Rh5AG375300

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020748
Physical Location & Seq
Forward (+)
63810249 .. 63813117
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG375300.1

Sequence Viewer

Length: 1077 bp
ATGGGTTCTGCTGTGGGAGGAGGCTGGAATACGCAGGCTGTAACTTACAGCCACTGGTGGGGGTTGACGTGGCGCGAAGAGAGCCACAAAATGGATCAATGTCAGGGTTCAGCAAATCTGTTCACACTGTCACAAAAACTGAGGACCCTGGTGGCTGCGTTTAGGAATGACATAGCTCTCTTGTATCGGGATGAAATTAGTTGTCTTCAGCCACGAATATATGTTAATTCACCGGTCATTAACTATGTGGGTGAATATATGAATTGCAAAGGTTCTAATAGTTGGTACTTCTCGACATTCTTTGAGAAAAAGGCAAGTGCATATGACTCTACAGAGACATTTCAAGACTGGATTGAGAATACAAGAAGTGTATGCAATCTTGATAGATTTAATGGACAACTCCACAATTGTGAGAAGATATTTGTGCTGTTGCATGAGGAAGGAGATGGAGGGCATTGGTATCTCATGGTGGTTCTTGTTAGGGAAAAAGTTGCTGAGATATGGGATAGCTGTCCCTCTGTGGAGAAAGAGGCCAGCAGATGGGGACATGCAATAACGACTCTCCGACACCTAGACCATGTATTGTCTCTTGAGACCGTCAATTACAAAGGTGCTAAAACTTCATTTGGTACATTCCATATCATCGTTCCTGAAGGGGTTCCTCTCCAAAAGAATGGATATGATTGCGGAATATATGTGATTAAAAATATGCTCCAATATGGGAAAAAATGGGTCGAAGAGTATGAATCACATGAGGAGAGGGCAATGATTCTGTTAGATTGCATGAAATTCTCTCACAATGACATGCACAGCCTTATACCTGAAGGCAACATGGGAAGTGGTTATGTTGTTGAACATGACAAAGAGGAGGTTTGTGAAAAGGGAAAACTCAATATAACCAAGCCATTTTCAGCATGGGATGCATCTGATTTCTGTGACTTACCAACCTTGGTTGATATATTTGGAATGTACGGTGATGGGGGACCTAGTGGAGGAAGTAAGATGGGCTTAGTTGCTGATGATGTGGGAGGTGAGTCAGGGGAAATCTCAGTTTTAAAATCTCTTGAGTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

358

Amino Acids

40.4

Weight (kDa)

