Rw2G023110

DNA repair metallo-beta-lactamase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015940
Physical Location & Seq
Forward (+)
33395096 .. 33398896
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023110.1

Sequence Viewer

Length: 1335 bp
ATGTCGATCGAAATGCCCAAAGGGCTCCCCTTTTCAGTTGATACATGGAGCCCTTGTTCAAAGAGAAAGAGGCACCATTTCTTAACCCACGCCCACAAAGACCACTCCATTGGCATCTCCTCCCACTTTTCTTACCCAATTTACTCCACCCATCTCACCAAAACCCTCCTTCTTCAACACTACCCCAAGCTTGATGAATCTTTGTTTCTGGGCATTGAGGTTGGCCAATCTGTAGTCATTGATGACCCGGATGGAGTCTTCACTGTTACGGCTTTCGATGCCAATCACTGTCCTGGAGCTGTCATGTTCTTGTTTGAAGGCGATTTTGGTAATGTCCTCCACACCGGAGATTGCAGACTCACACCCGAATATCTGCAGCGTTTACCAGACAAGTACCTTGGTAAGAAAGGGAAAAAGCCTAGGTGTCAACTCAATTATGTTTTTCTAGATTGCACGTTTGGAAAATACTATCAAAGTTTTCCCAGCAAACATTCAGCAATCCAGCAGGTCATTAATTGTATATGGAAGCATCCTGATGCAACTGAGGTGTATTTAGCCTGTGATCTCCTCGGTCAGGAAGAGATATTGGTTGATGTGTCCCGTTCATTTGGATCAAAGATATATGTTGACAAAGTCTCCAATCCTGAGTATTTTGATGCTCTGACAGTCATAGCTCCTGAAATCATATCCCAAGATCCGTCTTCACGCTTCCAGGTGCTTGATGCATTTCCAAAACTGAACGAGAGAGCCAAAGCAAAGCTTGCAGAGGCCCATGTTAACTTAAAGCCTGAGCCTCTCATAATCCGTCCTTCAGCACAGTGGTATGCTTGTGAGGTAGAATTGCTAGATAATGACAGTCAAAGAAAACTGAGATTCAATGAAGCAGTAAGGGACCAATTTGGTGTTTGGCATGTTTGTTACTCAATGCACTCGTCCATGGATGAATTAGAGTGGGCTCTGCAGCTTCTATTACCTAAATGGGTTGTTTCAACCACCCCCAGTTGCAGGGCTATGGAGCTGAATTATGTTAAGAAGCACTGTTTGACCTCTCGAATATCTCCAACTGATCCTCTTTGGAAACTTCTAGACATTAGTATGGAACCTTCTTCAGTTGCAGAAGTATCAATTGATTATGGGGGATCTCCAGTGTCTGAAGAGCTCAATCAAAGTCCTGCAGATTCTCAATTGCAACTGATAAGTAAATCTGCTTCCCCGAAAAAGTTCTTTAGTTTCTCTCCTCCTAGGAAAAGACCGTTTGTCACATTATTTGGTAGAGCTAGGTTTGGTTTCCAAGAATTGGCTATTCAGCACAAAGAGGCAAAATTTTATACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRMBL Lactamase_B_2

Protein Analysis

444

Amino Acids

50.52

Weight (kDa)

