Rh5BG199900

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020461
Physical Location & Seq
Forward (+)
22306813 .. 22318508
11696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG199900.1

Sequence Viewer

Length: 648 bp
ATGGAAGTGATCAAGTATTTGTTAACAAATGGTCGAGTTTTGGAGAAGATGACTATTTATACTCCTGATGGCGGTGATGGCAATTCTATGGCTTCACGCTTGACAAGAAGACGGGATCGGCGGCCTTCTGCATCTGATCTGGACGAATACAGTTGTGGGTGGGGGTATGCGGCTGTGCTTATGGGTTTTGGTGGGCAGTTTGTCAGAGAAGGGATTGATTCCTCAGAGATGGAGTCTCGCATCATACCTGGGATTGGATTGTCCCATCGGTGGTCTAGATCTTTTGGAAGGGACGACGGTGCTGTTTTCTGGTCGGTGATGATGGTTAGCGATTATCCCGTGGCAGCTGTTTGGGACAAGGATGTAGAATTAGCGTCGAAGAAGATGAGTTTTGAAATTGAAGAAGAGAAAGTACAATGGGGGCTAGTTATAGCGTTAGGAGCAAAGCACATAGGATTTGAAGTACTACAAGTGTTTGAGAAGGATGTGAGAGTTGTGAAGGGGAGGACGTGCTTGAAGCCATTGATGAGATTGTGTGAAGCAGGTTGTGTTACTGGAAATAAGATCAATGGCTTTGCAGACGGCTCTCATCAAAATAATTTACTTCAAAATTCGAAAGTAAGACACAATACAAGTGCTCAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

215

Amino Acids

24.0

Weight (kDa)

