Rh5AG453400

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019931
Physical Location & Seq
Reverse (-)
77673840 .. 77675181
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG453400.1

Sequence Viewer

Length: 399 bp
ATGGAGGACAGAAAACAAAAAGAGGTTTGTACTGTCAGGTTCAGGAGGAAAGATGAGTTGGAGAGAGGAAAGCTCTCTGAAATTGACATGGAAAGAGAAGAAGAGTGTGGAATTTGCATGGAGTTGAACAGCAAAGTTGTGTTGCCTAACTGCAGTCATTCATTGTGTTTGAAGTGTTACCGAGATTGGCGTGGGCGGTCTCAGTCATGCCCCTTTTGTCGTGATAGTCTTAAAAGAGTAAATTCAGGCGACCTTTGGATTTACACAGAAAAATCTGATGCTATCGATCTATCATCAATCTTAAGAGAGGACCGCAAGAGATTATTTATGTACATTGATAAGTTGCCTCTAGTTGTTCCAGATCCTGTATATCTTCCCTATGATTCTCATGTCCGGTAA

Protein Analysis

132

Amino Acids

15.71

Weight (kDa)

7.52

Isoelectric Point (pI)

65.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 33 - 79 8.6e-11 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 35 - 74 1.3e-08 Ring finger domain
zf-RING_5 PF14634 35 - 74 1.9e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 35 - 73 6.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 36 - 73 5.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019931)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0077461
rosa_laevigata RLG00000036700
rosa_multiflora Rmu_co8463923.1_g000001
rosa_rugosa Rorug05G0455300
rosa_samantha Rh5AG453400 Rh5BG531800 Rh5DG543700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 196, 313
AclWI GGATC 1 cut(s) 356
AcsI RAATTY 2 cut(s) 111, 241
AfaI GTAC 2 cut(s) 31, 332
AflII CTTAAG 1 cut(s) 301
AgsI TTSAA 2 cut(s) 127, 172
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 1 cut(s) 356
AlwNI CAGNNNCTG 1 cut(s) 365
ApoI RAATTY 2 cut(s) 111, 241
AspS9I GGNCC 1 cut(s) 310
AvaII GGWCC 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 151
BfrI CTTAAG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmsI GCATC 1 cut(s) 268
BplI GAGNNNNNCTC 2 cut(s) 57, 89
Bsa29I ATCGAT 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 285
BseMII CTCAG 1 cut(s) 215
BshVI ATCGAT 1 cut(s) 285
BsiSI CCGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 2 cut(s) 286, 361
BspACI CCGC 2 cut(s) 196, 313
BspCNI CTCAG 1 cut(s) 214
BspDI ATCGAT 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 155
BspPI GGATC 1 cut(s) 356
BspTI CTTAAG 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 330
BssMI GATC 2 cut(s) 286, 361
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 301
BstAUI TGTACA 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 201
BstKTI GATC 2 cut(s) 289, 364
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 286, 361
BstSFI CTRYAG 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 285
CaiI CAGNNNCTG 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 285
Csp6I GTAC 2 cut(s) 30, 331
CviAII CATG 4 cut(s) 88, 118, 207, 389
CviJI RGCY 1 cut(s) 73
CviKI_1 RGCY 1 cut(s) 73
CviQI GTAC 2 cut(s) 30, 331
DdeI CTNAG 1 cut(s) 201
DpnI GATC 2 cut(s) 288, 363
DpnII GATC 2 cut(s) 286, 361
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 310
FaeI CATG 4 cut(s) 91, 121, 210, 392
FaiI YATR 7 cut(s) 89, 119, 208, 329, 370, 381, 390
FatI CATG 4 cut(s) 87, 117, 206, 388
FspBI CTAG 1 cut(s) 350
HapII CCGG 1 cut(s) 394
Hin1II CATG 4 cut(s) 91, 121, 210, 392
HinfI GANTC 1 cut(s) 383
HpaII CCGG 1 cut(s) 394
Hpy188I TCNGA 2 cut(s) 79, 277
Hpy188III TCNNGA 3 cut(s) 43, 221, 359
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 2 cut(s) 117, 153
HpyF3I CTNAG 1 cut(s) 201
Hsp92II CATG 4 cut(s) 91, 121, 210, 392
Kzo9I GATC 2 cut(s) 286, 361
LpnPI CCDG 5 cut(s) 22, 28, 231, 372, 378
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 176
MalI GATC 2 cut(s) 288, 363
MboI GATC 2 cut(s) 286, 361
MboII GAAGA 3 cut(s) 110, 113, 365
MflI RGATCY 1 cut(s) 361
MluCI AATT 3 cut(s) 81, 111, 241
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 5 cut(s) 16, 39, 59, 301, 357
MseI TTAA 2 cut(s) 231, 302
MspCI CTTAAG 1 cut(s) 301
MspI CCGG 1 cut(s) 394
NdeII GATC 2 cut(s) 286, 361
NlaIII CATG 4 cut(s) 91, 121, 210, 392
PfeI GAWTC 1 cut(s) 383
PspPI GGNCC 1 cut(s) 310
PstI CTGCAG 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 2 cut(s) 31, 332
RsaNI GTAC 2 cut(s) 30, 331
SaqAI TTAA 2 cut(s) 231, 302
Sau3AI GATC 2 cut(s) 286, 361
Sau96I GGNCC 1 cut(s) 310
SetI ASST 4 cut(s) 27, 41, 75, 255
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 3 cut(s) 81, 111, 241
SsiI CCGC 2 cut(s) 196, 313
SspMI CTAG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 1 cut(s) 285
TasI AATT 3 cut(s) 81, 111, 241
TatI WGTACW 2 cut(s) 29, 330
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 2 cut(s) 231, 302
Tru9I TTAA 2 cut(s) 231, 302
TspDTI ATGAA 1 cut(s) 150
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 2 cut(s) 111, 241
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.