Rh5BG159400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0022393
Physical Location & Seq
Reverse (-)
16283109 .. 16314274
31166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG159400.1

Sequence Viewer

Length: 447 bp
ATGTGGGATTCAGGCGTTCATGAAACTTTTTTACTTGTTTATTTTGCTGTTAGAGAAGAATTGCATCTGAAAATTGCCAAGCTGCTGCCAACGCCGAGAAGATCTCGGTCCGTACAGGTTGGATTTCACGCCAAGTGGTCGATGGTTGGTGGACACAAGGGTCATCTAGCTATTGTAGACATGAAGCAAATGAGCCTGATTAAAGAATCTCAGGTCCTGCATGTGATGTGGTATTCTTTCATGATGAGACAATGTTTGCTGCAGCCCAGAAAAAGCTTCAGGGCTGATGAAGAGTGGGCCTCGATCAATTCCAGATTTGGCAGCTACTGCAGGGGGGAGCTCGCTGGTCATAAGCGACAAACAGAGCTTACTCTGCTGAAACCCGGGAAGCGACTTCCTTCTACTCTGACACCGGAGTTATTAGTTCATGAGGATATTGCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.13

Weight (kDa)

9.69

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022393)

Species Orthologous Gene IDs
malus_domestica MD08G1003300.v1.1
rosa_chinensis RchiOBHm_Chr1g0336791 RchiOBHm_Chr4g0414881
rosa_samantha Rh5BG159400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 262
AfaI GTAC 1 cut(s) 114
AluBI AGCT 6 cut(s) 82, 170, 276, 324, 340, 367
AluI AGCT 6 cut(s) 82, 170, 276, 324, 340, 367
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 241
AlwNI CAGNNNCTG 2 cut(s) 217, 327
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 5 cut(s) 82, 85, 259, 262, 321
AspS9I GGNCC 3 cut(s) 108, 214, 297
AsuC2I CCSGG 2 cut(s) 384, 385
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 2 cut(s) 108, 214
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 5 cut(s) 69, 72, 246, 274, 333
BccI CCATC 1 cut(s) 136
BcnI CCSGG 2 cut(s) 384, 385
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 3 cut(s) 260, 328, 443
BglII AGATCT 1 cut(s) 101
BisI GCNGC 5 cut(s) 83, 86, 260, 263, 322
BlsI GCNGC 5 cut(s) 84, 87, 261, 264, 323
Bme1390I CCNGG 2 cut(s) 384, 385
Bme18I GGWCC 2 cut(s) 108, 214
BmeT110I CYCGRG 1 cut(s) 383
BmgT120I GGNCC 3 cut(s) 108, 214, 297
BmrFI CCNGG 2 cut(s) 384, 385
BmsI GCATC 1 cut(s) 73
BplI GAGNNNNNCTC 4 cut(s) 88, 120, 284, 316
BpuMI CCSGG 2 cut(s) 384, 385
BsaJI CCNNGG 1 cut(s) 383
BsaWI WCCGGW 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 383
BseMII CTCAG 1 cut(s) 224
BseXI GCAGC 5 cut(s) 69, 72, 246, 274, 333
BshFI GGCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 2 cut(s) 384, 413
BsmAI GTCTC 1 cut(s) 241
BsnI GGCC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 101, 303
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 1 cut(s) 223
BspHI TCATGA 3 cut(s) 19, 240, 427
BspMAI CTGCAG 2 cut(s) 264, 332
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 2 cut(s) 101, 303
Bst6I CTCTTC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 1 cut(s) 210
BstKTI GATC 2 cut(s) 104, 306
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 2 cut(s) 101, 303
BstMWI GCNNNNNNNGC 3 cut(s) 91, 327, 373
BstNSI RCATGY 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 382, 383
BstSFI CTRYAG 3 cut(s) 260, 328, 443
BstV1I GCAGC 5 cut(s) 69, 72, 246, 274, 333
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 1 cut(s) 299
Cac8I GCNNGC 1 cut(s) 342
CaiI CAGNNNCTG 2 cut(s) 217, 327
CciI TCATGA 3 cut(s) 19, 240, 427
Cfr13I GGNCC 3 cut(s) 108, 214, 297
Cfr9I CCCGGG 1 cut(s) 383
CpoI CGGWCCG 1 cut(s) 108
Csp6I GTAC 1 cut(s) 113
CspI CGGWCCG 1 cut(s) 108
