Rh5DG406300

ABC transporter G family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013449
Physical Location & Seq
Forward (+)
64215120 .. 64236681
21562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG406300.1

Sequence Viewer

Length: 5202 bp
ATGTTCAGCAGAGAGCACCTCTTTCTCTCTCTTAGGGTTCTTCTCCATGTGCTCGCTCTCAGCCTTGTTTGGCCCCCGGCGCCTCGTGTTTTCAGCCAGGACGTCGGCGGCGGCGATGGCGGTGGCGGAGGCAATGGCGATGGCGGAGGCGGAGGCAATGGCGATGGCGGAGGCGGAGGTGATGTCGATGTCGGTGGTGGTGGAGGAGGAGACGCAGTCTCTGCCGGGTATAAGGGCTTCACCACCGAACTCATCTTGAGTCGTTTCTCCAATGTCACCCCGATTCTCAAAGATGAACTCAAGGATTCGTTCAGCTACTGCGTCATTGACGTAGACGAGGATTGGAATGGGGCTTTCAATTTCTCAGACGATGCAACATTCATTACCAACTGCGCCAAAAAAATGAAAGGTGGAGGTTTGGGAAAGATTACGCAGCGGTTGTGCACTTTTAAAGAATTATCGATGTATGGTGAAAGTTTAGTACAAGTCAAGAAAAGCAGTAAATTGAAACCGAATAAGAACTGTAACCTGTCTACATGGAATCCTGGATGTGAACCTGGGTGGGCTTGTGAAACTACTGAGGAAGTTGACGTGAAAAATAAAACATATGTGCCTGCCAGAACTGAAGACTGTGCACCTTGTTGTGAGGGTTTTTTCTGCCCTCATGGTCTTACTTGCATGATCCCTTGCCCAAAAGGTGCTTACTGCCCACTTGGAAAGCTCAATAGCACTACTGGTATATGCGTGCCATACCGTTATCACCTACCTCCAGGGAAGTCAAACCACACTTGTGGAGGAGCAGATAGGTGGGCTGATGTCTTGAGTAGTAAAGAGGTATTCTGTTCGGGTGGATCATACTGTCCGTCTAGCATCCAAAAAAATCCTTGCAGTAGTGGACATTATTGCAGGCTGGGCTCCACATCTCAGGAAGGATGTTTCAAAATGGCAAGTTGTAATGCGAAATCAGAAAATCAAAATATTACTGCATATGGTATCATGCTTTTTGCTGCAATAATAGTTATACTTATTATTATATATAACTGTACTGATCAAGTGCTTGCCACCCGAGAGAAAAGACAAGCAAAATCCAGGGAAAAGGCTGTTCAAAGTGTAAGAGAAACAGCACAAGCACGTGAAAAATGGAAATCTGCAAAAGACATTGCTAAGAAGCATGCTGTTGGACTCTCAACACAGTTTTCACGTACATTTTCTCGCAGAAAATCCACCAGGCATTCGGACCAGCTGAAAGGAACAGGACAAGCTAAACCTGGAACAGATGGTGCCTTGCCACCTATGCCACTGACTGCGATAGGTGCATCTGAAGCAGCAACATCTAAAGGAAAGAAAAAGGAAAAAAGCAACCTCACACAAATGTTACATGCTATTGAGGATGACCCAGATAGTAATGAAGGCTTTAATCTAGAGATAGGTGATAAAAATATTAAAAAGCACGCACCAAAAGGTAAGCAGTTGCATACCCAAAGCCAGATTTTCAAGTATGCATATGGTCAAATTGAGAAGGAAAAGGCTTTACAGGAGCAGACAGCAAACTTAACCTTCTCTGGGGTTATACAAATGGCTGGTGGAATTGAAATCCAAAAGAGGCCTCCAATTGAGGTTGCTTTTAAAGATCTAACCCTCACATTGAAAGGGAAAAATTTACATCTTATGAGATGTGTGACTGGGAAAATATCACCAGGCCGGGTTTCAGCTGTCATGGGTCCATCAGGAGCAGGAAAAACAACTTTTCTTAATGCTTTGTTAGGAAAAGTGAGAGGGTGCACCATGAGTGGTATGGTTCTTGTAAATGGAAAGATGGAATCTATCAACTCATATAAGAAAATCATAGGCTTTGTTCCACAAGATGATATTGTGCATGGCAATTTGACAGTGGAAGAAAATCTCTGGTTCAGCGCAAGATGCAGACTCTCTTCCAAACTGCCTCAACCAGAAAAGGTCCTGGTTGTTGAAAGAGTTATTGAATCCTTGGGCTTGCAGGCGGTGCGGGATTCCCTGGTTGGAACGGTAGAGAAGCGAGGAATCTCTGGAGGTCAAAGAAAAAGAGTAAATGTTGGGCTGGAAATGGTCATGGAGCCATCACTTCTAATTTTAGATGAACCCACATCTGGTTTGGATAGTTCATCTTCTAATCTGCTACTTAGAGCTCTTAGACGTGAGGCTCTTGAAGGAGTTAACATTTGCATGGTTGTCCACCAGCCCAGCTACACCTTGTTCAGGATGTTTGATGATTTGATACTTCTCGCTAAAGGTGGGCTTACAGTGTATCATGGATCAGTGAAGAAAGTTGAAGAGTACTTCAAGAGCCTTGGGATTATTGTTCCTGATCGTGTCAATCCTCCAGACTATTTCATTGACATTTTGGAAGGCTTAGTAAAACCAAGCACAAGCTCAGGGGTGGACTATAAACAACTACCCGTTAGGTGGATGCTTCATAATGGGTACCCAGTTCCTATGGACATGCTTCAGACTTCTGAGGGAATGGCTGCATCAACAAGTGACAGTTCAGCCCATGATTCTGGATCGGAGGGTTTTTGGCAGGATGTCAAGTGTAATGTTGAGGTGAAAAAGGACAATATACAGCATAATCTCTTAAAGTCAAGTGACTTATCGGAGCGGATTACCCCTGGCATGTTCCTGCAATATAGATACTTCCTTGGCAGGGTTGGTAAGCAGCGATTACGAGAATCGAGGACACAAGCAATAGATTTTCTTATTTTATTGCTTGCTGGATCATGCTTAGGAGTTCTTGCTAAAGTAAGCGAGGAGACCTTCGGTTATCATGGTTATATGTACACCGTCATAGCAGTTTCTCTCCTTGGCAAGATTGCAGCTTTGAGAACGTTTGGACTGGATAAGTTGCACTACTGGAGAGAGAGCTCTGCTGGCATGAGTAGCTTGGCTTACTTTCTTTCAAAAGATACAGTGGACCATTTTAATACGGTCATCAAGCCTCTCGTTTACCTCTCCATGTTCTATTTCTTCAACAATCCAAGATCATCTGTCACAGATAATTATGTCGTTTTGTTATGTTTGGTTTACTGCGTAACTGGTATAGCTTATGCCTTGTCCATATACCTGGCTCCCGGTCCAGCTCAACTGTGGTCCGTGCTTCTCCCGGTCGTTTTGACTCTAGTAGGAAACAATACTATTGAAAGCCATATTGTGGAAAAAATATCTAACTTCTGCTATACTAAGTGGGCTTTGGAATCTTTTCTCGTAGCAAATGCTCAAAAGTACTCTGGGGTGTGGATAATAACAAGATGTGCTACACTTCAGAAGAGTCACTATGACCTCAAGAATTGGCGCCCTAATTTAGCCATGCTCATTACTCTGGGTGTATTAACAAGAGTCATTGCATACATTATTTTGGTCTTCAAAAAGAAAGGGCTCGAGATAAGCTGCAGTGAGGTTTTGATAATGTCTAGCCCAAGAAGCTTGTCTGTTCGCACACCATCTGAGATTAGAAAGAAGAAGGTCGGTGGGCAGAGAAACCAAGAGGAGCTTGAAAGAGAGGTCTCTTTCCTTCAAAGACTGTTAAATCAAGAAGAGAAGGTACATGAGGTTTTGGACTATGCCTACAGTCGGAAAACTTCCGAGTCTGCTTCTGCTATTCGAATTCCAAGATTCCTTCCCCAGAAGACGAAGGAACTTTTAGCAGAGTTGGCTATGGTTGAAGGAGAAATTTCTCGACTTGAAGACCAAATCAGACAACTTAAACTAGGACTGCAACAGGAAGAACAAGCTACTAAGGAATCAAAGGCTCGACAATGGCAACATGATGAAAACCTAAGCCTTATGAACAGAGGTGTTCATCATGAAAGAAAAGGATATGAAACCAAGGCATTGCATTTCATAAGTAAAGCTATAAAGGGTGATTATGAACTTAATAATGAACTCAATTTAAATGAGAAAATGGAAAACTTGAGAAGTTTTTCTGATCAGAAAGAAAATTACTTCTATGAGGAGGTTAGACTTCAGTCTATGGTTCCACGAAAAGGTGGATTGTTAAGAGCACCCTCACCCTTGCGAACTCTGAGACATCCATCAGCACCAAAGATGAGAGAAGTTAAACCAGGCATTTCTTCAGATCATCTCCCACCAAAACCTGTACCAATTCCAACACAATTAGAAGAGAACAACCAGATTTGGCAACCAAACAAGCTCTCAGAGGAAATTTTGAAGTGTCTCAACCTCATTTATGTTAGGCTGCTTAGAACAACCAGAACAATGGAATTGGAGAAATCAGGCCCCATTTCCAGGTCTTTGCACTCTTCTGTTGTAACCTCAAGGAGCTTCAGGGATGATACCAGCCTAACCTCCAAGTCAAGCCTTCTGTTACAGAAGGAATCAAGGCAACAAGACCCTTATGGTATCTTCAATGTGGAAGACTCTATTCCTAGGGATATTGGCCCTTACAAGAACTTGGTTGTTTTTACCTCAAATTCTATGGACCCAAGATCTATTTCAGCATCGAACTCTATTCCTTTACTAAAAAAGTTGAGGGTATTGCTGAACAATCTCCTGGTTGTGAATTTGGGGTCCTTGGCATACCAGCAGAAGTTAGCATTCTGGATCAACATGTACAATGCTTGTATCATGAATGGCTTTCTCGAATTTGGTGCGCATCCTTCTGCAGACAAATTACTTACACTACTGAACAAGGCAACACTGAACATAGGAGGTAGGATTTTAAATGCTCAATCTATTGAGCATCATATACTGAGGAAACCAGCACCTTCAATTTCGATGAAAGAGGCTTTCCAGAAGGGTGAGAATGATGATGAGGCCACTGTTCGCCGCCTCTATGGTCTTGAGTCCATGGATCCTAATGTCACATTTGCTCTGTGTTGTGGAACTCGTTCTTCTCCAGCAGTGAAGATATACACAGCTGATAGTGTTGTAGCAGAGTTGGAGAAATCAAAGTTGGAGTACTTGCAAGCATCAATTGTAGTGACCAGCACAAAAAAAATTGGATTCCCGGAACTGTTGCACGGAAACATGCTTGATTTTGCTGTAGATGTGGACTCACTAGTTCAGTGGGTGTGCCACCAGTTACCTACGTCTGGGTCTTTGAGAAAATCAATGGTGGATTGCTTCAGGGGCCATAGTGGCGGGAAGATTTCTACCACTGTTGAGAAAATGCCATATGATTTTGAGTTTCATTATCTATTGGCCATGTGA

Protein Analysis

1733

Amino Acids

192.01

Weight (kDa)

8.95

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 560 - 709 2.1e-23 ABC transporter
ABC2_membrane_7 PF19055 738 - 1135 1.4e-210 ABC-2 type transporter
Lzipper-MIP1 PF14389 1169 - 1252 5e-19 Leucine-zipper of ternary complex factor MIP1
DUF547 PF04784 1521 - 1652 1.7e-35 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013449)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60720
fragaria_vesca FvH4_3g31550 FvH4_3g31550
malus_domestica MD03G1123800.v1.1 MD11G1140800.v1.1
prunus_persica Prupe.6G106400_v2.0.a1
pyrus_communis pycom11g11670
rosa_chinensis RchiOBHm_Chr5g0058191
rosa_laevigata RLG00000035202
rosa_multiflora Rmu_sc0000367.1_g000041
rosa_roxburghii Rroxscaffold_1G00022340
rosa_rugosa Rorug05G0312700 Rorug05G0312700
rosa_samantha Rh5AG380500 Rh5BG391800 Rh5CG415700 Rh5DG406300
rosa_wichuraiana Rw5G035830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 4644
Acc65I GGTACC 1 cut(s) 2460
AccB1I GGYRCC 4 cut(s) 79, 1280, 2460, 3324
AccB7I CCANNNNNTGG 1 cut(s) 3184
AccBSI CCGCTC 1 cut(s) 2635
AccI GTMKAC 2 cut(s) 333, 533
AclI AACGTT 1 cut(s) 2861
AclWI GGATC 8 cut(s) 676, 859, 2299, 2548, 2758, 4601, 4838, 4851
AcoI YGGCCR 1 cut(s) 5193
AcsI RAATTY 7 cut(s) 1657, 3636, 3702, 4194, 4462, 4552, 4634
AcuI CTGAAG 8 cut(s) 645, 1341, 2468, 3278, 3980, 4089, 4300, 5101
AcvI CACGTG 1 cut(s) 1133
AcyI GRCGYC 3 cut(s) 80, 102, 3325
AflIII ACRYGT 1 cut(s) 4599
AhlI ACTAGT 1 cut(s) 5050
AjiI CACGTC 2 cut(s) 592, 2174
AjuI GAANNNNNNNTTGG 4 cut(s) 4468, 4500, 4928, 4960
AleI CACNNNNGTG 1 cut(s) 789
Alw21I GWGCWC 8 cut(s) 18, 54, 446, 637, 1784, 2167, 2900, 4036
Alw26I GTCTC 6 cut(s) 204, 223, 2780, 3541, 4051, 4211
Alw44I GTGCAC 3 cut(s) 442, 633, 1780
AlwI GGATC 8 cut(s) 676, 859, 2299, 2548, 2758, 4601, 4838, 4851
