Rh5AG134000

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0021438
Physical Location & Seq
Forward (+)
13646195 .. 13660097
13903 bp
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UTR
Exon/CDS
Intron
Rh5AG134000.1

Sequence Viewer

Length: 645 bp
ATGGAGGAGTGTGAGGATGATTCTAGCTCTAATGAGGCTGAGGAGCCGGAACCAGTTGTTCATGCGTTGGTGCCTTATAACTCAAGTGGTCAAGATTGGAATGATCTGATTGAGGAGGACCAAATTCCTACTCGAGGTCAGATTAATTTTTCTGAAATTAATCAGACTCCGGTTACTCCTCCCAGAGGACAGCTTGGTACATGTCGTCCAACCCAGAAGCTTCTATCACCTTCGCCCGATCTCCAACCCGTTTACCTTCGTGAACGACTTGACGCTGAGGAGATACCGGTTCAGTATGGTGGCTTCAAGAGAGAAAATGACACCGAGTTCTTCTCCTCCGACGATGGTTCTGTTTCTGAGCTAATTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATCACATTGGTTTCGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGAGTTTGTTCCTAGTGATGAAGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAGATCGGAGCTAATGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACGTCGAGCAAGAAGAAGAGGGTGCTGTATCGGCACAAGTCCAACAAGTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

214

Amino Acids

23.89

Weight (kDa)

4.74

Isoelectric Point (pI)

