Rh4AG340400

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006846
Physical Location & Seq
Forward (+)
64980744 .. 64986276
5533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG340400.1

Sequence Viewer

Length: 1452 bp
ATGCAGGGAATGATTAGCCTGACAGTGTCGGCAGTACTTCCCCAACTGAGGCCTCCCCCATGTGACCACAACCAAGTGTGCCAAGAAGCCGACGGTGGACAACTTGCAATTCTTTACATATCTCTCTTACTAGGAGCACTCGGGTCCGGTGGGATCCGACCCTGCGTTGTGGCATTCGGAGCGGACCAGTTTGACGAGAGCGATCCGAAGCAAAACGCCAAGACATGGAGCTACTTTAACTGGTACTACTTTGTGATGGGAGCATCCATGCTAGTTGCTGTGACAGTGCTAGTGTACATCCAGGACAATATCGGATGGGGTTGGGGGCTTGGAATTCCAACTATAGCCATGTTCCTTTCGGTTATTACATTTGTGATAGGGTACCCGCTTTATCGACACTTGGATCCGGCTGGAAGTCCGTTTACCCGGTTGGCACAAGTGTTAGTGGCCGCTTGCAAGAAGAGAAAGGTACCTATGGTTTCTGATCCTAGATTGCTGTATCAGAATGATGAACTTGACGATCCTATTTCTATTGGTGGCAAGCTTCTCCATACTAATCATATGAAATTTCTTGACAAGGCAGCCATTGTGGCAGAGGAAGACGAACTCAAGTCACCCAACCTGTGGAGGATCAGCACCGTTCATCGCGTGGAAGAACTGAAATCAGTGATCAGAATGGGACCCATATGGGCAGCAGGCATACTCCTAATTACAGCCTATTCCCAACAAAACACTTTCTCACTCAGCCAAGCAAAAACCATGAACAGACACCTCTCAAAGTCCTTTGAAATCCCAGCAGCCTCCATGTCAGTGTTTACCATGGTCCCTATGCTCTTAACAATCGCATTCTACGACCGAGTTTTCATACCCCTAGCCAGAAAGGTGACGGGTTTGGACAGGGGGATCACATTCCTTCACAGAATGGGAATCGGATTCGTCATTTCCATACTGGCAACTTTCGTCGCTGGATTCGTGGAATTGAAGCGAAAAAAGGCAGCATTGGCTTATGGGCTTCTGAATCAGCCACACACCATGATCCCCATTTCAGTATTTTGGCTAGCGCCCCAGTACAGCCTTCATGGGATAGCTGAAGCATTCATGTCAATCGGGCACCTAGAGTTTTTCTACAGCCAGGCGCCAGAGAGCATGAGGAGCACTGCTGCTGCACTGTTCTGGACTGCGATATCGGCCGGGAATTATTTGAGCACCCTCTTGGTTTCATTGGTGCTCAAGTTTAGTGCTGGGACTAATGGATCCAACTGGCTACCGGATGATAATTTGAACCAGGGGAAGCTGGAAAACTTTTACTGGTTGATCACCTTGCTACAAGTTGCTAATCTGATTTACTACCTTTGGTGCGCTAAAATATATACGTATAAGCCGATTCAGGTGAAAGGGAAAGATGGAGCTGAGTCTGAAGGAAAGGGTGTGGAGCTTTCTATTCGGGTTTAG

Protein Analysis

483

Amino Acids

53.58

Weight (kDa)

