Rh5AG431400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020001
Physical Location & Seq
Reverse (-)
74143738 .. 74144447
710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG431400.1

Sequence Viewer

Length: 498 bp
ATGACCCAACTCCCCAACAACCCCAACTCCCAAACTACACCTCTCCCCCAGACCTCGATCGTTGCCACCGCGCTTGACAACTTCTACTCTGAGTTCCACTGCATTTCGGAGTTCTTCCACACCGCGTTAGTGAAGAGGATTCAGAATTGCGACGGTGGAAGAGGCGACGAGAATGGCGTCGTTTCTGACCCGGAGTCCGGCGCGATTGTTCCCGTGGAGAAGAACGGCGAGCAGCAGCAGCTGGAGGAGGTCGTGCAGCGGAAGAAATACCCACAGCGATTGAAAGAGCTCGTCCAGTTGGAGGCTTTACAGAGCGAGAATAGGTTGCTGAGAAAAGCTGTGGAGGATCTCCGGCAAGAAATCACCGGAGGGAGTTTGAATTCGGGGATGTACCGTGGAATGGAGAGTAGTGGGAATTGGGGCTCTAGCAACAATGAGAAGAAGTCGCAGGAGGGAGATGTGAGTGAGGAGTTGAAGAAGGCACTGATGGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.28

Weight (kDa)

5.05

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020001)

