Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0006611

Physical Location & Seq

Genomic Coordinates Forward (+)

20672936 .. 20679007

Gene Structure 6072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG188500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

ATGGTGGATCCCGATTCTTCTTGCTCTGATGCAAATGAAGATTTTGCTCGGGAAAATTGTTATAGCCCAGAAGTTATTAGCACTACAAAGTTCAGGGATGCAGTTTCAAATGACGTTCTTGATGGCCGGGCCATGATAGGATTTTCACTTACATCTCCTGATTTGGTTATGTGTGCCGGGTCACCGGATATACCCCGAACCAGCTGTGCGGAGTCCCCTGAGTTCTTGGAGAGTCGATGCCATCAGAAGCTGGATACTTCGATGGAGCTCTCTTTCGAGAATGGAATCGATGCGTCTCAAGTTAAGGATACTAATAGGACCCCAACTGTGAAGTTCTCCACCACGTTGTGCGAAACCTATACGGAGGATTTGTCTCCTGAAGCTTCATTTGAGCTTCTTCCGCCTACGGTGATTGAGGACAAGTCGAAGGAAGGTCCCCTTCTTGATGTAAACAACAATGTAGGTTCTGCAGATGGCTTCCTGGGCGGTGAATCACCTAAAGATGATGGCAATGCCATGTGTGAAGGAGACTATCAGAAGTTGCTAATCGGTTATAAGAACCAGGGAAAGGAATTAGCAGAGATGAGGAGTGCATTCGAGGAGCTCAAAAAGCAGAACCAATCCAAGAGTAATGAATGCCAAGAAGCTTGGAAGTCTTTAAAGGACCTCCAAAATGAACTCATGCGCAAGTCGATGCATGTTGGATCCTTGGCTTTTGCCATTGAGGGACAGGTGAAAGAGAAAAGCAAGTGGTTTGCATCATTGAGAGATTTGACAAGAAAACTTAAGATTATGAAGATGGACCACATTAAGCTATCAGAGGAGGCATCGGCTTATAAGAACTGTCTTGCAGATATGAACGAGATGAGGTCTACCATTCATTCCACTTTAAATCAGCAAGTAAGTTTGCATAATGATCTGAAGACAAAGTTTATTGAAGGGGCCAAAGAACGTAAAGAACTCTACAACAAGGTGTTAGAGTTGAAAGGAAACATAAGGGTCTTTTGCAGGTGTAGGCCTCTAAACACTGAGGAAGTTTCAGCAGGAGCTTCAATGGCTATTGACTTTGAATCTGAGAAAGATGGTGAGCTTGCTGTCAAGTCAAATGGGGTTACAAGAAGGACCTTTAAGTTTGATGCAGTATTCGGCCCTCAAGCAGACCAAGCTGATGTCTTCGAAGACACAGCTCCATTTGCAACCTCAGTTCTAGATGGGTACAATGTCTGCATATTTGCTTATGGACAGACTGGAAGTGGAAAAACCTTTACAATGGAGGGAACGGAAGAAGCTCGGGGAGTCAATTTTAGGACTCTTGAGCAACTGTTTCGTATAATAAAAGAGCGAGAAAAGTTGCATAGATATGATGTGTCTGTTAGTGTTTTAGAAGTGTACAACGAGCAAATAAGGGATCTACTTGTTCCAGGGAATCAATCAGGAGCAGCTGCCAAAAGGCTTGAAATTAGACAAGCTGGGGAAGGGACACATCATGTTCCAGGACTTGTTGAGGCACGTGTGAAAAACATGAATGAAGTCTGGGAAGTTCTCCAAACTGGCAGTAATGCAAGGGCTGTTGGCTCAACCAATGCCAATGAGCATAGCAGCCGGTCGCACTGCATACACTGTGTAATGGTGAAGGGGGAGAACTTGTTGAATGGGGAATGCACAAGAAGCAAGCTCTGGTTGGTGGATCTAGCAGGGAGTGAGCGTATAGCAAAGACAGAGGTGCAAGGAGAACGACTCAAGGAAACTCAAAATATCAATAGATCTCTATCTGCACTAGGTGATGTCATATCTGCACTTGCAACTAAAACCTCACACATACCCTTCAGGAACTCCAAGCTAACCCACTTGCTTCAAGACTCTCTAGGTGGAGACTCCAAGACGCTTATGTTTCTTCAAATTAGTCCTAATGAAAATGACTTGAGTGAGACCCTTTGCTCACTGAACTTTGCGAGTAGAGTTAGAGGGATAGAGTTGGGTCCTGCAAAGAGACAAATGGACACTTCTGAACTTTTAAGATACAAACAGATGTTTGAGAAAACAAAGCTAGACATCAAGAGCAAAGATGTACAGATAAGGAAGATGGAGGAAACAGTCCATGGGTTAGAACTGAAGATAAGAGAGAGAGACCTGAAAAATAAATGCCTGCAAGACAAGGTGAAAGAATTGGAATCACAACTTTTAATTGAGAGAAAGCTAGCACGTCAGCATGTGGACACAAAAATAGCCGAGCAGCACCTGCAGCAACTGAAACATCAACAAGACGAGCAAACTACTGCACTTGCAAGACCACCTCTAACAAACCGACCATTAACAACTCACAAGATTCTCAATGAGACAGCCATTAATACATTGGGGAAGGACCAAGTAAACCCCACACAACAACTAACTGAGAAGGCCAACAACAAAATTCCAGTACCTACAATGGATGGTTTTGTCAAGTATATTGATCCTATAGAGAAAGAAAACAACCCCGACATGGCTGAACAATTTGTTCTACCAAAGAGAACTGGAAGAGCCTCTATCTGCCCGACAGTTCAGCGGATTCCTGCTACATCTGCTCCAAGACGCAACTCTCTAATTCCACTCCCTACTGTACCAAACCGCCTCCAATCCCCAATGCCTGTATTATCATTAGCACCAATATCAGCATACCATGCTGATAAGAAAGCTGAAATGGATTATGTGTCTGAAATTGACTGCTTGCCTGAACAGACACCATGTAGTAGTCCTAAAGTTACCAGAACTGGTGGCAAGAAATTCAACAGCATACTGAGACGAAGCATCCAAAAGAAAGCTCAGATGAAGTCCCCAATGCCTCCACACATGAGAAAAGGTGTGAATGTAGGGATGGAGAAGGTTAGAGTATCTATTGGAAGTCGAGGCAGGATGCCACACAGGGTGTTACTTGGAAATGGTAGAAGGGCAGGTACTAAAGAAGGTCAGAAGCAGATTAGGGAAAAGGAGAGGGGGTGGAACATTGGTACAGCAGGGAGAAATGCTATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Malectin

Protein Analysis

1002

Amino Acids

112.04

Weight (kDa)

8.38

Isoelectric Point (pI)

43.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	315 - 472	9.2e-46	Microtubule binding
Kinesin	PF00225	337 - 657	2.7e-116	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006611)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72250 AT1G72250 AT1G72250 AT1G72250
fragaria_vesca	FvH4_3g16110 FvH4_3g16110 FvH4_3g16110
malus_domestica	MD05G1213600.v1.1 MD10G1197400.v1.1
prunus_persica	Prupe.4G144400_v2.0.a1 Prupe.4G144400_v2.0.a1
pyrus_communis	pycom05g19730 pycom10g17030
rosa_chinensis	RchiOBHm_Chr5g0027091
rosa_laevigata	RLG00000032989 RLG00000032991
rosa_multiflora	Rmu_sc0000882.1_g000027 Rmu_sc0006065.1_g000024
rosa_roxburghii	Rroxscaffold_1G00052400
rosa_rugosa	Rorug05G0097200 Rorug05G0097200
rosa_samantha	Rh5AG190900 Rh5BG188500 Rh5CG208300 Rh5DG190300
rosa_wichuraiana	Rw5G017390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	555, 837
AarI	CACCTGC	2 cut(s)	999, 2247
Acc16I	TGCGCA	1 cut(s)	686
Acc36I	ACCTGC	3 cut(s)	999, 2247, 2918