8.75

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 3 - 421 2.4e-94 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 381, 469
AccB1I GGYRCC 4 cut(s) 381, 469, 1110, 1135
AccB7I CCANNNNNTGG 2 cut(s) 225, 624
AccBSI CCGCTC 1 cut(s) 182
AccII CGCG 1 cut(s) 648
AciI CCGC 3 cut(s) 182, 386, 450
AcoI YGGCCR 2 cut(s) 447, 1188
AcsI RAATTY 2 cut(s) 333, 566
AcuI CTGAAG 2 cut(s) 1110, 1437
AcyI GRCGYC 1 cut(s) 1136
AfaI GTAC 6 cut(s) 36, 245, 296, 383, 471, 1070
AfiI CCNNNNNNNGG 3 cut(s) 48, 225, 624
AgsI TTSAA 3 cut(s) 788, 982, 1282
AjnI CCWGG 3 cut(s) 300, 1131, 1284
AluBI AGCT 6 cut(s) 231, 544, 1088, 1294, 1409, 1435
AluI AGCT 6 cut(s) 231, 544, 1088, 1294, 1409, 1435
Alw21I GWGCWC 4 cut(s) 139, 1157, 1208, 1230
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 3 cut(s) 50, 447, 1188
ApeKI GCWGC 6 cut(s) 581, 692, 797, 995, 1160, 1163
ApoI RAATTY 2 cut(s) 333, 566
Asp718I GGTACC 2 cut(s) 381, 469
AspLEI GCGC 3 cut(s) 1063, 1138, 1361
AspS9I GGNCC 4 cut(s) 144, 184, 680, 823
AsuC2I CCSGG 2 cut(s) 427, 1192
AsuHPI GGTGA 4 cut(s) 606, 895, 1309, 1402
AsuNHI GCTAGC 1 cut(s) 1057
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 4 cut(s) 144, 184, 680, 823
BaeGI GKGCMC 1 cut(s) 1113
BamHI GGATCC 3 cut(s) 153, 403, 1253
BanI GGYRCC 4 cut(s) 381, 469, 1110, 1135
BarI GAAGNNNNNNTAC 2 cut(s) 406, 438
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 4 cut(s) 139, 1157, 1208, 1230
BbvI GCAGC 6 cut(s) 593, 704, 809, 1007, 1147, 1150
BccI CCATC 3 cut(s) 250, 309, 1397
BciT130I CCWGG 3 cut(s) 302, 1133, 1286
BclI TGATCA 2 cut(s) 669, 1314
BcnI CCSGG 2 cut(s) 427, 1192
BfaI CTAG 7 cut(s) 131, 272, 290, 489, 872, 1058, 1115
BfmI CTRYAG 2 cut(s) 342, 1126
BfoI RGCGCY 2 cut(s) 1064, 1139
BglI GCCNNNNNGGC 1 cut(s) 590
BisI GCNGC 7 cut(s) 450, 582, 693, 798, 996, 1161, 1164
BlsI GCNGC 7 cut(s) 451, 583, 694, 799, 997, 1162, 1165
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 5 cut(s) 302, 427, 1133, 1192, 1286
Bme18I GGWCC 4 cut(s) 144, 184, 680, 823
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 4 cut(s) 144, 184, 680, 823
BmrFI CCNGG 5 cut(s) 302, 427, 1133, 1192, 1286
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 1 cut(s) 272
BmtI GCTAGC 1 cut(s) 1061
BmuI ACTGGG 1 cut(s) 1060
BpiI GAAGAC 1 cut(s) 606
BpuEI CTTGAG 2 cut(s) 593, 1214
BpuMI CCSGG 2 cut(s) 427, 1192
BsaAI YACGTR 1 cut(s) 1374
BsaHI GRCGYC 1 cut(s) 1136
BsaJI CCNNGG 2 cut(s) 819, 1285
BsaWI WCCGGW 2 cut(s) 146, 1267
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 225, 624
Bse1I ACTGG 6 cut(s) 187, 245, 954, 1066, 1265, 1313
BseBI CCWGG 3 cut(s) 302, 1133, 1286
BseDI CCNNGG 2 cut(s) 819, 1285
BseGI GGATG 4 cut(s) 263, 297, 320, 1276
BseLI CCNNNNNNNGG 3 cut(s) 48, 225, 624
BseMII CTCAG 3 cut(s) 38, 757, 1401
BseNI ACTGG 6 cut(s) 187, 245, 954, 1066, 1265, 1313
BseRI GAGGAG 1 cut(s) 1165
BseSI GKGCMC 1 cut(s) 1113
