Rh4CG161600

Galactolipase DONGLE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016585
Physical Location & Seq
Forward (+)
35466823 .. 35468492
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG161600.1

Sequence Viewer

Length: 1416 bp
ATGGCTTCCATGAACATTCACATGCCCAACACAAACTTAAACCATGTTGCATTTTCCGAAACCACTCGGCCGCTGCGGTCGGTTTGTATCGGGCAGGTTTGTCTGCCCAAACGAACCAAATTAGCAACAGCTTCTCCTACAAAACAAACGGTCGTCGCATCTCCGGTGGCCATTGAAGTAGCAGCTAGAGCTAGCTCTGGTTCTAGTACTAGTACTACTTCTTTGTCTCATTCGTGGAGAGAGATTCAAGGCTCCGACAACTGGGAAAATCTAGTAGACCCATTGCACCCTCTTCTCCGGCAAGAGATAATCCGGTACGGCGACTTTGTAAAGGCGTGTTACAACGCTTTCGACCTGGACCCCAATTCGAAACGCTACCTCAACTGCAAGTACAGCAAGAGAAACATGCTGAGAGAGATTGGGATGGAGAGCAGCGGCTATGAAGTCACCAAGTACATCTATGCTACCCCCGACATCAACATTCCCATCCAAAACGGCGAGTCGTGCGGACGCTGGATCGGATATGTGGCCGTTGCCACTGACGAAGCGGTGAAGCGGCTAGGAAGGAGAGATATTATCATCACCTTCCGAGGCACGGTTACAAACCCTGAGTGGGTGGCAAACCTAATGAGCTCCCTCACTCCGGCAAGGCTTGATCCTCATAATGAACGGCCTGACGTGAAAGTAGAGTCTGGGTTTCTCAGCTTGTACACTTCCGATGAAAGCGACAGTAGGTTCGGGCTAGGAAGTTGCCGGGAACAGCTTCTTTCCGAAGTGTCGAGGCTACTAAACAAGTACAAAGGCGAGGAGTTAAGCATAACCTTAGCAGGACACAGCATGGGAAGTTCTCTGGCTCTTCTTCTGGCTTACGACATCACGGAGCTAGGGTTGAATCGTGTCAATTCGAACATGAAAACACCCGTCACCGTGTTTTCCTTCGGAGGCCCCAGAGTTGGGAATCCCGGGTTCAAGATGCGATGTGAAGAGTTGGGTGTCAAAGTGTTGAGGATAGTAAACGTGAACGATCCGATCACAAAGATGCCCGGAATGGTTTTCAATCATGAGAATTTTAGGGTTTTGGGCAGTGAGCGATTGTCATTTGAGTTCCCTTGGAGTTGCTCCTGTTATGCACATGTTGGTGTTGAATTGGTGCTTGATTTCTTCAACATGCAAAACCCTTCCTGTGTTCATGATCTGGGGACTTATATCAGCTTGCTCAAATGCCCCAAGAGACCATCAATGGAAATTCAAAGAGATAATCAAGGTGGGGATTTTGTGAACAAGGCAAAAGTATTGCTCTCGAATATAATTGGTGCGCAAAATTTGAACCTGCAGCTACCAATCCCATGGAGAAGTAGTGGTACTACTGGTGAAAGCAATATGGTGAATCTGCAGGTTCAATCACAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005811 GO:0006082 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006950 GO:0006952 GO:0006996 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008970 GO:0009056 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009653 GO:0009657 GO:0009668 GO:0009694 GO:0009695 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010022 GO:0010027 GO:0010073 GO:0010150 GO:0010582 GO:0010817 GO:0016042 GO:0016043 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019433 GO:0019752 GO:0022414 GO:0030308 GO:0032501 GO:0032502 GO:0032787 GO:0040008 GO:0042445 GO:0042446 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045926 GO:0046394 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047714 GO:0048366 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048507 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051704 GO:0051707 GO:0052689 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0072330 GO:0090567 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0098542 GO:0099402 GO:1901575 GO:1901576 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

471

Amino Acids

52.38

Weight (kDa)