AccI	GTMKAC	1 cut(s)	872
AciI	CCGC	5 cut(s)	209, 401, 486, 2542, 2605
AclWI	GGATC	7 cut(s)	2, 15, 699, 712, 1416, 1695, 2444
AcoI	YGGCCR	1 cut(s)	124
AcsI	RAATTY	2 cut(s)	2409, 2759
AcuI	CTGAAG	4 cut(s)	399, 941, 1810, 2132
AcvI	CACGTG	1 cut(s)	1511
AdeI	CACNNNGTG	3 cut(s)	348, 1781, 2902
AfaI	GTAC	7 cut(s)	1217, 1391, 2070, 2418, 2598, 2932, 2986
AfiI	CCNNNNNNNGG	2 cut(s)	568, 2479
AflII	CTTAAG	1 cut(s)	785
AflIII	ACRYGT	1 cut(s)	1510
AjiI	CACGTC	1 cut(s)	2204
AjnI	CCWGG	4 cut(s)	480, 561, 1420, 1492
Alw21I	GWGCWC	2 cut(s)	270, 606
Alw26I	GTCTC	9 cut(s)	300, 378, 522, 1866, 1922, 1984, 2121, 2330, 2770
AlwI	GGATC	7 cut(s)	2, 15, 699, 712, 1416, 1695, 2444
AlwNI	CAGNNNCTG	3 cut(s)	250, 2239, 2248
Ama87I	CYCGRG	2 cut(s)	48, 1290
AoxI	GGCC	6 cut(s)	124, 129, 942, 1016, 1147, 2397
ApeKI	GCWGC	5 cut(s)	1439, 1442, 1599, 2233, 2242
ApoI	RAATTY	2 cut(s)	2409, 2759
AseI	ATTAAT	1 cut(s)	2346
AspLEI	GCGC	1 cut(s)	687
AsuC2I	CCSGG	2 cut(s)	128, 178
AsuHPI	GGTGA	9 cut(s)	174, 421, 486, 500, 745, 1097, 1642, 1793, 2170
AsuII	TTCGAA	1 cut(s)	1176
AsuNHI	GCTAGC	1 cut(s)	2197
AvaI	CYCGRG	2 cut(s)	48, 1290
AvaII	GGWCC	7 cut(s)	318, 434, 664, 802, 1122, 1979, 2362
BamHI	GGATCC	2 cut(s)	7, 704
BanII	GRGCYC	2 cut(s)	270, 606
BarI	GAAGNNNNNNTAC	2 cut(s)	2914, 2946
BbrPI	CACGTG	1 cut(s)	1511
BbsI	GAAGAC	3 cut(s)	929, 1165, 1185
Bbv12I	GWGCWC	2 cut(s)	270, 606
BbvI	GCAGC	5 cut(s)	1429, 1451, 1611, 2245, 2254
BcgI	CGANNNNNNTGC	2 cut(s)	1307, 1341
BciT130I	CCWGG	4 cut(s)	482, 563, 1422, 1494
BciVI	GTATCC	2 cut(s)	247, 301
BcnI	CCSGG	2 cut(s)	128, 178
BcoDI	GTCTC	9 cut(s)	300, 378, 522, 1866, 1922, 1984, 2121, 2330, 2770
BfaI	CTAG	6 cut(s)	1208, 1691, 1778, 1865, 2048, 2198
BfmI	CTRYAG	3 cut(s)	468, 2240, 2454
BfrI	CTTAAG	1 cut(s)	785
BfuAI	ACCTGC	3 cut(s)	999, 2247, 2918
BfuI	GTATCC	2 cut(s)	247, 301
BglII	AGATCT	1 cut(s)	1763
BisI	GCNGC	5 cut(s)	1440, 1443, 1600, 2234, 2243
BlsI	GCNGC	5 cut(s)	1441, 1444, 1601, 2235, 2244
Bme1390I	CCNGG	6 cut(s)	128, 178, 482, 563, 1422, 1494
Bme18I	GGWCC	7 cut(s)	318, 434, 664, 802, 1122, 1979, 2362
BmeT110I	CYCGRG	2 cut(s)	48, 1290
BmgBI	CACGTC	1 cut(s)	2204
BmiI	GGNNCC	6 cut(s)	9, 320, 436, 706, 943, 1980
BmrFI	CCNGG	6 cut(s)	128, 178, 482, 563, 1422, 1494
