Rw4G016170

L-type lectin-domain containing receptor kinase IX.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0023160
Physical Location & Seq
Forward (+)
37106545 .. 37107309
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016170.1

Sequence Viewer

Length: 765 bp
ATGTCTGTAAGCAGAAGCAACCAGGCAATCTGCTTGTGCAATCTTTGCTTCTTAAGTTTCATAATTTTTTTCCCTCACTTTTCTCATCCACTTTCCTTCAGCATAAATCAATTCAATGCTACTACAAAGGGAGTTGTCTACCAAGGTGATGCCTGGCCTTCGTCGGAAGTGATTGAGCTCAACAAAATAACTTATAAATTCCGTGTTGGACGGGCTATGTATGCCGAGCCTTTGCACTTATGGGACAGTACTACTGGGTCACTCGCCGACTTCAGTACTCATTTCACTTTCATGGTCGACACTGGTAATAGCGAAATCTTCAGTGATGGAGTTGCCTTTTTCCTATCTCCCGTGGGTTATCAGTTTCTGCCATACTTTTCAGTTGGTGGTCTAGGACTCTTCAACACCCACGCCAGTACTCTTAATGCTTCCCAAAGCCAAGTTGTAAGCATGGAGTTTGATACTTGGTCGGACAACGAATGGGATCCACCAGGACCTCATCTTAGGATCAATATCAAAGATATCTCTTCAGTTGTTTACGCCTATTGGAATGTTAGCATCATCTCGGGGAAGCTTGAAAATGCATGGATAACTTACAACGCTACTGCCAACAACCTTACTATGTTTTGGAATTTCACTGAGAAATTCAACCCTGCGTTTTTGGGGATGAATGCTTCTTCTATCATTTACCATGCTAACTTGAGAAAGGTTCTTACCGAATGGGTTACAGTCGATTTTTCTGCTTCAAGTGGAGAATTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

254

Amino Acids

28.55

Weight (kDa)