AlwNI CAGNNNCTG 2 cut(s) 221, 318
Ama87I CYCGRG 2 cut(s) 1065, 3410
AoxI GGCC 8 cut(s) 71, 1604, 1699, 4267, 4429, 4806, 5122, 5193
ApaLI GTGCAC 3 cut(s) 442, 633, 1780
ApeKI GCWGC 8 cut(s) 433, 1007, 1325, 2504, 2692, 2849, 3420, 4228
ApoI RAATTY 7 cut(s) 1657, 3636, 3702, 4194, 4462, 4552, 4634
Asp700I GAANNNNTTC 3 cut(s) 3231, 3952, 4879
Asp718I GGTACC 1 cut(s) 2460
AspA2I CCTAGG 1 cut(s) 4418
AspLEI GCGC 5 cut(s) 82, 395, 1916, 3327, 4645
AsuC2I CCSGG 6 cut(s) 77, 226, 1703, 3105, 3137, 5000
AsuII TTCGAA 1 cut(s) 3634
AvaI CYCGRG 2 cut(s) 1065, 3410
AvaII GGWCC 8 cut(s) 1237, 1721, 1957, 2947, 3107, 3123, 4471, 4560
AvrII CCTAGG 1 cut(s) 4418
BaeGI GKGCMC 3 cut(s) 446, 637, 1784
BalI TGGCCA 1 cut(s) 5195
BamHI GGATCC 1 cut(s) 4843
BanI GGYRCC 4 cut(s) 79, 1280, 2460, 3324
BanII GRGCYC 4 cut(s) 917, 2167, 2900, 3411
BarI GAAGNNNNNNTAC 4 cut(s) 221, 253, 3558, 3590
BauI CACGAG 1 cut(s) 84
BbrPI CACGTG 1 cut(s) 1133
BbsI GAAGAC 5 cut(s) 633, 3385, 3665, 3723, 4413
Bbv12I GWGCWC 8 cut(s) 18, 54, 446, 637, 1784, 2167, 2900, 4036
BbvI GCAGC 8 cut(s) 445, 994, 1337, 2491, 2704, 2861, 3407, 4215
BccI CCATC 9 cut(s) 110, 134, 158, 1271, 1732, 1810, 2104, 3481, 4072
BcgI CGANNNNNNTGC 2 cut(s) 4622, 4656
BclI TGATCA 2 cut(s) 1048, 3958
BcnI CCSGG 6 cut(s) 77, 226, 1703, 3105, 3137, 5000
BcoDI GTCTC 6 cut(s) 204, 223, 2780, 3541, 4051, 4211
BcuI ACTAGT 1 cut(s) 5050
BfaI CTAG 7 cut(s) 867, 1421, 3152, 3444, 3740, 4419, 5051
BfmI CTRYAG 4 cut(s) 3421, 3598, 4653, 5034
BfoI RGCGCY 2 cut(s) 83, 3328
BglI GCCNNNNNGGC 1 cut(s) 5130
BglII AGATCT 2 cut(s) 1630, 4478
BlnI CCTAGG 1 cut(s) 4418
BmcAI AGTACT 3 cut(s) 2315, 3257, 4952
Bme18I GGWCC 8 cut(s) 1237, 1721, 1957, 2947, 3107, 3123, 4471, 4560
BmeT110I CYCGRG 2 cut(s) 1065, 3410
BmgBI CACGTC 2 cut(s) 592, 2174
BmrI ACTGGG 2 cut(s) 1692, 2459
BmuI ACTGGG 2 cut(s) 1692, 2459
BoxI GACNNNNGTC 1 cut(s) 3997
BpiI GAAGAC 5 cut(s) 633, 3385, 3665, 3723, 4413
BplI GAGNNNNNCTC 1 cut(s) 35
BpmI CTGGAG 5 cut(s) 753, 2067, 2343, 2908, 4872
Bpu10I CCTNAGC 3 cut(s) 2410, 2758, 3809
Bpu14I TTCGAA 1 cut(s) 3634
BpuEI CTTGAG 7 cut(s) 277, 284, 841, 3299, 3964, 4291, 4853
BpuMI CCSGG 6 cut(s) 77, 226, 1703, 3105, 3137, 5000
Bsa29I ATCGAT 1 cut(s) 461
BsaAI YACGTR 2 cut(s) 1133, 1202
BsaHI GRCGYC 3 cut(s) 80, 102, 3325
BsaI GGTCTC 2 cut(s) 2780, 3541
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 201, 231
Bse1I ACTGG 7 cut(s) 739, 1687, 2465, 2874, 2891, 3073, 5071