BmtI	GCTAGC	1 cut(s)	2201
BpiI	GAAGAC	3 cut(s)	929, 1165, 1185
BplI	GAGNNNNNCTC	2 cut(s)	1722, 1754
Bpu14I	TTCGAA	1 cut(s)	1176
BpuEI	CTTGAG	5 cut(s)	282, 1137, 1334, 1724, 1942
BpuMI	CCSGG	2 cut(s)	128, 178
Bsa29I	ATCGAT	1 cut(s)	288
BsaAI	YACGTR	1 cut(s)	1511
BsaBI	GATNNNNATC	1 cut(s)	1768
BsaI	GGTCTC	2 cut(s)	1922, 2121
BsaJI	CCNNGG	5 cut(s)	481, 562, 708, 1421, 2098
BsaWI	WCCGGW	1 cut(s)	184
Bsc4I	CCNNNNNNNGG	2 cut(s)	568, 2479
Bse118I	RCCGGY	1 cut(s)	1602
Bse1I	ACTGG	5 cut(s)	1252, 1555, 2414, 2515, 2752
Bse3DI	GCAATG	1 cut(s)	517
Bse8I	GATNNNNATC	1 cut(s)	1768
BseBI	CCWGG	4 cut(s)	482, 563, 1422, 1494
BseCI	ATCGAT	1 cut(s)	288
BseDI	CCNNGG	5 cut(s)	481, 562, 708, 1421, 2098
BseGI	GGATG	5 cut(s)	103, 2434, 2784, 2856, 2895
BseJI	GATNNNNATC	1 cut(s)	1768
BseLI	CCNNNNNNNGG	2 cut(s)	568, 2479
BseMI	GCAATG	1 cut(s)	517
BseMII	CTCAG	7 cut(s)	210, 1020, 1065, 1215, 2382, 2765, 2813
BseNI	ACTGG	5 cut(s)	1252, 1555, 2414, 2515, 2752
BseRI	GAGGAG	3 cut(s)	601, 614, 836
BseXI	GCAGC	5 cut(s)	1429, 1451, 1611, 2245, 2254
BseYI	CCCAGC	1 cut(s)	1469
BsgI	GTGCAG	3 cut(s)	1758, 1779, 2262
Bsh1285I	CGRYCG	1 cut(s)	1607
BshFI	GGCC	6 cut(s)	126, 131, 944, 1018, 1149, 2399
BshVI	ATCGAT	1 cut(s)	288
BsiEI	CGRYCG	1 cut(s)	1607
BsiHKAI	GWGCWC	2 cut(s)	270, 606
BsiHKCI	CYCGRG	2 cut(s)	48, 1290
BsiSI	CCGG	4 cut(s)	127, 177, 185, 1603
BslFI	GGGAC	5 cut(s)	199, 420, 741, 1492, 2794
BslI	CCNNNNNNNGG	2 cut(s)	568, 2479
BsmAI	GTCTC	9 cut(s)	300, 378, 522, 1866, 1922, 1984, 2121, 2330, 2770
BsmBI	CGTCTC	2 cut(s)	300, 2770
BsmFI	GGGAC	5 cut(s)	199, 420, 741, 1492, 2794
BsmI	GAATGC	3 cut(s)	593, 641, 1664
BsnI	GGCC	6 cut(s)	126, 131, 944, 1018, 1149, 2399
Bso31I	GGTCTC	2 cut(s)	1922, 2121
BsoBI	CYCGRG	2 cut(s)	48, 1290
Bsp119I	TTCGAA	1 cut(s)	1176
Bsp1286I	GDGCHC	2 cut(s)	270, 606
Bsp1407I	TGTACA	2 cut(s)	1389, 2068
Bsp143I	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
Bsp19I	CCATGG	1 cut(s)	2098
BspACI	CCGC	5 cut(s)	209, 401, 486, 2542, 2605
BspANI	GGCC	6 cut(s)	126, 131, 944, 1018, 1149, 2399
BspCNI	CTCAG	7 cut(s)	211, 1021, 1066, 1214, 2383, 2766, 2812
BspDI	ATCGAT	1 cut(s)	288
BspLI	GGNNCC	6 cut(s)	9, 320, 436, 706, 943, 1980
BspMAI	CTGCAG	2 cut(s)	472, 2244
