Rh4AG189600

L-type lectin-domain containing receptor kinase IX.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0023160
Physical Location & Seq
Forward (+)
47026977 .. 47042399
15423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG189600.1

Sequence Viewer

Length: 783 bp
ATGTCTGTAAGCAGAAGCAACCAGGCAATCTGCTTGTGCAATCTTTGCTTCTTAAGTTTCATAATTTTTTTCCCTCACTTTTCTCATCCACTTTCCTTCAGCATAAATCAATTCAATGCTACTACAAAGGGAGTTGTCTACCAAGGTGATGCCTGGCCTTCGTCGGAAGTGATTGAGCTCAACAAAATAACTTATAAATTCCGTGTTGGACCGGCTATGTATGCCGAGCCTTTGCACTTATGGGACAGTACTACTGGGTCACTCGCCGACTTCAGTACTCATTTCACTTTCATGGTCGACACTGGTAATAGCGAAATCTTCAGTGATGGAGTTGCCTTTTTCCTATCTCCCGTGGGTTATCAGTTTCTGCCATACTTTTCAGTTGGTGGTCTAGGACTCTTCAACACCCACGCCAGTACTCTTAATGCTTCCCAAAGCCAAGTTGTAAGCATGGAGTTTGATACTTGGTCGGACAACGAATGGGATCCACCAGGACCTCATCTTAGGATCAATATCAAAGATATCTCTTCAGTTGTTTACGCCGATTGGAATGTTAAATGCTTTGAGAATCGGTGTTTAGAATTTATGACCAATTTGCAAATTCATGTCGCTTCAATTCTATCCCAAGACGAGTTGAAGAAAATCGCCGCCTACAAGGTCGTCGAGTCCGGCATGAACTTTGGCCTCGGCTCCGGTTCCACCATCAAACACATCGTGGATCGCATCGGCGAGCTTTTGCAACTGGGAAAAGTTCACAACATCATCGGAATCACCTCTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

260

Amino Acids

28.93

Weight (kDa)