6.78

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 225 - 335 1.7e-18 DNA repair metallo-beta-lactamase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19025 AT1G19025 AT1G19025
fragaria_vesca FvH4_1g24320
pyrus_communis pycom01g02340
rosa_chinensis RchiOBHm_Chr2g0120831
rosa_laevigata RLG00000018593
rosa_multiflora Rmu_sc0000621.1_g000008
rosa_roxburghii Rroxscaffold_2G00122800
rosa_rugosa Rorug02G0231900
rosa_samantha Rh2AG288500 Rh2BG299500 Rh2CG277500 Rh2DG313600
rosa_wichuraiana Rw2G023110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 496
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 1297
AclWI GGATC 4 cut(s) 619, 689, 1061, 1147
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 1322
AcuI CTGAAG 3 cut(s) 795, 1092, 1173
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 3 cut(s) 574, 1005, 1297
AgsI TTSAA 5 cut(s) 60, 176, 317, 877, 990
AhdI GACNNNNNGTC 1 cut(s) 1256
AjnI CCWGG 2 cut(s) 292, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AloI GAACNNNNNNTCC 2 cut(s) 40, 72
AluBI AGCT 8 cut(s) 190, 299, 674, 760, 964, 1018, 1159, 1277
AluI AGCT 8 cut(s) 190, 299, 674, 760, 964, 1018, 1159, 1277
Alw21I GWGCWC 1 cut(s) 1161
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 4 cut(s) 619, 689, 1061, 1147
AlwNI CAGNNNCTG 1 cut(s) 1151
AoxI GGCC 2 cut(s) 223, 768
ApeKI GCWGC 2 cut(s) 376, 961
ApoI RAATTY 1 cut(s) 1322
AseI ATTAAT 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 1220
AspA2I CCTAGG 2 cut(s) 419, 1241
AspS9I GGNCC 2 cut(s) 769, 892
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 892
AvrII CCTAGG 2 cut(s) 419, 1241
BalI TGGCCA 1 cut(s) 225
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 4 cut(s) 27, 53, 958, 1161
BarI GAAGNNNNNNTAC 4 cut(s) 1087, 1119, 1192, 1224
BbsI GAAGAC 2 cut(s) 250, 693
Bbv12I GWGCWC 1 cut(s) 1161
BbvI GCAGC 2 cut(s) 388, 973
BccI CCATC 2 cut(s) 159, 245
BceAI ACGGC 1 cut(s) 285
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 294, 713
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 6 cut(s) 420, 446, 845, 1085, 1242, 1278
BfmI CTRYAG 4 cut(s) 231, 374, 959, 1173
BfuAI ACCTGC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 22
BisI GCNGC 2 cut(s) 377, 962
BlnI CCTAGG 2 cut(s) 419, 1241
BlsI GCNGC 2 cut(s) 378, 963
Bme1390I CCNGG 3 cut(s) 248, 294, 713
Bme18I GGWCC 1 cut(s) 892
BmeRI GACNNNNNGTC 1 cut(s) 1256
BmgT120I GGNCC 2 cut(s) 769, 892
BmiI GGNNCC 5 cut(s) 26, 50, 74, 893, 1101
BmrFI CCNGG 3 cut(s) 248, 294, 713
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 6 cut(s) 123, 268, 526, 538, 646, 712
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 2 cut(s) 250, 693
BpmI CTGGAG 2 cut(s) 315, 1128
Bpu10I CCTNAGC 1 cut(s) 789
BpuMI CCSGG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 5 cut(s) 397, 419, 568, 936, 1241
