

7.82

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 124 - 234 4.1e-18 DNA repair metallo-beta-lactamase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19025 AT1G19025 AT1G19025
fragaria_vesca FvH4_1g24320
pyrus_communis pycom01g02340
rosa_chinensis RchiOBHm_Chr2g0120831
rosa_laevigata RLG00000018593
rosa_multiflora Rmu_sc0000621.1_g000008
rosa_roxburghii Rroxscaffold_2G00122800
rosa_rugosa Rorug02G0231900
rosa_samantha Rh2AG288500 Rh2BG299500 Rh2CG277500 Rh2DG313600
rosa_wichuraiana Rw2G023110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 994
AccI GTMKAC 1 cut(s) 333
AciI CCGC 2 cut(s) 1376, 1408
AclI AACGTT 1 cut(s) 76
AclWI GGATC 4 cut(s) 316, 386, 758, 844
AcsI RAATTY 3 cut(s) 1021, 1075, 1126
AcuI CTGAAG 4 cut(s) 492, 789, 870, 907
AfaI GTAC 3 cut(s) 92, 301, 1209
AfiI CCNNNNNNNGG 3 cut(s) 271, 702, 994
AgsI TTSAA 6 cut(s) 14, 574, 687, 1049, 1271, 1324
AhdI GACNNNNNGTC 1 cut(s) 953
AjnI CCWGG 2 cut(s) 632, 1177
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 8 cut(s) 371, 457, 661, 715, 856, 974, 1249, 1280
AluI AGCT 8 cut(s) 371, 457, 661, 715, 856, 974, 1249, 1280
Alw21I GWGCWC 1 cut(s) 858
AlwI GGATC 4 cut(s) 316, 386, 758, 844
AlwNI CAGNNNCTG 2 cut(s) 848, 1116
ApeKI GCWGC 3 cut(s) 658, 1116, 1119
ApoI RAATTY 3 cut(s) 1021, 1075, 1126
AseI ATTAAT 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 917
AspA2I CCTAGG 1 cut(s) 938
AspS9I GGNCC 1 cut(s) 589
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 2 cut(s) 1097, 1144
AvaII GGWCC 1 cut(s) 589
AvrII CCTAGG 1 cut(s) 938
BanII GRGCYC 2 cut(s) 655, 858
BarI GAAGNNNNNNTAC 4 cut(s) 784, 816, 889, 921
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 858
BbvCI CCTCAGC 1 cut(s) 1371
BbvI GCAGC 3 cut(s) 670, 1103, 1131
BciT130I CCWGG 2 cut(s) 634, 1179
BcnI CCSGG 1 cut(s) 410
BfaI CTAG 6 cut(s) 143, 542, 782, 939, 975, 1388
BfmI CTRYAG 3 cut(s) 656, 870, 1117
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 4 cut(s) 659, 1117, 1120, 1376
BlnI CCTAGG 1 cut(s) 938
BlsI GCNGC 4 cut(s) 660, 1118, 1121, 1377
Bme1390I CCNGG 3 cut(s) 410, 634, 1179
Bme18I GGWCC 1 cut(s) 589
BmeRI GACNNNNNGTC 1 cut(s) 953
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 2 cut(s) 590, 798
BmrFI CCNGG 3 cut(s) 410, 634, 1179
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 4 cut(s) 223, 343, 409, 1103
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 2 cut(s) 825, 1220
Bpu10I CCTNAGC 2 cut(s) 486, 1371
BpuMI CCSGG 1 cut(s) 410
BsaJI CCNNGG 6 cut(s) 94, 116, 265, 633, 938, 1178
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 1175, 1205
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 702, 994
Bse1I ACTGG 3 cut(s) 696, 842, 1203
BseBI CCWGG 2 cut(s) 634, 1179
