Rh5CG132500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0021419
Physical Location & Seq
Reverse (-)
11091465 .. 11092016
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG132500.1

Sequence Viewer

Length: 393 bp
ATGGGGTCATTGTGCGTAATCGAGCTCCAGCACGTGGCATTCATCATGGTGTTCTCTGCCAGTGGCCTCGTCCCGCTGCTTGATTTGATCTTCCCTGCTTTCGCCTCTGCCTATCTCTTAGTACTGTCGTGCTTGGCCTTCCCATCAAGAGATTTTCTAAGGAAGCAAATTCTTCAGGCTTGTTTTCGCCACCTGTTAGAGCACTCATTTCGTTGCTTTACAGAGTTAGGAGAATCTTTGTCATCCTTGATTGGATATGTGAAGCATGTCGGCTGGCTTAACAAGACTCTCCCTGCCAATGCACAATTGAAGGATGTTGCACGGTTTTTGTCTGGAAGGACCTTGGCTAGCTGTGGCCAACTTCTTGTATTTCTTGAAGGACCTTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.36

Weight (kDa)

8.36

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 8 - 63 1.4e-12 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021419)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015761 RchiOBHm_Chr5g0015841 RchiOBHm_Chr5g0015851
rosa_samantha Rh5CG132500
rosa_wichuraiana Rw5G010620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 34
AciI CCGC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 158
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 2 cut(s) 310, 377
AluBI AGCT 2 cut(s) 25, 351
AluI AGCT 2 cut(s) 25, 351
Alw21I GWGCWC 2 cut(s) 27, 204
AoxI GGCC 3 cut(s) 64, 135, 355
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 339, 380
AsuNHI GCTAGC 1 cut(s) 347
AvaII GGWCC 2 cut(s) 339, 380
BalI TGGCCA 1 cut(s) 357
BanII GRGCYC 1 cut(s) 27
BbrPI CACGTG 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 27, 204
BbvI GCAGC 1 cut(s) 63
BccI CCATC 1 cut(s) 151
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BfaI CTAG 1 cut(s) 348
BisI GCNGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 78
BmcAI AGTACT 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 339, 380
BmgT120I GGNCC 2 cut(s) 339, 380
BmtI GCTAGC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 2 cut(s) 242, 319
BseLI CCNNNNNNNGG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 63
BshFI GGCC 3 cut(s) 66, 137, 357
BsiHKAI GWGCWC 2 cut(s) 27, 204
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 3 cut(s) 66, 137, 357
Bsp1286I GDGCHC 2 cut(s) 27, 204
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 1 cut(s) 74
BspANI GGCC 3 cut(s) 66, 137, 357
BspOI GCTAGC 1 cut(s) 351
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 126, 324
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 275, 349
BstDEI CTNAG 2 cut(s) 118, 158
BstF5I GGATG 2 cut(s) 242, 319
BstKTI GATC 1 cut(s) 90
BstMBI GATC 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 63
BsuRI GGCC 3 cut(s) 66, 137, 357
BtsCI GGATG 2 cut(s) 242, 319
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 275, 349
Cfr13I GGNCC 2 cut(s) 339, 380
Csp6I GTAC 1 cut(s) 122
CviAII CATG 2 cut(s) 46, 266
CviJI RGCY 9 cut(s) 25, 66, 137, 179, 273, 277, 347, 351, 357
CviKI_1 RGCY 9 cut(s) 25, 66, 137, 179, 273, 277, 347, 351, 357
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 2 cut(s) 118, 158
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 355
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 339, 380
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 158
Eco72I CACGTG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 339, 380
EcoT14I CCWWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 2 cut(s) 49, 269
FaiI YATR 3 cut(s) 47, 258, 267
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 45, 265
FauI CCCGC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 77
FokI GGATG 2 cut(s) 229, 326
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 1 cut(s) 348
GluI GCNGC 1 cut(s) 77
GsuI CTGGAG 1 cut(s) 11
HaeIII GGCC 3 cut(s) 66, 137, 357
Hin1II CATG 2 cut(s) 49, 269
HinfI GANTC 2 cut(s) 233, 286
Hpy188III TCNNGA 3 cut(s) 147, 333, 374
HpyAV CCTTC 4 cut(s) 148, 304, 330, 371
HpyCH4III ACNGT 2 cut(s) 126, 324
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 302, 320
HpyF3I CTNAG 2 cut(s) 118, 158
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 2 cut(s) 49, 269
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 8 cut(s) 41, 73, 108, 161, 206, 259, 306, 318
Lsp1109I GCAGC 1 cut(s) 63
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 33
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 2 cut(s) 82, 164
MfeI CAATTG 1 cut(s) 305
MhlI GDGCHC 2 cut(s) 27, 204
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 2 cut(s) 168, 305
MluNI TGGCCA 1 cut(s) 357
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 2 cut(s) 77, 115
Mox20I TGGCCA 1 cut(s) 357
MscI TGGCCA 1 cut(s) 357
MseI TTAA 2 cut(s) 279, 391
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 76
MunI CAATTG 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 38
NdeII GATC 1 cut(s) 87
NheI GCTAGC 1 cut(s) 347
NlaIII CATG 2 cut(s) 49, 269
NspI RCATGY 1 cut(s) 269
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 34
PkrI GCNGC 1 cut(s) 78
PleI GAGTC 1 cut(s) 280
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 2 cut(s) 339, 380
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 2 cut(s) 339, 380
PspCI CACGTG 1 cut(s) 34
PspPI GGNCC 2 cut(s) 339, 380
PspPPI RGGWCCY 2 cut(s) 339, 380
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 279, 391
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 2 cut(s) 339, 380
ScaI AGTACT 1 cut(s) 123
SchI GAGTC 1 cut(s) 280
SduI GDGCHC 2 cut(s) 27, 204
SetI ASST 6 cut(s) 27, 36, 195, 344, 353, 385
SinI GGWCC 2 cut(s) 339, 380
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 2 cut(s) 168, 305
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 27
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 126, 324
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 21
TasI AATT 2 cut(s) 168, 305
TatI WGTACW 1 cut(s) 121
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 279, 391
Tru9I TTAA 2 cut(s) 279, 391
TscAI CASTG 1 cut(s) 67
TseI GCWGC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 1 cut(s) 67
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 339, 380
XapI RAATTY 1 cut(s) 168
XceI RCATGY 1 cut(s) 269
XspI CTAG 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.