Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr2g0166421 |
| rosa_multiflora | Rmu_sc0003016.1_g000008 |
| rosa_rugosa | Rorug02G0522800 |
| rosa_samantha | Rh2BG601000 Rh2DG611200 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 210 |
| AciI | CCGC | 2 cut(s) | 287, 614 |
| AclWI | GGATC | 3 cut(s) | 573, 717, 730 |
| AcsI | RAATTY | 3 cut(s) | 699, 849, 929 |
| AcuI | CTGAAG | 2 cut(s) | 236, 545 |
| AcvI | CACGTG | 1 cut(s) | 235 |
| AfaI | GTAC | 5 cut(s) | 107, 321, 334, 467, 719 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 410 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 915, 947 |
| AluBI | AGCT | 4 cut(s) | 100, 360, 533, 559 |
| AluI | AGCT | 4 cut(s) | 100, 360, 533, 559 |
| Alw21I | GWGCWC | 1 cut(s) | 362 |
| Alw26I | GTCTC | 2 cut(s) | 759, 903 |
| AlwI | GGATC | 3 cut(s) | 573, 717, 730 |
| AoxI | GGCC | 1 cut(s) | 735 |
| ApeKI | GCWGC | 2 cut(s) | 64, 594 |
| ApoI | RAATTY | 3 cut(s) | 699, 849, 929 |
| AspS9I | GGNCC | 1 cut(s) | 667 |
| AsuHPI | GGTGA | 2 cut(s) | 780, 848 |
| AvaII | GGWCC | 1 cut(s) | 667 |
| BamHI | GGATCC | 1 cut(s) | 722 |
| BanII | GRGCYC | 1 cut(s) | 362 |
| BbrPI | CACGTG | 1 cut(s) | 235 |
| Bbv12I | GWGCWC | 1 cut(s) | 362 |
| BbvI | GCAGC | 2 cut(s) | 76, 581 |
| BccI | CCATC | 3 cut(s) | 240, 401, 631 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 134, 168 |
| BcoDI | GTCTC | 2 cut(s) | 759, 903 |
| BfaI | CTAG | 1 cut(s) | 747 |
| BfmI | CTRYAG | 2 cut(s) | 62, 595 |
| BisI | GCNGC | 3 cut(s) | 65, 595, 615 |
| BlsI | GCNGC | 3 cut(s) | 66, 596, 616 |
| Bme18I | GGWCC | 1 cut(s) | 667 |
| BmgT120I | GGNCC | 1 cut(s) | 667 |
| BmiI | GGNNCC | 1 cut(s) | 724 |
| BmsI | GCATC | 2 cut(s) | 274, 588 |
| BpmI | CTGGAG | 1 cut(s) | 122 |
| BpuEI | CTTGAG | 2 cut(s) | 367, 777 |
| BsaAI | YACGTR | 2 cut(s) | 109, 235 |
| BsaBI | GATNNNNATC | 1 cut(s) | 681 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 410 |
| Bse118I | RCCGGY | 1 cut(s) | 590 |
| Bse1I | ACTGG | 1 cut(s) | 574 |
| Bse8I | GATNNNNATC | 1 cut(s) | 681 |
| BseGI | GGATG | 3 cut(s) | 289, 493, 1131 |
| BseJI | GATNNNNATC | 1 cut(s) | 681 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 410 |
| BseMII | CTCAG | 1 cut(s) | 746 |
| BseNI | ACTGG | 1 cut(s) | 574 |
| BseRI | GAGGAG | 1 cut(s) | 826 |