8.55

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 194 - 351 3e-36 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016585)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05800
fragaria_vesca FvH4_4g11680
malus_domestica MD04G1032700.v1.1
prunus_persica Prupe.1G181800_v2.0.a1
pyrus_communis pycom04g02700
rosa_chinensis RchiOBHm_Chr4g0408991
rosa_laevigata RLG00000008599
rosa_multiflora Rmu_sc0003685.1_g000001
rosa_roxburghii Rroxscaffold_5G00353440
rosa_rugosa Rorug04G0089000
rosa_samantha Rh4AG153200 Rh4BG151000 Rh4CG161600 Rh4DG146500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1317
Acc36I ACCTGC 3 cut(s) 85, 1338, 1384
AccI GTMKAC 1 cut(s) 276
AciI CCGC 6 cut(s) 71, 76, 435, 507, 548, 556
AclWI GGATC 3 cut(s) 524, 650, 1019
AcoI YGGCCR 3 cut(s) 68, 168, 528
AcsI RAATTY 3 cut(s) 1066, 1245, 1321
AfaI GTAC 8 cut(s) 208, 214, 317, 392, 455, 710, 797, 1363
AfiI CCNNNNNNNGG 6 cut(s) 261, 595, 613, 643, 953, 954
AflIII ACRYGT 1 cut(s) 1132
AhlI ACTAGT 1 cut(s) 209
AjiI CACGTC 1 cut(s) 679
AjnI CCWGG 1 cut(s) 354
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 2 cut(s) 231, 1225
AlwI GGATC 3 cut(s) 524, 650, 1019
Ama87I CYCGRG 1 cut(s) 962
AoxI GGCC 5 cut(s) 68, 168, 528, 671, 943
ApeKI GCWGC 4 cut(s) 73, 182, 432, 1333
ApoI RAATTY 3 cut(s) 1066, 1245, 1321
ArsI GACNNNNNNTTYG 2 cut(s) 719, 751
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 1318
AspS9I GGNCC 2 cut(s) 358, 944
AsuC2I CCSGG 4 cut(s) 755, 963, 964, 1044
AsuHPI GGTGA 6 cut(s) 439, 562, 574, 916, 1382, 1396
AsuII TTCGAA 2 cut(s) 368, 905
AsuNHI GCTAGC 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 962
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 170
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 1345, 1377
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 4 cut(s) 60, 194, 444, 1345
BccI CCATC 3 cut(s) 418, 494, 1243
BceAI ACGGC 4 cut(s) 334, 511, 515, 686
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 4 cut(s) 755, 963, 964, 1044
BcoDI GTCTC 2 cut(s) 231, 1225
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 8 cut(s) 186, 192, 204, 210, 272, 560, 743, 884
BfmI CTRYAG 2 cut(s) 1331, 1391
BfuAI ACCTGC 3 cut(s) 85, 1338, 1384
BisI GCNGC 7 cut(s) 71, 74, 183, 433, 436, 557, 1334
BlsI GCNGC 7 cut(s) 72, 75, 184, 434, 437, 558, 1335
BmcAI AGTACT 2 cut(s) 208, 214
Bme1390I CCNGG 5 cut(s) 356, 755, 963, 964, 1044
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 962
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 2 cut(s) 358, 944
BmiI GGNNCC 3 cut(s) 253, 360, 946
BmrFI CCNGG 5 cut(s) 356, 755, 963, 964, 1044
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 3 cut(s) 167, 963, 1029
BmtI GCTAGC 1 cut(s) 195
BmuI ACTGGG 1 cut(s) 271
Bpu10I CCTNAGC 1 cut(s) 823
Bpu14I TTCGAA 2 cut(s) 368, 905
BpuMI CCSGG 4 cut(s) 755, 963, 964, 1044
BsaI GGTCTC 1 cut(s) 1225
BsaJI CCNNGG 4 cut(s) 589, 962, 1109, 1346
BsaWI WCCGGW 2 cut(s) 163, 312
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 872, 902, 1342, 1372
