Rh4CG013300

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018528
Physical Location & Seq
Forward (+)
2280990 .. 2282249
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG013300.1

Sequence Viewer

Length: 540 bp
ATGGCCTCGAAACGGACCCGTATTTTTCAATCATCGAGTCACGGTAATATCAGCATATTCAACCAAATGAATCGTTTAGAGTCGCCTGTGGCTTTGTGGCACCAGAAGCACAAGCAAGCTCCATTAGTCATAGACGACATGCTGCCTCGAGTACAACAACCAGCCACCACGAAACCAAGTGTCATACAACCACGCCGGAGTATCTTTCAGTTGGCTTCACCGCCGGGGTCACCGGTTCGGGAGAGGCAACGTAAGGAAGGGTCTAATAAGGATTTTCTCAAAGCCTGTTCCTTGTGCAAGAAGGATCTCCCAGAAAAGGACCTCTACATGTATGGTGACCTAGCCGCATTCTGCTCCATTGAATGCCGAGATCATCAGATGACTTTGGACGGGTTTCACAAAAAAACTGTAGTAGATTGTAATAATGGAATGCTTAAGGAAGCTATAGCCCTAGCAAAGGTACATGTACTGTACCTAGCACGGAAGAAGAAGATACGTGATGCCATATTAGCCCAAGAAGAAGCTACGGCAATGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

179

Amino Acids

20.29

Weight (kDa)

9.62

Isoelectric Point (pI)

65.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 83 - 130 1.4e-17 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018528)