| BseXI | GCAGC | 2 cut(s) | 76, 581 |
| BsgI | GTGCAG | 1 cut(s) | 995 |
| BshFI | GGCC | 1 cut(s) | 737 |
| BsiHKAI | GWGCWC | 1 cut(s) | 362 |
| BsiSI | CCGG | 1 cut(s) | 591 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 410 |
| BsmAI | GTCTC | 2 cut(s) | 759, 903 |
| BsnI | GGCC | 1 cut(s) | 737 |
| Bsp1286I | GDGCHC | 1 cut(s) | 362 |
| Bsp143I | GATC | 2 cut(s) | 565, 722 |
| BspACI | CCGC | 2 cut(s) | 287, 614 |
| BspANI | GGCC | 1 cut(s) | 737 |
| BspCNI | CTCAG | 1 cut(s) | 747 |
| BspHI | TCATGA | 1 cut(s) | 585 |
| BspLI | GGNNCC | 1 cut(s) | 724 |
| BspMAI | CTGCAG | 2 cut(s) | 66, 599 |
| BspPI | GGATC | 3 cut(s) | 573, 717, 730 |
| BsrFI | RCCGGY | 1 cut(s) | 590 |
| BsrI | ACTGG | 1 cut(s) | 574 |
| BssAI | RCCGGY | 1 cut(s) | 590 |
| BssMI | GATC | 2 cut(s) | 565, 722 |
| Bst4CI | ACNGT | 1 cut(s) | 845 |
| BstBAI | YACGTR | 2 cut(s) | 109, 235 |
| BstC8I | GCNNGC | 1 cut(s) | 38 |
| BstDEI | CTNAG | 1 cut(s) | 755 |
| BstF5I | GGATG | 3 cut(s) | 289, 493, 1131 |
| BstKTI | GATC | 2 cut(s) | 568, 725 |
| BstMAI | GTCTC | 2 cut(s) | 759, 903 |
| BstMBI | GATC | 2 cut(s) | 565, 722 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 169 |
| BstNSI | RCATGY | 3 cut(s) | 172, 232, 318 |
| BstSFI | CTRYAG | 2 cut(s) | 62, 595 |
| BstV1I | GCAGC | 2 cut(s) | 76, 581 |
| BstX2I | RGATCY | 1 cut(s) | 722 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 733 |
| BstYI | RGATCY | 1 cut(s) | 722 |
| BsuRI | GGCC | 1 cut(s) | 737 |
| BtgZI | GCGATG | 1 cut(s) | 259 |
| BtsCI | GGATG | 3 cut(s) | 289, 493, 1131 |
| BtsIMutI | CAGTG | 1 cut(s) | 581 |
| Cac8I | GCNNGC | 1 cut(s) | 38 |
| CciI | TCATGA | 1 cut(s) | 585 |
| Cfr10I | RCCGGY | 1 cut(s) | 590 |
| Cfr13I | GGNCC | 1 cut(s) | 667 |
| Csp6I | GTAC | 5 cut(s) | 106, 320, 333, 466, 718 |
| CviAII | CATG | 7 cut(s) | 169, 229, 315, 586, 689, 733, 937 |
| CviQI | GTAC | 5 cut(s) | 106, 320, 333, 466, 718 |
| DdeI | CTNAG | 1 cut(s) | 755 |
| DpnI | GATC | 2 cut(s) | 567, 724 |
| DpnII | GATC | 2 cut(s) | 565, 722 |
| Ecl136II | GAGCTC | 1 cut(s) | 360 |
| Eco24I | GRGCYC | 1 cut(s) | 362 |
| Eco47I | GGWCC | 1 cut(s) | 667 |
| Eco53kI | GAGCTC | 1 cut(s) | 360 |
| Eco57I | CTGAAG | 2 cut(s) | 236, 545 |
| Eco72I | CACGTG | 1 cut(s) | 235 |
| EcoICRI | GAGCTC | 1 cut(s) | 360 |
| EcoRI | GAATTC | 1 cut(s) | 849 |
