Rh4CG239300

Inosine-uridine preferring nucleoside hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0020541
Physical Location & Seq
Reverse (-)
48393464 .. 48401281
7818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG239300.1

Sequence Viewer

Length: 690 bp
ATGGCTAGCTGCCGTGACTGGCTCGAAGATGGCGTTTGTCCGCTGAAAAAGGCTAGCGGTGTCGCTGGAGCTGCTGGCGGATCTAAGGTTACCATATTGACCAATGGACCATTGACTCTAGCAAGCATTATTTTGTCGGAAAATAACACTAGCTCTCTAATTCAGGATGTGTATATAGTTGGAGGACATATCAGCCGCAGTGAAAATGACAGAGGAAATGTGTTTTCGATTCCTTCCAGTGAATATGCAGAGTTCAATATGTTTCTTGACCCGTTGGCTGCAAAGACTGTGTTTGATTCCTCACTTAACATCACACTCATTCCTCTTGGCATCCAGCAGAGAGTTAGTTATTTTCCAAAAATATTAAAAAGGCTGCGCAGCACGAAGAAGACAACACCAGAAGCACTGTTTGCCCGGCGATTGCTGTCAAGGCTATACCGTTTGCAACAATTGCACCATAGCTATCACCACATGGGAACTTTCTTGGGGAAACTTCTTGGTGCAGTTCTCTTGGACGGTGATTCTCATCTGAGCCAAAGCTTCAAACTCAAACACATCAAAGTTTTTGCTGATGATATTGAATCCAGAGATGGGGAAATTTTGATTCATCAAAACCAAGGAAAATTAGTCAAAGTATCGGACAAAGTAAATCCCAGGGCATATTATAAACTTTTCGCAGAAACGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

229

Amino Acids

25.36

Weight (kDa)

