Rh4BG176300

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0021398
Physical Location & Seq
Forward (+)
31299396 .. 31300444
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG176300.1

Sequence Viewer

Length: 414 bp
ATGCAACTGTGGCAGTGTACACTAGCTATGCTGACACTTTCAACAATTCCAGGCCACTATGTGCTTTTCCGGGAGCTTAGCCTTAATGGCTCAACTCCAATCCCACCCTCAGTGTTTGAGGACTACTGGTTTGCCATTGAGGAGTCACTCAACAATGTGTACCGCCAAGGCAGAGTCTCCGACAAATCGATCAGGCCGCTGGAGATCAAGATTGTTCAGGCTGGGACTTTTGACAAGCTCATCGACTATGCCATAAGCTTGGGAGCTTCCATTAACCAATACAAGACCCCAAGGTGTGTGAAGTTTACACTCATTGTTGAGCTCTTGAACTCTAGGGTGGTCTCAAACTACTTCAGTCCTAAGTACCCCAAGTGGGCCCCTGGCCACAAGCAATGGTGCAAAAACTATGATTGA

Protein Analysis

137

Amino Acids

15.81

Weight (kDa)

8.99

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3_C PF23572 7 - 102 9.2e-27 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021398)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0412851
rosa_multiflora Rmu_sc0003022.1_g000006
rosa_samantha Rh4BG176300 Rh4CG187000 Rh4DG173000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 163, 197
AcoI YGGCCR 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 337
AdeI CACNNNGTG 1 cut(s) 61
AfaI GTAC 3 cut(s) 19, 161, 365
AfiI CCNNNNNNNGG 1 cut(s) 373
AgsI TTSAA 2 cut(s) 42, 328
AjnI CCWGG 2 cut(s) 49, 379
AluBI AGCT 6 cut(s) 26, 76, 238, 258, 266, 322
AluI AGCT 6 cut(s) 26, 76, 238, 258, 266, 322
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 181, 346
AoxI GGCC 4 cut(s) 52, 194, 375, 382
ApaI GGGCCC 1 cut(s) 379
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspS9I GGNCC 2 cut(s) 375, 376
AsuC2I CCSGG 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 379
BalI TGGCCA 1 cut(s) 384
BanII GRGCYC 2 cut(s) 324, 379
Bbv12I GWGCWC 1 cut(s) 324
BciT130I CCWGG 2 cut(s) 51, 381
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 181, 346
BfaI CTAG 2 cut(s) 23, 333
BglI GCCNNNNNGGC 1 cut(s) 87
BisI GCNGC 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 51, 71, 381
BmgT120I GGNCC 2 cut(s) 375, 376
BmiI GGNNCC 2 cut(s) 377, 378
BmrFI CCNGG 3 cut(s) 51, 71, 381
BpmI CTGGAG 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 166, 290, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 398
BseBI CCWGG 2 cut(s) 51, 381
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 166, 290, 379
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 221
BshFI GGCC 4 cut(s) 54, 196, 377, 384
BshVI ATCGAT 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 181, 346
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 4 cut(s) 54, 196, 377, 384
Bso31I GGTCTC 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 375
Bsp1286I GDGCHC 2 cut(s) 324, 379
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 2 cut(s) 189, 204
Bsp1720I GCTNAGC 1 cut(s) 77
BspACI CCGC 2 cut(s) 163, 197
BspANI GGCC 4 cut(s) 54, 196, 377, 384
BspCNI CTCAG 1 cut(s) 122
BspDI ATCGAT 1 cut(s) 188
BspLI GGNNCC 2 cut(s) 377, 378
BspTNI GGTCTC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 398
BsrGI TGTACA 1 cut(s) 17
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 166, 290, 379
BssMI GATC 2 cut(s) 189, 204
BssT1I CCWWGG 2 cut(s) 166, 290
Bst2UI CCWGG 2 cut(s) 51, 381
Bst4CI ACNGT 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 17
BstDEI CTNAG 3 cut(s) 77, 109, 360
BstKTI GATC 2 cut(s) 192, 207
BstMAI GTCTC 2 cut(s) 181, 346
BstMBI GATC 2 cut(s) 189, 204
BstMWI GCNNNNNNNGC 2 cut(s) 10, 87
BstNI CCWGG 2 cut(s) 51, 381