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 620, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 1005, 1297
Bse1I ACTGG 2 cut(s) 999, 1145
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 2 cut(s) 294, 713
BseDI CCNNGG 5 cut(s) 397, 419, 568, 936, 1241
BseGI GGATG 3 cut(s) 256, 529, 946
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 574, 1005, 1297
BseMII CTCAG 4 cut(s) 534, 636, 780, 860
BseNI ACTGG 2 cut(s) 999, 1145
BseRI GAGGAG 3 cut(s) 109, 557, 1227
BseXI GCAGC 2 cut(s) 388, 973
BseYI CCCAGC 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 225, 770
BshNI GGYRCC 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 1161
BsiSI CCGG 2 cut(s) 248, 345
BslFI GGGAC 2 cut(s) 583, 905
BslI CCNNNNNNNGG 3 cut(s) 574, 1005, 1297
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 2 cut(s) 583, 905
BsnI GGCC 2 cut(s) 225, 770
Bsp1286I GDGCHC 4 cut(s) 27, 53, 958, 1161
Bsp143I GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
Bsp19I CCATGG 1 cut(s) 936
BspANI GGCC 2 cut(s) 225, 770
BspCNI CTCAG 4 cut(s) 535, 637, 781, 861
BspLI GGNNCC 5 cut(s) 26, 50, 74, 893, 1101
BspMAI CTGCAG 3 cut(s) 378, 963, 1177
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 4 cut(s) 619, 689, 1061, 1147
BspQI GCTCTTC 1 cut(s) 1149
BspT107I GGYRCC 1 cut(s) 72
BsrI ACTGG 2 cut(s) 999, 1145
BssECI CCNNGG 5 cut(s) 397, 419, 568, 936, 1241
BssMI GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
BssT1I CCWWGG 4 cut(s) 397, 419, 936, 1241
Bst2UI CCWGG 2 cut(s) 294, 713
Bst4CI ACNGT 7 cut(s) 265, 290, 667, 819, 857, 1040, 1254
Bst6I CTCTTC 2 cut(s) 573, 1149
BstAPI GCANNNNNTGC 1 cut(s) 761
BstC8I GCNNGC 1 cut(s) 762
BstDEI CTNAG 4 cut(s) 543, 645, 789, 869
BstDSI CCRYGG 1 cut(s) 936
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 3 cut(s) 256, 529, 946
BstKTI GATC 6 cut(s) 9, 565, 614, 697, 1069, 1142
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 22, 278, 761
BstNI CCWGG 2 cut(s) 294, 713
BstNSI RCATGY 1 cut(s) 914
BstSCI CCNGG 3 cut(s) 246, 292, 711
BstSFI CTRYAG 4 cut(s) 231, 374, 959, 1173
BstV1I GCAGC 2 cut(s) 388, 973
BstV2I GAAGAC 2 cut(s) 250, 693
BstX2I RGATCY 2 cut(s) 694, 1139
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 2 cut(s) 694, 1139
BsuRI GGCC 2 cut(s) 225, 770
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 3 cut(s) 256, 529, 946
BtsIMutI CAGTG 5 cut(s) 261, 286, 824, 1036, 1152
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 762
CaiI CAGNNNCTG 1 cut(s) 1151
Cfr13I GGNCC 2 cut(s) 769, 892
Csp6I GTAC 1 cut(s) 394
CviAII CATG 5 cut(s) 45, 304, 773, 911, 937
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 4 cut(s) 543, 645, 789, 869
DpnI GATC 6 cut(s) 8, 564, 613, 696, 1068, 1141
DpnII GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
DriI GACNNNNNGTC 1 cut(s) 1256