Species Orthologous Gene IDs
rosa_laevigata RLG00000008319
rosa_multiflora Rmu_sc0001336.1_g000011
rosa_samantha Rh2AG524300 Rh2CG509000 Rh2DG370200 Rh5AG431400 Rh6AG298200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 71, 125, 203
AciI CCGC 3 cut(s) 69, 123, 259
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 1 cut(s) 379
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 3 cut(s) 197, 301, 400
AgsI TTSAA 3 cut(s) 283, 379, 475
AhdI GACNNNNNGTC 1 cut(s) 193
AluBI AGCT 3 cut(s) 241, 289, 338
AluI AGCT 3 cut(s) 241, 289, 338
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 1 cut(s) 354
AlwNI CAGNNNCTG 1 cut(s) 241
ApeKI GCWGC 4 cut(s) 232, 235, 238, 256
ApoI RAATTY 1 cut(s) 379
AspLEI GCGC 2 cut(s) 73, 203
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 355
BanII GRGCYC 2 cut(s) 291, 425
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 4 cut(s) 244, 247, 250, 268
BccI CCATC 1 cut(s) 481
BceAI ACGGC 1 cut(s) 241
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 1 cut(s) 426
BisI GCNGC 4 cut(s) 233, 236, 239, 257
BlsI GCNGC 4 cut(s) 234, 237, 240, 258
Bme1390I CCNGG 1 cut(s) 191
BmeRI GACNNNNNGTC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 213, 394
BsaWI WCCGGW 1 cut(s) 365
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 301, 400
Bse1I ACTGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 213, 394
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 3 cut(s) 197, 301, 400
BseMII CTCAG 2 cut(s) 81, 320
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 2 cut(s) 260, 482
BseXI GCAGC 4 cut(s) 244, 247, 250, 268
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 3 cut(s) 71, 125, 203
Bsh1285I CGRYCG 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 4 cut(s) 191, 198, 352, 366
BslI CCNNNNNNNGG 3 cut(s) 197, 301, 400
Bsp1286I GDGCHC 2 cut(s) 291, 425
Bsp143I GATC 2 cut(s) 57, 346
BspACI CCGC 3 cut(s) 69, 123, 259
BspCNI CTCAG 2 cut(s) 82, 321
BspFNI CGCG 3 cut(s) 71, 125, 203
BspPI GGATC 1 cut(s) 354
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 213, 394
BssMI GATC 2 cut(s) 57, 346
BssNI GRCGYC 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 155, 395
Bst6I CTCTTC 2 cut(s) 128, 154
BstACI GRCGYC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 90, 329
BstDSI CCRYGG 2 cut(s) 213, 394
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 3 cut(s) 71, 125, 203
BstHHI GCGC 2 cut(s) 73, 203
BstKTI GATC 2 cut(s) 60, 349
BstMBI GATC 2 cut(s) 57, 346
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 189
BstUI CGCG 3 cut(s) 71, 125, 203
BstV1I GCAGC 4 cut(s) 244, 247, 250, 268
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BtgI CCRYGG 2 cut(s) 213, 394
BtsCI GGATG 1 cut(s) 393
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 2 cut(s) 97, 482
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 2 cut(s) 73, 203
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 391
CspCI CAANNNNNGTGG 4 cut(s) 55, 90, 261, 296
CviJI RGCY 5 cut(s) 241, 289, 305, 338, 423
CviKI_1 RGCY 5 cut(s) 241, 289, 305, 338, 423
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 2 cut(s) 90, 329
DpnI GATC 2 cut(s) 59, 348
DpnII GATC 2 cut(s) 57, 346
DriI GACNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 128, 154
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 2 cut(s) 128, 154
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 291, 425
Eco53kI GAGCTC 1 cut(s) 289
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 291, 425
Fnu4HI GCNGC 4 cut(s) 233, 236, 239, 257
FokI GGATG 1 cut(s) 400
FriOI GRGCYC 2 cut(s) 291, 425
Fsp4HI GCNGC 4 cut(s) 233, 236, 239, 257
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 72, 202
GluI GCNGC 4 cut(s) 233, 236, 239, 257
GsuI CTGGAG 1 cut(s) 263
HapII CCGG 4 cut(s) 191, 198, 352, 366
HgaI GACGC 1 cut(s) 166
HhaI GCGC 2 cut(s) 73, 203
Hin1I GRCGYC 1 cut(s) 177
Hin6I GCGC 2 cut(s) 71, 201
HinP1I GCGC 2 cut(s) 71, 201
HinfI GANTC 2 cut(s) 139, 194
HpaII CCGG 4 cut(s) 191, 198, 352, 366
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 4 cut(s) 91, 109, 144, 187
Hpy99I CGWCG 3 cut(s) 155, 170, 182
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 2 cut(s) 155, 395
HpyCH4V TGCA 2 cut(s) 102, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 2 cut(s) 90, 329
Hsp92I GRCGYC 1 cut(s) 177
HspAI GCGC 2 cut(s) 71, 201
Kzo9I GATC 2 cut(s) 57, 346
LpnPI CCDG 8 cut(s) 62, 204, 211, 227, 308, 365, 379, 434
Lsp1109I GCAGC 4 cut(s) 244, 247, 250, 268
MaeI CTAG 1 cut(s) 426
MalI GATC 2 cut(s) 59, 348
MboI GATC 2 cut(s) 57, 346
MboII GAAGA 7 cut(s) 106, 145, 171, 232, 274, 451, 487
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 291, 425
MluCI AATT 3 cut(s) 145, 379, 415
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 241, 259
MspI CCGG 4 cut(s) 191, 198, 352, 366
MspR9I CCNGG 1 cut(s) 191
MvnI CGCG 3 cut(s) 71, 125, 203
MwoI GCNNNNNNNGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 191
NdeII GATC 2 cut(s) 57, 346
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 4 cut(s) 234, 237, 240, 258
Ple19I CGATCG 1 cut(s) 60
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 346
PvuI CGATCG 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SacI GAGCTC 1 cut(s) 291
SatI GCNGC 4 cut(s) 233, 236, 239, 257
Sau3AI GATC 2 cut(s) 57, 346
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 2 cut(s) 291, 425
SetI ASST 7 cut(s) 43, 56, 243, 252, 291, 326, 340
Sse9I AATT 3 cut(s) 145, 379, 415
SsiI CCGC 3 cut(s) 69, 123, 259
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 155, 395
TaqI TCGA 1 cut(s) 56
TasI AATT 3 cut(s) 145, 379, 415
TfiI GAWTC 1 cut(s) 139
TscAI CASTG 2 cut(s) 104, 489
TseI GCWGC 4 cut(s) 232, 235, 238, 256
TspRI CASTG 2 cut(s) 104, 489
XapI RAATTY 1 cut(s) 379
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.