5.23

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 31 - 252 8.9e-51 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023160)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8393587.1_g000001
rosa_samantha Rh4AG189600 Rh4DG188300
rosa_wichuraiana Rw4G016170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AccI GTMKAC 2 cut(s) 138, 297
AclWI GGATC 3 cut(s) 479, 492, 515
AcsI RAATTY 4 cut(s) 197, 631, 644, 755
AcuI CTGAAG 4 cut(s) 82, 256, 304, 513
AfaI GTAC 3 cut(s) 250, 277, 418
AflII CTTAAG 1 cut(s) 52
AgsI TTSAA 5 cut(s) 115, 403, 578, 649, 747
AjnI CCWGG 3 cut(s) 21, 152, 490
AluBI AGCT 2 cut(s) 178, 574
AluI AGCT 2 cut(s) 178, 574
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 3 cut(s) 479, 492, 515
AlwNI CAGNNNCTG 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 1 cut(s) 155
ApoI RAATTY 4 cut(s) 197, 631, 644, 755
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 1 cut(s) 494
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BccI CCATC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 3 cut(s) 23, 154, 492
BfaI CTAG 1 cut(s) 392
BfrI CTTAAG 1 cut(s) 52
BmcAI AGTACT 3 cut(s) 250, 277, 418
Bme1390I CCNGG 3 cut(s) 23, 154, 492
Bme18I GGWCC 1 cut(s) 494
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 486
BmrFI CCNGG 3 cut(s) 23, 154, 492
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 139, 567
BmuI ACTGGG 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 721
BsaBI GATNNNNATC 1 cut(s) 512
BsaJI CCNNGG 2 cut(s) 142, 351
Bse1I ACTGG 3 cut(s) 259, 307, 414
Bse8I GATNNNNATC 1 cut(s) 512
BseBI CCWGG 3 cut(s) 23, 154, 492
BseDI CCNNGG 2 cut(s) 142, 351
BseGI GGATG 2 cut(s) 85, 672
BseJI GATNNNNATC 1 cut(s) 512
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 3 cut(s) 259, 307, 414
BshFI GGCC 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 565
BslFI GGGAC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 565
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 484, 507
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 486
BspPI GGATC 3 cut(s) 479, 492, 515
BspTI CTTAAG 1 cut(s) 52
BsrI ACTGG 3 cut(s) 259, 307, 414
BssECI CCNNGG 2 cut(s) 142, 351
BssMI GATC 2 cut(s) 484, 507
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 3 cut(s) 23, 154, 492
Bst4CI ACNGT 2 cut(s) 248, 730
Bst6I CTCTTC 2 cut(s) 404, 532
BstAFI CTTAAG 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 503, 639, 762
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 85, 672
BstKTI GATC 2 cut(s) 487, 510
BstMBI GATC 2 cut(s) 484, 507
BstMWI GCNNNNNNNGC 2 cut(s) 45, 221
BstNI CCWGG 3 cut(s) 23, 154, 492
BstSCI CCNGG 3 cut(s) 21, 152, 490
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 1 cut(s) 157
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 85, 672
BtsIMutI CAGTG 3 cut(s) 300, 328, 636
CaiI CAGNNNCTG 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 494
Csp6I GTAC 3 cut(s) 249, 276, 417
CviAII CATG 4 cut(s) 292, 451, 585, 692
CviJI RGCY 6 cut(s) 157, 178, 215, 229, 438, 574
CviKI_1 RGCY 6 cut(s) 157, 178, 215, 229, 438, 574
CviQI GTAC 3 cut(s) 249, 276, 417
DdeI CTNAG 3 cut(s) 503, 639, 762
DpnI GATC 2 cut(s) 486, 509
DpnII GATC 2 cut(s) 484, 507
Eam1104I CTCTTC 2 cut(s) 404, 532
EarI CTCTTC 2 cut(s) 404, 532
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 4 cut(s) 82, 256, 304, 513
Eco88I CYCGRG 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 3 cut(s) 21, 152, 490
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 4 cut(s) 295, 454, 588, 695
FaqI GGGAC 1 cut(s) 257
FatI CATG 4 cut(s) 291, 450, 584, 691
FblI GTMKAC 2 cut(s) 138, 297
FokI GGATG 2 cut(s) 72, 679
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 1 cut(s) 392
HaeIII GGCC 1 cut(s) 157
Hin1II CATG 4 cut(s) 295, 454, 588, 695
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 1 cut(s) 396
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 3 cut(s) 139, 298, 538
Hpy188I TCNGA 2 cut(s) 166, 472
Hpy8I GTNNAC 3 cut(s) 139, 298, 538
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 106, 168
HpyCH4III ACNGT 2 cut(s) 248, 730
HpyCH4V TGCA 3 cut(s) 39, 235, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 221
HpyF3I CTNAG 3 cut(s) 503, 639, 762
Hsp92II CATG 4 cut(s) 295, 454, 588, 695
Kzo9I GATC 2 cut(s) 484, 507
LweI GCATC 2 cut(s) 139, 567
MaeI CTAG 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 258, 724
MalI GATC 2 cut(s) 486, 509
MboI GATC 2 cut(s) 484, 507
MboII GAAGA 4 cut(s) 310, 391, 519, 669
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 6 cut(s) 63, 110, 197, 631, 644, 755
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 3 cut(s) 144, 187, 450
MnlI CCTC 2 cut(s) 84, 507
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 53, 423
MslI CAYNNNNRTG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 52
MspR9I CCNGG 3 cut(s) 23, 154, 492
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 3 cut(s) 23, 154, 492
MwoI GCNNNNNNNGC 2 cut(s) 45, 221
NdeII GATC 2 cut(s) 484, 507
NlaIII CATG 4 cut(s) 295, 454, 588, 695
NlaIV GGNNCC 1 cut(s) 486
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 586
PctI GAATGC 1 cut(s) 676
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 494
PsiI TTATAA 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 494
Psp6I CCWGG 3 cut(s) 21, 152, 490
PspGI CCWGG 3 cut(s) 21, 152, 490
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 1 cut(s) 494
PspPPI RGGWCCY 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 3 cut(s) 250, 277, 418
RsaNI GTAC 3 cut(s) 249, 276, 417
RseI CAYNNNNRTG 1 cut(s) 290
SacI GAGCTC 1 cut(s) 180
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 53, 423
Sau3AI GATC 2 cut(s) 484, 507
Sau96I GGNCC 1 cut(s) 494
ScaI AGTACT 3 cut(s) 250, 277, 418
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 3 cut(s) 23, 154, 492
SduI GDGCHC 1 cut(s) 180
SetI ASST 6 cut(s) 148, 180, 499, 576, 618, 711
SfaNI GCATC 2 cut(s) 139, 567
SinI GGWCC 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 52, 700
SmoI CTYRAG 2 cut(s) 52, 700
Sse9I AATT 6 cut(s) 63, 110, 197, 631, 644, 755
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 21, 152, 490
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 248, 730
TaqI TCGA 2 cut(s) 297, 732
TasI AATT 6 cut(s) 63, 110, 197, 631, 644, 755
TatI WGTACW 3 cut(s) 248, 275, 416
Tru1I TTAA 2 cut(s) 53, 423
Tru9I TTAA 2 cut(s) 53, 423
TscAI CASTG 3 cut(s) 307, 328, 643
TseFI GTSAC 1 cut(s) 258
Tsp45I GTSAC 1 cut(s) 258
TspDTI ATGAA 3 cut(s) 49, 280, 683
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 307, 328, 643
Vha464I CTTAAG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 4 cut(s) 197, 631, 644, 755
XmiI GTMKAC 2 cut(s) 138, 297
XspI CTAG 1 cut(s) 392
ZrmI AGTACT 3 cut(s) 250, 277, 418
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.