BspMI	ACCTGC	3 cut(s)	999, 2247, 2918
BspOI	GCTAGC	1 cut(s)	2201
BspPI	GGATC	7 cut(s)	2, 15, 699, 712, 1416, 1695, 2444
BspQI	GCTCTTC	1 cut(s)	2509
BspT104I	TTCGAA	1 cut(s)	1176
BspTI	CTTAAG	1 cut(s)	785
BspTNI	GGTCTC	2 cut(s)	1922, 2121
BsrDI	GCAATG	1 cut(s)	517
BsrFI	RCCGGY	1 cut(s)	1602
BsrGI	TGTACA	2 cut(s)	1389, 2068
BsrI	ACTGG	5 cut(s)	1252, 1555, 2414, 2515, 2752
BssAI	RCCGGY	1 cut(s)	1602
BssECI	CCNNGG	5 cut(s)	481, 562, 708, 1421, 2098
BssMI	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
BssT1I	CCWWGG	2 cut(s)	708, 2098
Bst2UI	CCWGG	4 cut(s)	482, 563, 1422, 1494
Bst4CI	ACNGT	8 cut(s)	328, 409, 845, 1323, 1622, 2095, 2536, 2596
Bst6I	CTCTTC	1 cut(s)	2509
BstAFI	CTTAAG	1 cut(s)	785
BstAPI	GCANNNNNTGC	2 cut(s)	2239, 2657
BstAUI	TGTACA	2 cut(s)	1389, 2068
BstBAI	YACGTR	1 cut(s)	1511
BstBI	TTCGAA	1 cut(s)	1176
BstC8I	GCNNGC	5 cut(s)	1092, 1673, 2147, 2199, 2705
BstDEI	CTNAG	7 cut(s)	219, 1029, 1074, 1201, 2391, 2774, 2799
BstDSI	CCRYGG	1 cut(s)	2098
BstEII	GGTNACC	1 cut(s)	180
BstF5I	GGATG	5 cut(s)	103, 2434, 2784, 2856, 2895
BstHHI	GCGC	1 cut(s)	687
BstKTI	GATC	7 cut(s)	10, 707, 919, 1411, 1690, 1766, 2452
BstMAI	GTCTC	9 cut(s)	300, 378, 522, 1866, 1922, 1984, 2121, 2330, 2770
BstMBI	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
BstMCI	CGRYCG	1 cut(s)	1607
BstNI	CCWGG	4 cut(s)	482, 563, 1422, 1494
BstNSI	RCATGY	2 cut(s)	701, 2213
BstPI	GGTNACC	1 cut(s)	180
BstSCI	CCNGG	6 cut(s)	126, 176, 480, 561, 1420, 1492
BstSFI	CTRYAG	3 cut(s)	468, 2240, 2454
BstV1I	GCAGC	5 cut(s)	1429, 1451, 1611, 2245, 2254
BstV2I	GAAGAC	3 cut(s)	929, 1165, 1185
BstX2I	RGATCY	5 cut(s)	7, 704, 1408, 1687, 1763
BstYI	RGATCY	5 cut(s)	7, 704, 1408, 1687, 1763
Bsu15I	ATCGAT	1 cut(s)	288
BsuI	GTATCC	2 cut(s)	247, 301
BsuRI	GGCC	6 cut(s)	126, 131, 944, 1018, 1149, 2399
BsuTUI	ATCGAT	1 cut(s)	288
BtgI	CCRYGG	1 cut(s)	2098
BtrI	CACGTC	1 cut(s)	2204
BtsCI	GGATG	5 cut(s)	103, 2434, 2784, 2856, 2895
BtsI	GCAGTG	1 cut(s)	1609
BtsIMutI	CAGTG	4 cut(s)	1026, 1609, 1618, 1940
BveI	ACCTGC	3 cut(s)	999, 2247, 2918
Cac8I	GCNNGC	5 cut(s)	1092, 1673, 2147, 2199, 2705
CaiI	CAGNNNCTG	3 cut(s)	250, 2239, 2248
CfoI	GCGC	1 cut(s)	687
Cfr10I	RCCGGY	1 cut(s)	1602
ClaI	ATCGAT	1 cut(s)	288
