Rh5CG080900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010071
Physical Location & Seq
Forward (+)
6213234 .. 6214667
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG080900.1

Sequence Viewer

Length: 1272 bp
ATGGGGAGTAGAACCCTGTTTAAATGGTCGAAGCAAATTACTAATTCCCAAGTTGGGCAGCTGATAAAAGCTGAGAAGGACCTCCAGAAAGCGCTTATCATATTTGATTCCGCAACAGCTGAGTACACCAATGGTTTTCGGCATGATCATAGCACGTTTGGGATCATGATCTCTAGATTAGTCTCTGCAAACCAGTTCAGGTCTGCGGAGGCGCTGCTTGATAGGATGAAGGAGGAGAAGTGTAGTGTTACGGAAGATATATTCTTGTCTGTTTGTAGAGGGTATGCTAGAGTTCACAGGCCACTGGATTCTGTCAGGGTGTTCGACAAGATGGAGGACTTCCAGTGCAAACCAACTGAAAAGTCTTACATTACGATATTTGGGATTCTTGTTGAAGAAAACCAGTTAAAGGTTGCTTTCAGATTCTACAGGTATATGAGGAAAACGGGCATTCCAGCTAGTGTTGTTTCACTTAATATCCTGATTAAAGCGCTTTGTAAGAACAGCAGTTCCATGGATGCAGCTCTTCGGATACTTCGTGAGATGCCTGATCATGGGTGCACCCCTGATTCATATACATATGGTACTTTGATTAATGGATTGTGCAAGTTAGGAAAGATTGGTAAAGCAAAGGAGCTATTCTGTGAGATGGGAACAAAAGGTTGTTCACCATCTGTTGTTACGTATACTTGCTTGATGCGTGGTTTGTGCCAATCTAACAATTTGGATGAAGCTGTGGAATTGTTTGAAGACATGAAAGACAAAGGGAAGCTTCGAGAGGCTTTGGAGATTCTGGACAAAATGAAGCTGCAGGGATTGAAACCAGATGCAGGGCAGTATGGGAAAATAATAACTGGCTTCTGCAATGTTTCCAAGTTTCAAGAAGCAGCAAACTTTCTTGATGAGATGTTCCTTGGTGGGATTTCACCCAACAGATTAACCTGGAGCCTTCATGTTAGGATCCATAATGCAGTGGTTCAAGGCTTGTGCAGTAGTGGCAACCTAACTCGAGCTTTTCAGTTGTATCTTAGCATGCGCACCAGGGGTTTCTCAATTGATTTGCAAACTTTTGATACTCTAGTGAAATGCATTTCTAAGAAAGGTGACCTGCACAAAGCTTATCGAATTGTTGATGAGATGGTGCTTGATGGATGCATTCCAGACTATGGAATTTGGAATGCTGTGGTCAAAGGGTTTTGGGATCGCACAAAGGTGCGTGAACCTGCCGAGTTGTTGCAGGCTGAGCTGATGAGCGAATTAGCTGATGAATAA

Protein Analysis

423

Amino Acids

47.75

Weight (kDa)

8.53

Isoelectric Point (pI)

