Rh5BG441300

XH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019827
Physical Location & Seq
Forward (+)
70949991 .. 70953296
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG441300.1

Sequence Viewer

Length: 1914 bp
ATGTCTCACAGATCAGAAGAAATGGATATCGGAAAACTGCAACAAAATGCTCGTGACCATCTTGAAAAGGCCTTCTTGGAGCAAGGCAAAGCCTTTTTAAAATCTGAAGGGCAAAAGAATGATCTTGAGAAAAAGCTTGAACAGAATGAGGCTCAAAAGAAAGATCTGCAGAAAAAGCTTGAGCAGAGTGAACTTACCAAAAAAGAGCTTCAGAAGAAGATTGCAGGTGAACAGGAGTGTCAAGGGTTGTATCTTAAGATTAAGAAAAAGCACGAGCAGAGTGAAACTCAGATGAAAGAGATTCAGAAAAAGCTTGAGCAGAGTGAATCCCACAAGAGAGAGCTTGAGAAGATTGAAGAAGCTCAAAAGAACGAACTTAAGAAGGCGCTTGAGCAGATTGAAGCTCAAAAGAAAGCTATTGAGCAGCGTGAACTTGCCAAAAAAGAGCTTCAGAAGAAGATTGAAGGTGAACAGGTGTGGCTACGGTTGTATCTTGAGGTTAAGAAAAAGAACGAGCAGACTGAAAGTCAGATGAAAGAGATTCAGAAAAAGCTTGAGCACAGTGAATCCCAGAAAATAGAGCTTGAGAAGATTGAAGAAGCTCAAAAGAGCGAACTTAAGAAGGCGCTTGAGCAGATTGAAGGTCAAAAGAAAGATCTTGAGCGGCGTGAACTTGCCAAAAAAGAGCTTCAGAAGAAGATTGAAGGTGAACAGGTGTGGCTACGGTTGTATCTTGAGGTTAAGAAAAAGCACGAGCAGACTGAAAGTCAGATGAAAGAGATTCAGAAAAAGCTTGAGCAGAGTGAATCCCAGAAAAGAGAGCTTGAGAAGATTGAAGAAGCTCAAAAGAGCGAACTTAAGAAGGCGCTTGAGCAGATTGAAGGTCAAAAGAAAGATCTTGAGCAGCGTGAACTTGCCAAAAAAGAGCTTCAGAAGAAGATTGCAGGTGAACAGGCATGTCTAGAGTTGTATCTTAAGGTTAAGAAAAAGCACGAGCAGAGTGAAACTCAGATGAAAGAGATTCAGAAAAAGCTTGAGCAGAGTGAATCCCAGAAGAGAGAGCTTGAGAAGATTGAAGAAGCTCAAAAGAACAAACTTGAGAAGGCGCTTGAGCAGATTGAAGCTCAAAAGAAAGATTTTGAGCAGCGCGAGATGAAATTAAAACAAATGCAGGAGAGGGTTAATATTGAGCAGAGGAAGGCTAATGAAAATTTGTTAAAAGAAAAGAAAGAAAATGAGGAGCTCCGGAACAAGATCACTGAGTTGGAGGATCGTAAGAATCAAGTTCAGGATGAGGAGGATTTAGATGATGAGTCTGAGGACGAGCATGAAGAGATGGATGAAATTCGAGAAAAGTTAAAGGAGAAAGAAGAAGAGCTGGCCAATGTTGAAGCTCTAAACAACACACTTATTGTTAAGGAGCTTAAAAGCAACACTGAACTGCAGGAGGCTCGTAGAGAGATAATCAGAGGATGGGAATCAGCAAGTCGTGCTTTTATTGGTGTCAAGAGAATGGGAGAGCTTGACAGCAAACCATTTCAGACTGCATGCAAGAGAAAATATAATACAGAAGAAGCAGATGACCAGGCAGCAGCACTATGTTCTCTATGGGAGAATTATATTAAGGATTCAAGCTGGCATCCATTCAAAACTAGCAAGGATTCCTTTGGACATTGTAAGGAGATTATTGATGAAGAAGATGAAAAATTGAAAAAGTTAAAGAACGAGTTTGGTGCTGAAGTTCATCTGGCTGTGTCAACTGCTTTGCTGGAATTGAATGAGTATAATCCCAGTGGTAGGTATGCACTTAAAGAACTATGGAACTTTAATCAAGATAGAAGGGCATCATTAAAAGAGGGAATATTGCATCTCCTGAAGCAGTTGAAACTGCAAAAGAGGAGAAGAACCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