Species Orthologous Gene IDs
prunus_persica Prupe.1G018100_v2.0.a1
rosa_laevigata RLG00000010165
rosa_multiflora Rmu_sc0000472.1_g000004
rosa_roxburghii Rroxscaffold_5G00333890
rosa_rugosa Rorug03G0306700
rosa_samantha Rh4AG011100 Rh4BG008700 Rh4CG013300 Rh4DG009300
rosa_wichuraiana Rw4G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 99
AciI CCGC 2 cut(s) 221, 345
AclWI GGATC 1 cut(s) 312
AfaI GTAC 4 cut(s) 153, 462, 468, 473
AfiI CCNNNNNNNGG 3 cut(s) 12, 316, 457
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 2 cut(s) 327, 463
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 3 cut(s) 29, 61, 362
AluBI AGCT 3 cut(s) 119, 443, 524
AluI AGCT 3 cut(s) 119, 443, 524
AlwI GGATC 1 cut(s) 312
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 142
AsiGI ACCGGT 1 cut(s) 232
AspS9I GGNCC 2 cut(s) 15, 319
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 210, 222, 347
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 2 cut(s) 15, 319
BanI GGYRCC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 129
BcnI CCSGG 1 cut(s) 225
BfaI CTAG 3 cut(s) 341, 452, 476
BfmI CTRYAG 2 cut(s) 408, 444
BfrI CTTAAG 1 cut(s) 434
BisI GCNGC 2 cut(s) 143, 345
BlsI GCNGC 2 cut(s) 144, 346
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 2 cut(s) 15, 319
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 15, 319
BmiI GGNNCC 2 cut(s) 17, 101
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 490
BpuMI CCSGG 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 316, 457
Bse118I RCCGGY 1 cut(s) 232
Bse3DI GCAATG 1 cut(s) 537
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 3 cut(s) 12, 316, 457
BseMI GCAATG 1 cut(s) 537
BseXI GCAGC 1 cut(s) 129
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 232
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 3 cut(s) 196, 224, 233
BslI CCNNNNNNNGG 3 cut(s) 12, 316, 457
BsmI GAATGC 3 cut(s) 347, 368, 435
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 147
Bsp143I GATC 2 cut(s) 304, 370
BspACI CCGC 2 cut(s) 221, 345
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 17, 101
BspPI GGATC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 99
BspTI CTTAAG 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 537
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 2 cut(s) 304, 370
Bst4CI ACNGT 3 cut(s) 44, 409, 471
BstAFI CTTAAG 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 117
BstEII GGTNACC 2 cut(s) 228, 335
BstENI CCTNNNNNAGG 1 cut(s) 455
BstKTI GATC 2 cut(s) 307, 373
BstMBI GATC 2 cut(s) 304, 370
BstMWI GCNNNNNNNGC 2 cut(s) 106, 509
BstNSI RCATGY 3 cut(s) 142, 331, 467
BstPI GGTNACC 2 cut(s) 228, 335
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 2 cut(s) 408, 444
BstV1I GCAGC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 15, 319
Csp6I GTAC 4 cut(s) 152, 461, 467, 472
CspAI ACCGGT 1 cut(s) 232
CviAII CATG 3 cut(s) 139, 328, 464
CviQI GTAC 4 cut(s) 152, 461, 467, 472
DpnI GATC 2 cut(s) 306, 372
DpnII GATC 2 cut(s) 304, 370
Eco47I GGWCC 2 cut(s) 15, 319
Eco88I CYCGRG 1 cut(s) 147
Eco91I GGTNACC 2 cut(s) 228, 335
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 2 cut(s) 228, 335
FaeI CATG 3 cut(s) 142, 331, 467
FatI CATG 3 cut(s) 138, 327, 463
Fnu4HI GCNGC 2 cut(s) 143, 345
Fsp4HI GCNGC 2 cut(s) 143, 345
FspBI CTAG 3 cut(s) 341, 452, 476
GluI GCNGC 2 cut(s) 143, 345
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 196, 224, 233
Hin1II CATG 3 cut(s) 142, 331, 467
HinfI GANTC 3 cut(s) 37, 70, 80
HpaII CCGG 3 cut(s) 196, 224, 233
HphI GGTGA 3 cut(s) 210, 222, 347
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 251, 295
HpyCH4III ACNGT 3 cut(s) 44, 409, 471
HpyCH4IV ACGT 2 cut(s) 250, 496
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 509
HpySE526I ACGT 2 cut(s) 250, 496
Hsp92II CATG 3 cut(s) 142, 331, 467
Kzo9I GATC 2 cut(s) 304, 370
LmnI GCTCC 2 cut(s) 124, 359
LpnPI CCDG 8 cut(s) 99, 116, 174, 209, 237, 246, 298, 324
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 1 cut(s) 490
MaeI CTAG 3 cut(s) 341, 452, 476
MaeII ACGT 2 cut(s) 250, 496
MaeIII GTNAC 3 cut(s) 38, 228, 335
MalI GATC 2 cut(s) 306, 372
MboI GATC 2 cut(s) 304, 370
MboII GAAGA 4 cut(s) 496, 499, 502, 530
MflI RGATCY 1 cut(s) 304
MlyI GAGTC 2 cut(s) 46, 89
MnlI CCTC 4 cut(s) 16, 156, 237, 332
MseI TTAA 1 cut(s) 435
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 3 cut(s) 196, 224, 233
MspR9I CCNGG 1 cut(s) 225
Mva1269I GAATGC 3 cut(s) 347, 368, 435
MwoI GCNNNNNNNGC 2 cut(s) 106, 509
NciI CCSGG 1 cut(s) 225
NdeII GATC 2 cut(s) 304, 370
NlaIII CATG 3 cut(s) 142, 331, 467
NlaIV GGNNCC 2 cut(s) 17, 101
NmeAIII GCCGAG 1 cut(s) 392
NmuCI GTSAC 3 cut(s) 38, 228, 335
NspI RCATGY 3 cut(s) 142, 331, 467
PaeR7I CTCGAG 1 cut(s) 147
PciI ACATGT 2 cut(s) 327, 463
PctI GAATGC 3 cut(s) 347, 368, 435
PfeI GAWTC 1 cut(s) 70
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 2 cut(s) 144, 346
PleI GAGTC 2 cut(s) 45, 88
PpsI GAGTC 2 cut(s) 45, 88
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 2 cut(s) 327, 463
Psp5II RGGWCCY 1 cut(s) 319
PspEI GGTNACC 2 cut(s) 228, 335
PspN4I GGNNCC 2 cut(s) 17, 101
PspPI GGNCC 2 cut(s) 15, 319
PspPPI RGGWCCY 1 cut(s) 319
PspXI VCTCGAGB 1 cut(s) 147
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 4 cut(s) 153, 462, 468, 473
RsaNI GTAC 4 cut(s) 152, 461, 467, 472
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 2 cut(s) 143, 345
Sau3AI GATC 2 cut(s) 304, 370
Sau96I GGNCC 2 cut(s) 15, 319
SchI GAGTC 2 cut(s) 46, 89
ScrFI CCNGG 1 cut(s) 225
SetI ASST 9 cut(s) 121, 253, 324, 342, 445, 462, 477, 499, 526
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 2 cut(s) 408, 444
Sfr274I CTCGAG 1 cut(s) 147
SinI GGWCC 2 cut(s) 15, 319
SlaI CTCGAG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 147, 434
SmoI CTYRAG 2 cut(s) 147, 434
SsiI CCGC 2 cut(s) 221, 345
SspMI CTAG 3 cut(s) 341, 452, 476
StyD4I CCNGG 1 cut(s) 223
TaaI ACNGT 3 cut(s) 44, 409, 471
TaiI ACGT 2 cut(s) 253, 499
TaqI TCGA 3 cut(s) 8, 35, 148
TatI WGTACW 2 cut(s) 151, 466
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TseFI GTSAC 3 cut(s) 38, 228, 335
TseI GCWGC 1 cut(s) 142
Tsp45I GTSAC 3 cut(s) 38, 228, 335
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 2 cut(s) 28, 496
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 15, 319
XagI CCTNNNNNAGG 1 cut(s) 455
XceI RCATGY 3 cut(s) 142, 331, 467
XhoI CTCGAG 1 cut(s) 147
XspI CTAG 3 cut(s) 341, 452, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.