BseX3I CGGCCG 1 cut(s) 1188
BseXI GCAGC 6 cut(s) 593, 704, 809, 1007, 1147, 1150
BseYI CCCAGC 2 cut(s) 793, 1241
BsgI GTGCAG 1 cut(s) 1149
Bsh1236I CGCG 1 cut(s) 648
Bsh1285I CGRYCG 2 cut(s) 856, 1191
BshFI GGCC 3 cut(s) 52, 449, 1190
BshNI GGYRCC 4 cut(s) 381, 469, 1110, 1135
BsiEI CGRYCG 2 cut(s) 856, 1191
BsiHKAI GWGCWC 4 cut(s) 139, 1157, 1208, 1230
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 5 cut(s) 147, 407, 427, 1191, 1268
BslFI GGGAC 3 cut(s) 693, 809, 1258
BslI CCNNNNNNNGG 3 cut(s) 48, 225, 624
BsmFI GGGAC 3 cut(s) 693, 809, 1258
BsmI GAATGC 3 cut(s) 173, 845, 1094
BsnI GGCC 3 cut(s) 52, 449, 1190
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 5 cut(s) 139, 1113, 1157, 1208, 1230
Bsp1407I TGTACA 1 cut(s) 294
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 3 cut(s) 182, 386, 450
BspANI GGCC 3 cut(s) 52, 449, 1190
BspCNI CTCAG 3 cut(s) 39, 756, 1402
BspFNI CGCG 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 1061
BspT107I GGYRCC 4 cut(s) 381, 469, 1110, 1135
BsrBI CCGCTC 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 6 cut(s) 187, 245, 954, 1066, 1265, 1313
BssECI CCNNGG 2 cut(s) 819, 1285
BssNI GRCGYC 1 cut(s) 1136
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 302, 1133, 1286
Bst4CI ACNGT 5 cut(s) 25, 95, 286, 640, 1170
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 1136
BstAUI TGTACA 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 1374
BstC8I GCNNGC 4 cut(s) 454, 542, 697, 1059
BstDEI CTNAG 3 cut(s) 47, 743, 1410
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 4 cut(s) 263, 297, 320, 1276
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 2 cut(s) 1064, 1139
BstHHI GCGC 3 cut(s) 1063, 1138, 1361
BstMCI CGRYCG 2 cut(s) 856, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 179, 590, 1001, 1152, 1187
BstNI CCWGG 3 cut(s) 302, 1133, 1286
BstSCI CCNGG 5 cut(s) 300, 425, 1131, 1190, 1284
BstSFI CTRYAG 2 cut(s) 342, 1126
BstSLI GKGCMC 1 cut(s) 1113
BstSNI TACGTA 1 cut(s) 1374
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 6 cut(s) 593, 704, 809, 1007, 1147, 1150
BstV2I GAAGAC 1 cut(s) 606
BstX2I RGATCY 3 cut(s) 153, 403, 1253
BstYI RGATCY 3 cut(s) 153, 403, 1253
BstZI CGGCCG 1 cut(s) 1188
BsuRI GGCC 3 cut(s) 52, 449, 1190
BtgI CCRYGG 1 cut(s) 819
BtgZI GCGATG 1 cut(s) 629
BtsCI GGATG 4 cut(s) 263, 297, 320, 1276
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 6 cut(s) 30, 291, 672, 816, 1155, 1166
Cac8I GCNNGC 4 cut(s) 454, 542, 697, 1059
CfoI GCGC 3 cut(s) 1063, 1138, 1361
Cfr13I GGNCC 4 cut(s) 144, 184, 680, 823
Csp6I GTAC 6 cut(s) 35, 244, 295, 382, 470, 1069
CviQI GTAC 6 cut(s) 35, 244, 295, 382, 470, 1069
DdeI CTNAG 3 cut(s) 47, 743, 1410
DinI GGCGCC 1 cut(s) 1137
EaeI YGGCCR 2 cut(s) 447, 1188
EagI CGGCCG 1 cut(s) 1188
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EclXI CGGCCG 1 cut(s) 1188
Eco105I TACGTA 1 cut(s) 1374