Bsc4I CCNNNNNNNGG 6 cut(s) 261, 595, 613, 643, 953, 954
Bse1I ACTGG 2 cut(s) 266, 1372
Bse3DI GCAATG 1 cut(s) 281
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 589, 962, 1109, 1346
BseGI GGATG 2 cut(s) 429, 486
BseLI CCNNNNNNNGG 6 cut(s) 261, 595, 613, 643, 953, 954
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 3 cut(s) 401, 600, 715
BseNI ACTGG 2 cut(s) 266, 1372
BseRI GAGGAG 1 cut(s) 821
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 4 cut(s) 60, 194, 444, 1345
Bsh1285I CGRYCG 3 cut(s) 71, 80, 153
BshFI GGCC 5 cut(s) 70, 170, 530, 673, 945
BsiEI CGRYCG 3 cut(s) 71, 80, 153
BsiHKAI GWGCWC 1 cut(s) 635
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 7 cut(s) 164, 298, 313, 644, 754, 963, 1044
BslFI GGGAC 1 cut(s) 1213
BslI CCNNNNNNNGG 6 cut(s) 261, 595, 613, 643, 953, 954
BsmAI GTCTC 2 cut(s) 231, 1225
BsmFI GGGAC 1 cut(s) 1213
BsnI GGCC 5 cut(s) 70, 170, 530, 673, 945
Bso31I GGTCTC 1 cut(s) 1225
BsoBI CYCGRG 1 cut(s) 962
Bsp119I TTCGAA 2 cut(s) 368, 905
Bsp1286I GDGCHC 1 cut(s) 635
Bsp1407I TGTACA 1 cut(s) 708
Bsp143I GATC 5 cut(s) 516, 655, 1024, 1029, 1192
Bsp19I CCATGG 1 cut(s) 1346
BspACI CCGC 6 cut(s) 71, 76, 435, 507, 548, 556
BspANI GGCC 5 cut(s) 70, 170, 530, 673, 945
BspCNI CTCAG 3 cut(s) 402, 601, 714
BspHI TCATGA 2 cut(s) 1060, 1189
BspLI GGNNCC 3 cut(s) 253, 360, 946
BspMAI CTGCAG 2 cut(s) 1335, 1395
BspMI ACCTGC 3 cut(s) 85, 1338, 1384
BspOI GCTAGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 524, 650, 1019
BspQI GCTCTTC 1 cut(s) 861
BspT104I TTCGAA 2 cut(s) 368, 905
BspTNI GGTCTC 1 cut(s) 1225
BsrDI GCAATG 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 2 cut(s) 266, 1372
BssECI CCNNGG 4 cut(s) 589, 962, 1109, 1346
BssMI GATC 5 cut(s) 516, 655, 1024, 1029, 1192
BssT1I CCWWGG 2 cut(s) 1109, 1346
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 4 cut(s) 151, 598, 731, 928
Bst6I CTCTTC 3 cut(s) 297, 861, 978
BstAUI TGTACA 1 cut(s) 708
BstBI TTCGAA 2 cut(s) 368, 905
BstC8I GCNNGC 2 cut(s) 193, 1214
BstDEI CTNAG 4 cut(s) 410, 609, 701, 823
BstDSI CCRYGG 1 cut(s) 1346
BstF5I GGATG 2 cut(s) 429, 486
BstHHI GCGC 1 cut(s) 1318
BstKTI GATC 5 cut(s) 519, 658, 1027, 1032, 1195
BstMAI GTCTC 2 cut(s) 231, 1225
BstMBI GATC 5 cut(s) 516, 655, 1024, 1029, 1192
BstMCI CGRYCG 3 cut(s) 71, 80, 153
BstMWI GCNNNNNNNGC 3 cut(s) 188, 393, 504
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 4 cut(s) 25, 409, 1136, 1171
BstSCI CCNGG 5 cut(s) 354, 753, 961, 962, 1042
BstSFI CTRYAG 2 cut(s) 1331, 1391
BstV1I GCAGC 4 cut(s) 60, 194, 444, 1345
BstXI CCANNNNNNTGG 1 cut(s) 1347
BstZI CGGCCG 1 cut(s) 68
BsuRI GGCC 5 cut(s) 70, 170, 530, 673, 945
BtgI CCRYGG 1 cut(s) 1346
BtgZI GCGATG 1 cut(s) 991
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 2 cut(s) 429, 486
BtsI GCAGTG 1 cut(s) 1090
BtsIMutI CAGTG 2 cut(s) 537, 1090
BveI ACCTGC 3 cut(s) 85, 1338, 1384
Cac8I GCNNGC 2 cut(s) 193, 1214
CciI TCATGA 2 cut(s) 1060, 1189
CfoI GCGC 1 cut(s) 1318
Cfr13I GGNCC 2 cut(s) 358, 944
Cfr9I CCCGGG 1 cut(s) 962
CseI GACGC 1 cut(s) 519
Csp6I GTAC 8 cut(s) 207, 213, 316, 391, 454, 709, 796, 1362