637

Amino Acids

75.78

Weight (kDa)

6.46

Isoelectric Point (pI)

63.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XH PF03469 503 - 634 1.8e-53 XH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019827)

Species Orthologous Gene IDs
pyrus_communis pycom11g08770
rosa_multiflora Rmu_sc0002915.1_g000017
rosa_samantha Rh5BG441200 Rh5BG441300 Rh5CG463800 Rh5CG463900
rosa_wichuraiana Rw5G040020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 215, 935
Acc36I ACCTGC 2 cut(s) 215, 935
AccBSI CCGCTC 1 cut(s) 664
AccII CGCG 1 cut(s) 1149
AccIII TCCGGA 1 cut(s) 1245
AciI CCGC 1 cut(s) 664
AclWI GGATC 1 cut(s) 1278
AcoI YGGCCR 1 cut(s) 1380
AcsI RAATTY 2 cut(s) 1210, 1344
AcuI CTGAAG 7 cut(s) 126, 194, 434, 674, 914, 1758, 1895
AfiI CCNNNNNNNGG 1 cut(s) 1797
AflII CTTAAG 5 cut(s) 254, 377, 617, 857, 974
AhdI GACNNNNNGTC 2 cut(s) 525, 765
AjnI CCWGG 1 cut(s) 1584
Alw21I GWGCWC 2 cut(s) 561, 1245
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 1278
Aor13HI TCCGGA 1 cut(s) 1245
AoxI GGCC 2 cut(s) 69, 1380
ApeKI GCWGC 5 cut(s) 424, 904, 1144, 1589, 1592
ApoI RAATTY 2 cut(s) 1210, 1344
AspLEI GCGC 5 cut(s) 388, 628, 868, 1108, 1149
AsuHPI GGTGA 4 cut(s) 239, 479, 719, 959
BaeI ACNNNNGTAYC 4 cut(s) 473, 506, 713, 746
BalI TGGCCA 1 cut(s) 1382
BanII GRGCYC 1 cut(s) 1245
BauI CACGAG 4 cut(s) 51, 272, 752, 992
Bbv12I GWGCWC 2 cut(s) 561, 1245
BbvI GCAGC 5 cut(s) 436, 916, 1156, 1601, 1604
BccI CCATC 3 cut(s) 66, 1330, 1467
BcgI CGANNNNNNTGC 4 cut(s) 1433, 1467, 1715, 1749
BciT130I CCWGG 1 cut(s) 1586
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 962, 1653
BfmI CTRYAG 2 cut(s) 167, 1442
BfoI RGCGCY 4 cut(s) 389, 629, 869, 1109
BfrI CTTAAG 5 cut(s) 254, 377, 617, 857, 974
BfuAI ACCTGC 2 cut(s) 215, 935
BglII AGATCT 3 cut(s) 163, 655, 895
BisI GCNGC 6 cut(s) 425, 665, 905, 1145, 1590, 1593
BlsI GCNGC 6 cut(s) 426, 666, 906, 1146, 1591, 1594
Bme1390I CCNGG 1 cut(s) 1586
BmeRI GACNNNNNGTC 2 cut(s) 525, 765
BmrFI CCNGG 1 cut(s) 1586
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 3 cut(s) 1648, 1853, 1876
BmuI ACTGGG 1 cut(s) 1785
BplI GAGNNNNNCTC 4 cut(s) 271, 303, 991, 1023
BsaBI GATNNNNATC 1 cut(s) 1477
BsaWI WCCGGW 1 cut(s) 1245
Bsc4I CCNNNNNNNGG 1 cut(s) 1797
Bse1I ACTGG 1 cut(s) 1791
Bse8I GATNNNNATC 1 cut(s) 1477
BseAI TCCGGA 1 cut(s) 1245
BseBI CCWGG 1 cut(s) 1586