Eco130I CCWWGG 1 cut(s) 819
Eco147I AGGCCT 1 cut(s) 52
Eco32I GATATC 1 cut(s) 1185
Eco47I GGWCC 4 cut(s) 144, 184, 680, 823
Eco52I CGGCCG 1 cut(s) 1188
Eco57I CTGAAG 2 cut(s) 1110, 1437
Eco88I CYCGRG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 300, 1131, 1284
EcoRV GATATC 1 cut(s) 1185
EcoT14I CCWWGG 1 cut(s) 819
EgeI GGCGCC 1 cut(s) 1137
EheI GGCGCC 1 cut(s) 1137
ErhI CCWWGG 1 cut(s) 819
FaqI GGGAC 3 cut(s) 693, 809, 1258
FauI CCCGC 1 cut(s) 393
FauNDI CATATG 2 cut(s) 561, 686
FbaI TGATCA 2 cut(s) 669, 1314
Fnu4HI GCNGC 7 cut(s) 450, 582, 693, 798, 996, 1161, 1164
FokI GGATG 4 cut(s) 250, 284, 327, 1283
Fsp4HI GCNGC 7 cut(s) 450, 582, 693, 798, 996, 1161, 1164
FspBI CTAG 7 cut(s) 131, 272, 290, 489, 872, 1058, 1115
GlaI GCGC 3 cut(s) 1062, 1137, 1360
GluI GCNGC 7 cut(s) 450, 582, 693, 798, 996, 1161, 1164
GsaI CCCAGC 2 cut(s) 797, 1245
HaeII RGCGCY 2 cut(s) 1064, 1139
HaeIII GGCC 3 cut(s) 52, 449, 1190
HapII CCGG 5 cut(s) 147, 407, 427, 1191, 1268
HhaI GCGC 3 cut(s) 1063, 1138, 1361
Hin1I GRCGYC 1 cut(s) 1136
Hin6I GCGC 3 cut(s) 1061, 1136, 1359
HinP1I GCGC 3 cut(s) 1061, 1136, 1359
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 6 cut(s) 927, 933, 969, 1018, 1384, 1412
HpaII CCGG 5 cut(s) 147, 407, 427, 1191, 1268
HphI GGTGA 4 cut(s) 606, 895, 1309, 1402
Hpy166II GTNNAC 4 cut(s) 98, 295, 423, 816
Hpy188III TCNNGA 2 cut(s) 572, 1174
Hpy8I GTNNAC 4 cut(s) 98, 295, 423, 816
Hpy99I CGWCG 2 cut(s) 95, 965
HpyAV CCTTC 3 cut(s) 923, 1085, 1412
HpyCH4III ACNGT 5 cut(s) 25, 95, 286, 640, 1170
HpyCH4IV ACGT 1 cut(s) 1373
HpyCH4V TGCA 4 cut(s) 4, 107, 456, 1166
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 590, 1001, 1152, 1187
HpyF3I CTNAG 3 cut(s) 47, 743, 1410
HpySE526I ACGT 1 cut(s) 1373
Hsp92I GRCGYC 1 cut(s) 1136
HspAI GCGC 3 cut(s) 1061, 1136, 1359
KasI GGCGCC 1 cut(s) 1135
KflI GGGWCCC 1 cut(s) 680
KpnI GGTACC 2 cut(s) 385, 473
Ksp22I TGATCA 2 cut(s) 669, 1314
LmnI GCTCC 7 cut(s) 134, 179, 228, 260, 1152, 1406, 1432
Lsp1109I GCAGC 6 cut(s) 593, 704, 809, 1007, 1147, 1150
LweI GCATC 1 cut(s) 272
MaeI CTAG 7 cut(s) 131, 272, 290, 489, 872, 1058, 1115
MaeII ACGT 1 cut(s) 1373
MaeIII GTNAC 4 cut(s) 62, 280, 612, 883
MbiI CCGCTC 1 cut(s) 182
MboII GAAGA 3 cut(s) 472, 611, 665
MflI RGATCY 3 cut(s) 153, 403, 1253
MhlI GDGCHC 5 cut(s) 139, 1113, 1157, 1208, 1230
MluCI AATT 7 cut(s) 108, 333, 566, 708, 977, 1195, 1276
Mly113I GGCGCC 1 cut(s) 1136
MlyI GAGTC 1 cut(s) 1421
MmeI TCCRAC 3 cut(s) 181, 362, 1281
MnlI CCTC 8 cut(s) 42, 63, 589, 621, 782, 811, 1143, 1220
MseI TTAA 2 cut(s) 237, 836
MslI CAYNNNNRTG 1 cut(s) 809
MspI CCGG 5 cut(s) 147, 407, 427, 1191, 1268
MspR9I CCNGG 5 cut(s) 302, 427, 1133, 1192, 1286
Mva1269I GAATGC 3 cut(s) 173, 845, 1094
MvaI CCWGG 3 cut(s) 302, 1133, 1286
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 5 cut(s) 179, 590, 1001, 1152, 1187