5.51

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 31 - 185 1.5e-35 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023160)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8393587.1_g000001
rosa_samantha Rh4AG189600 Rh4DG188300
rosa_wichuraiana Rw4G016170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AccI GTMKAC 2 cut(s) 138, 297
AciI CCGC 1 cut(s) 648
AclWI GGATC 4 cut(s) 479, 492, 515, 726
AcsI RAATTY 3 cut(s) 197, 581, 600
AcuI CTGAAG 4 cut(s) 82, 256, 304, 513
AdeI CACNNNGTG 1 cut(s) 715
AfaI GTAC 3 cut(s) 250, 277, 418
AflII CTTAAG 1 cut(s) 52
AgsI TTSAA 4 cut(s) 115, 403, 615, 637
AjnI CCWGG 3 cut(s) 21, 152, 490
AluBI AGCT 2 cut(s) 178, 733
AluI AGCT 2 cut(s) 178, 733
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 4 cut(s) 479, 492, 515, 726
AlwNI CAGNNNCTG 1 cut(s) 367
AoxI GGCC 2 cut(s) 155, 682
ApoI RAATTY 3 cut(s) 197, 581, 600
AspS9I GGNCC 2 cut(s) 209, 494
AsuHPI GGTGA 2 cut(s) 158, 763
AvaII GGWCC 2 cut(s) 209, 494
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BccI CCATC 2 cut(s) 320, 710
BciT130I CCWGG 3 cut(s) 23, 154, 492
BfaI CTAG 2 cut(s) 392, 781
BfrI CTTAAG 1 cut(s) 52
BisI GCNGC 1 cut(s) 648
BlsI GCNGC 1 cut(s) 649
BmcAI AGTACT 3 cut(s) 250, 277, 418
Bme1390I CCNGG 3 cut(s) 23, 154, 492
Bme18I GGWCC 2 cut(s) 209, 494
BmgT120I GGNCC 2 cut(s) 209, 494
BmiI GGNNCC 3 cut(s) 486, 691, 697
BmrFI CCNGG 3 cut(s) 23, 154, 492
BmrI ACTGGG 2 cut(s) 264, 752
BmsI GCATC 2 cut(s) 139, 732
BmuI ACTGGG 2 cut(s) 264, 752
BsaBI GATNNNNATC 1 cut(s) 512
BsaJI CCNNGG 3 cut(s) 142, 351, 685
BsaWI WCCGGW 1 cut(s) 692
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 4 cut(s) 259, 307, 414, 747
Bse8I GATNNNNATC 1 cut(s) 512
BseBI CCWGG 3 cut(s) 23, 154, 492
BseDI CCNNGG 3 cut(s) 142, 351, 685
BseGI GGATG 1 cut(s) 85
BseJI GATNNNNATC 1 cut(s) 512
BseNI ACTGG 4 cut(s) 259, 307, 414, 747
BshFI GGCC 2 cut(s) 157, 684
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 3 cut(s) 212, 669, 693
BslFI GGGAC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 2 cut(s) 157, 684
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 484, 507, 718
BspACI CCGC 1 cut(s) 648
BspANI GGCC 2 cut(s) 157, 684
BspLI GGNNCC 3 cut(s) 486, 691, 697
BspPI GGATC 4 cut(s) 479, 492, 515, 726
BspTI CTTAAG 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 4 cut(s) 259, 307, 414, 747
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 3 cut(s) 142, 351, 685
BssMI GATC 3 cut(s) 484, 507, 718
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 3 cut(s) 23, 154, 492
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 2 cut(s) 404, 532
BstAFI CTTAAG 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 731
BstDEI CTNAG 1 cut(s) 503
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 3 cut(s) 487, 510, 721
BstMBI GATC 3 cut(s) 484, 507, 718
BstMWI GCNNNNNNNGC 2 cut(s) 45, 221
BstNI CCWGG 3 cut(s) 23, 154, 492
BstSCI CCNGG 3 cut(s) 21, 152, 490
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 2 cut(s) 157, 684
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 300, 328
Cac8I GCNNGC 1 cut(s) 731
CaiI CAGNNNCTG 1 cut(s) 367
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 209, 494
Csp6I GTAC 3 cut(s) 249, 276, 417
CviAII CATG 4 cut(s) 292, 451, 605, 673
CviJI RGCY 8 cut(s) 157, 178, 215, 229, 438, 684, 690, 733
CviKI_1 RGCY 8 cut(s) 157, 178, 215, 229, 438, 684, 690, 733
CviQI GTAC 3 cut(s) 249, 276, 417
DdeI CTNAG 1 cut(s) 503
DpnI GATC 3 cut(s) 486, 509, 720
DpnII GATC 3 cut(s) 484, 507, 718
DraIII CACNNNGTG 1 cut(s) 715
Eam1104I CTCTTC 2 cut(s) 404, 532
EarI CTCTTC 2 cut(s) 404, 532
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 2 cut(s) 209, 494
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 4 cut(s) 82, 256, 304, 513
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 494
