Rh5BG494300

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0021275
Physical Location & Seq
Forward (+)
78729363 .. 78732222
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG494300.1

Sequence Viewer

Length: 432 bp
ATGGCTTCATCTACAAGATTTTCTTTCTGTTCTGTAGTGGTCATTGTCACTTTGGGTACTCTTGCTGGTTTCACAGGGAGATCTTCTGCTCAACTCTCAACGAATTTCTATTCGGGAAGCTGCCCTAAGGTTTTCAGCACTGTCAAGTCCGTTGTACAGTCTGCGATCTCCAAGGAAAATCGCATGGGGGCTTCTCTCCTTCGCCTTCACTTCCATGACTGCTTCGTCAATGGTTGTGATGGGTCTGTACTCCTAGACGACACTTCTTCTTTCACTGGTGAGAAGACAGCAGGTCCCAACAATAATTCAATTAGGGGATTTGAAGTCATTGATAAGATAAAAACCCGGGTCGAAAAAGCATGCCCCGGCGTCGTTTCGTGTGCTGATATCCTTACCATTGCCGCTCGAGACTCCGTTGTGATTGTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

143

Amino Acids

15.09

Weight (kDa)

8.6

Isoelectric Point (pI)

25.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 141 1.4e-37 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021275)

Species Orthologous Gene IDs
pyrus_communis pycom11g05050
rosa_multiflora Rmu_co8450017.1_g000001
rosa_samantha Rh4CG453600 Rh5BG494300 Rh5DG505500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 404
AciI CCGC 1 cut(s) 402
AcsI RAATTY 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 3 cut(s) 58, 156, 249
AgsI TTSAA 2 cut(s) 309, 323
AhdI GACNNNNNGTC 1 cut(s) 291
AluBI AGCT 1 cut(s) 120
AluI AGCT 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 402
Ama87I CYCGRG 2 cut(s) 345, 405
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 3 cut(s) 346, 347, 366
AsuHPI GGTGA 1 cut(s) 290
AvaI CYCGRG 2 cut(s) 345, 405
AvaII GGWCC 1 cut(s) 293
AxyI CCTNAGG 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 233
BcnI CCSGG 3 cut(s) 346, 347, 366
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 80
BisI GCNGC 2 cut(s) 121, 402
BlsI GCNGC 2 cut(s) 122, 403
Bme1390I CCNGG 3 cut(s) 346, 347, 366
Bme18I GGWCC 1 cut(s) 293
BmeRI GACNNNNNGTC 1 cut(s) 291
BmeT110I CYCGRG 2 cut(s) 345, 405
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 3 cut(s) 346, 347, 366
BpiI GAAGAC 1 cut(s) 290
BpuMI CCSGG 3 cut(s) 346, 347, 366
BsaHI GRCGYC 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 171, 345, 364
Bse1I ACTGG 1 cut(s) 280
Bse21I CCTNAGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 396
BseDI CCNNGG 3 cut(s) 171, 345, 364
BseMI GCAATG 1 cut(s) 396
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 1 cut(s) 107
BsiHKCI CYCGRG 2 cut(s) 345, 405
BsiSI CCGG 2 cut(s) 346, 366
BslFI GGGAC 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 279
BsoBI CYCGRG 2 cut(s) 345, 405
Bsp1407I TGTACA 1 cut(s) 154
Bsp143I GATC 2 cut(s) 80, 165
BspACI CCGC 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 281
BsrBI CCGCTC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 171, 345, 364
BssMI GATC 2 cut(s) 80, 165
BssNI GRCGYC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 142, 159
BstACI GRCGYC 1 cut(s) 369
BstAUI TGTACA 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 2 cut(s) 83, 168
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 2 cut(s) 80, 165
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 3 cut(s) 344, 345, 364
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
Bsu36I CCTNAGG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 138, 273
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 361
