Rh5BG378400

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0022553
Physical Location & Seq
Reverse (-)
59921657 .. 59922025
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG378400.1

Sequence Viewer

Length: 369 bp
ATGTCCGGACGCGGCGGCCGAGGCCGGCATTATTCCGGCGCTCTCTCTAATCCATCGCAGGGCGGAGGTGGAGGCGGCGGAGGAAGTTCAGGCGCCGGGACTCAGTCTCTTGCTTATTCTCCACCAGCAGCCTCGTCAAGTTCGGCTCCTCCGGTGGCTTCGCTGAGTAGCGAGATGGAGCAGAAGTTGACACTCGAGACGACGACGGACGTGGCTGTCGTGCCGCCGGCGTCGTCGAAGGCGGTGAGCTTTCCGCAGAGGCCGAATTTCGGGACGGTCGGCAAGAAAATTCAAGTCAGAGCCAATCACTTCCTGGTGCAGGTTGCAGACAGAGACGTTCACCATTACAATGTAAGGCTTTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

122

Amino Acids

12.48

Weight (kDa)

10.1

Isoelectric Point (pI)

58.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022553)

Species Orthologous Gene IDs
pyrus_communis pycom11g12480
rosa_samantha Rh5BG378400 Rh5BG379000 Rh7BG249500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 5
AciI CCGC 8 cut(s) 12, 15, 63, 75, 78, 224, 242, 254
AcoI YGGCCR 1 cut(s) 16
AcsI RAATTY 2 cut(s) 265, 288
AcyI GRCGYC 2 cut(s) 93, 230
AfiI CCNNNNNNNGG 3 cut(s) 59, 269, 319
AgsI TTSAA 1 cut(s) 293
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw26I GTCTC 3 cut(s) 111, 191, 327
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 3 cut(s) 16, 22, 260
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 265, 288
AspLEI GCGC 2 cut(s) 41, 95
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 2 cut(s) 256, 332
AvaI CYCGRG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 61, 169
BciT130I CCWGG 1 cut(s) 314
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 111, 191, 327
BfoI RGCGCY 2 cut(s) 42, 96
BfuAI ACCTGC 1 cut(s) 310
BisI GCNGC 5 cut(s) 13, 16, 76, 129, 224
BlsI GCNGC 5 cut(s) 14, 17, 77, 130, 225
Bme1390I CCNGG 2 cut(s) 97, 314
BmeT110I CYCGRG 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 94, 147
BmrFI CCNGG 2 cut(s) 97, 314
BpuMI CCSGG 1 cut(s) 97
BsaHI GRCGYC 2 cut(s) 93, 230
BsaJI CCNNGG 1 cut(s) 19
BsaWI WCCGGW 2 cut(s) 5, 151
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 269, 319
Bse118I RCCGGY 2 cut(s) 24, 226
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 19
BseLI CCNNNNNNNGG 3 cut(s) 59, 269, 319
BseMII CTCAG 2 cut(s) 116, 155
BseRI GAGGAG 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 16
BseXI GCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 2 cut(s) 19, 279
BshFI GGCC 3 cut(s) 18, 24, 262
BshNI GGYRCC 1 cut(s) 92
BsiEI CGRYCG 2 cut(s) 19, 279
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 6 cut(s) 6, 25, 36, 96, 152, 227
BslFI GGGAC 2 cut(s) 112, 286
BslI CCNNNNNNNGG 3 cut(s) 59, 269, 319
BsmAI GTCTC 3 cut(s) 111, 191, 327
BsmBI CGTCTC 2 cut(s) 191, 327
BsmFI GGGAC 2 cut(s) 112, 286
BsnI GGCC 3 cut(s) 18, 24, 262
BsoBI CYCGRG 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 5
BspACI CCGC 8 cut(s) 12, 15, 63, 75, 78, 224, 242, 254
BspANI GGCC 3 cut(s) 18, 24, 262
BspCNI CTCAG 2 cut(s) 115, 156
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 94, 147
BspMI ACCTGC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 24, 226
BssAI RCCGGY 2 cut(s) 24, 226
BssECI CCNNGG 1 cut(s) 19
BssNI GRCGYC 2 cut(s) 93, 230
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 277
BstACI GRCGYC 2 cut(s) 93, 230
BstC8I GCNNGC 2 cut(s) 26, 228
BstDEI CTNAG 2 cut(s) 102, 164
BstENI CCTNNNNNAGG 1 cut(s) 317
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 2 cut(s) 42, 96
BstHHI GCGC 2 cut(s) 41, 95
BstMAI GTCTC 3 cut(s) 111, 191, 327
BstMCI CGRYCG 2 cut(s) 19, 279
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 95, 312