| EcoT22I | ATGCAT | 1 cut(s) | 262 |
| EcoT38I | GRGCYC | 1 cut(s) | 362 |
| FaeI | CATG | 7 cut(s) | 172, 232, 318, 589, 692, 736, 940 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 882, 914, 1081, 1113 |
| FatI | CATG | 7 cut(s) | 168, 228, 314, 585, 688, 732, 936 |
| Fnu4HI | GCNGC | 3 cut(s) | 65, 595, 615 |
| FokI | GGATG | 2 cut(s) | 296, 500 |
| FriOI | GRGCYC | 1 cut(s) | 362 |
| Fsp4HI | GCNGC | 3 cut(s) | 65, 595, 615 |
| FspBI | CTAG | 1 cut(s) | 747 |
| GluI | GCNGC | 3 cut(s) | 65, 595, 615 |
| GsuI | CTGGAG | 1 cut(s) | 122 |
| HaeIII | GGCC | 1 cut(s) | 737 |
| HapII | CCGG | 1 cut(s) | 591 |
| Hin1II | CATG | 7 cut(s) | 172, 232, 318, 589, 692, 736, 940 |
| HindIII | AAGCTT | 1 cut(s) | 557 |
| HinfI | GANTC | 5 cut(s) | 174, 199, 308, 551, 823 |
| HpaII | CCGG | 1 cut(s) | 591 |
| HphI | GGTGA | 2 cut(s) | 780, 848 |
| Hpy166II | GTNNAC | 2 cut(s) | 238, 320 |
| Hpy188I | TCNGA | 4 cut(s) | 339, 364, 540, 756 |
| Hpy188III | TCNNGA | 6 cut(s) | 178, 563, 586, 628, 747, 794 |
| Hpy8I | GTNNAC | 2 cut(s) | 238, 320 |
| HpyAV | CCTTC | 2 cut(s) | 42, 913 |
| HpyCH4III | ACNGT | 1 cut(s) | 845 |
| HpyCH4IV | ACGT | 3 cut(s) | 108, 234, 468 |
| HpyCH4V | TGCA | 9 cut(s) | 64, 232, 260, 314, 579, 597, 976, 1044, 1111 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 169 |
| HpyF3I | CTNAG | 1 cut(s) | 755 |
| HpySE526I | ACGT | 3 cut(s) | 108, 234, 468 |
| Hsp92II | CATG | 7 cut(s) | 172, 232, 318, 589, 692, 736, 940 |
| Kzo9I | GATC | 2 cut(s) | 565, 722 |
| Lsp1109I | GCAGC | 2 cut(s) | 76, 581 |
| LweI | GCATC | 2 cut(s) | 274, 588 |
| MaeI | CTAG | 1 cut(s) | 747 |
| MaeII | ACGT | 3 cut(s) | 108, 234, 468 |
| MaeIII | GTNAC | 3 cut(s) | 140, 341, 786 |
| MalI | GATC | 2 cut(s) | 567, 724 |
| MboI | GATC | 2 cut(s) | 565, 722 |
| MflI | RGATCY | 1 cut(s) | 722 |
| MhlI | GDGCHC | 1 cut(s) | 362 |
| MlyI | GAGTC | 1 cut(s) | 302 |
| Mph1103I | ATGCAT | 1 cut(s) | 262 |
| MseI | TTAA | 2 cut(s) | 186, 927 |
| MslI | CAYNNNNRTG | 4 cut(s) | 87, 233, 584, 731 |
| MspI | CCGG | 1 cut(s) | 591 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 169 |
| NdeII | GATC | 2 cut(s) | 565, 722 |
| NlaIII | CATG | 7 cut(s) | 172, 232, 318, 589, 692, 736, 940 |
| NlaIV | GGNNCC | 1 cut(s) | 724 |
| NmuCI | GTSAC | 1 cut(s) | 786 |