5.14

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 89 - 238 7.1e-15 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020748)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005394.1_g000007
rosa_samantha Rh2AG647100 Rh2BG657800 Rh5AG375300 Rh5CG367100
rosa_wichuraiana Rw1G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 91, 540
AccII CGCG 1 cut(s) 75
AciI CCGC 1 cut(s) 687
AclWI GGATC 1 cut(s) 102
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 3 cut(s) 191, 672, 843
AfaI GTAC 3 cut(s) 287, 631, 971
AfiI CCNNNNNNNGG 4 cut(s) 58, 91, 540, 992
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 2 cut(s) 344, 854
AjiI CACGTC 1 cut(s) 69
AjnI CCWGG 1 cut(s) 147
AleI CACNNNNGTG 1 cut(s) 408
AluBI AGCT 2 cut(s) 176, 510
AluI AGCT 2 cut(s) 176, 510
Alw26I GTCTC 3 cut(s) 329, 587, 591
AlwI GGATC 1 cut(s) 102
AlwNI CAGNNNCTG 1 cut(s) 54
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 788
AsiGI ACCGGT 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 657
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 144, 983
AsuHPI GGTGA 4 cut(s) 222, 263, 986, 1043
AvaII GGWCC 2 cut(s) 144, 983
BbsI GAAGAC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 142
BccI CCATC 4 cut(s) 440, 534, 971, 997
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 3 cut(s) 329, 587, 591
BfaI CTAG 2 cut(s) 572, 987
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 2 cut(s) 144, 983
BmgBI CACGTC 1 cut(s) 69
BmgT120I GGNCC 2 cut(s) 144, 983
BmiI GGNNCC 3 cut(s) 146, 660, 984
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 2 cut(s) 910, 932
BpiI GAAGAC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 611
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 147, 948
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 91, 540, 992
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 59, 353
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 147, 948
BseGI GGATG 2 cut(s) 196, 925
BseLI CCNNNNNNNGG 4 cut(s) 58, 91, 540, 992
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 3 cut(s) 131, 486, 1062
BseNI ACTGG 2 cut(s) 59, 353
BseRI GAGGAG 3 cut(s) 33, 770, 881
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 232
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 3 cut(s) 498, 558, 996
BslI CCNNNNNNNGG 4 cut(s) 58, 91, 540, 992
BsmAI GTCTC 3 cut(s) 329, 587, 591
BsmFI GGGAC 3 cut(s) 498, 558, 996
BsnI GGCC 1 cut(s) 533
Bso31I GGTCTC 1 cut(s) 587
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 1 cut(s) 687
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 3 cut(s) 132, 487, 1061
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 146, 660, 984
BspPI GGATC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 2 cut(s) 59, 353
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 2 cut(s) 147, 948
BssMI GATC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 129, 598, 974
Bst6I CTCTTC 2 cut(s) 72, 732
BstAPI GCANNNNNTGC 1 cut(s) 920
BstC8I GCNNGC 2 cut(s) 36, 535
BstDEI CTNAG 4 cut(s) 140, 495, 1009, 1048
BstENI CCTNNNNNAGG 1 cut(s) 990
BstF5I GGATG 2 cut(s) 196, 925
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 3 cut(s) 329, 587, 591
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 2 cut(s) 81, 920
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 2 cut(s) 551, 808
BstSCI CCNGG 1 cut(s) 147
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 674
BsuRI GGCC 1 cut(s) 533
BtrI CACGTC 1 cut(s) 69
BtsCI GGATG 2 cut(s) 196, 925
BtsIMutI CAGTG 2 cut(s) 52, 125
Cac8I GCNNGC 2 cut(s) 36, 535
CaiI CAGNNNCTG 1 cut(s) 54
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 144, 983
Csp6I GTAC 3 cut(s) 286, 630, 970
CspAI ACCGGT 1 cut(s) 232
CviQI GTAC 3 cut(s) 286, 630, 970
DdeI CTNAG 4 cut(s) 140, 495, 1009, 1048
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
DraI TTTAAA 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 72, 732
EarI CTCTTC 2 cut(s) 72, 732
Eco130I CCWWGG 1 cut(s) 948
Eco31I GGTCTC 1 cut(s) 587
Eco47I GGWCC 2 cut(s) 144, 983