Bse3DI GCAATG 5 cut(s) 139, 163, 1158, 3372, 3863
BseCI ATCGAT 1 cut(s) 461
BseMI GCAATG 5 cut(s) 139, 163, 1158, 3372, 3863
BseNI ACTGG 7 cut(s) 739, 1687, 2465, 2874, 2891, 3073, 5071
BseRI GAGGAG 6 cut(s) 219, 222, 810, 2798, 3533, 3998
BseSI GKGCMC 3 cut(s) 446, 637, 1784
BseXI GCAGC 8 cut(s) 445, 994, 1337, 2491, 2704, 2861, 3407, 4215
BseYI CCCAGC 2 cut(s) 910, 2219
Bsh1285I CGRYCG 1 cut(s) 3141
BshFI GGCC 8 cut(s) 73, 1606, 1701, 4269, 4431, 4808, 5124, 5195
BshNI GGYRCC 4 cut(s) 79, 1280, 2460, 3324
BshVI ATCGAT 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 3141
BsiHKAI GWGCWC 8 cut(s) 18, 54, 446, 637, 1784, 2167, 2900, 4036
BsiHKCI CYCGRG 2 cut(s) 1065, 3410
BsiSI CCGG 6 cut(s) 77, 225, 1702, 3105, 3137, 5000
BsmAI GTCTC 6 cut(s) 204, 223, 2780, 3541, 4051, 4211
BsmBI CGTCTC 1 cut(s) 204
BsmI GAATGC 2 cut(s) 1231, 4586
BsnI GGCC 8 cut(s) 73, 1606, 1701, 4269, 4431, 4808, 5124, 5195
Bso31I GGTCTC 2 cut(s) 2780, 3541
BsoBI CYCGRG 2 cut(s) 1065, 3410
Bsp119I TTCGAA 1 cut(s) 3634
Bsp1407I TGTACA 2 cut(s) 2811, 4602
Bsp19I CCATGG 1 cut(s) 4839
BspANI GGCC 8 cut(s) 73, 1606, 1701, 4269, 4431, 4808, 5124, 5195
BspDI ATCGAT 1 cut(s) 461
BspHI TCATGA 2 cut(s) 3835, 4617
BspMAI CTGCAG 2 cut(s) 3425, 4657
BspPI GGATC 8 cut(s) 676, 859, 2299, 2548, 2758, 4601, 4838, 4851
BspT104I TTCGAA 1 cut(s) 3634
BspT107I GGYRCC 4 cut(s) 79, 1280, 2460, 3324
BspTNI GGTCTC 2 cut(s) 2780, 3541
BsrBI CCGCTC 1 cut(s) 2635
BsrDI GCAATG 5 cut(s) 139, 163, 1158, 3372, 3863
BsrGI TGTACA 2 cut(s) 2811, 4602
BsrI ACTGG 7 cut(s) 739, 1687, 2465, 2874, 2891, 3073, 5071
BssNI GRCGYC 3 cut(s) 80, 102, 3325
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 8 cut(s) 1986, 2326, 2674, 2836, 3858, 4418, 4563, 4839
Bst2BI CACGAG 1 cut(s) 84
Bst6I CTCTTC 6 cut(s) 1936, 2304, 3293, 3561, 4146, 4297
BstACI GRCGYC 3 cut(s) 80, 102, 3325
BstAPI GCANNNNNTGC 2 cut(s) 221, 2002
BstAUI TGTACA 2 cut(s) 2811, 4602
BstBAI YACGTR 2 cut(s) 1133, 1202
BstBI TTCGAA 1 cut(s) 3634
BstDSI CCRYGG 1 cut(s) 4839
BstENI CCTNNNNNAGG 1 cut(s) 2233
BstH2I RGCGCY 2 cut(s) 83, 3328
BstHHI GCGC 5 cut(s) 82, 395, 1916, 3327, 4645
BstMAI GTCTC 6 cut(s) 204, 223, 2780, 3541, 4051, 4211
BstMCI CGRYCG 1 cut(s) 3141
BstNSI RCATGY 6 cut(s) 1175, 1382, 2482, 2653, 4603, 5023
BstPAI GACNNNNGTC 1 cut(s) 3997
BstSFI CTRYAG 4 cut(s) 3421, 3598, 4653, 5034
BstSLI GKGCMC 3 cut(s) 446, 637, 1784
BstV1I GCAGC 8 cut(s) 445, 994, 1337, 2491, 2704, 2861, 3407, 4215