CseI	GACGC	3 cut(s)	282, 1891, 2577
Csp6I	GTAC	7 cut(s)	1216, 1390, 2069, 2417, 2597, 2931, 2985
CviQI	GTAC	7 cut(s)	1216, 1390, 2069, 2417, 2597, 2931, 2985
DdeI	CTNAG	7 cut(s)	219, 1029, 1074, 1201, 2391, 2774, 2799
DpnI	GATC	7 cut(s)	9, 706, 918, 1410, 1689, 1765, 2451
DpnII	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
DraI	TTTAAA	2 cut(s)	660, 891
DraIII	CACNNNGTG	3 cut(s)	348, 1781, 2902
EaeI	YGGCCR	1 cut(s)	124
Eam1104I	CTCTTC	1 cut(s)	2509
EarI	CTCTTC	1 cut(s)	2509
EciI	GGCGGA	1 cut(s)	390
Ecl136II	GAGCTC	2 cut(s)	268, 604
Eco130I	CCWWGG	2 cut(s)	708, 2098
Eco147I	AGGCCT	1 cut(s)	1018
Eco24I	GRGCYC	2 cut(s)	270, 606
Eco31I	GGTCTC	2 cut(s)	1922, 2121
Eco47I	GGWCC	7 cut(s)	318, 434, 664, 802, 1122, 1979, 2362
Eco53kI	GAGCTC	2 cut(s)	268, 604
Eco57I	CTGAAG	4 cut(s)	399, 941, 1810, 2132
Eco72I	CACGTG	1 cut(s)	1511
Eco88I	CYCGRG	2 cut(s)	48, 1290
Eco91I	GGTNACC	1 cut(s)	180
EcoICRI	GAGCTC	2 cut(s)	268, 604
EcoO109I	RGGNCCY	5 cut(s)	318, 434, 664, 1122, 1979
EcoO65I	GGTNACC	1 cut(s)	180
EcoRII	CCWGG	4 cut(s)	480, 561, 1420, 1492
EcoT14I	CCWWGG	2 cut(s)	708, 2098
EcoT22I	ATGCAT	1 cut(s)	699
EcoT38I	GRGCYC	2 cut(s)	270, 606
ErhI	CCWWGG	2 cut(s)	708, 2098
Esp3I	CGTCTC	2 cut(s)	300, 2770
FalI	AAGNNNNNCTT	2 cut(s)	423, 455
FaqI	GGGAC	5 cut(s)	199, 420, 741, 1492, 2794
FblI	GTMKAC	1 cut(s)	872
Fnu4HI	GCNGC	5 cut(s)	1440, 1443, 1600, 2234, 2243
FokI	GGATG	5 cut(s)	110, 2441, 2771, 2863, 2902
FriOI	GRGCYC	2 cut(s)	270, 606
Fsp4HI	GCNGC	5 cut(s)	1440, 1443, 1600, 2234, 2243
FspBI	CTAG	6 cut(s)	1208, 1691, 1778, 1865, 2048, 2198
FspI	TGCGCA	1 cut(s)	686
GlaI	GCGC	1 cut(s)	686
GluI	GCNGC	5 cut(s)	1440, 1443, 1600, 2234, 2243
GsaI	CCCAGC	1 cut(s)	1473
HaeIII	GGCC	6 cut(s)	126, 131, 944, 1018, 1149, 2399
HapII	CCGG	4 cut(s)	127, 177, 185, 1603
HgaI	GACGC	3 cut(s)	282, 1891, 2577
HhaI	GCGC	1 cut(s)	687
Hin6I	GCGC	1 cut(s)	685
HinP1I	GCGC	1 cut(s)	685
HindIII	AAGCTT	2 cut(s)	381, 645
HpaII	CCGG	4 cut(s)	127, 177, 185, 1603
HphI	GGTGA	9 cut(s)	174, 421, 486, 500, 745, 1097, 1642, 1793, 2170
Hpy166II	GTNNAC	5 cut(s)	451, 873, 1390, 2215, 2371
Hpy8I	GTNNAC	5 cut(s)	451, 873, 1390, 2215, 2371
HpyCH4III	ACNGT	8 cut(s)	328, 409, 845, 1323, 1622, 2095, 2536, 2596