63.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOLD_PATL1_C PF25099 125 - 202 2e-21 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021438)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0018221
rosa_multiflora Rmu_sc0006581.1_g000001 Rmu_sc0018521.1_g000001
rosa_samantha Rh5AG134000 Rh5BG132500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 70
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 134, 185
AflIII ACRYGT 2 cut(s) 200, 588
AgeI ACCGGT 1 cut(s) 286
AgsI TTSAA 1 cut(s) 307
AjiI CACGTC 1 cut(s) 591
AjnI CCWGG 1 cut(s) 559
AloI GAACNNNNNNTCC 2 cut(s) 444, 476
AluBI AGCT 5 cut(s) 27, 193, 220, 361, 518
AluI AGCT 5 cut(s) 27, 193, 220, 361, 518
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 2 cut(s) 372, 527
ApoI RAATTY 1 cut(s) 123
AseI ATTAAT 2 cut(s) 144, 159
AsiGI ACCGGT 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 118, 527
AsuHPI GGTGA 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 118
BanI GGYRCC 1 cut(s) 70
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
BbvCI CCTCAGC 2 cut(s) 39, 276
BccI CCATC 2 cut(s) 338, 427
BciT130I CCWGG 1 cut(s) 561
BclI TGATCA 1 cut(s) 400
BfaI CTAG 2 cut(s) 24, 465
Bme1390I CCNGG 1 cut(s) 561
Bme18I GGWCC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 132
BmgBI CACGTC 1 cut(s) 591
BmgT120I GGNCC 2 cut(s) 118, 527
BmiI GGNNCC 3 cut(s) 45, 51, 72
BmrFI CCNGG 1 cut(s) 561
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu10I CCTNAGC 2 cut(s) 39, 276
BpuEI CTTGAG 2 cut(s) 67, 353
BsaWI WCCGGW 2 cut(s) 169, 286
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 185
Bse118I RCCGGY 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 1 cut(s) 561
BseGI GGATG 3 cut(s) 22, 422, 438
BseLI CCNNNNNNNGG 2 cut(s) 134, 185
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 3 cut(s) 30, 267, 348
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 6 cut(s) 20, 56, 128, 168, 293, 325
BshFI GGCC 2 cut(s) 374, 529
BshNI GGYRCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 4 cut(s) 47, 170, 287, 375
BslI CCNNNNNNNGG 2 cut(s) 134, 185
BsnI GGCC 2 cut(s) 374, 529
BsoBI CYCGRG 1 cut(s) 132
Bsp143I GATC 5 cut(s) 103, 238, 377, 400, 510
BspANI GGCC 2 cut(s) 374, 529
BspCNI CTCAG 3 cut(s) 31, 268, 349
BspLI GGNNCC 3 cut(s) 45, 51, 72
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 1 cut(s) 286
BsrI ACTGG 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 286
BssMI GATC 5 cut(s) 103, 238, 377, 400, 510
Bst2UI CCWGG 1 cut(s) 561
Bst6I CTCTTC 1 cut(s) 599
BstDEI CTNAG 3 cut(s) 39, 276, 357
BstENI CCTNNNNNAGG 2 cut(s) 132, 183
BstF5I GGATG 3 cut(s) 22, 422, 438
BstKTI GATC 5 cut(s) 106, 241, 380, 403, 513
BstMBI GATC 5 cut(s) 103, 238, 377, 400, 510
BstMWI GCNNNNNNNGC 1 cut(s) 619
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 559
BsuRI GGCC 2 cut(s) 374, 529
BtrI CACGTC 1 cut(s) 591
BtsCI GGATG 3 cut(s) 22, 422, 438
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 118, 527
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 198
CspAI ACCGGT 1 cut(s) 286
CviAII CATG 2 cut(s) 62, 201
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 3 cut(s) 39, 276, 357
DpnI GATC 5 cut(s) 105, 240, 379, 402, 512
DpnII GATC 5 cut(s) 103, 238, 377, 400, 510
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 132
EcoNI CCTNNNNNAGG 2 cut(s) 132, 183
EcoO109I RGGNCCY 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 559
FaeI CATG 2 cut(s) 65, 204
FaiI YATR 6 cut(s) 63, 78, 202, 297, 392, 481
FatI CATG 2 cut(s) 61, 200
FbaI TGATCA 1 cut(s) 400
FokI GGATG 3 cut(s) 29, 429, 445
FspBI CTAG 2 cut(s) 24, 465
HaeIII GGCC 2 cut(s) 374, 529
HapII CCGG 4 cut(s) 47, 170, 287, 375
HgaI GACGC 1 cut(s) 281
Hin1II CATG 2 cut(s) 65, 204
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 2 cut(s) 20, 166
HpaII CCGG 4 cut(s) 47, 170, 287, 375
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 2 cut(s) 253, 263
Hpy188III TCNNGA 5 cut(s) 92, 260, 307, 574, 583
Hpy8I GTNNAC 2 cut(s) 253, 263
Hpy99I CGWCG 2 cut(s) 344, 595
HpyAV CCTTC 4 cut(s) 240, 266, 467, 518
HpyCH4IV ACGT 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 619
HpyF3I CTNAG 3 cut(s) 39, 276, 357
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 2 cut(s) 65, 204
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 103, 238, 377, 400, 510
LmnI GCTCC 2 cut(s) 43, 515
MaeI CTAG 2 cut(s) 24, 465
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 1 cut(s) 172
MalI GATC 5 cut(s) 105, 240, 379, 402, 512
MboI GATC 5 cut(s) 103, 238, 377, 400, 510
MboII GAAGA 7 cut(s) 322, 511, 514, 517, 520, 613, 616
MluCI AATT 5 cut(s) 123, 145, 156, 363, 443
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 3 cut(s) 233, 268, 363
MseI TTAA 2 cut(s) 144, 159
MspI CCGG 4 cut(s) 47, 170, 287, 375
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 1 cut(s) 619
NdeII GATC 5 cut(s) 103, 238, 377, 400, 510
NlaIII CATG 2 cut(s) 65, 204
NlaIV GGNNCC 3 cut(s) 45, 51, 72
NspI RCATGY 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 132
PciI ACATGT 1 cut(s) 200
PfeI GAWTC 1 cut(s) 20
PinAI ACCGGT 1 cut(s) 286
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
PscI ACATGT 1 cut(s) 200
PshBI ATTAAT 2 cut(s) 144, 159
PsiI TTATAA 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 45, 51, 72
PspPI GGNCC 2 cut(s) 118, 527
PspXI VCTCGAGB 1 cut(s) 132
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
SaqAI TTAA 2 cut(s) 144, 159
Sau3AI GATC 5 cut(s) 103, 238, 377, 400, 510
Sau96I GGNCC 2 cut(s) 118, 527
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 132
SinI GGWCC 1 cut(s) 118
SlaI CTCGAG 1 cut(s) 132
SmlI CTYRAG 3 cut(s) 82, 132, 368
SmoI CTYRAG 3 cut(s) 82, 132, 368
Sse9I AATT 5 cut(s) 123, 145, 156, 363, 443
SspMI CTAG 2 cut(s) 24, 465
StyD4I CCNGG 1 cut(s) 559
TaiI ACGT 1 cut(s) 593
TaqI TCGA 3 cut(s) 133, 582, 593
TasI AATT 5 cut(s) 123, 145, 156, 363, 443
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 144, 159
Tru9I TTAA 2 cut(s) 144, 159
TspDTI ATGAA 3 cut(s) 50, 486, 537
VpaK11BI GGWCC 1 cut(s) 118
VspI ATTAAT 2 cut(s) 144, 159
XagI CCTNNNNNAGG 2 cut(s) 132, 183
XapI RAATTY 1 cut(s) 123
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 132
XspI CTAG 2 cut(s) 24, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.