BseDI CCNNGG 6 cut(s) 94, 116, 265, 633, 938, 1178
BseGI GGATG 2 cut(s) 643, 1231
BseLI CCNNNNNNNGG 3 cut(s) 271, 702, 994
BseMII CTCAG 5 cut(s) 231, 333, 477, 557, 1385
BseNI ACTGG 3 cut(s) 696, 842, 1203
BseRI GAGGAG 2 cut(s) 924, 1438
BseXI GCAGC 3 cut(s) 670, 1103, 1131
BseYI CCCAGC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 858
BsiSI CCGG 2 cut(s) 42, 409
BslFI GGGAC 2 cut(s) 280, 602
BslI CCNNNNNNNGG 3 cut(s) 271, 702, 994
BsmFI GGGAC 2 cut(s) 280, 602
BsmI GAATGC 2 cut(s) 226, 1408
Bsp1286I GDGCHC 2 cut(s) 655, 858
Bsp1407I TGTACA 1 cut(s) 1207
Bsp143I GATC 5 cut(s) 259, 308, 391, 763, 836
BspACI CCGC 2 cut(s) 1376, 1408
BspCNI CTCAG 5 cut(s) 232, 334, 478, 558, 1384
BspLI GGNNCC 2 cut(s) 590, 798
BspMAI CTGCAG 3 cut(s) 660, 874, 1121
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 4 cut(s) 316, 386, 758, 844
BspQI GCTCTTC 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 3 cut(s) 696, 842, 1203
BssECI CCNNGG 6 cut(s) 94, 116, 265, 633, 938, 1178
BssMI GATC 5 cut(s) 259, 308, 391, 763, 836
BssT1I CCWWGG 4 cut(s) 94, 116, 265, 938
Bst2UI CCWGG 2 cut(s) 634, 1179
Bst4CI ACNGT 5 cut(s) 364, 516, 737, 951, 1103
Bst6I CTCTTC 3 cut(s) 270, 846, 1444
BstAPI GCANNNNNTGC 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 1207
BstC8I GCNNGC 4 cut(s) 459, 1408, 1412, 1416
BstDEI CTNAG 6 cut(s) 240, 342, 486, 566, 1371, 1419
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 643, 1231
BstKTI GATC 5 cut(s) 262, 311, 394, 766, 839
BstMBI GATC 5 cut(s) 259, 308, 391, 763, 836
BstMWI GCNNNNNNNGC 2 cut(s) 458, 1375
BstNI CCWGG 2 cut(s) 634, 1179
BstNSI RCATGY 2 cut(s) 611, 1208
BstSCI CCNGG 3 cut(s) 408, 632, 1177
BstSFI CTRYAG 3 cut(s) 656, 870, 1117
BstV1I GCAGC 3 cut(s) 670, 1103, 1131
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 391, 836
BstYI RGATCY 2 cut(s) 391, 836
BtsCI GGATG 2 cut(s) 643, 1231
BtsIMutI CAGTG 4 cut(s) 521, 733, 849, 1108
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 4 cut(s) 459, 1408, 1412, 1416
CaiI CAGNNNCTG 2 cut(s) 848, 1116
Cfr13I GGNCC 1 cut(s) 589
Csp6I GTAC 3 cut(s) 91, 300, 1208
CspCI CAANNNNNGTGG 2 cut(s) 1341, 1376
CviAII CATG 5 cut(s) 402, 470, 608, 1205, 1221
CviQI GTAC 3 cut(s) 91, 300, 1208
DdeI CTNAG 6 cut(s) 240, 342, 486, 566, 1371, 1419
DpnI GATC 5 cut(s) 261, 310, 393, 765, 838
DpnII GATC 5 cut(s) 259, 308, 391, 763, 836
DriI GACNNNNNGTC 1 cut(s) 953
Eam1104I CTCTTC 3 cut(s) 270, 846, 1444
Eam1105I GACNNNNNGTC 1 cut(s) 953
EarI CTCTTC 3 cut(s) 270, 846, 1444
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 4 cut(s) 94, 116, 265, 938
Eco24I GRGCYC 2 cut(s) 655, 858
Eco47I GGWCC 1 cut(s) 589
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 4 cut(s) 492, 789, 870, 907