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 573, 1149
Eam1105I GACNNNNNGTC 1 cut(s) 1256
EarI CTCTTC 2 cut(s) 573, 1149
Ecl136II GAGCTC 1 cut(s) 1159
Eco130I CCWWGG 4 cut(s) 397, 419, 936, 1241
Eco24I GRGCYC 4 cut(s) 27, 53, 958, 1161
Eco47I GGWCC 1 cut(s) 892
Eco53kI GAGCTC 1 cut(s) 1159
Eco57I CTGAAG 3 cut(s) 795, 1092, 1173
EcoICRI GAGCTC 1 cut(s) 1159
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 292, 711
EcoT14I CCWWGG 4 cut(s) 397, 419, 936, 1241
EcoT22I ATGCAT 1 cut(s) 727
EcoT38I GRGCYC 4 cut(s) 27, 53, 958, 1161
ErhI CCWWGG 4 cut(s) 397, 419, 936, 1241
FaeI CATG 5 cut(s) 48, 307, 776, 914, 940
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 2 cut(s) 583, 905
FatI CATG 5 cut(s) 44, 303, 772, 910, 936
Fnu4HI GCNGC 2 cut(s) 377, 962
FokI GGATG 3 cut(s) 263, 516, 953
FriOI GRGCYC 4 cut(s) 27, 53, 958, 1161
Fsp4HI GCNGC 2 cut(s) 377, 962
FspBI CTAG 6 cut(s) 420, 446, 845, 1085, 1242, 1278
GluI GCNGC 2 cut(s) 377, 962
GsaI CCCAGC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 315, 1128
HaeIII GGCC 2 cut(s) 225, 770
HapII CCGG 2 cut(s) 248, 345
Hin1II CATG 5 cut(s) 48, 307, 776, 914, 940
HincII GTYRAC 3 cut(s) 428, 628, 778
HindII GTYRAC 3 cut(s) 428, 628, 778
HindIII AAGCTT 2 cut(s) 188, 758
HinfI GANTC 5 cut(s) 197, 255, 357, 873, 1178
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 2 cut(s) 248, 345
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 4 cut(s) 383, 428, 628, 778
Hpy188I TCNGA 2 cut(s) 663, 1153
Hpy188III TCNNGA 7 cut(s) 446, 533, 575, 644, 677, 1050, 1085
Hpy8I GTNNAC 4 cut(s) 383, 428, 628, 778
HpyAV CCTTC 4 cut(s) 179, 311, 819, 1113
HpyCH4III ACNGT 7 cut(s) 265, 290, 667, 819, 857, 1040, 1254
HpyCH4IV ACGT 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 278, 761
HpyF3I CTNAG 4 cut(s) 543, 645, 789, 869
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 5 cut(s) 48, 307, 776, 914, 940
KspAI GTTAAC 1 cut(s) 778
Kzo9I GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
LguI GCTCTTC 1 cut(s) 1149
LmnI GCTCC 5 cut(s) 30, 48, 296, 679, 1015
Lsp1109I GCAGC 2 cut(s) 388, 973
LweI GCATC 6 cut(s) 123, 268, 526, 538, 646, 712
MaeI CTAG 6 cut(s) 420, 446, 845, 1085, 1242, 1278
MaeII ACGT 1 cut(s) 455
MaeIII GTNAC 3 cut(s) 265, 917, 1258
MalI GATC 6 cut(s) 8, 564, 613, 696, 1068, 1141
MboI GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
MboII GAAGA 6 cut(s) 164, 250, 590, 693, 1098, 1166
MfeI CAATTG 2 cut(s) 1125, 1184
MflI RGATCY 2 cut(s) 694, 1139
MhlI GDGCHC 4 cut(s) 27, 53, 958, 1161
MlsI TGGCCA 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 225
MlyI GAGTC 2 cut(s) 264, 351
MmeI TCCRAC 1 cut(s) 1085
Mox20I TGGCCA 1 cut(s) 225
Mph1103I ATGCAT 1 cut(s) 727
MroXI GAANNNNTTC 1 cut(s) 1220
MscI TGGCCA 1 cut(s) 225
MseI TTAA 5 cut(s) 83, 513, 777, 782, 1029
MslI CAYNNNNRTG 2 cut(s) 534, 1094