27.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 104 - 185 4.9e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 122 - 167 6.6e-09 PPR repeat family
PPR_long PF17177 134 - 254 2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 154 - 203 3.1e-16 PPR repeat family
PPR_3 PF13812 177 - 233 4.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 186 - 217 7e-13 PPR repeat
PPR PF01535 192 - 222 1.5e-08 PPR repeat
PPR_2 PF13041 196 - 238 5.4e-15 PPR repeat family
PPR_1 PF12854 220 - 253 6.8e-14 PPR repeat
PPR_2 PF13041 224 - 256 1.5e-10 PPR repeat family
PPR PF01535 227 - 256 2.3e-09 PPR repeat
PPR_2 PF13041 323 - 364 3.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1037
Acc36I ACCTGC 2 cut(s) 1116, 1231
AccB7I CCANNNNNTGG 1 cut(s) 1166
AccI GTMKAC 1 cut(s) 686
AciI CCGC 2 cut(s) 111, 206
AclWI GGATC 4 cut(s) 170, 955, 968, 1209
AcsI RAATTY 1 cut(s) 1170
AfaI GTAC 2 cut(s) 125, 586
AfeI AGCGCT 2 cut(s) 93, 492
AfiI CCNNNNNNNGG 5 cut(s) 54, 409, 554, 830, 1166
AgsI TTSAA 5 cut(s) 395, 749, 820, 881, 980
AjnI CCWGG 2 cut(s) 941, 1040
AleI CACNNNNGTG 1 cut(s) 1211
AloI GAACNNNNNNTCC 2 cut(s) 494, 526
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 187
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 4 cut(s) 170, 955, 968, 1209
Ama87I CYCGRG 1 cut(s) 1008
Aor51HI AGCGCT 2 cut(s) 93, 492
AoxI GGCC 1 cut(s) 299
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 5 cut(s) 58, 214, 521, 808, 887
ApoI RAATTY 1 cut(s) 1170
AseI ATTAAT 1 cut(s) 594
AspLEI GCGC 4 cut(s) 94, 214, 493, 1038
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 3 cut(s) 660, 918, 1115
AvaI CYCGRG 1 cut(s) 1008
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 563
BaeI ACNNNNGTAYC 2 cut(s) 1065, 1098
BamHI GGATCC 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 563
BbvI GCAGC 5 cut(s) 70, 201, 533, 795, 899
BccI CCATC 5 cut(s) 325, 643, 679, 1132, 1142
BciT130I CCWGG 2 cut(s) 943, 1042
BciVI GTATCC 1 cut(s) 525
BclI TGATCA 2 cut(s) 145, 550
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 4 cut(s) 174, 288, 459, 1079
BfmI CTRYAG 2 cut(s) 427, 809
BfoI RGCGCY 3 cut(s) 95, 215, 494
BfuAI ACCTGC 2 cut(s) 1116, 1231
BfuI GTATCC 1 cut(s) 525
BisI GCNGC 5 cut(s) 59, 215, 522, 809, 888
BlpI GCTNAGC 1 cut(s) 1242
BlsI GCNGC 5 cut(s) 60, 216, 523, 810, 889
Bme1390I CCNGG 2 cut(s) 943, 1042
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 1008
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 947, 962
BmrFI CCNGG 2 cut(s) 943, 1042
BmsI GCATC 5 cut(s) 508, 534, 687, 817, 1142
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 2 cut(s) 68, 964
Bpu1102I GCTNAGC 1 cut(s) 1242
BsaAI YACGTR 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 513, 913, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 409, 554, 830, 1166
Bse1I ACTGG 5 cut(s) 193, 309, 343, 403, 859
Bse3DI GCAATG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 2 cut(s) 943, 1042
BseDI CCNNGG 3 cut(s) 513, 913, 1041
BseGI GGATG 4 cut(s) 231, 523, 733, 1157
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 5 cut(s) 54, 409, 554, 830, 1166
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 3 cut(s) 63, 111, 1233
BseNI ACTGG 5 cut(s) 193, 309, 343, 403, 859
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 5 cut(s) 70, 201, 533, 795, 899
BsgI GTGCAG 2 cut(s) 1009, 1094
BshFI GGCC 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 1008
BslI CCNNNNNNNGG 5 cut(s) 54, 409, 554, 830, 1166
BsmAI GTCTC 1 cut(s) 187
BsmI GAATGC 3 cut(s) 450, 1155, 1183
BsnI GGCC 1 cut(s) 301
BsoBI CYCGRG 1 cut(s) 1008
Bsp1286I GDGCHC 1 cut(s) 563
Bsp143I GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
Bsp1720I GCTNAGC 1 cut(s) 1242
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 2 cut(s) 111, 206
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 3 cut(s) 64, 112, 1234