HpyCH4IV	ACGT	5 cut(s)	114, 344, 952, 1510, 2203
HpyF3I	CTNAG	7 cut(s)	219, 1029, 1074, 1201, 2391, 2774, 2799
HpySE526I	ACGT	5 cut(s)	114, 344, 952, 1510, 2203
HspAI	GCGC	1 cut(s)	685
Kzo9I	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
LguI	GCTCTTC	1 cut(s)	2509
LmnI	GCTCC	6 cut(s)	265, 601, 1046, 1192, 1436, 2566
Lsp1109I	GCAGC	5 cut(s)	1429, 1451, 1611, 2245, 2254
MaeI	CTAG	6 cut(s)	1208, 1691, 1778, 1865, 2048, 2198
MaeII	ACGT	5 cut(s)	114, 344, 952, 1510, 2203
MaeIII	GTNAC	4 cut(s)	180, 1111, 2737, 2904
MalI	GATC	7 cut(s)	9, 706, 918, 1410, 1689, 1765, 2451
MboI	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
MflI	RGATCY	5 cut(s)	7, 704, 1408, 1687, 1763
MhlI	GDGCHC	2 cut(s)	270, 606
MlyI	GAGTC	7 cut(s)	221, 241, 1303, 1305, 1731, 1853, 1868
MmeI	TCCRAC	1 cut(s)	682
Mph1103I	ATGCAT	1 cut(s)	699
MslI	CAYNNNNRTG	1 cut(s)	1359
MspA1I	CMGCKG	3 cut(s)	204, 1442, 2542
MspCI	CTTAAG	1 cut(s)	785
MspI	CCGG	4 cut(s)	127, 177, 185, 1603
MspR9I	CCNGG	6 cut(s)	128, 178, 482, 563, 1422, 1494
Mva1269I	GAATGC	3 cut(s)	593, 641, 1664
MvaI	CCWGG	4 cut(s)	482, 563, 1422, 1494
NciI	CCSGG	2 cut(s)	128, 178
NcoI	CCATGG	1 cut(s)	2098
NdeII	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
NheI	GCTAGC	1 cut(s)	2197
NlaIV	GGNNCC	6 cut(s)	9, 320, 436, 706, 943, 1980
NmeAIII	GCCGAG	1 cut(s)	2254
NmuCI	GTSAC	1 cut(s)	180
NsbI	TGCGCA	1 cut(s)	686
NsiI	ATGCAT	1 cut(s)	699
NspI	RCATGY	2 cut(s)	701, 2213
NspV	TTCGAA	1 cut(s)	1176
PaqCI	CACCTGC	2 cut(s)	999, 2247
PceI	AGGCCT	1 cut(s)	1018
PciSI	GCTCTTC	1 cut(s)	2509
PctI	GAATGC	3 cut(s)	593, 641, 1664
PfeI	GAWTC	8 cut(s)	14, 285, 491, 1070, 1426, 2171, 2326, 2545
PfoI	TCCNGGA	1 cut(s)	1492
PkrI	GCNGC	5 cut(s)	1441, 1444, 1601, 2235, 2244
PleI	GAGTC	7 cut(s)	220, 240, 1303, 1304, 1731, 1853, 1868
PmaCI	CACGTG	1 cut(s)	1511
PmlI	CACGTG	1 cut(s)	1511
PpsI	GAGTC	7 cut(s)	220, 240, 1303, 1304, 1731, 1853, 1868
Ppu21I	YACGTR	1 cut(s)	1511
PpuMI	RGGWCCY	5 cut(s)	318, 434, 664, 1122, 1979
PshBI	ATTAAT	1 cut(s)	2346
PsiI	TTATAA	2 cut(s)	555, 837
Psp124BI	GAGCTC	2 cut(s)	270, 606
Psp5II	RGGWCCY	5 cut(s)	318, 434, 664, 1122, 1979
Psp6I	CCWGG	4 cut(s)	480, 561, 1420, 1492
PspCI	CACGTG	1 cut(s)	1511
PspEI	GGTNACC	1 cut(s)	180