Msp20I TGGCCA 1 cut(s) 225
MspI CCGG 2 cut(s) 248, 345
MspR9I CCNGG 3 cut(s) 248, 294, 713
MunI CAATTG 2 cut(s) 1125, 1184
MvaI CCWGG 2 cut(s) 294, 713
MwoI GCNNNNNNNGC 3 cut(s) 22, 278, 761
NciI CCSGG 1 cut(s) 248
NcoI CCATGG 1 cut(s) 936
NdeII GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
NlaIII CATG 5 cut(s) 48, 307, 776, 914, 940
NlaIV GGNNCC 5 cut(s) 26, 50, 74, 893, 1101
NmuCI GTSAC 1 cut(s) 1258
NsiI ATGCAT 1 cut(s) 727
NspI RCATGY 1 cut(s) 914
PciSI GCTCTTC 1 cut(s) 1149
PdmI GAANNNNTTC 1 cut(s) 1220
PfeI GAWTC 3 cut(s) 197, 873, 1178
PflFI GACNNNGTC 1 cut(s) 632
PflMI CCANNNNNTGG 1 cut(s) 1297
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 2 cut(s) 378, 963
Ple19I CGATCG 1 cut(s) 9
PleI GAGTC 2 cut(s) 263, 351
PpsI GAGTC 2 cut(s) 263, 351
PshBI ATTAAT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 1161
Psp6I CCWGG 2 cut(s) 292, 711
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 2 cut(s) 292, 711
PspN4I GGNNCC 5 cut(s) 26, 50, 74, 893, 1101
PspPI GGNCC 2 cut(s) 769, 892
PstI CTGCAG 3 cut(s) 378, 963, 1177
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 2 cut(s) 694, 1139
PsyI GACNNNGTC 1 cut(s) 632
PvuI CGATCG 1 cut(s) 9
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 2 cut(s) 534, 1094
SacI GAGCTC 1 cut(s) 1161
SapI GCTCTTC 1 cut(s) 1149
SaqAI TTAA 5 cut(s) 83, 513, 777, 782, 1029
SatI GCNGC 2 cut(s) 377, 962
Sau3AI GATC 6 cut(s) 6, 562, 611, 694, 1066, 1139
Sau96I GGNCC 2 cut(s) 769, 892
SchI GAGTC 2 cut(s) 264, 351
ScrFI CCNGG 3 cut(s) 248, 294, 713
SduI GDGCHC 4 cut(s) 27, 53, 958, 1161
SfaNI GCATC 6 cut(s) 123, 268, 526, 538, 646, 712
SfcI CTRYAG 4 cut(s) 231, 374, 959, 1173
SinI GGWCC 1 cut(s) 892
SmiMI CAYNNNNRTG 2 cut(s) 534, 1094
SspMI CTAG 6 cut(s) 420, 446, 845, 1085, 1242, 1278
SstI GAGCTC 1 cut(s) 1161
StyD4I CCNGG 3 cut(s) 246, 292, 711
StyI CCWWGG 4 cut(s) 397, 419, 936, 1241
TaaI ACNGT 7 cut(s) 265, 290, 667, 819, 857, 1040, 1254
TaiI ACGT 1 cut(s) 458
TaqI TCGA 4 cut(s) 5, 9, 276, 1051
TaqII GACCGA 1 cut(s) 560
TfiI GAWTC 3 cut(s) 197, 873, 1178
Tru1I TTAA 5 cut(s) 83, 513, 777, 782, 1029
Tru9I TTAA 5 cut(s) 83, 513, 777, 782, 1029
TscAI CASTG 5 cut(s) 268, 293, 824, 1043, 1152
TseFI GTSAC 1 cut(s) 1258
TseI GCWGC 2 cut(s) 376, 961
Tsp45I GTSAC 1 cut(s) 1258
TspDTI ATGAA 4 cut(s) 210, 594, 894, 957
TspGWI ACGGA 2 cut(s) 687, 794
TspRI CASTG 5 cut(s) 268, 293, 824, 1043, 1152
Tth111I GACNNNGTC 1 cut(s) 632
Van91I CCANNNNNTGG 1 cut(s) 1297
VpaK11BI GGWCC 1 cut(s) 892
VspI ATTAAT 1 cut(s) 513
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 1 cut(s) 1322
XbaI TCTAGA 2 cut(s) 445, 1084
XceI RCATGY 1 cut(s) 914
XmaJI CCTAGG 2 cut(s) 419, 1241
XmnI GAANNNNTTC 1 cut(s) 1220
XspI CTAG 6 cut(s) 420, 446, 845, 1085, 1242, 1278
Zsp2I ATGCAT 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.