Eco57I CTGAAG 3 cut(s) 191, 672, 843
EcoNI CCTNNNNNAGG 1 cut(s) 990
EcoO109I RGGNCCY 2 cut(s) 144, 983
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 948
EcoT22I ATGCAT 1 cut(s) 925
ErhI CCWWGG 1 cut(s) 948
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FaqI GGGAC 3 cut(s) 498, 558, 996
FauNDI CATATG 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 2 cut(s) 203, 932
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 2 cut(s) 572, 987
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 156
HaeIII GGCC 1 cut(s) 533
HapII CCGG 1 cut(s) 233
HhaI GCGC 1 cut(s) 75
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 6 cut(s) 326, 559, 746, 769, 1034, 1067
HpaII CCGG 1 cut(s) 233
HphI GGTGA 4 cut(s) 222, 263, 986, 1043
Hpy166II GTNNAC 2 cut(s) 66, 123
Hpy188I TCNGA 3 cut(s) 566, 928, 1072
Hpy188III TCNNGA 7 cut(s) 188, 292, 344, 380, 590, 650, 1064
Hpy8I GTNNAC 2 cut(s) 66, 123
HpyAV CCTTC 3 cut(s) 434, 647, 818
HpyCH4III ACNGT 3 cut(s) 129, 598, 974
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 8 cut(s) 267, 320, 375, 433, 551, 783, 808, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 920
HpyF3I CTNAG 4 cut(s) 140, 495, 1009, 1048
HpySE526I ACGT 1 cut(s) 68
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 717
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 2 cut(s) 910, 932
MaeI CTAG 2 cut(s) 572, 987
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 40, 129, 935
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 4 cut(s) 89, 197, 427, 749
MfeI CAATTG 1 cut(s) 406
MluCI AATT 6 cut(s) 195, 226, 262, 406, 601, 788
MlyI GAGTC 4 cut(s) 320, 553, 1043, 1076
MmeI TCCRAC 1 cut(s) 589
Mph1103I ATGCAT 1 cut(s) 925
MroXI GAANNNNTTC 1 cut(s) 657
MseI TTAA 5 cut(s) 225, 240, 390, 702, 1055
MslI CAYNNNNRTG 1 cut(s) 408
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 149
MunI CAATTG 1 cut(s) 406
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 81, 920
NdeI CATATG 1 cut(s) 322
NdeII GATC 1 cut(s) 94
NlaIV GGNNCC 3 cut(s) 146, 660, 984
NmuCI GTSAC 2 cut(s) 129, 935
NsiI ATGCAT 1 cut(s) 925
NspI RCATGY 2 cut(s) 551, 808
OliI CACNNNNGTG 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 746, 769
PflMI CCANNNNNTGG 2 cut(s) 91, 540
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 4 cut(s) 320, 553, 1042, 1075
PpsI GAGTC 4 cut(s) 320, 553, 1042, 1075
PpuMI RGGWCCY 2 cut(s) 144, 983
Psp5II RGGWCCY 2 cut(s) 144, 983
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 3 cut(s) 146, 660, 984
PspPI GGNCC 2 cut(s) 144, 983
PspPPI RGGWCCY 2 cut(s) 144, 983
PstNI CAGNNNCTG 1 cut(s) 54
RsaI GTAC 3 cut(s) 287, 631, 971
RsaNI GTAC 3 cut(s) 286, 630, 970
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 5 cut(s) 225, 240, 390, 702, 1055
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 2 cut(s) 144, 983
SchI GAGTC 4 cut(s) 320, 553, 1043, 1076
ScrFI CCNGG 1 cut(s) 149
SfaNI GCATC 2 cut(s) 910, 932
SfcI CTRYAG 1 cut(s) 330
SinI GGWCC 2 cut(s) 144, 983
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 590, 1064
SmoI CTYRAG 2 cut(s) 590, 1064
Sse9I AATT 6 cut(s) 195, 226, 262, 406, 601, 788
SsiI CCGC 1 cut(s) 687
SspMI CTAG 2 cut(s) 572, 987
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 948
TaaI ACNGT 3 cut(s) 129, 598, 974
TaiI ACGT 1 cut(s) 71
TaqI TCGA 2 cut(s) 293, 735
TasI AATT 6 cut(s) 195, 226, 262, 406, 601, 788
TfiI GAWTC 2 cut(s) 746, 769
Tru1I TTAA 5 cut(s) 225, 240, 390, 702, 1055
Tru9I TTAA 5 cut(s) 225, 240, 390, 702, 1055
TscAI CASTG 2 cut(s) 59, 132
TseFI GTSAC 2 cut(s) 129, 935
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 2 cut(s) 129, 935
TspDTI ATGAA 5 cut(s) 207, 275, 612, 759, 800
TspRI CASTG 2 cut(s) 59, 132
Van91I CCANNNNNTGG 2 cut(s) 91, 540
VpaK11BI GGWCC 2 cut(s) 144, 983
XagI CCTNNNNNAGG 1 cut(s) 990
XapI RAATTY 1 cut(s) 788
XceI RCATGY 2 cut(s) 551, 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 912
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 2 cut(s) 572, 987
Zsp2I ATGCAT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.