PspFI	CCCAGC	1 cut(s)	1469
PspGI	CCWGG	4 cut(s)	480, 561, 1420, 1492
PspN4I	GGNNCC	6 cut(s)	9, 320, 436, 706, 943, 1980
PspPPI	RGGWCCY	5 cut(s)	318, 434, 664, 1122, 1979
PstI	CTGCAG	2 cut(s)	472, 2244
PstNI	CAGNNNCTG	3 cut(s)	250, 2239, 2248
PsuI	RGATCY	5 cut(s)	7, 704, 1408, 1687, 1763
PvuII	CAGCTG	2 cut(s)	204, 1442
RsaI	GTAC	7 cut(s)	1217, 1391, 2070, 2418, 2598, 2932, 2986
RsaNI	GTAC	7 cut(s)	1216, 1390, 2069, 2417, 2597, 2931, 2985
RseI	CAYNNNNRTG	1 cut(s)	1359
SacI	GAGCTC	2 cut(s)	270, 606
SapI	GCTCTTC	1 cut(s)	2509
SatI	GCNGC	5 cut(s)	1440, 1443, 1600, 2234, 2243
Sau3AI	GATC	7 cut(s)	7, 704, 916, 1408, 1687, 1763, 2449
SchI	GAGTC	7 cut(s)	221, 241, 1303, 1305, 1731, 1853, 1868
ScrFI	CCNGG	6 cut(s)	128, 178, 482, 563, 1422, 1494
SduI	GDGCHC	2 cut(s)	270, 606
SfcI	CTRYAG	3 cut(s)	468, 2240, 2454
SfuI	TTCGAA	1 cut(s)	1176
SinI	GGWCC	7 cut(s)	318, 434, 664, 802, 1122, 1979, 2362
SmiMI	CAYNNNNRTG	1 cut(s)	1359
SmlI	CTYRAG	6 cut(s)	297, 785, 1152, 1313, 1739, 1921
SmoI	CTYRAG	6 cut(s)	297, 785, 1152, 1313, 1739, 1921
SseBI	AGGCCT	1 cut(s)	1018
SsiI	CCGC	5 cut(s)	209, 401, 486, 2542, 2605
SspMI	CTAG	6 cut(s)	1208, 1691, 1778, 1865, 2048, 2198
SstI	GAGCTC	2 cut(s)	270, 606
StuI	AGGCCT	1 cut(s)	1018
StyD4I	CCNGG	6 cut(s)	126, 176, 480, 561, 1420, 1492
StyI	CCWWGG	2 cut(s)	708, 2098
TaaI	ACNGT	8 cut(s)	328, 409, 845, 1323, 1622, 2095, 2536, 2596
TaiI	ACGT	5 cut(s)	117, 347, 955, 1513, 2206
TaqI	TCGA	9 cut(s)	235, 260, 276, 288, 425, 597, 692, 1176, 2881
TatI	WGTACW	2 cut(s)	1389, 2068
TfiI	GAWTC	8 cut(s)	14, 285, 491, 1070, 1426, 2171, 2326, 2545
TscAI	CASTG	4 cut(s)	1033, 1616, 1625, 1947
TseFI	GTSAC	1 cut(s)	180
TseI	GCWGC	5 cut(s)	1439, 1442, 1599, 2233, 2242
Tsp45I	GTSAC	1 cut(s)	180
TspGWI	ACGGA	2 cut(s)	377, 1295
TspRI	CASTG	4 cut(s)	1033, 1616, 1625, 1947
Vha464I	CTTAAG	1 cut(s)	785
VpaK11BI	GGWCC	7 cut(s)	318, 434, 664, 802, 1122, 1979, 2362
VspI	ATTAAT	1 cut(s)	2346
XapI	RAATTY	2 cut(s)	2409, 2759
XbaI	TCTAGA	1 cut(s)	1207
XceI	RCATGY	2 cut(s)	701, 2213
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2350
XmiI	GTMKAC	1 cut(s)	872
XspI	CTAG	6 cut(s)	1208, 1691, 1778, 1865, 2048, 2198
Zsp2I	ATGCAT	1 cut(s)	699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.