Rh5BG132500

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0021438
Physical Location & Seq
Forward (+)
13058004 .. 13058723
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG132500.1

Sequence Viewer

Length: 399 bp
ATGCACAAGTACATTCCAGCTGAGGAGATACCGGTTCAGTATGGTGGCTTCAAGAGAGAAAATGACACCGAGTTCTTCTCCTCCGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATGTAGCTGAGGACATTGGTTTGGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGAGTTTGTTCCTAGTGATGAAGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAGATCGGAGCTAATGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACTTCGAGCAAGAAGAAGAGGGTGCTGTACCGGCACAAGTCCAACAAGTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

132

Amino Acids

14.67

Weight (kDa)

5.76

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOLD_PATL1_C PF25099 39 - 120 5.5e-25 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021438)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0018221
rosa_multiflora Rmu_sc0006581.1_g000001 Rmu_sc0018521.1_g000001
rosa_samantha Rh5AG134000 Rh5BG132500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 130
AfaI GTAC 2 cut(s) 11, 371
AgeI ACCGGT 1 cut(s) 31
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 1 cut(s) 313
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 4 cut(s) 20, 106, 152, 272
AluI AGCT 4 cut(s) 20, 106, 152, 272
AlwI GGATC 1 cut(s) 130
AoxI GGCC 2 cut(s) 117, 281
AsiGI ACCGGT 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 281
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BbvCI CCTCAGC 2 cut(s) 21, 153
BccI CCATC 2 cut(s) 83, 181
BciT130I CCWGG 1 cut(s) 315
BfaI CTAG 1 cut(s) 219
Bme1390I CCNGG 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 62, 94
Bpu10I CCTNAGC 2 cut(s) 21, 153
BpuEI CTTGAG 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bse118I RCCGGY 2 cut(s) 31, 372
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 176, 192
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 3 cut(s) 12, 93, 144
BseRI GAGGAG 2 cut(s) 38, 70
BshFI GGCC 2 cut(s) 119, 283
BshTI ACCGGT 1 cut(s) 31
BsiSI CCGG 3 cut(s) 32, 120, 373
BsnI GGCC 2 cut(s) 119, 283
Bsp143I GATC 2 cut(s) 122, 264
BspANI GGCC 2 cut(s) 119, 283
BspCNI CTCAG 3 cut(s) 13, 94, 145
BspPI GGATC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 2 cut(s) 31, 372
BssAI RCCGGY 2 cut(s) 31, 372
BssMI GATC 2 cut(s) 122, 264
Bst2UI CCWGG 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 353
BstDEI CTNAG 3 cut(s) 21, 102, 153
BstF5I GGATG 2 cut(s) 176, 192
BstKTI GATC 2 cut(s) 125, 267
BstMBI GATC 2 cut(s) 122, 264
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BsuRI GGCC 2 cut(s) 119, 283
BtsCI GGATG 2 cut(s) 176, 192
Cfr10I RCCGGY 2 cut(s) 31, 372
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 10, 370
CspAI ACCGGT 1 cut(s) 31
CviJI RGCY 8 cut(s) 20, 48, 106, 119, 152, 272, 283, 313
CviKI_1 RGCY 8 cut(s) 20, 48, 106, 119, 152, 272, 283, 313
CviQI GTAC 2 cut(s) 10, 370
DdeI CTNAG 3 cut(s) 21, 102, 153
DpnI GATC 2 cut(s) 124, 266
DpnII GATC 2 cut(s) 122, 264
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 313
FaiI YATR 3 cut(s) 42, 137, 235
FokI GGATG 2 cut(s) 183, 199
FspBI CTAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 119, 283
HapII CCGG 3 cut(s) 32, 120, 373
HpaII CCGG 3 cut(s) 32, 120, 373
Hpy188I TCNGA 5 cut(s) 85, 103, 252, 269, 302
Hpy188III TCNNGA 3 cut(s) 52, 328, 337
HpyAV CCTTC 2 cut(s) 221, 272
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 3 cut(s) 21, 102, 153
Kzo9I GATC 2 cut(s) 122, 264
LmnI GCTCC 1 cut(s) 269
LpnPI CCDG 7 cut(s) 30, 45, 133, 300, 327, 341, 386
MaeI CTAG 1 cut(s) 219
MalI GATC 2 cut(s) 124, 266
MboI GATC 2 cut(s) 122, 264
MboII GAAGA 8 cut(s) 67, 98, 265, 268, 271, 274, 367, 370
MluCI AATT 1 cut(s) 197
MnlI CCTC 4 cut(s) 16, 91, 148, 354
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 3 cut(s) 32, 120, 373
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 373
NdeII GATC 2 cut(s) 122, 264
PinAI ACCGGT 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspPI GGNCC 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 11, 371
RsaNI GTAC 2 cut(s) 10, 370
Sau3AI GATC 2 cut(s) 122, 264
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 315
SetI ASST 5 cut(s) 22, 108, 154, 274, 324
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 1 cut(s) 197
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 313
TaqI TCGA 2 cut(s) 336, 347
TasI AATT 1 cut(s) 197
TatI WGTACW 1 cut(s) 9
TspDTI ATGAA 2 cut(s) 240, 291
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.