BstSCI CCNGG 3 cut(s) 49, 69, 379
BstSLI GKGCMC 1 cut(s) 379
BstXI CCANNNNNNTGG 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 188
BsuRI GGCC 4 cut(s) 54, 196, 377, 384
BsuTUI ATCGAT 1 cut(s) 188
BtsI GCAGTG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 20, 117
Cfr13I GGNCC 2 cut(s) 375, 376
ClaI ATCGAT 1 cut(s) 188
Csp6I GTAC 3 cut(s) 18, 160, 364
CviQI GTAC 3 cut(s) 18, 160, 364
DdeI CTNAG 3 cut(s) 77, 109, 360
DpnI GATC 2 cut(s) 191, 206
DpnII GATC 2 cut(s) 189, 204
DraIII CACNNNGTG 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 166, 290
Eco24I GRGCYC 2 cut(s) 324, 379
Eco31I GGTCTC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 49, 379
EcoT14I CCWWGG 2 cut(s) 166, 290
EcoT38I GRGCYC 2 cut(s) 324, 379
ErhI CCWWGG 2 cut(s) 166, 290
FaiI YATR 5 cut(s) 29, 60, 249, 254, 408
FaqI GGGAC 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 197
FriOI GRGCYC 2 cut(s) 324, 379
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 2 cut(s) 23, 333
GluI GCNGC 1 cut(s) 197
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 4 cut(s) 54, 196, 377, 384
HapII CCGG 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 2 cut(s) 143, 174
HpaII CCGG 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 18, 20, 160, 306
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 2 cut(s) 208, 325
Hpy8I GTNNAC 4 cut(s) 18, 20, 160, 306
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 4, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 87
HpyF3I CTNAG 3 cut(s) 77, 109, 360
Kzo9I GATC 2 cut(s) 189, 204
LmnI GCTCC 2 cut(s) 73, 263
MaeI CTAG 2 cut(s) 23, 333
MaeIII GTNAC 1 cut(s) 144
MalI GATC 2 cut(s) 191, 206
MboI GATC 2 cut(s) 189, 204
MhlI GDGCHC 2 cut(s) 324, 379
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 2 cut(s) 152, 183
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 3 cut(s) 112, 118, 133
Mox20I TGGCCA 1 cut(s) 384
MscI TGGCCA 1 cut(s) 384
MseI TTAA 2 cut(s) 84, 273
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 3 cut(s) 51, 71, 381
MvaI CCWGG 2 cut(s) 51, 381
MwoI GCNNNNNNNGC 2 cut(s) 10, 87
NciI CCSGG 1 cut(s) 71
NdeII GATC 2 cut(s) 189, 204
NlaIV GGNNCC 2 cut(s) 377, 378
NmuCI GTSAC 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 69
PkrI GCNGC 1 cut(s) 198
PleI GAGTC 2 cut(s) 151, 182
PpsI GAGTC 2 cut(s) 151, 182
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 49, 379
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 2 cut(s) 49, 379
PspN4I GGNNCC 2 cut(s) 377, 378
PspOMI GGGCCC 1 cut(s) 375
PspPI GGNCC 2 cut(s) 375, 376
RsaI GTAC 3 cut(s) 19, 161, 365
RsaNI GTAC 3 cut(s) 18, 160, 364
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 84, 273
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 2 cut(s) 189, 204
Sau96I GGNCC 2 cut(s) 375, 376
SchI GAGTC 2 cut(s) 152, 183
ScrFI CCNGG 3 cut(s) 51, 71, 381
SduI GDGCHC 2 cut(s) 324, 379
SetI ASST 7 cut(s) 28, 78, 240, 260, 268, 296, 324
Sse9I AATT 1 cut(s) 45
SsiI CCGC 2 cut(s) 163, 197
SspMI CTAG 2 cut(s) 23, 333
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 3 cut(s) 49, 69, 379
StyI CCWWGG 2 cut(s) 166, 290
TaaI ACNGT 1 cut(s) 9
TaqI TCGA 2 cut(s) 188, 243
TasI AATT 1 cut(s) 45
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 84, 273
Tru9I TTAA 2 cut(s) 84, 273
TscAI CASTG 2 cut(s) 20, 117
TseFI GTSAC 1 cut(s) 144
Tsp45I GTSAC 1 cut(s) 144
TspRI CASTG 2 cut(s) 20, 117
XspI CTAG 2 cut(s) 23, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.