CviAII CATG 5 cut(s) 20, 181, 221, 241, 428
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 1 cut(s) 210
DpnI GATC 2 cut(s) 103, 305
DpnII GATC 2 cut(s) 101, 303
DrdI GACNNNNNNGTC 1 cut(s) 159
DseDI GACNNNNNNGTC 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 108, 214
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 5 cut(s) 23, 184, 224, 244, 431
FaiI YATR 6 cut(s) 21, 182, 222, 242, 351, 429
FatI CATG 5 cut(s) 19, 180, 220, 240, 427
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 5 cut(s) 83, 86, 260, 263, 322
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 83, 86, 260, 263, 322
FspBI CTAG 1 cut(s) 167
GluI GCNGC 5 cut(s) 83, 86, 260, 263, 322
HaeIII GGCC 1 cut(s) 299
HapII CCGG 2 cut(s) 384, 413
Hin1II CATG 5 cut(s) 23, 184, 224, 244, 431
HindIII AAGCTT 1 cut(s) 274
HinfI GANTC 2 cut(s) 8, 206
HpaII CCGG 2 cut(s) 384, 413
Hpy166II GTNNAC 2 cut(s) 152, 178
Hpy188I TCNGA 2 cut(s) 69, 408
Hpy188III TCNNGA 4 cut(s) 20, 241, 312, 428
Hpy8I GTNNAC 2 cut(s) 152, 178
HpyAV CCTTC 1 cut(s) 408
HpyCH4V TGCA 4 cut(s) 64, 220, 262, 330
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 327, 373
HpyF3I CTNAG 1 cut(s) 210
Hsp92II CATG 5 cut(s) 23, 184, 224, 244, 431
Kzo9I GATC 2 cut(s) 101, 303
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 5 cut(s) 69, 72, 246, 274, 333
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 167
MalI GATC 2 cut(s) 103, 305
MboI GATC 2 cut(s) 101, 303
MboII GAAGA 3 cut(s) 68, 111, 302
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 3 cut(s) 59, 72, 307
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 2 cut(s) 310, 424
MseI TTAA 1 cut(s) 201
MspI CCGG 2 cut(s) 384, 413
MspR9I CCNGG 2 cut(s) 384, 385
MwoI GCNNNNNNNGC 3 cut(s) 91, 327, 373
NciI CCSGG 2 cut(s) 384, 385
NdeII GATC 2 cut(s) 101, 303
NlaIII CATG 5 cut(s) 23, 184, 224, 244, 431
NmeAIII GCCGAG 1 cut(s) 120
NspI RCATGY 1 cut(s) 224
PagI TCATGA 3 cut(s) 19, 240, 427
PfeI GAWTC 2 cut(s) 8, 206
PkrI GCNGC 5 cut(s) 84, 87, 261, 264, 323
PpuMI RGGWCCY 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 214
PspPI GGNCC 3 cut(s) 108, 214, 297
PspPPI RGGWCCY 1 cut(s) 214
PstI CTGCAG 2 cut(s) 264, 332
PstNI CAGNNNCTG 2 cut(s) 217, 327
PsuI RGATCY 1 cut(s) 101
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
Rsr2I CGGWCCG 1 cut(s) 108
RsrII CGGWCCG 1 cut(s) 108
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 5 cut(s) 83, 86, 260, 263, 322
Sau3AI GATC 2 cut(s) 101, 303
Sau96I GGNCC 3 cut(s) 108, 214, 297
ScrFI CCNGG 2 cut(s) 384, 385
SduI GDGCHC 1 cut(s) 342
SetI ASST 8 cut(s) 84, 120, 172, 216, 278, 326, 342, 369
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 3 cut(s) 260, 328, 443
SinI GGWCC 2 cut(s) 108, 214
SmaI CCCGGG 1 cut(s) 385
Sse9I AATT 3 cut(s) 59, 72, 307
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 382, 383
TaqI TCGA 2 cut(s) 140, 302
TaqII GACCGA 1 cut(s) 96
TasI AATT 3 cut(s) 59, 72, 307
TfiI GAWTC 2 cut(s) 8, 206
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 5 cut(s) 82, 85, 259, 262, 321
TspDTI ATGAA 6 cut(s) 8, 36, 197, 229, 303, 416
TspGWI ACGGA 1 cut(s) 100
TspMI CCCGGG 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 108, 214
XceI RCATGY 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmaI CCCGGG 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.