Rw5G010620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0021419
Physical Location & Seq
Reverse (-)
11660226 .. 11660817
592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010620.1

Sequence Viewer

Length: 453 bp
ATGGGGTCATTGTGCGTAATCGAGCTCCAGCTCGTGGCATTCATCATGGTGTTCTCTGCCAGTGGCCTCGTCCCGCTCCTTGATTTGATCTTCCCTGCTTTCGCCTCTGCCTATCTCTTAGTACTGTCGTGCTTGGCCTTCCCATCACATGGCCTTGTCTCTTCTGTGATTGTGGGAACTACGATTGGACTGTTCTTGCCTTTAGCCTATGTGTTGGGTGGTTTTGCAAGGGGTGATGATTACGCTATTCTTACTGAAAATGTCATAAGTGGGTTGTCAGTGTTTTCGCCACCAGTTAGAGCACCCATGTCGTTGCTTTACAGAGTTAGGAGAATCTTTGTCATCCTTGATTGGATATGTGAACTTGATGTTGCACGTTTTTGTTTGGAAGGACCTTGGCTAGCTGTGGCCAACTTCTTGTATTTCTTGAAGGACCTTTGGTTTAATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.5

Weight (kDa)

4.83

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 8 - 148 3.8e-27 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021419)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015761 RchiOBHm_Chr5g0015841 RchiOBHm_Chr5g0015851
rosa_samantha Rh5CG132500
rosa_wichuraiana Rw5G010620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 149
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 408
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 34, 149
AgsI TTSAA 1 cut(s) 430
AluBI AGCT 3 cut(s) 25, 31, 404
AluI AGCT 3 cut(s) 25, 31, 404
Alw21I GWGCWC 2 cut(s) 27, 304
Alw26I GTCTC 1 cut(s) 163
AoxI GGCC 4 cut(s) 64, 135, 151, 408
AspS9I GGNCC 2 cut(s) 392, 433
AsuHPI GGTGA 1 cut(s) 245
AsuNHI GCTAGC 1 cut(s) 400
AvaII GGWCC 2 cut(s) 392, 433
BalI TGGCCA 1 cut(s) 410
BanII GRGCYC 1 cut(s) 27
BauI CACGAG 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 27, 304
BccI CCATC 1 cut(s) 151
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 449
BmcAI AGTACT 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 392, 433
BmgT120I GGNCC 2 cut(s) 392, 433
BmtI GCTAGC 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 395
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 149
Bse1I ACTGG 2 cut(s) 60, 293
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 34, 149
BseNI ACTGG 2 cut(s) 60, 293
BshFI GGCC 4 cut(s) 66, 137, 153, 410
BsiHKAI GWGCWC 2 cut(s) 27, 304
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 34, 149
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 4 cut(s) 66, 137, 153, 410
Bsp1286I GDGCHC 2 cut(s) 27, 304
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 1 cut(s) 74
BspANI GGCC 4 cut(s) 66, 137, 153, 410
BspOI GCTAGC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 76
BsrI ACTGG 2 cut(s) 60, 293
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 395
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 126, 192
Bst6I CTCTTC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 1 cut(s) 90
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 449
BsuRI GGCC 4 cut(s) 66, 137, 153, 410
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 67, 285
Cac8I GCNNGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 392, 433
Csp6I GTAC 1 cut(s) 122
CviAII CATG 3 cut(s) 46, 149, 307
CviJI RGCY 9 cut(s) 25, 31, 66, 137, 153, 206, 400, 404, 410
CviKI_1 RGCY 9 cut(s) 25, 31, 66, 137, 153, 206, 400, 404, 410
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 1 cut(s) 118
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 392, 433
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 392, 433
EcoT14I CCWWGG 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 3 cut(s) 49, 152, 310
FaiI YATR 6 cut(s) 47, 150, 210, 266, 308, 358
FaqI GGGAC 1 cut(s) 56
FatI CATG 3 cut(s) 45, 148, 306
FauI CCCGC 1 cut(s) 81
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 27
FspBI CTAG 1 cut(s) 401
GsuI CTGGAG 1 cut(s) 11
HaeIII GGCC 4 cut(s) 66, 137, 153, 410
Hin1II CATG 3 cut(s) 49, 152, 310
HinfI GANTC 1 cut(s) 333
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 362
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 1 cut(s) 362
HpyAV CCTTC 3 cut(s) 148, 383, 424
HpyCH4III ACNGT 2 cut(s) 126, 192
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 2 cut(s) 227, 374
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 3 cut(s) 49, 152, 310
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 2 cut(s) 30, 81
LpnPI CCDG 4 cut(s) 41, 73, 108, 306
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 376
MalI GATC 1 cut(s) 89
MbiI CCGCTC 1 cut(s) 76
MboI GATC 1 cut(s) 87
MboII GAAGA 2 cut(s) 82, 153
MhlI GDGCHC 2 cut(s) 27, 304
MlsI TGGCCA 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 410
MnlI CCTC 2 cut(s) 77, 115
Mox20I TGGCCA 1 cut(s) 410
MscI TGGCCA 1 cut(s) 410
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 410
Mva1269I GAATGC 1 cut(s) 38
NdeII GATC 1 cut(s) 87
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 3 cut(s) 49, 152, 310
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 333
PflMI CCANNNNNTGG 2 cut(s) 34, 149
PpuMI RGGWCCY 2 cut(s) 392, 433
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 2 cut(s) 392, 433
PspPI GGNCC 2 cut(s) 392, 433
PspPPI RGGWCCY 2 cut(s) 392, 433
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 444
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 2 cut(s) 392, 433
ScaI AGTACT 1 cut(s) 123
SduI GDGCHC 2 cut(s) 27, 304
SetI ASST 6 cut(s) 27, 33, 379, 397, 406, 438
SfcI CTRYAG 1 cut(s) 449
SinI GGWCC 2 cut(s) 392, 433
SmiMI CAYNNNNRTG 1 cut(s) 47
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 401
SstI GAGCTC 1 cut(s) 27
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 2 cut(s) 126, 192
TaiI ACGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 21
TatI WGTACW 1 cut(s) 121
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 67, 285
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 2 cut(s) 67, 285
Van91I CCANNNNNTGG 2 cut(s) 34, 149
VpaK11BI GGWCC 2 cut(s) 392, 433
XspI CTAG 1 cut(s) 401
ZrmI AGTACT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.