CviQI GTAC 8 cut(s) 207, 213, 316, 391, 454, 709, 796, 1362
DdeI CTNAG 4 cut(s) 410, 609, 701, 823
DpnI GATC 5 cut(s) 518, 657, 1026, 1031, 1194
DpnII GATC 5 cut(s) 516, 655, 1024, 1029, 1192
EaeI YGGCCR 3 cut(s) 68, 168, 528
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 3 cut(s) 297, 861, 978
EarI CTCTTC 3 cut(s) 297, 861, 978
Ecl136II GAGCTC 1 cut(s) 633
EclXI CGGCCG 1 cut(s) 68
Eco130I CCWWGG 2 cut(s) 1109, 1346
Eco24I GRGCYC 1 cut(s) 635
Eco31I GGTCTC 1 cut(s) 1225
Eco47I GGWCC 1 cut(s) 358
Eco52I CGGCCG 1 cut(s) 68
Eco53kI GAGCTC 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 944
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 2 cut(s) 1109, 1346
EcoT38I GRGCYC 1 cut(s) 635
ErhI CCWWGG 2 cut(s) 1109, 1346
FaqI GGGAC 1 cut(s) 1213
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 7 cut(s) 71, 74, 183, 433, 436, 557, 1334
FokI GGATG 2 cut(s) 436, 473
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 7 cut(s) 71, 74, 183, 433, 436, 557, 1334
FspBI CTAG 8 cut(s) 186, 192, 204, 210, 272, 560, 743, 884
FspI TGCGCA 1 cut(s) 1317
GlaI GCGC 1 cut(s) 1317
GluI GCNGC 7 cut(s) 71, 74, 183, 433, 436, 557, 1334
HaeIII GGCC 5 cut(s) 70, 170, 530, 673, 945
HapII CCGG 7 cut(s) 164, 298, 313, 644, 754, 963, 1044
HgaI GACGC 1 cut(s) 519
HhaI GCGC 1 cut(s) 1318
Hin6I GCGC 1 cut(s) 1316
HinP1I GCGC 1 cut(s) 1316
HinfI GANTC 6 cut(s) 244, 500, 689, 892, 958, 1387
HpaII CCGG 7 cut(s) 164, 298, 313, 644, 754, 963, 1044
HphI GGTGA 6 cut(s) 439, 562, 574, 916, 1382, 1396
Hpy166II GTNNAC 5 cut(s) 277, 711, 1015, 1021, 1279
Hpy188I TCNGA 8 cut(s) 58, 256, 521, 590, 718, 772, 941, 1029
Hpy188III TCNNGA 4 cut(s) 970, 1061, 1190, 1300
Hpy8I GTNNAC 5 cut(s) 277, 711, 1015, 1021, 1279
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 4 cut(s) 558, 595, 946, 1188
HpyCH4III ACNGT 4 cut(s) 151, 598, 731, 928
HpyCH4IV ACGT 2 cut(s) 678, 1017
HpyCH4V TGCA 7 cut(s) 50, 286, 387, 1130, 1171, 1333, 1393
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 393, 504
HpyF3I CTNAG 4 cut(s) 410, 609, 701, 823
HpySE526I ACGT 2 cut(s) 678, 1017
HspAI GCGC 1 cut(s) 1316
Kzo9I GATC 5 cut(s) 516, 655, 1024, 1029, 1192
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 4 cut(s) 257, 638, 880, 1124
Lsp1109I GCAGC 4 cut(s) 60, 194, 444, 1345
LweI GCATC 3 cut(s) 167, 963, 1029
MaeI CTAG 8 cut(s) 186, 192, 204, 210, 272, 560, 743, 884
MaeII ACGT 2 cut(s) 678, 1017
MaeIII GTNAC 4 cut(s) 338, 445, 598, 922
MalI GATC 5 cut(s) 518, 657, 1026, 1031, 1194
MboI GATC 5 cut(s) 516, 655, 1024, 1029, 1192
MboII GAAGA 5 cut(s) 284, 848, 851, 995, 1153
MhlI GDGCHC 1 cut(s) 635
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 8 cut(s) 119, 364, 901, 1066, 1145, 1245, 1308, 1321
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 2 cut(s) 509, 698
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 9 cut(s) 300, 389, 584, 647, 669, 774, 799, 935, 999
Mox20I TGGCCA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 762
MscI TGGCCA 1 cut(s) 170
MseI TTAA 2 cut(s) 38, 812