NarI GGCGCC 1 cut(s) 1136
NciI CCSGG 2 cut(s) 427, 1192
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 2 cut(s) 561, 686
NheI GCTAGC 1 cut(s) 1057
NmuCI GTSAC 4 cut(s) 62, 280, 612, 883
PceI AGGCCT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 3 cut(s) 849, 969, 1379
PctI GAATGC 3 cut(s) 173, 845, 1094
PfeI GAWTC 5 cut(s) 927, 933, 969, 1018, 1384
PflFI GACNNNGTC 1 cut(s) 25
PflMI CCANNNNNTGG 2 cut(s) 225, 624
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 7 cut(s) 451, 583, 694, 799, 997, 1162, 1165
PleI GAGTC 1 cut(s) 1420
PluTI GGCGCC 1 cut(s) 1139
PpsI GAGTC 1 cut(s) 1420
Ppu21I YACGTR 1 cut(s) 1374
PpuMI RGGWCCY 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 3 cut(s) 300, 1131, 1284
PspFI CCCAGC 2 cut(s) 793, 1241
PspGI CCWGG 3 cut(s) 300, 1131, 1284
PspPI GGNCC 4 cut(s) 144, 184, 680, 823
PspPPI RGGWCCY 1 cut(s) 680
PsuI RGATCY 3 cut(s) 153, 403, 1253
PsyI GACNNNGTC 1 cut(s) 25
RsaI GTAC 6 cut(s) 36, 245, 296, 383, 471, 1070
RsaNI GTAC 6 cut(s) 35, 244, 295, 382, 470, 1069
RseI CAYNNNNRTG 1 cut(s) 809
SaqAI TTAA 2 cut(s) 237, 836
SatI GCNGC 7 cut(s) 450, 582, 693, 798, 996, 1161, 1164
Sau96I GGNCC 4 cut(s) 144, 184, 680, 823
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 1421
ScrFI CCNGG 5 cut(s) 302, 427, 1133, 1192, 1286
SduI GDGCHC 5 cut(s) 139, 1113, 1157, 1208, 1230
SfaNI GCATC 1 cut(s) 272
SfcI CTRYAG 2 cut(s) 342, 1126
SfoI GGCGCC 1 cut(s) 1137
SinI GGWCC 4 cut(s) 144, 184, 680, 823
SmiMI CAYNNNNRTG 1 cut(s) 809
SmlI CTYRAG 2 cut(s) 608, 1229
SmoI CTYRAG 2 cut(s) 608, 1229
SnaBI TACGTA 1 cut(s) 1374
Sse9I AATT 7 cut(s) 108, 333, 566, 708, 977, 1195, 1276
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 3 cut(s) 182, 386, 450
SspDI GGCGCC 1 cut(s) 1135
SspMI CTAG 7 cut(s) 131, 272, 290, 489, 872, 1058, 1115
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 5 cut(s) 300, 425, 1131, 1190, 1284
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 5 cut(s) 25, 95, 286, 640, 1170
TaiI ACGT 1 cut(s) 1376
TaqI TCGA 1 cut(s) 394
TaqII GACCGA 1 cut(s) 870
TasI AATT 7 cut(s) 108, 333, 566, 708, 977, 1195, 1276
TatI WGTACW 3 cut(s) 34, 294, 1068
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 5 cut(s) 927, 933, 969, 1018, 1384
Tru1I TTAA 2 cut(s) 237, 836
Tru9I TTAA 2 cut(s) 237, 836
TscAI CASTG 6 cut(s) 30, 291, 672, 816, 1162, 1173
TseFI GTSAC 4 cut(s) 62, 280, 612, 883
TseI GCWGC 6 cut(s) 581, 692, 797, 995, 1160, 1163
Tsp45I GTSAC 4 cut(s) 62, 280, 612, 883
TspDTI ATGAA 8 cut(s) 525, 578, 632, 776, 853, 1067, 1087, 1209
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 6 cut(s) 30, 291, 672, 816, 1162, 1173
Tth111I GACNNNGTC 1 cut(s) 25
Van91I CCANNNNNTGG 2 cut(s) 225, 624
VpaK11BI GGWCC 4 cut(s) 144, 184, 680, 823
XapI RAATTY 2 cut(s) 333, 566
XspI CTAG 7 cut(s) 131, 272, 290, 489, 872, 1058, 1115
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.