Rh4CG134500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018920
Physical Location & Seq
Reverse (-)
26520142 .. 26537597
17456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG134500.1

Sequence Viewer

Length: 369 bp
ATGAGAACAAGGAGTTCAATTCCATGGAGAATTAAGTCGAAGAAGAGAAAGCTAAAGCTCTCTTTTGGTAGAAACTCCGAACTGGTTGCCAAATCGTCCATAGAAAGGAGTCTGATGGAGCTGGGAAGGATCATCCCCGGGTGCATTGTTGACAGCAATAACCCAGAAACCTTGTTCCAAGGAATTGCTAATTACATCTATCTCCTGGAAACCAAAACTGCAATAAATGAACCAAATTCAGAAATATTAGTTAGTACTGTTGAAAAAATTCTTCTAGGTAGTCAGGCTGTTGTGGTTAGGGGTTTTAGTGTGGTGGCCCTGGCCAGCTCTAGCAAGCTTAACCCTATCTTAAGCTCCCTTCATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.43

Weight (kDa)

10.08

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018920)

Species Orthologous Gene IDs
prunus_persica Prupe.1G097700_v2.0.a1
pyrus_communis pycom16g23040
rosa_chinensis RchiOBHm_Chr4g0404971
rosa_roxburghii Rroxscaffold_5G00349100
rosa_rugosa Rorug04G0050600
rosa_samantha Rh4AG126300 Rh4BG120500 Rh4CG134500 Rh4DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 137
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 2 cut(s) 235, 267
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 105
AflII CTTAAG 1 cut(s) 349
AgsI TTSAA 2 cut(s) 18, 263
AjnI CCWGG 2 cut(s) 204, 318
AluBI AGCT 6 cut(s) 52, 58, 121, 327, 337, 354
AluI AGCT 6 cut(s) 52, 58, 121, 327, 337, 354
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 315, 321
ApoI RAATTY 2 cut(s) 235, 267
Asp700I GAANNNNTTC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 2 cut(s) 138, 139
AvaI CYCGRG 1 cut(s) 137
BalI TGGCCA 1 cut(s) 323
BccI CCATC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 2 cut(s) 206, 320
BcnI CCSGG 2 cut(s) 138, 139
BfaI CTAG 2 cut(s) 275, 330
BfrI CTTAAG 1 cut(s) 349
BmcAI AGTACT 1 cut(s) 256
Bme1390I CCNGG 4 cut(s) 138, 139, 206, 320
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 316
BmrFI CCNGG 4 cut(s) 138, 139, 206, 320
BpuMI CCSGG 2 cut(s) 138, 139
BsaJI CCNNGG 5 cut(s) 23, 136, 137, 178, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 87
BseBI CCWGG 2 cut(s) 206, 320
BseDI CCNNGG 5 cut(s) 23, 136, 137, 178, 318
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 87
BseYI CCCAGC 1 cut(s) 121
BshFI GGCC 2 cut(s) 317, 323
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 105
BsnI GGCC 2 cut(s) 317, 323
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 23
BspANI GGCC 2 cut(s) 317, 323
BspPI GGATC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 349
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 5 cut(s) 23, 136, 137, 178, 318
BssMI GATC 1 cut(s) 129
BssT1I CCWWGG 2 cut(s) 23, 178
Bst2UI CCWGG 2 cut(s) 206, 320
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 325, 335
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstNI CCWGG 2 cut(s) 206, 320
BstSCI CCNGG 4 cut(s) 136, 137, 204, 318
BsuRI GGCC 2 cut(s) 317, 323
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 325, 335
Cfr13I GGNCC 1 cut(s) 316
Cfr9I CCCGGG 1 cut(s) 137
Csp6I GTAC 1 cut(s) 255
CviAII CATG 2 cut(s) 24, 362
CviJI RGCY 9 cut(s) 52, 58, 121, 287, 317, 323, 327, 337, 354
CviKI_1 RGCY 9 cut(s) 52, 58, 121, 287, 317, 323, 327, 337, 354
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 23, 178
Eco88I CYCGRG 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 204, 318
EcoT14I CCWWGG 2 cut(s) 23, 178
ErhI CCWWGG 2 cut(s) 23, 178
FaeI CATG 2 cut(s) 27, 365
FaiI YATR 3 cut(s) 25, 101, 363
FatI CATG 2 cut(s) 23, 361
FokI GGATG 1 cut(s) 119
FspBI CTAG 2 cut(s) 275, 330
GsaI CCCAGC 1 cut(s) 125
HaeIII GGCC 2 cut(s) 317, 323
HapII CCGG 1 cut(s) 138
Hin1II CATG 2 cut(s) 27, 365
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 3 cut(s) 79, 114, 241
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 120, 368
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 144, 221
HpyF3I CTNAG 1 cut(s) 366
Hsp92II CATG 2 cut(s) 27, 365
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 118, 359
MaeI CTAG 2 cut(s) 275, 330
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 3 cut(s) 52, 55, 263
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 6 cut(s) 18, 30, 183, 190, 235, 267
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 1 cut(s) 118
Mox20I TGGCCA 1 cut(s) 323
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 323
MseI TTAA 3 cut(s) 33, 339, 350
Msp20I TGGCCA 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 4 cut(s) 138, 139, 206, 320
MvaI CCWGG 2 cut(s) 206, 320
NciI CCSGG 2 cut(s) 138, 139
NcoI CCATGG 1 cut(s) 23
NdeII GATC 1 cut(s) 129
NlaIII CATG 2 cut(s) 27, 365
PdmI GAANNNNTTC 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 204
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 204, 318
PspFI CCCAGC 1 cut(s) 121
PspGI CCWGG 2 cut(s) 204, 318
PspPI GGNCC 1 cut(s) 316
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SaqAI TTAA 3 cut(s) 33, 339, 350
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 4 cut(s) 138, 139, 206, 320
SetI ASST 8 cut(s) 54, 60, 123, 173, 280, 329, 339, 356
SmaI CCCGGG 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 6 cut(s) 18, 30, 183, 190, 235, 267
SspI AATATT 1 cut(s) 246
SspMI CTAG 2 cut(s) 275, 330
StyD4I CCNGG 4 cut(s) 136, 137, 204, 318
StyI CCWWGG 2 cut(s) 23, 178
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 38
TasI AATT 6 cut(s) 18, 30, 183, 190, 235, 267
TatI WGTACW 1 cut(s) 254
Tru1I TTAA 3 cut(s) 33, 339, 350
Tru9I TTAA 3 cut(s) 33, 339, 350
TspDTI ATGAA 2 cut(s) 243, 350
TspMI CCCGGG 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 349
XapI RAATTY 2 cut(s) 235, 267
XmaI CCCGGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 2 cut(s) 275, 330
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.