EcoICRI GAGCTC 1 cut(s) 856
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoRII CCWGG 2 cut(s) 632, 1177
EcoT14I CCWWGG 4 cut(s) 94, 116, 265, 938
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 655, 858
ErhI CCWWGG 4 cut(s) 94, 116, 265, 938
FaeI CATG 5 cut(s) 405, 473, 611, 1208, 1224
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 2 cut(s) 280, 602
FatI CATG 5 cut(s) 401, 469, 607, 1204, 1220
FauI CCCGC 1 cut(s) 1415
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 4 cut(s) 659, 1117, 1120, 1376
FokI GGATG 2 cut(s) 650, 1238
FriOI GRGCYC 2 cut(s) 655, 858
Fsp4HI GCNGC 4 cut(s) 659, 1117, 1120, 1376
FspBI CTAG 6 cut(s) 143, 542, 782, 939, 975, 1388
GluI GCNGC 4 cut(s) 659, 1117, 1120, 1376
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 2 cut(s) 825, 1220
HapII CCGG 2 cut(s) 42, 409
Hin1II CATG 5 cut(s) 405, 473, 611, 1208, 1224
HincII GTYRAC 3 cut(s) 125, 325, 475
HindII GTYRAC 3 cut(s) 125, 325, 475
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 54, 570, 875, 1013, 1332
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 2 cut(s) 42, 409
HphI GGTGA 2 cut(s) 1097, 1144
Hpy166II GTNNAC 6 cut(s) 80, 125, 325, 334, 475, 1106
Hpy188I TCNGA 7 cut(s) 232, 360, 850, 1195, 1239, 1302, 1337
Hpy188III TCNNGA 7 cut(s) 143, 272, 341, 374, 747, 782, 1049
Hpy8I GTNNAC 6 cut(s) 80, 125, 325, 334, 475, 1106
HpyAV CCTTC 5 cut(s) 8, 516, 810, 1182, 1392
HpyCH4III ACNGT 5 cut(s) 364, 516, 737, 951, 1103
HpyCH4IV ACGT 3 cut(s) 76, 152, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 1375
HpyF3I CTNAG 6 cut(s) 240, 342, 486, 566, 1371, 1419
HpySE526I ACGT 3 cut(s) 76, 152, 1231
Hsp92II CATG 5 cut(s) 405, 473, 611, 1208, 1224
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 5 cut(s) 259, 308, 391, 763, 836
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 4 cut(s) 376, 712, 1070, 1201
Lsp1109I GCAGC 3 cut(s) 670, 1103, 1131
LweI GCATC 4 cut(s) 223, 343, 409, 1103
MaeI CTAG 6 cut(s) 143, 542, 782, 939, 975, 1388
MaeII ACGT 3 cut(s) 76, 152, 1231
MaeIII GTNAC 2 cut(s) 614, 955
MalI GATC 5 cut(s) 261, 310, 393, 765, 838
MboI GATC 5 cut(s) 259, 308, 391, 763, 836
MboII GAAGA 7 cut(s) 287, 390, 795, 863, 913, 1106, 1431
MfeI CAATTG 3 cut(s) 822, 881, 1266
MflI RGATCY 2 cut(s) 391, 836
MhlI GDGCHC 2 cut(s) 655, 858
MlyI GAGTC 2 cut(s) 48, 1022
MmeI TCCRAC 2 cut(s) 782, 1048
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 6 cut(s) 210, 474, 479, 726, 1446, 1450
MslI CAYNNNNRTG 2 cut(s) 231, 791
MspI CCGG 2 cut(s) 42, 409
MspR9I CCNGG 3 cut(s) 410, 634, 1179
MunI CAATTG 3 cut(s) 822, 881, 1266
Mva1269I GAATGC 2 cut(s) 226, 1408
MvaI CCWGG 2 cut(s) 634, 1179
MwoI GCNNNNNNNGC 2 cut(s) 458, 1375
NciI CCSGG 1 cut(s) 410