BseGI GGATG 4 cut(s) 1297, 1345, 1478, 1639
BseJI GATNNNNATC 1 cut(s) 1477
BseLI CCNNNNNNNGG 1 cut(s) 1797
BseMII CTCAG 4 cut(s) 302, 1022, 1251, 1308
BseNI ACTGG 1 cut(s) 1791
BseRI GAGGAG 3 cut(s) 1253, 1310, 1912
BseXI GCAGC 5 cut(s) 436, 916, 1156, 1601, 1604
Bsh1236I CGCG 1 cut(s) 1149
BshFI GGCC 2 cut(s) 71, 1382
BsiHKAI GWGCWC 2 cut(s) 561, 1245
BsiSI CCGG 1 cut(s) 1246
BslI CCNNNNNNNGG 1 cut(s) 1797
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 2 cut(s) 71, 1382
Bsp1286I GDGCHC 2 cut(s) 561, 1245
Bsp13I TCCGGA 1 cut(s) 1245
Bsp143I GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
BspACI CCGC 1 cut(s) 664
BspANI GGCC 2 cut(s) 71, 1382
BspCNI CTCAG 4 cut(s) 301, 1021, 1252, 1309
BspEI TCCGGA 1 cut(s) 1245
BspFNI CGCG 1 cut(s) 1149
BspHI TCATGA 1 cut(s) 1910
BspMAI CTGCAG 2 cut(s) 171, 1446
BspMI ACCTGC 2 cut(s) 215, 935
BspPI GGATC 1 cut(s) 1278
BspQI GCTCTTC 1 cut(s) 1368
BspTI CTTAAG 5 cut(s) 254, 377, 617, 857, 974
BsrBI CCGCTC 1 cut(s) 664
BsrI ACTGG 1 cut(s) 1791
BssMI GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
BssSI CACGAG 4 cut(s) 51, 272, 752, 992
Bst2BI CACGAG 4 cut(s) 51, 272, 752, 992
Bst2UI CCWGG 1 cut(s) 1586
Bst4CI ACNGT 3 cut(s) 486, 563, 726
Bst6I CTCTTC 3 cut(s) 1049, 1326, 1368
BstAFI CTTAAG 5 cut(s) 254, 377, 617, 857, 974
BstAPI GCANNNNNTGC 1 cut(s) 1490
BstC8I GCNNGC 3 cut(s) 1380, 1549, 1637
BstDEI CTNAG 4 cut(s) 288, 1008, 1260, 1317
BstF5I GGATG 4 cut(s) 1297, 1345, 1478, 1639
BstFNI CGCG 1 cut(s) 1149
BstH2I RGCGCY 4 cut(s) 389, 629, 869, 1109
BstHHI GCGC 5 cut(s) 388, 628, 868, 1108, 1149
BstKTI GATC 7 cut(s) 14, 124, 166, 658, 898, 1257, 1273
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
BstMWI GCNNNNNNNGC 2 cut(s) 175, 1490
BstNI CCWGG 1 cut(s) 1586
BstNSI RCATGY 2 cut(s) 960, 1551
BstSCI CCNGG 1 cut(s) 1584
BstSFI CTRYAG 2 cut(s) 167, 1442
BstUI CGCG 1 cut(s) 1149
BstV1I GCAGC 5 cut(s) 436, 916, 1156, 1601, 1604
BstX2I RGATCY 3 cut(s) 163, 655, 895
BstYI RGATCY 3 cut(s) 163, 655, 895
BsuRI GGCC 2 cut(s) 71, 1382
BtsCI GGATG 4 cut(s) 1297, 1345, 1478, 1639
BtsIMutI CAGTG 4 cut(s) 568, 1257, 1434, 1798
BveI ACCTGC 2 cut(s) 215, 935
Cac8I GCNNGC 3 cut(s) 1380, 1549, 1637
CciI TCATGA 1 cut(s) 1910
CfoI GCGC 5 cut(s) 388, 628, 868, 1108, 1149
CviAII CATG 4 cut(s) 957, 1328, 1548, 1911
DdeI CTNAG 4 cut(s) 288, 1008, 1260, 1317