EcoRII CCWGG 3 cut(s) 21, 152, 490
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 4 cut(s) 295, 454, 608, 676
FaqI GGGAC 1 cut(s) 257
FatI CATG 4 cut(s) 291, 450, 604, 672
FblI GTMKAC 2 cut(s) 138, 297
Fnu4HI GCNGC 1 cut(s) 648
FokI GGATG 1 cut(s) 72
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 1 cut(s) 648
FspBI CTAG 2 cut(s) 392, 781
GluI GCNGC 1 cut(s) 648
HaeIII GGCC 2 cut(s) 157, 684
HapII CCGG 3 cut(s) 212, 669, 693
Hin1II CATG 4 cut(s) 295, 454, 608, 676
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 4 cut(s) 396, 568, 665, 768
HpaII CCGG 3 cut(s) 212, 669, 693
HphI GGTGA 2 cut(s) 158, 763
Hpy166II GTNNAC 4 cut(s) 139, 298, 538, 754
Hpy188I TCNGA 3 cut(s) 166, 472, 767
Hpy8I GTNNAC 4 cut(s) 139, 298, 538, 754
Hpy99I CGWCG 2 cut(s) 166, 665
HpyAV CCTTC 2 cut(s) 106, 168
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 4 cut(s) 39, 235, 598, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 221
HpyF3I CTNAG 1 cut(s) 503
Hsp92II CATG 4 cut(s) 295, 454, 608, 676
Kzo9I GATC 3 cut(s) 484, 507, 718
LmnI GCTCC 1 cut(s) 695
LweI GCATC 2 cut(s) 139, 732
MaeI CTAG 2 cut(s) 392, 781
MaeIII GTNAC 1 cut(s) 258
MalI GATC 3 cut(s) 486, 509, 720
MboI GATC 3 cut(s) 484, 507, 718
MboII GAAGA 4 cut(s) 310, 391, 519, 649
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 7 cut(s) 63, 110, 197, 581, 592, 600, 615
MlyI GAGTC 2 cut(s) 390, 674
MmeI TCCRAC 3 cut(s) 144, 187, 450
MnlI CCTC 3 cut(s) 84, 507, 695
MseI TTAA 3 cut(s) 53, 423, 555
MslI CAYNNNNRTG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 52
MspI CCGG 3 cut(s) 212, 669, 693
MspR9I CCNGG 3 cut(s) 23, 154, 492
MvaI CCWGG 3 cut(s) 23, 154, 492
MwoI GCNNNNNNNGC 2 cut(s) 45, 221
NdeII GATC 3 cut(s) 484, 507, 718
NlaIII CATG 4 cut(s) 295, 454, 608, 676
NlaIV GGNNCC 3 cut(s) 486, 691, 697
NmeAIII GCCGAG 2 cut(s) 250, 666
NmuCI GTSAC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 568, 768
PkrI GCNGC 1 cut(s) 649
PleI GAGTC 2 cut(s) 390, 673
PpsI GAGTC 2 cut(s) 390, 673
PpuMI RGGWCCY 1 cut(s) 494
PsiI TTATAA 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 494
Psp6I CCWGG 3 cut(s) 21, 152, 490
PspGI CCWGG 3 cut(s) 21, 152, 490
PspN4I GGNNCC 3 cut(s) 486, 691, 697
PspPI GGNCC 2 cut(s) 209, 494
PspPPI RGGWCCY 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 3 cut(s) 250, 277, 418
RsaNI GTAC 3 cut(s) 249, 276, 417
RseI CAYNNNNRTG 1 cut(s) 290
SacI GAGCTC 1 cut(s) 180
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 3 cut(s) 53, 423, 555
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 3 cut(s) 484, 507, 718
Sau96I GGNCC 2 cut(s) 209, 494
ScaI AGTACT 3 cut(s) 250, 277, 418
SchI GAGTC 2 cut(s) 390, 674
ScrFI CCNGG 3 cut(s) 23, 154, 492
SduI GDGCHC 1 cut(s) 180
SetI ASST 6 cut(s) 148, 180, 499, 660, 735, 776
SfaNI GCATC 2 cut(s) 139, 732
SinI GGWCC 2 cut(s) 209, 494
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 7 cut(s) 63, 110, 197, 581, 592, 600, 615
SsiI CCGC 1 cut(s) 648
SspMI CTAG 2 cut(s) 392, 781
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 21, 152, 490
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 2 cut(s) 297, 663
TasI AATT 7 cut(s) 63, 110, 197, 581, 592, 600, 615
TatI WGTACW 3 cut(s) 248, 275, 416
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 2 cut(s) 568, 768
Tru1I TTAA 3 cut(s) 53, 423, 555
Tru9I TTAA 3 cut(s) 53, 423, 555
TscAI CASTG 2 cut(s) 307, 328
TseFI GTSAC 1 cut(s) 258
Tsp45I GTSAC 1 cut(s) 258
TspDTI ATGAA 4 cut(s) 49, 280, 593, 689
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 307, 328
Vha464I CTTAAG 1 cut(s) 52
VpaK11BI GGWCC 2 cut(s) 209, 494
XapI RAATTY 3 cut(s) 197, 581, 600
XmiI GTMKAC 2 cut(s) 138, 297
XspI CTAG 2 cut(s) 392, 781
ZrmI AGTACT 3 cut(s) 250, 277, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.