MslI CAYNNNNRTG 3 cut(s) 20, 1037, 1137
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 2 cut(s) 73, 435
MspI CCGG 7 cut(s) 164, 298, 313, 644, 754, 963, 1044
MspR9I CCNGG 5 cut(s) 356, 755, 963, 964, 1044
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 3 cut(s) 188, 393, 504
NciI CCSGG 4 cut(s) 755, 963, 964, 1044
NcoI CCATGG 1 cut(s) 1346
NdeII GATC 5 cut(s) 516, 655, 1024, 1029, 1192
NheI GCTAGC 1 cut(s) 191
NlaIV GGNNCC 3 cut(s) 253, 360, 946
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 445, 922
NsbI TGCGCA 1 cut(s) 1317
NspI RCATGY 4 cut(s) 25, 409, 1136, 1171
NspV TTCGAA 2 cut(s) 368, 905
PagI TCATGA 2 cut(s) 1060, 1189
PciI ACATGT 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 4 cut(s) 244, 892, 958, 1387
PkrI GCNGC 7 cut(s) 72, 75, 184, 434, 437, 558, 1335
PleI GAGTC 2 cut(s) 508, 697
PpsI GAGTC 2 cut(s) 508, 697
PscI ACATGT 1 cut(s) 1132
Psp124BI GAGCTC 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 3 cut(s) 253, 360, 946
PspPI GGNCC 2 cut(s) 358, 944
PstI CTGCAG 2 cut(s) 1335, 1395
RsaI GTAC 8 cut(s) 208, 214, 317, 392, 455, 710, 797, 1363
RsaNI GTAC 8 cut(s) 207, 213, 316, 391, 454, 709, 796, 1362
RseI CAYNNNNRTG 3 cut(s) 20, 1037, 1137
SacI GAGCTC 1 cut(s) 635
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 2 cut(s) 38, 812
SatI GCNGC 7 cut(s) 71, 74, 183, 433, 436, 557, 1334
Sau3AI GATC 5 cut(s) 516, 655, 1024, 1029, 1192
Sau96I GGNCC 2 cut(s) 358, 944
ScaI AGTACT 2 cut(s) 208, 214
SchI GAGTC 2 cut(s) 509, 698
ScrFI CCNGG 5 cut(s) 356, 755, 963, 964, 1044
SduI GDGCHC 1 cut(s) 635
SfaNI GCATC 3 cut(s) 167, 963, 1029
SfcI CTRYAG 2 cut(s) 1331, 1391
SfuI TTCGAA 2 cut(s) 368, 905
SinI GGWCC 1 cut(s) 358
SmaI CCCGGG 1 cut(s) 964
SmiMI CAYNNNNRTG 3 cut(s) 20, 1037, 1137
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 8 cut(s) 119, 364, 901, 1066, 1145, 1245, 1308, 1321
SsiI CCGC 6 cut(s) 71, 76, 435, 507, 548, 556
SspMI CTAG 8 cut(s) 186, 192, 204, 210, 272, 560, 743, 884
SstI GAGCTC 1 cut(s) 635
StyD4I CCNGG 5 cut(s) 354, 753, 961, 962, 1042
StyI CCWWGG 2 cut(s) 1109, 1346
TaaI ACNGT 4 cut(s) 151, 598, 731, 928
TaiI ACGT 2 cut(s) 681, 1020
TaqI TCGA 5 cut(s) 351, 368, 779, 905, 1301
TasI AATT 8 cut(s) 119, 364, 901, 1066, 1145, 1245, 1308, 1321
TatI WGTACW 6 cut(s) 206, 212, 390, 453, 708, 795
TauI GCSGC 3 cut(s) 73, 438, 559
TfiI GAWTC 4 cut(s) 244, 892, 958, 1387
Tru1I TTAA 2 cut(s) 38, 812
Tru9I TTAA 2 cut(s) 38, 812
TscAI CASTG 2 cut(s) 544, 1090
TseFI GTSAC 2 cut(s) 445, 922
TseI GCWGC 4 cut(s) 73, 182, 432, 1333
Tsp45I GTSAC 2 cut(s) 445, 922
TspDTI ATGAA 6 cut(s) 26, 456, 681, 735, 926, 1178
TspGWI ACGGA 1 cut(s) 893
TspMI CCCGGG 1 cut(s) 962
TspRI CASTG 2 cut(s) 544, 1090
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 3 cut(s) 1066, 1245, 1321
XceI RCATGY 4 cut(s) 25, 409, 1136, 1171
XmaI CCCGGG 1 cut(s) 962
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 8 cut(s) 186, 192, 204, 210, 272, 560, 743, 884
ZrmI AGTACT 2 cut(s) 208, 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.