BstV2I GAAGAC 5 cut(s) 633, 3385, 3665, 3723, 4413
BstX2I RGATCY 3 cut(s) 1630, 4478, 4843
BstXI CCANNNNNNTGG 4 cut(s) 791, 2537, 3097, 4249
BstYI RGATCY 3 cut(s) 1630, 4478, 4843
Bsu15I ATCGAT 1 cut(s) 461
BsuRI GGCC 8 cut(s) 73, 1606, 1701, 4269, 4431, 4808, 5124, 5195
BsuTUI ATCGAT 1 cut(s) 461
BtgI CCRYGG 1 cut(s) 4839
BtgZI GCGATG 3 cut(s) 129, 153, 177
BtrI CACGTC 2 cut(s) 592, 2174
BtsI GCAGTG 2 cut(s) 3430, 4899
CaiI CAGNNNCTG 2 cut(s) 221, 318
CciI TCATGA 2 cut(s) 3835, 4617
CfoI GCGC 5 cut(s) 82, 395, 1916, 3327, 4645
ClaI ATCGAT 1 cut(s) 461
CseI GACGC 2 cut(s) 221, 310
DinI GGCGCC 2 cut(s) 81, 3326
DraI TTTAAA 4 cut(s) 451, 1627, 3924, 4713
EaeI YGGCCR 1 cut(s) 5193
Eam1104I CTCTTC 6 cut(s) 1936, 2304, 3293, 3561, 4146, 4297
EarI CTCTTC 6 cut(s) 1936, 2304, 3293, 3561, 4146, 4297
EciI GGCGGA 5 cut(s) 141, 159, 165, 183, 189
Ecl136II GAGCTC 2 cut(s) 2165, 2898
Eco130I CCWWGG 8 cut(s) 1986, 2326, 2674, 2836, 3858, 4418, 4563, 4839
Eco147I AGGCCT 1 cut(s) 1606
Eco24I GRGCYC 4 cut(s) 917, 2167, 2900, 3411
Eco31I GGTCTC 2 cut(s) 2780, 3541
Eco47I GGWCC 8 cut(s) 1237, 1721, 1957, 2947, 3107, 3123, 4471, 4560
Eco53kI GAGCTC 2 cut(s) 2165, 2898
Eco57I CTGAAG 8 cut(s) 645, 1341, 2468, 3278, 3980, 4089, 4300, 5101
Eco72I CACGTG 1 cut(s) 1133
Eco88I CYCGRG 2 cut(s) 1065, 3410
EcoICRI GAGCTC 2 cut(s) 2165, 2898
EcoNI CCTNNNNNAGG 1 cut(s) 2233
EcoO109I RGGNCCY 3 cut(s) 1957, 4268, 4560
EcoRI GAATTC 1 cut(s) 3636
EcoT14I CCWWGG 8 cut(s) 1986, 2326, 2674, 2836, 3858, 4418, 4563, 4839
EcoT22I ATGCAT 1 cut(s) 1504
EcoT38I GRGCYC 4 cut(s) 917, 2167, 2900, 3411
EgeI GGCGCC 2 cut(s) 81, 3326
EheI GGCGCC 2 cut(s) 81, 3326
ErhI CCWWGG 8 cut(s) 1986, 2326, 2674, 2836, 3858, 4418, 4563, 4839
Esp3I CGTCTC 1 cut(s) 204
FalI AAGNNNNNCTT 4 cut(s) 2259, 2291, 3507, 3539
FauI CCCGC 2 cut(s) 1998, 5126
FauNDI CATATG 4 cut(s) 607, 988, 1504, 5167
FbaI TGATCA 2 cut(s) 1048, 3958
FblI GTMKAC 2 cut(s) 333, 533
FriOI GRGCYC 4 cut(s) 917, 2167, 2900, 3411
FspAI RTGCGCAY 1 cut(s) 4644
FspBI CTAG 7 cut(s) 867, 1421, 3152, 3444, 3740, 4419, 5051
FspI TGCGCA 1 cut(s) 4644
GlaI GCGC 5 cut(s) 81, 394, 1915, 3326, 4644
GsaI CCCAGC 2 cut(s) 914, 2223
GsuI CTGGAG 5 cut(s) 753, 2067, 2343, 2908, 4872
HaeII RGCGCY 2 cut(s) 83, 3328
HaeIII GGCC 8 cut(s) 73, 1606, 1701, 4269, 4431, 4808, 5124, 5195
HapII CCGG 6 cut(s) 77, 225, 1702, 3105, 3137, 5000
HgaI GACGC 2 cut(s) 221, 310
HhaI GCGC 5 cut(s) 82, 395, 1916, 3327, 4645