| NsiI | ATGCAT | 1 cut(s) | 262 |
| NspI | RCATGY | 3 cut(s) | 172, 232, 318 |
| PagI | TCATGA | 1 cut(s) | 585 |
| PfeI | GAWTC | 4 cut(s) | 174, 199, 551, 823 |
| PkrI | GCNGC | 3 cut(s) | 66, 596, 616 |
| PleI | GAGTC | 1 cut(s) | 302 |
| PmaCI | CACGTG | 1 cut(s) | 235 |
| PmlI | CACGTG | 1 cut(s) | 235 |
| PpsI | GAGTC | 1 cut(s) | 302 |
| Ppu21I | YACGTR | 2 cut(s) | 109, 235 |
| PsiI | TTATAA | 1 cut(s) | 210 |
| Psp124BI | GAGCTC | 1 cut(s) | 362 |
| PspCI | CACGTG | 1 cut(s) | 235 |
| PspN4I | GGNNCC | 1 cut(s) | 724 |
| PspPI | GGNCC | 1 cut(s) | 667 |
| PstI | CTGCAG | 2 cut(s) | 66, 599 |
| PsuI | RGATCY | 1 cut(s) | 722 |
| RsaI | GTAC | 5 cut(s) | 107, 321, 334, 467, 719 |
| RsaNI | GTAC | 5 cut(s) | 106, 320, 333, 466, 718 |
| RseI | CAYNNNNRTG | 4 cut(s) | 87, 233, 584, 731 |
| SacI | GAGCTC | 1 cut(s) | 362 |
| SaqAI | TTAA | 2 cut(s) | 186, 927 |
| SatI | GCNGC | 3 cut(s) | 65, 595, 615 |
| Sau3AI | GATC | 2 cut(s) | 565, 722 |
| Sau96I | GGNCC | 1 cut(s) | 667 |
| SchI | GAGTC | 1 cut(s) | 302 |
| SduI | GDGCHC | 1 cut(s) | 362 |
| SfaNI | GCATC | 2 cut(s) | 274, 588 |
| SfcI | CTRYAG | 2 cut(s) | 62, 595 |
| SinI | GGWCC | 1 cut(s) | 667 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 87, 233, 584, 731 |
| SmlI | CTYRAG | 2 cut(s) | 346, 792 |
| SmoI | CTYRAG | 2 cut(s) | 346, 792 |
| SsiI | CCGC | 2 cut(s) | 287, 614 |
| SspI | AATATT | 3 cut(s) | 624, 1012, 1081 |
| SspMI | CTAG | 1 cut(s) | 747 |
| SstI | GAGCTC | 1 cut(s) | 362 |
| TaaI | ACNGT | 1 cut(s) | 845 |
| TaiI | ACGT | 3 cut(s) | 111, 237, 471 |
| TaqI | TCGA | 1 cut(s) | 619 |
| TatI | WGTACW | 2 cut(s) | 319, 332 |
| TauI | GCSGC | 1 cut(s) | 617 |
| TfiI | GAWTC | 4 cut(s) | 174, 199, 551, 823 |
| Tru1I | TTAA | 2 cut(s) | 186, 927 |
| Tru9I | TTAA | 2 cut(s) | 186, 927 |
| TscAI | CASTG | 1 cut(s) | 581 |
| TseFI | GTSAC | 1 cut(s) | 786 |
| TseI | GCWGC | 2 cut(s) | 64, 594 |
| Tsp45I | GTSAC | 1 cut(s) | 786 |
| TspDTI | ATGAA | 1 cut(s) | 131 |
| TspGWI | ACGGA | 4 cut(s) | 296, 500, 689, 735 |
| TspRI | CASTG | 1 cut(s) | 581 |
| VpaK11BI | GGWCC | 1 cut(s) | 667 |
| XapI | RAATTY | 3 cut(s) | 699, 849, 929 |
| XbaI | TCTAGA | 1 cut(s) | 746 |
| XceI | RCATGY | 3 cut(s) | 172, 232, 318 |
| XspI | CTAG | 1 cut(s) | 747 |
| Zsp2I | ATGCAT | 1 cut(s) | 262 |