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 947, 962
BspMAI CTGCAG 1 cut(s) 813
BspMI ACCTGC 2 cut(s) 1116, 1231
BspPI GGATC 4 cut(s) 170, 955, 968, 1209
BspQI GCTCTTC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 871
BsrI ACTGG 5 cut(s) 193, 309, 343, 403, 859
BssECI CCNNGG 3 cut(s) 513, 913, 1041
BssMI GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
BssNAI GTATAC 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 513, 913
Bst1107I GTATAC 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 943, 1042
Bst6I CTCTTC 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 1034, 1239
BstDEI CTNAG 5 cut(s) 72, 120, 1028, 1095, 1242
BstDSI CCRYGG 1 cut(s) 513
BstEII GGTNACC 1 cut(s) 1103
BstF5I GGATG 4 cut(s) 231, 523, 733, 1157
BstH2I RGCGCY 3 cut(s) 95, 215, 494
BstHHI GCGC 4 cut(s) 94, 214, 493, 1038
BstKTI GATC 6 cut(s) 148, 165, 171, 553, 963, 1204
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
BstMWI GCNNNNNNNGC 2 cut(s) 996, 1243
BstNI CCWGG 2 cut(s) 943, 1042
BstNSI RCATGY 1 cut(s) 1036
BstPI GGTNACC 1 cut(s) 1103
BstSCI CCNGG 2 cut(s) 941, 1040
BstSFI CTRYAG 2 cut(s) 427, 809
BstSLI GKGCMC 1 cut(s) 563
BstSNI TACGTA 1 cut(s) 684
BstV1I GCAGC 5 cut(s) 70, 201, 533, 795, 899
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 1 cut(s) 960
BstYI RGATCY 1 cut(s) 960
BstZ17I GTATAC 1 cut(s) 687
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 4 cut(s) 231, 523, 733, 1157
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 3 cut(s) 302, 350, 978
BveI ACCTGC 2 cut(s) 1116, 1231
Cac8I GCNNGC 2 cut(s) 1034, 1239
CciI TCATGA 1 cut(s) 165
CfoI GCGC 4 cut(s) 94, 214, 493, 1038
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 124, 585
CviAII CATG 7 cut(s) 143, 166, 514, 554, 754, 953, 1033
CviQI GTAC 2 cut(s) 124, 585
DdeI CTNAG 5 cut(s) 72, 120, 1028, 1095, 1242
DpnI GATC 6 cut(s) 147, 164, 170, 552, 962, 1203
DpnII GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
DraI TTTAAA 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco105I TACGTA 1 cut(s) 684
Eco130I CCWWGG 2 cut(s) 513, 913
Eco47I GGWCC 1 cut(s) 79
Eco47III AGCGCT 2 cut(s) 93, 492
Eco88I CYCGRG 1 cut(s) 1008
Eco91I GGTNACC 1 cut(s) 1103
EcoO109I RGGNCCY 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 1103
EcoRII CCWGG 2 cut(s) 941, 1040
EcoT14I CCWWGG 2 cut(s) 513, 913
EcoT22I ATGCAT 2 cut(s) 1091, 1157
ErhI CCWWGG 2 cut(s) 513, 913
FaeI CATG 7 cut(s) 146, 169, 517, 557, 757, 956, 1036
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FatI CATG 7 cut(s) 142, 165, 513, 553, 753, 952, 1032
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 2 cut(s) 145, 550
FblI GTMKAC 1 cut(s) 686
Fnu4HI GCNGC 5 cut(s) 59, 215, 522, 809, 888
FokI GGATG 4 cut(s) 238, 530, 740, 1164
Fsp4HI GCNGC 5 cut(s) 59, 215, 522, 809, 888
FspAI RTGCGCAY 1 cut(s) 1037
FspBI CTAG 4 cut(s) 174, 288, 459, 1079
FspI TGCGCA 1 cut(s) 1037
GlaI GCGC 4 cut(s) 93, 213, 492, 1037
GluI GCNGC 5 cut(s) 59, 215, 522, 809, 888
GsuI CTGGAG 2 cut(s) 68, 964
HaeII RGCGCY 3 cut(s) 95, 215, 494
HaeIII GGCC 1 cut(s) 301
HhaI GCGC 4 cut(s) 94, 214, 493, 1038
Hin1II CATG 7 cut(s) 146, 169, 517, 557, 757, 956, 1036
Hin6I GCGC 4 cut(s) 92, 212, 491, 1036
HinP1I GCGC 4 cut(s) 92, 212, 491, 1036
HindIII AAGCTT 2 cut(s) 770, 1116
HinfI GANTC 6 cut(s) 107, 308, 385, 423, 569, 790
HphI GGTGA 3 cut(s) 660, 918, 1115
Hpy166II GTNNAC 6 cut(s) 126, 295, 561, 668, 687, 1220
Hpy188I TCNGA 2 cut(s) 422, 531
Hpy8I GTNNAC 6 cut(s) 126, 295, 561, 668, 687, 1220
HpyAV CCTTC 3 cut(s) 70, 223, 959
HpyCH4IV ACGT 2 cut(s) 155, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 996, 1243
HpyF3I CTNAG 5 cut(s) 72, 120, 1028, 1095, 1242
HpySE526I ACGT 2 cut(s) 155, 683
Hsp92II CATG 7 cut(s) 146, 169, 517, 557, 757, 956, 1036
HspAI GCGC 4 cut(s) 92, 212, 491, 1036
Ksp22I TGATCA 2 cut(s) 145, 550