Hin1I GRCGYC 3 cut(s) 80, 102, 3325
Hin6I GCGC 5 cut(s) 80, 393, 1914, 3325, 4643
HinP1I GCGC 5 cut(s) 80, 393, 1914, 3325, 4643
HincII GTYRAC 2 cut(s) 589, 2194
HindII GTYRAC 2 cut(s) 589, 2194
HindIII AAGCTT 1 cut(s) 3454
HpaI GTTAAC 1 cut(s) 2194
HpaII CCGG 6 cut(s) 77, 225, 1702, 3105, 3137, 5000
Hpy99I CGWCG 1 cut(s) 107
HpyCH4IV ACGT 8 cut(s) 102, 330, 591, 1132, 1201, 2173, 2861, 5081
HpySE526I ACGT 8 cut(s) 102, 330, 591, 1132, 1201, 2173, 2861, 5081
Hsp92I GRCGYC 3 cut(s) 80, 102, 3325
HspAI GCGC 5 cut(s) 80, 393, 1914, 3325, 4643
KasI GGCGCC 2 cut(s) 79, 3324
KpnI GGTACC 1 cut(s) 2464
Ksp22I TGATCA 2 cut(s) 1048, 3958
KspAI GTTAAC 1 cut(s) 2194
LmnI GCTCC 9 cut(s) 797, 920, 1537, 1730, 2092, 2632, 3106, 3520, 4311
Lsp1109I GCAGC 8 cut(s) 445, 994, 1337, 2491, 2704, 2861, 3407, 4215
MaeI CTAG 7 cut(s) 867, 1421, 3152, 3444, 3740, 4419, 5051
MaeII ACGT 8 cut(s) 102, 330, 591, 1132, 1201, 2173, 2861, 5081
MbiI CCGCTC 1 cut(s) 2635
MfeI CAATTG 2 cut(s) 1611, 4965
MflI RGATCY 3 cut(s) 1630, 4478, 4843
MlsI TGGCCA 1 cut(s) 5195
MluNI TGGCCA 1 cut(s) 5195
Mly113I GGCGCC 2 cut(s) 80, 3325
MmeI TCCRAC 6 cut(s) 1159, 1999, 3584, 4163, 4911, 4926
Mox20I TGGCCA 1 cut(s) 5195
Mph1103I ATGCAT 1 cut(s) 1504
MroXI GAANNNNTTC 3 cut(s) 3231, 3952, 4879
MscI TGGCCA 1 cut(s) 5195
MslI CAYNNNNRTG 3 cut(s) 789, 1370, 2201
Msp20I TGGCCA 1 cut(s) 5195
MspA1I CMGCKG 4 cut(s) 436, 1243, 1712, 4910
MspI CCGG 6 cut(s) 77, 225, 1702, 3105, 3137, 5000
MunI CAATTG 2 cut(s) 1611, 4965
Mva1269I GAATGC 2 cut(s) 1231, 4586
NarI GGCGCC 2 cut(s) 80, 3325
NciI CCSGG 6 cut(s) 77, 226, 1703, 3105, 3137, 5000
NcoI CCATGG 1 cut(s) 4839
NdeI CATATG 4 cut(s) 607, 988, 1504, 5167
NmuCI GTSAC 8 cut(s) 274, 1678, 2516, 2621, 3022, 3302, 4852, 4972
NsbI TGCGCA 1 cut(s) 4644
NsiI ATGCAT 1 cut(s) 1504
NspI RCATGY 6 cut(s) 1175, 1382, 2482, 2653, 4603, 5023
NspV TTCGAA 1 cut(s) 3634
OliI CACNNNNGTG 1 cut(s) 789
PaeI GCATGC 1 cut(s) 1175
PaeR7I CTCGAG 1 cut(s) 3410
PagI TCATGA 2 cut(s) 3835, 4617
PceI AGGCCT 1 cut(s) 1606
PciI ACATGT 1 cut(s) 4599
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 2 cut(s) 1231, 4586
PdmI GAANNNNTTC 3 cut(s) 3231, 3952, 4879
PflFI GACNNNGTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 3184
PfoI TCCNGGA 2 cut(s) 544, 4998
PluTI GGCGCC 2 cut(s) 83, 3328
PmaCI CACGTG 1 cut(s) 1133
PmlI CACGTG 1 cut(s) 1133
Ppu21I YACGTR 2 cut(s) 1133, 1202
PpuMI RGGWCCY 2 cut(s) 1957, 4560
PscI ACATGT 1 cut(s) 4599