DpnI GATC 7 cut(s) 13, 123, 165, 657, 897, 1256, 1272
DpnII GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
DraI TTTAAA 1 cut(s) 99
DriI GACNNNNNGTC 2 cut(s) 525, 765
EaeI YGGCCR 1 cut(s) 1380
Eam1104I CTCTTC 3 cut(s) 1049, 1326, 1368
Eam1105I GACNNNNNGTC 2 cut(s) 525, 765
EarI CTCTTC 3 cut(s) 1049, 1326, 1368
Ecl136II GAGCTC 1 cut(s) 1243
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 1245
Eco32I GATATC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 1243
Eco57I CTGAAG 7 cut(s) 126, 194, 434, 674, 914, 1758, 1895
EcoICRI GAGCTC 1 cut(s) 1243
EcoRII CCWGG 1 cut(s) 1584
EcoRV GATATC 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 1245
FaeI CATG 4 cut(s) 960, 1331, 1551, 1914
FalI AAGNNNNNCTT 6 cut(s) 59, 91, 1477, 1509, 1649, 1681
FatI CATG 4 cut(s) 956, 1327, 1547, 1910
Fnu4HI GCNGC 6 cut(s) 425, 665, 905, 1145, 1590, 1593
FokI GGATG 4 cut(s) 1304, 1352, 1485, 1626
FriOI GRGCYC 1 cut(s) 1245
Fsp4HI GCNGC 6 cut(s) 425, 665, 905, 1145, 1590, 1593
FspBI CTAG 2 cut(s) 962, 1653
GlaI GCGC 5 cut(s) 387, 627, 867, 1107, 1148
GluI GCNGC 6 cut(s) 425, 665, 905, 1145, 1590, 1593
HaeII RGCGCY 4 cut(s) 389, 629, 869, 1109
HaeIII GGCC 2 cut(s) 71, 1382
HapII CCGG 1 cut(s) 1246
HhaI GCGC 5 cut(s) 388, 628, 868, 1108, 1149
Hin1II CATG 4 cut(s) 960, 1331, 1551, 1914
Hin6I GCGC 5 cut(s) 386, 626, 866, 1106, 1147
HinP1I GCGC 5 cut(s) 386, 626, 866, 1106, 1147
HincII GTYRAC 1 cut(s) 1758
HindII GTYRAC 1 cut(s) 1758
HindIII AAGCTT 6 cut(s) 134, 176, 311, 551, 791, 1031
HpaII CCGG 1 cut(s) 1246
HphI GGTGA 4 cut(s) 239, 479, 719, 959
Hpy166II GTNNAC 9 cut(s) 191, 230, 431, 470, 671, 710, 911, 950, 1758
Hpy8I GTNNAC 9 cut(s) 191, 230, 431, 470, 671, 710, 911, 950, 1758
HpyCH4III ACNGT 3 cut(s) 486, 563, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 1490
HpyF3I CTNAG 4 cut(s) 288, 1008, 1260, 1317
Hsp92II CATG 4 cut(s) 960, 1331, 1551, 1914
HspAI GCGC 5 cut(s) 386, 626, 866, 1106, 1147
Kpn2I TCCGGA 1 cut(s) 1245
Kzo9I GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
LguI GCTCTTC 1 cut(s) 1368
LmnI GCTCC 4 cut(s) 79, 1240, 1248, 1420
Lsp1109I GCAGC 5 cut(s) 436, 916, 1156, 1601, 1604
LweI GCATC 3 cut(s) 1648, 1853, 1876
MaeI CTAG 2 cut(s) 962, 1653
MaeIII GTNAC 1 cut(s) 53
MalI GATC 7 cut(s) 13, 123, 165, 657, 897, 1256, 1272
MbiI CCGCTC 1 cut(s) 664