Cfr13I GGNCC 1 cut(s) 293
Cfr9I CCCGGG 1 cut(s) 345
CseI GACGC 1 cut(s) 358
Csp6I GTAC 3 cut(s) 57, 155, 248
CviAII CATG 3 cut(s) 184, 215, 360
CviJI RGCY 3 cut(s) 5, 120, 191
CviKI_1 RGCY 3 cut(s) 5, 120, 191
CviQI GTAC 3 cut(s) 57, 155, 248
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 82, 167
DpnII GATC 2 cut(s) 80, 165
DrdI GACNNNNNNGTC 1 cut(s) 224
DriI GACNNNNNGTC 1 cut(s) 291
DseDI GACNNNNNNGTC 1 cut(s) 224
Eam1105I GACNNNNNGTC 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 171
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 2 cut(s) 345, 405
EcoO109I RGGNCCY 1 cut(s) 293
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 3 cut(s) 187, 218, 363
FaiI YATR 3 cut(s) 185, 216, 361
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 183, 214, 359
Fnu4HI GCNGC 2 cut(s) 121, 402
Fsp4HI GCNGC 2 cut(s) 121, 402
FspBI CTAG 1 cut(s) 254
GluI GCNGC 2 cut(s) 121, 402
HapII CCGG 2 cut(s) 346, 366
HgaI GACGC 1 cut(s) 358
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 3 cut(s) 187, 218, 363
HinfI GANTC 1 cut(s) 410
HpaII CCGG 2 cut(s) 346, 366
HphI GGTGA 1 cut(s) 290
Hpy188III TCNNGA 2 cut(s) 114, 407
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 2 cut(s) 209, 215
HpyCH4III ACNGT 2 cut(s) 142, 159
HpyF3I CTNAG 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 3 cut(s) 187, 218, 363
Kzo9I GATC 2 cut(s) 80, 165
LpnPI CCDG 6 cut(s) 51, 60, 261, 276, 359, 379
Lsp1109I GCAGC 1 cut(s) 107
MaeI CTAG 1 cut(s) 254
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 82, 167
MbiI CCGCTC 1 cut(s) 404
MboI GATC 2 cut(s) 80, 165
MboII GAAGA 3 cut(s) 75, 258, 295
MflI RGATCY 1 cut(s) 80
MluCI AATT 3 cut(s) 103, 304, 309
MlyI GAGTC 1 cut(s) 404
MseI TTAA 1 cut(s) 430
MslI CAYNNNNRTG 1 cut(s) 213
MspI CCGG 2 cut(s) 346, 366
MspR9I CCNGG 3 cut(s) 346, 347, 366
NciI CCSGG 3 cut(s) 346, 347, 366
NdeII GATC 2 cut(s) 80, 165
NlaIII CATG 3 cut(s) 187, 218, 363
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 363
PaeI GCATGC 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 405
PkrI GCNGC 2 cut(s) 122, 403
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 3 cut(s) 58, 156, 249
RsaNI GTAC 3 cut(s) 57, 155, 248
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 1 cut(s) 430
SatI GCNGC 2 cut(s) 121, 402
Sau3AI GATC 2 cut(s) 80, 165
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 346, 347, 366
SetI ASST 3 cut(s) 122, 132, 295
SfcI CTRYAG 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 405
SmaI CCCGGG 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 1 cut(s) 363
Sse9I AATT 3 cut(s) 103, 304, 309
SsiI CCGC 1 cut(s) 402
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 3 cut(s) 344, 345, 364
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 142, 159
TaqI TCGA 2 cut(s) 351, 406
TasI AATT 3 cut(s) 103, 304, 309
TatI WGTACW 2 cut(s) 154, 247
TauI GCSGC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 430
Tru9I TTAA 1 cut(s) 430
TscAI CASTG 2 cut(s) 145, 280
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 46
TspGWI ACGGA 2 cut(s) 139, 403
TspMI CCCGGG 1 cut(s) 345
TspRI CASTG 2 cut(s) 145, 280
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 103
XceI RCATGY 1 cut(s) 363
XhoI CTCGAG 1 cut(s) 405
XmaI CCCGGG 1 cut(s) 345
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.