8.32

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020461)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0324891
rosa_multiflora Rmu_sc0001575.1_g000014
rosa_samantha Rh2AG433500 Rh5AG201700 Rh5BG199900 Rh5CG220200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 533
AciI CCGC 3 cut(s) 72, 121, 170
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 1 cut(s) 610
AfaI GTAC 2 cut(s) 414, 465
AfiI CCNNNNNNNGG 3 cut(s) 71, 254, 270
AgsI TTSAA 5 cut(s) 395, 401, 461, 517, 608
AjiI CACGTC 1 cut(s) 510
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 1 cut(s) 123
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 610
AsuHPI GGTGA 2 cut(s) 86, 328
AsuII TTCGAA 1 cut(s) 614
BarI GAAGNNNNNNTAC 2 cut(s) 396, 428
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 1 cut(s) 356
BccI CCATC 5 cut(s) 62, 71, 223, 273, 316
BceAI ACGGC 1 cut(s) 598
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 2 cut(s) 276, 425
BfuAI ACCTGC 1 cut(s) 533
BglII AGATCT 1 cut(s) 278
BisI GCNGC 3 cut(s) 122, 171, 345
BlsI GCNGC 3 cut(s) 123, 172, 346
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 510
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 140, 249
BpiI GAAGAC 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 248, 339
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 254, 270
Bse1I ACTGG 1 cut(s) 559
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 248, 339
BseGI GGATG 2 cut(s) 367, 490
BseLI CCNNNNNNNGG 3 cut(s) 71, 254, 270
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 559
BseXI GCAGC 1 cut(s) 356
BshFI GGCC 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 640
BslFI GGGAC 3 cut(s) 247, 305, 368
BslI CCNNNNNNNGG 3 cut(s) 71, 254, 270
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 3 cut(s) 247, 305, 368
BsnI GGCC 1 cut(s) 124
Bsp119I TTCGAA 1 cut(s) 614
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 5 cut(s) 9, 115, 136, 278, 564
BspACI CCGC 3 cut(s) 72, 121, 170
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 1 cut(s) 236
BspMI ACCTGC 1 cut(s) 533
BspPI GGATC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 614
BsrI ACTGG 1 cut(s) 559
BssECI CCNNGG 2 cut(s) 248, 339
BssMI GATC 5 cut(s) 9, 115, 136, 278, 564
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 152, 299
Bst6I CTCTTC 1 cut(s) 399
BstBI TTCGAA 1 cut(s) 614
BstDEI CTNAG 1 cut(s) 223
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 367, 490
BstKTI GATC 5 cut(s) 12, 118, 139, 281, 567
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 5 cut(s) 9, 115, 136, 278, 564
BstMWI GCNNNNNNNGC 2 cut(s) 78, 440
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 356
BstV2I GAAGAC 1 cut(s) 115
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 1 cut(s) 124
BtgI CCRYGG 1 cut(s) 339
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 2 cut(s) 367, 490
BveI ACCTGC 1 cut(s) 533
CseI GACGC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 413, 464
CviJI RGCY 8 cut(s) 92, 124, 173, 347, 424, 520, 573, 585
CviKI_1 RGCY 8 cut(s) 92, 124, 173, 347, 424, 520, 573, 585
CviQI GTAC 2 cut(s) 413, 464
DdeI CTNAG 1 cut(s) 223
DpnI GATC 5 cut(s) 11, 117, 138, 280, 566
DpnII GATC 5 cut(s) 9, 115, 136, 278, 564
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 247
FaiI YATR 7 cut(s) 60, 89, 168, 182, 245, 431, 452
FaqI GGGAC 3 cut(s) 247, 305, 368
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 122, 171, 345
FokI GGATG 2 cut(s) 374, 497
Fsp4HI GCNGC 3 cut(s) 122, 171, 345
FspBI CTAG 2 cut(s) 276, 425
GluI GCNGC 3 cut(s) 122, 171, 345
HaeIII GGCC 1 cut(s) 124
HgaI GACGC 1 cut(s) 363
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 218, 233
HpaI GTTAAC 1 cut(s) 24
HphI GGTGA 2 cut(s) 86, 328
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 136, 206, 226
Hpy188III TCNNGA 3 cut(s) 65, 140, 276
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 2 cut(s) 299, 379
HpyAV CCTTC 5 cut(s) 135, 203, 282, 475, 493
HpyCH4III ACNGT 2 cut(s) 152, 299
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 2 cut(s) 131, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 440
HpyF3I CTNAG 1 cut(s) 223
HpySE526I ACGT 1 cut(s) 509
Ksp22I TGATCA 1 cut(s) 9
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 5 cut(s) 9, 115, 136, 278, 564
LmnI GCTCC 1 cut(s) 440
LpnPI CCDG 7 cut(s) 78, 125, 234, 261, 295, 528, 540
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 2 cut(s) 140, 249
MaeI CTAG 2 cut(s) 276, 425
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 1 cut(s) 550
MalI GATC 5 cut(s) 11, 117, 138, 280, 566
MboI GATC 5 cut(s) 9, 115, 136, 278, 564
MboII GAAGA 6 cut(s) 58, 120, 391, 394, 413, 416
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 1 cut(s) 640
MluCI AATT 5 cut(s) 82, 368, 396, 598, 610
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 2 cut(s) 232, 498
MseI TTAA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 78, 440
NdeII GATC 5 cut(s) 9, 115, 136, 278, 564
NspV TTCGAA 1 cut(s) 614
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 3 cut(s) 123, 172, 346
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 414, 465
RsaNI GTAC 2 cut(s) 413, 464
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 3 cut(s) 122, 171, 345
Sau3AI GATC 5 cut(s) 9, 115, 136, 278, 564
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 640
SetI ASST 4 cut(s) 250, 349, 512, 547
SfaNI GCATC 2 cut(s) 140, 249
SfuI TTCGAA 1 cut(s) 614
Sse9I AATT 5 cut(s) 82, 368, 396, 598, 610
SsiI CCGC 3 cut(s) 72, 121, 170
SspMI CTAG 2 cut(s) 276, 425
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 152, 299
TaiI ACGT 1 cut(s) 512
TaqI TCGA 3 cut(s) 34, 377, 614
TasI AATT 5 cut(s) 82, 368, 396, 598, 610
TatI WGTACW 2 cut(s) 412, 463
TauI GCSGC 2 cut(s) 124, 173
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TseI GCWGC 1 cut(s) 344
XapI RAATTY 1 cut(s) 610
XbaI TCTAGA 1 cut(s) 275
XspI CTAG 2 cut(s) 276, 425
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.