MboI GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
MflI RGATCY 3 cut(s) 163, 655, 895
MhlI GDGCHC 2 cut(s) 561, 1245
MlsI TGGCCA 1 cut(s) 1382
MluCI AATT 6 cut(s) 1157, 1210, 1344, 1615, 1706, 1772
MluNI TGGCCA 1 cut(s) 1382
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 1 cut(s) 1245
Mox20I TGGCCA 1 cut(s) 1382
MroI TCCGGA 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 1382
Msp20I TGGCCA 1 cut(s) 1382
MspCI CTTAAG 5 cut(s) 254, 377, 617, 857, 974
MspI CCGG 1 cut(s) 1246
MspR9I CCNGG 1 cut(s) 1586
MvaI CCWGG 1 cut(s) 1586
MvnI CGCG 1 cut(s) 1149
MwoI GCNNNNNNNGC 2 cut(s) 175, 1490
NdeII GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
NlaIII CATG 4 cut(s) 960, 1331, 1551, 1914
NmuCI GTSAC 1 cut(s) 53
NspI RCATGY 2 cut(s) 960, 1551
PaeI GCATGC 1 cut(s) 1551
PagI TCATGA 1 cut(s) 1910
PaqCI CACCTGC 2 cut(s) 215, 935
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 1368
PkrI GCNGC 6 cut(s) 426, 666, 906, 1146, 1591, 1594
PleI GAGTC 1 cut(s) 1321
PpsI GAGTC 1 cut(s) 1321
Psp124BI GAGCTC 1 cut(s) 1245
Psp6I CCWGG 1 cut(s) 1584
PspGI CCWGG 1 cut(s) 1584
PstI CTGCAG 2 cut(s) 171, 1446
PsuI RGATCY 3 cut(s) 163, 655, 895
SacI GAGCTC 1 cut(s) 1245
SapI GCTCTTC 1 cut(s) 1368
SatI GCNGC 6 cut(s) 425, 665, 905, 1145, 1590, 1593
Sau3AI GATC 7 cut(s) 11, 121, 163, 655, 895, 1254, 1270
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 1 cut(s) 1586
SduI GDGCHC 2 cut(s) 561, 1245
SfaNI GCATC 3 cut(s) 1648, 1853, 1876
SfcI CTRYAG 2 cut(s) 167, 1442
SphI GCATGC 1 cut(s) 1551
Sse9I AATT 6 cut(s) 1157, 1210, 1344, 1615, 1706, 1772
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 1 cut(s) 664
SspI AATATT 2 cut(s) 1186, 1863
SspMI CTAG 2 cut(s) 962, 1653
SstI GAGCTC 1 cut(s) 1245
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 1584
TaaI ACNGT 3 cut(s) 486, 563, 726
TaqI TCGA 1 cut(s) 1348
TasI AATT 6 cut(s) 1157, 1210, 1344, 1615, 1706, 1772
TauI GCSGC 1 cut(s) 667
TscAI CASTG 4 cut(s) 568, 1264, 1441, 1798
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 5 cut(s) 424, 904, 1144, 1589, 1592
Tsp45I GTSAC 1 cut(s) 53
TspRI CASTG 4 cut(s) 568, 1264, 1441, 1798
Vha464I CTTAAG 5 cut(s) 254, 377, 617, 857, 974
XapI RAATTY 2 cut(s) 1210, 1344
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 2 cut(s) 960, 1551
XspI CTAG 2 cut(s) 962, 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.