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 140
BstZI CGGCCG 1 cut(s) 16
BsuRI GGCC 3 cut(s) 18, 24, 262
BtgZI GCGATG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 211
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 26, 228
CfoI GCGC 2 cut(s) 41, 95
Cfr10I RCCGGY 2 cut(s) 24, 226
CseI GACGC 2 cut(s) 18, 219
DdeI CTNAG 2 cut(s) 102, 164
DinI GGCGCC 1 cut(s) 94
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 16
EagI CGGCCG 1 cut(s) 16
EciI GGCGGA 2 cut(s) 78, 93
EclXI CGGCCG 1 cut(s) 16
Eco52I CGGCCG 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
Esp3I CGTCTC 2 cut(s) 191, 327
FaqI GGGAC 2 cut(s) 112, 286
Fnu4HI GCNGC 5 cut(s) 13, 16, 76, 129, 224
Fsp4HI GCNGC 5 cut(s) 13, 16, 76, 129, 224
GlaI GCGC 2 cut(s) 40, 94
GluI GCNGC 5 cut(s) 13, 16, 76, 129, 224
HaeII RGCGCY 2 cut(s) 42, 96
HaeIII GGCC 3 cut(s) 18, 24, 262
HapII CCGG 6 cut(s) 6, 25, 36, 96, 152, 227
HgaI GACGC 2 cut(s) 18, 219
HhaI GCGC 2 cut(s) 41, 95
Hin1I GRCGYC 2 cut(s) 93, 230
Hin6I GCGC 2 cut(s) 39, 93
HinP1I GCGC 2 cut(s) 39, 93
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 1 cut(s) 100
HpaII CCGG 6 cut(s) 6, 25, 36, 96, 152, 227
HphI GGTGA 2 cut(s) 256, 332
Hpy166II GTNNAC 2 cut(s) 189, 340
Hpy188I TCNGA 1 cut(s) 299
Hpy188III TCNNGA 3 cut(s) 6, 196, 271
Hpy8I GTNNAC 2 cut(s) 189, 340
Hpy99I CGWCG 4 cut(s) 205, 208, 235, 238
HpyAV CCTTC 1 cut(s) 232
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 2 cut(s) 210, 336
HpyCH4V TGCA 2 cut(s) 319, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 2 cut(s) 102, 164
HpySE526I ACGT 2 cut(s) 210, 336
Hsp92I GRCGYC 2 cut(s) 93, 230
HspAI GCGC 2 cut(s) 39, 93
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 5
KroI GCCGGC 2 cut(s) 24, 226
KroNI GCCGGC 2 cut(s) 26, 228
LmnI GCTCC 2 cut(s) 151, 178
Lsp1109I GCAGC 1 cut(s) 140
MaeII ACGT 2 cut(s) 210, 336
MluCI AATT 2 cut(s) 265, 288
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 7 cut(s) 14, 59, 65, 74, 142, 159, 252
MreI CGCCGGCG 1 cut(s) 226
MroI TCCGGA 1 cut(s) 5
MroNI GCCGGC 2 cut(s) 24, 226
MseI TTAA 1 cut(s) 367
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 6 cut(s) 6, 25, 36, 96, 152, 227
MspR9I CCNGG 2 cut(s) 97, 314
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 21
NaeI GCCGGC 2 cut(s) 26, 228
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 97
NgoMIV GCCGGC 2 cut(s) 24, 226
NlaIV GGNNCC 2 cut(s) 94, 147
NmeAIII GCCGAG 1 cut(s) 44
PaeR7I CTCGAG 1 cut(s) 194
PcsI WCGNNNNNNNCGW 2 cut(s) 16, 216
PdiI GCCGGC 2 cut(s) 26, 228
PflFI GACNNNGTC 1 cut(s) 103
PkrI GCNGC 5 cut(s) 14, 17, 77, 130, 225
PleI GAGTC 1 cut(s) 94
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 94, 147
PsyI GACNNNGTC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 1 cut(s) 367
SatI GCNGC 5 cut(s) 13, 16, 76, 129, 224
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 2 cut(s) 97, 314
SetI ASST 5 cut(s) 70, 213, 251, 324, 339
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 194
SgrAI CRCCGGYG 1 cut(s) 226
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 2 cut(s) 265, 288
SsiI CCGC 8 cut(s) 12, 15, 63, 75, 78, 224, 242, 254
SspDI GGCGCC 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 95, 312
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 2 cut(s) 213, 339
TaqI TCGA 2 cut(s) 195, 236
TasI AATT 2 cut(s) 265, 288
TauI GCSGC 4 cut(s) 15, 18, 78, 226
Tru1I TTAA 1 cut(s) 367
Tru9I TTAA 1 cut(s) 367
TseI GCWGC 1 cut(s) 128
TspGWI ACGGA 1 cut(s) 221
Tth111I GACNNNGTC 1 cut(s) 103
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 2 cut(s) 265, 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.