NdeII GATC 5 cut(s) 259, 308, 391, 763, 836
NlaIII CATG 5 cut(s) 405, 473, 611, 1208, 1224
NlaIV GGNNCC 2 cut(s) 590, 798
NmuCI GTSAC 1 cut(s) 955
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 611, 1208
PacI TTAATTAA 1 cut(s) 1450
PciSI GCTCTTC 1 cut(s) 846
PctI GAATGC 2 cut(s) 226, 1408
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 3 cut(s) 570, 875, 1332
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 994
PkrI GCNGC 4 cut(s) 660, 1118, 1121, 1377
PleI GAGTC 2 cut(s) 48, 1021
PpsI GAGTC 2 cut(s) 48, 1021
PshBI ATTAAT 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 858
Psp1406I AACGTT 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 632, 1177
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 2 cut(s) 632, 1177
PspN4I GGNNCC 2 cut(s) 590, 798
PspPI GGNCC 1 cut(s) 589
PstI CTGCAG 3 cut(s) 660, 874, 1121
PstNI CAGNNNCTG 2 cut(s) 848, 1116
PsuI RGATCY 2 cut(s) 391, 836
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 3 cut(s) 92, 301, 1209
RsaNI GTAC 3 cut(s) 91, 300, 1208
RseI CAYNNNNRTG 2 cut(s) 231, 791
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 6 cut(s) 210, 474, 479, 726, 1446, 1450
SatI GCNGC 4 cut(s) 659, 1117, 1120, 1376
Sau3AI GATC 5 cut(s) 259, 308, 391, 763, 836
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 2 cut(s) 48, 1022
ScrFI CCNGG 3 cut(s) 410, 634, 1179
SduI GDGCHC 2 cut(s) 655, 858
SfaNI GCATC 4 cut(s) 223, 343, 409, 1103
SfcI CTRYAG 3 cut(s) 656, 870, 1117
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 231, 791
SsiI CCGC 2 cut(s) 1376, 1408
SspMI CTAG 6 cut(s) 143, 542, 782, 939, 975, 1388
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 3 cut(s) 408, 632, 1177
StyI CCWWGG 4 cut(s) 94, 116, 265, 938
TaaI ACNGT 5 cut(s) 364, 516, 737, 951, 1103
TaiI ACGT 3 cut(s) 79, 155, 1234
TaqI TCGA 2 cut(s) 748, 1392
TatI WGTACW 1 cut(s) 1207
TauI GCSGC 1 cut(s) 1378
TfiI GAWTC 3 cut(s) 570, 875, 1332
Tru1I TTAA 6 cut(s) 210, 474, 479, 726, 1446, 1450
Tru9I TTAA 6 cut(s) 210, 474, 479, 726, 1446, 1450
TscAI CASTG 4 cut(s) 521, 740, 849, 1108
TseFI GTSAC 1 cut(s) 955
TseI GCWGC 3 cut(s) 658, 1116, 1119
Tsp45I GTSAC 1 cut(s) 955
TspDTI ATGAA 9 cut(s) 390, 591, 654, 1173, 1209, 1242, 1341, 1370, 1415
TspGWI ACGGA 2 cut(s) 384, 491
TspRI CASTG 4 cut(s) 521, 740, 849, 1108
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 994
VpaK11BI GGWCC 1 cut(s) 589
VspI ATTAAT 1 cut(s) 210
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 3 cut(s) 1021, 1075, 1126
XbaI TCTAGA 2 cut(s) 142, 781
XceI RCATGY 2 cut(s) 611, 1208
XmaJI CCTAGG 1 cut(s) 938
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 6 cut(s) 143, 542, 782, 939, 975, 1388
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.