9.39

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IU_nuc_hydro PF01156 27 - 221 1.4e-19 Inosine-uridine preferring nucleoside hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020541)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0420381
rosa_multiflora Rmu_sc0002248.1_g000006
rosa_roxburghii Rroxscaffold_5G00363020
rosa_samantha Rh4AG224900 Rh4CG239300 Rh4DG223400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
Acc16I TGCGCA 1 cut(s) 377
AciI CCGC 4 cut(s) 41, 57, 78, 196
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 597
AfiI CCNNNNNNNGG 1 cut(s) 591
AgsI TTSAA 3 cut(s) 256, 544, 581
AjnI CCWGG 1 cut(s) 653
AluBI AGCT 5 cut(s) 9, 71, 153, 462, 540
AluI AGCT 5 cut(s) 9, 71, 153, 462, 540
AlwI GGATC 1 cut(s) 88
ApeKI GCWGC 5 cut(s) 9, 71, 278, 373, 378
ApoI RAATTY 1 cut(s) 597
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 458, 530
AsuNHI GCTAGC 2 cut(s) 5, 53
AvaII GGWCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 4 cut(s) 58, 265, 360, 390
BccI CCATC 2 cut(s) 23, 584
BciT130I CCWGG 1 cut(s) 655
BcnI CCSGG 1 cut(s) 415
BfaI CTAG 4 cut(s) 6, 54, 119, 150
BisI GCNGC 6 cut(s) 10, 72, 196, 279, 374, 379
BlsI GCNGC 6 cut(s) 11, 73, 197, 280, 375, 380
Bme1390I CCNGG 2 cut(s) 415, 655
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmrFI CCNGG 2 cut(s) 415, 655
BmsI GCATC 1 cut(s) 339
BmtI GCTAGC 2 cut(s) 9, 57
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 616, 653, 654
Bsc4I CCNNNNNNNGG 1 cut(s) 591
Bse1I ACTGG 2 cut(s) 23, 237
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 3 cut(s) 616, 653, 654
BseGI GGATG 2 cut(s) 172, 330
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 1 cut(s) 591
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 2 cut(s) 23, 237
BseXI GCAGC 4 cut(s) 58, 265, 360, 390
BsgI GTGCAG 1 cut(s) 522
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 591
Bsp143I GATC 1 cut(s) 80
BspACI CCGC 4 cut(s) 41, 57, 78, 196
BspCNI CTCAG 1 cut(s) 522
BspOI GCTAGC 2 cut(s) 9, 57
BspPI GGATC 1 cut(s) 88
BsrI ACTGG 2 cut(s) 23, 237
BssECI CCNNGG 3 cut(s) 616, 653, 654
BssMI GATC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 4 cut(s) 289, 408, 440, 518
BstAPI GCANNNNNTGC 2 cut(s) 410, 451
BstC8I GCNNGC 4 cut(s) 7, 55, 76, 124
BstDEI CTNAG 3 cut(s) 84, 530, 687
BstEII GGTNACC 1 cut(s) 88
BstF5I GGATG 2 cut(s) 172, 330
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 1 cut(s) 83
BstMBI GATC 1 cut(s) 80
BstMWI GCNNNNNNNGC 5 cut(s) 71, 410, 430, 451, 683
BstNI CCWGG 1 cut(s) 655
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 413, 653
BstV1I GCAGC 4 cut(s) 58, 265, 360, 390
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BtsCI GGATG 2 cut(s) 172, 330
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 3 cut(s) 205, 244, 404
Cac8I GCNNGC 4 cut(s) 7, 55, 76, 124
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 1 cut(s) 107
CviAII CATG 1 cut(s) 472
DdeI CTNAG 3 cut(s) 84, 530, 687
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 107
Eco91I GGTNACC 1 cut(s) 88
EcoO65I GGTNACC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 1 cut(s) 475
FatI CATG 1 cut(s) 471
Fnu4HI GCNGC 6 cut(s) 10, 72, 196, 279, 374, 379
FokI GGATG 2 cut(s) 179, 317
Fsp4HI GCNGC 6 cut(s) 10, 72, 196, 279, 374, 379
FspBI CTAG 4 cut(s) 6, 54, 119, 150
FspI TGCGCA 1 cut(s) 377
GlaI GCGC 1 cut(s) 377
GluI GCNGC 6 cut(s) 10, 72, 196, 279, 374, 379
GsuI CTGGAG 1 cut(s) 87
HapII CCGG 1 cut(s) 415
HhaI GCGC 1 cut(s) 378
Hin1II CATG 1 cut(s) 475
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 6 cut(s) 115, 229, 296, 521, 581, 604
HpaII CCGG 1 cut(s) 415
HphI GGTGA 2 cut(s) 458, 530
Hpy188I TCNGA 3 cut(s) 139, 531, 640
Hpy188III TCNNGA 3 cut(s) 164, 266, 585
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 4 cut(s) 289, 408, 440, 518
HpyCH4V TGCA 5 cut(s) 248, 281, 445, 454, 503
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 410, 430, 451, 683
HpyF3I CTNAG 3 cut(s) 84, 530, 687
Hsp92II CATG 1 cut(s) 475
HspAI GCGC 1 cut(s) 376
Kzo9I GATC 1 cut(s) 80
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 4 cut(s) 58, 265, 360, 390
LweI GCATC 1 cut(s) 339
MaeI CTAG 4 cut(s) 6, 54, 119, 150
MaeIII GTNAC 2 cut(s) 14, 88
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MboII GAAGA 3 cut(s) 38, 397, 400
MfeI CAATTG 1 cut(s) 449
MflI RGATCY 1 cut(s) 80
MluCI AATT 4 cut(s) 159, 449, 597, 623
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 2 cut(s) 117, 160
MnlI CCTC 4 cut(s) 176, 206, 310, 333
MseI TTAA 2 cut(s) 306, 365
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 2 cut(s) 415, 655
MunI CAATTG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 655
MwoI GCNNNNNNNGC 5 cut(s) 71, 410, 430, 451, 683
NciI CCSGG 1 cut(s) 415
NdeII GATC 1 cut(s) 80
NheI GCTAGC 2 cut(s) 5, 53
NlaIII CATG 1 cut(s) 475
NmuCI GTSAC 1 cut(s) 14
NsbI TGCGCA 1 cut(s) 377
PasI CCCWGGG 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 5 cut(s) 229, 296, 521, 581, 604
PkrI GCNGC 6 cut(s) 11, 73, 197, 280, 375, 380
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 653
PspEI GGTNACC 1 cut(s) 88
PspGI CCWGG 1 cut(s) 653
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 1 cut(s) 80
SaqAI TTAA 2 cut(s) 306, 365
SatI GCNGC 6 cut(s) 10, 72, 196, 279, 374, 379
Sau3AI GATC 1 cut(s) 80
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 415, 655
SetI ASST 6 cut(s) 11, 73, 90, 155, 464, 542
SfaNI GCATC 1 cut(s) 339
SinI GGWCC 1 cut(s) 107
Sse9I AATT 4 cut(s) 159, 449, 597, 623
SsiI CCGC 4 cut(s) 41, 57, 78, 196
SspI AATATT 1 cut(s) 363
SspMI CTAG 4 cut(s) 6, 54, 119, 150
StyD4I CCNGG 2 cut(s) 413, 653
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 4 cut(s) 289, 408, 440, 518
TaqI TCGA 2 cut(s) 24, 227
TasI AATT 4 cut(s) 159, 449, 597, 623
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 5 cut(s) 229, 296, 521, 581, 604
Tru1I TTAA 2 cut(s) 306, 365
Tru9I TTAA 2 cut(s) 306, 365
TscAI CASTG 3 cut(s) 205, 244, 411
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 5 cut(s) 9, 71, 278, 373, 378
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 596
TspRI CASTG 3 cut(s) 205, 244, 411
VpaK11BI GGWCC 1 cut(s) 107
XapI RAATTY 1 cut(s) 597
XspI CTAG 4 cut(s) 6, 54, 119, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.