Kzo9I GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 2 cut(s) 634, 945
Lsp1109I GCAGC 5 cut(s) 70, 201, 533, 795, 899
LweI GCATC 5 cut(s) 508, 534, 687, 817, 1142
MaeI CTAG 4 cut(s) 174, 288, 459, 1079
MaeII ACGT 2 cut(s) 155, 683
MaeIII GTNAC 3 cut(s) 247, 679, 1103
MalI GATC 6 cut(s) 147, 164, 170, 552, 962, 1203
MboI GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
MboII GAAGA 4 cut(s) 266, 407, 518, 761
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 1 cut(s) 960
MhlI GDGCHC 1 cut(s) 563
MluCI AATT 8 cut(s) 36, 43, 721, 740, 1053, 1125, 1170, 1256
MnlI CCTC 7 cut(s) 92, 202, 226, 272, 328, 432, 772
Mph1103I ATGCAT 2 cut(s) 1091, 1157
MseI TTAA 6 cut(s) 21, 407, 474, 486, 594, 938
MslI CAYNNNNRTG 1 cut(s) 1211
MspA1I CMGCKG 2 cut(s) 61, 119
MspR9I CCNGG 2 cut(s) 943, 1042
MunI CAATTG 1 cut(s) 1053
Mva1269I GAATGC 3 cut(s) 450, 1155, 1183
MvaI CCWGG 2 cut(s) 943, 1042
MwoI GCNNNNNNNGC 2 cut(s) 996, 1243
NcoI CCATGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 580
NdeII GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
NlaIII CATG 7 cut(s) 146, 169, 517, 557, 757, 956, 1036
NlaIV GGNNCC 2 cut(s) 947, 962
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 1 cut(s) 1103
NsbI TGCGCA 1 cut(s) 1037
NsiI ATGCAT 2 cut(s) 1091, 1157
NspI RCATGY 1 cut(s) 1036
OliI CACNNNNGTG 1 cut(s) 1211
PaeI GCATGC 1 cut(s) 1036
PaeR7I CTCGAG 1 cut(s) 1008
PagI TCATGA 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PctI GAATGC 3 cut(s) 450, 1155, 1183
PfeI GAWTC 6 cut(s) 107, 308, 385, 423, 569, 790
PflMI CCANNNNNTGG 1 cut(s) 1166
PkrI GCNGC 5 cut(s) 60, 216, 523, 810, 889
Ppu21I YACGTR 1 cut(s) 684
PpuMI RGGWCCY 1 cut(s) 79
PshBI ATTAAT 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 941, 1040
PspEI GGTNACC 1 cut(s) 1103
PspGI CCWGG 2 cut(s) 941, 1040
PspN4I GGNNCC 2 cut(s) 947, 962
PspPI GGNCC 1 cut(s) 79
PspPPI RGGWCCY 1 cut(s) 79
PspXI VCTCGAGB 1 cut(s) 1008
PstI CTGCAG 1 cut(s) 813
PsuI RGATCY 1 cut(s) 960
PvuII CAGCTG 2 cut(s) 61, 119
RsaI GTAC 2 cut(s) 125, 586
RsaNI GTAC 2 cut(s) 124, 585
RseI CAYNNNNRTG 1 cut(s) 1211
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 6 cut(s) 21, 407, 474, 486, 594, 938
SatI GCNGC 5 cut(s) 59, 215, 522, 809, 888
Sau3AI GATC 6 cut(s) 145, 162, 168, 550, 960, 1201
Sau96I GGNCC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 943, 1042
SduI GDGCHC 1 cut(s) 563
SfaNI GCATC 5 cut(s) 508, 534, 687, 817, 1142
SfcI CTRYAG 2 cut(s) 427, 809
Sfr274I CTCGAG 1 cut(s) 1008
SinI GGWCC 1 cut(s) 79
SlaI CTCGAG 1 cut(s) 1008
SmiMI CAYNNNNRTG 1 cut(s) 1211
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
SnaBI TACGTA 1 cut(s) 684
SphI GCATGC 1 cut(s) 1036
Sse9I AATT 8 cut(s) 36, 43, 721, 740, 1053, 1125, 1170, 1256
SsiI CCGC 2 cut(s) 111, 206
SspMI CTAG 4 cut(s) 174, 288, 459, 1079
StyD4I CCNGG 2 cut(s) 941, 1040
StyI CCWWGG 2 cut(s) 513, 913
TaiI ACGT 2 cut(s) 158, 686
TaqI TCGA 5 cut(s) 29, 324, 775, 1009, 1123
TasI AATT 8 cut(s) 36, 43, 721, 740, 1053, 1125, 1170, 1256
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 6 cut(s) 107, 308, 385, 423, 569, 790
Tru1I TTAA 6 cut(s) 21, 407, 474, 486, 594, 938
Tru9I TTAA 6 cut(s) 21, 407, 474, 486, 594, 938
TscAI CASTG 3 cut(s) 309, 350, 978
TseFI GTSAC 1 cut(s) 1103
TseI GCWGC 5 cut(s) 58, 214, 521, 808, 887
Tsp45I GTSAC 1 cut(s) 1103
TspDTI ATGAA 6 cut(s) 242, 561, 744, 770, 818, 941
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 3 cut(s) 309, 350, 978
Van91I CCANNNNNTGG 1 cut(s) 1166
VneI GTGCAC 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 79
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 1 cut(s) 1170
XbaI TCTAGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 1036
XhoI CTCGAG 1 cut(s) 1008
XmiI GTMKAC 1 cut(s) 686
XspI CTAG 4 cut(s) 174, 288, 459, 1079
Zsp2I ATGCAT 2 cut(s) 1091, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.