PshAI GACNNNNGTC 1 cut(s) 3997
Psp124BI GAGCTC 2 cut(s) 2167, 2900
Psp1406I AACGTT 1 cut(s) 2861
Psp5II RGGWCCY 2 cut(s) 1957, 4560
PspCI CACGTG 1 cut(s) 1133
PspFI CCCAGC 2 cut(s) 910, 2219
PspPPI RGGWCCY 2 cut(s) 1957, 4560
PstI CTGCAG 2 cut(s) 3425, 4657
PstNI CAGNNNCTG 2 cut(s) 221, 318
PsuI RGATCY 3 cut(s) 1630, 4478, 4843
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 3 cut(s) 1243, 1712, 4910
RseI CAYNNNNRTG 3 cut(s) 789, 1370, 2201
SacI GAGCTC 2 cut(s) 2167, 2900
ScaI AGTACT 3 cut(s) 2315, 3257, 4952
SfcI CTRYAG 4 cut(s) 3421, 3598, 4653, 5034
SfoI GGCGCC 2 cut(s) 81, 3326
Sfr274I CTCGAG 1 cut(s) 3410
SfuI TTCGAA 1 cut(s) 3634
SinI GGWCC 8 cut(s) 1237, 1721, 1957, 2947, 3107, 3123, 4471, 4560
SlaI CTCGAG 1 cut(s) 3410
SmiI ATTTAAAT 1 cut(s) 3924
SmiMI CAYNNNNRTG 3 cut(s) 789, 1370, 2201
SmlI CTYRAG 8 cut(s) 256, 299, 820, 3314, 3410, 3943, 4306, 4832
SmoI CTYRAG 8 cut(s) 256, 299, 820, 3314, 3410, 3943, 4306, 4832
SpeI ACTAGT 1 cut(s) 5050
SphI GCATGC 1 cut(s) 1175
SseBI AGGCCT 1 cut(s) 1606
SspDI GGCGCC 2 cut(s) 79, 3324
SspI AATATT 2 cut(s) 979, 1441
SspMI CTAG 7 cut(s) 867, 1421, 3152, 3444, 3740, 4419, 5051
SstI GAGCTC 2 cut(s) 2167, 2900
StuI AGGCCT 1 cut(s) 1606
StyI CCWWGG 8 cut(s) 1986, 2326, 2674, 2836, 3858, 4418, 4563, 4839
SwaI ATTTAAAT 1 cut(s) 3924
TaiI ACGT 8 cut(s) 105, 333, 594, 1135, 1204, 2176, 2864, 5084
TatI WGTACW 7 cut(s) 481, 1043, 2313, 2811, 3255, 4602, 4950
TauI GCSGC 3 cut(s) 111, 114, 4821
TseFI GTSAC 8 cut(s) 274, 1678, 2516, 2621, 3022, 3302, 4852, 4972
TseI GCWGC 8 cut(s) 433, 1007, 1325, 2504, 2692, 2849, 3420, 4228
Tsp45I GTSAC 8 cut(s) 274, 1678, 2516, 2621, 3022, 3302, 4852, 4972
TspGWI ACGGA 3 cut(s) 852, 3115, 5028
Tth111I GACNNNGTC 1 cut(s) 215
Van91I CCANNNNNTGG 1 cut(s) 3184
VneI GTGCAC 3 cut(s) 442, 633, 1780
VpaK11BI GGWCC 8 cut(s) 1237, 1721, 1957, 2947, 3107, 3123, 4471, 4560
XagI CCTNNNNNAGG 1 cut(s) 2233
XapI RAATTY 7 cut(s) 1657, 3636, 3702, 4194, 4462, 4552, 4634
XbaI TCTAGA 1 cut(s) 1420
XceI RCATGY 6 cut(s) 1175, 1382, 2482, 2653, 4603, 5023
XcmI CCANNNNNNNNNTGG 3 cut(s) 1792, 2128, 3117
XhoI CTCGAG 1 cut(s) 3410
XmaJI CCTAGG 1 cut(s) 4418
XmiI GTMKAC 2 cut(s) 333, 533
XmnI GAANNNNTTC 3 cut(s) 3231, 3952, 4879
XspI CTAG 7 cut(s) 867, 1421, 3152, 3444, 3740, 4419, 5051
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 3 cut(s) 2315, 3257, 4952
Zsp2I ATGCAT 1 cut(s) 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.