Rh5DG476600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0021442
Physical Location & Seq
Forward (+)
75276789 .. 75289201
12413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG476600.1

Sequence Viewer

Length: 408 bp
ATGAGAGTCAGGAAAGAGCTTGAAAAGGCAGAGCGGAGAGCCACCGCACAGTTAATGTTCGACTTGGGACAGAAGGCTTATGGGAGGGGGATGTACGGTCGTGCCTTTGAGTTCCTAGAACGAGCACTGACAATCATTCCGAGGCCAACGTTATTTGGTGGTGAGATTCAAATTTGGCTTGCTATGGCCTACAAGGCTAATAAGCGCCATGTCGAATGCATTGATCTTTATAAGCAATTGGAGAACAAGCACCCAAGTGTCAGCTTCCCGTGCCAGGCTGCAGAGCTTCGCCACATTTTCCAAGCACCAAAGATCAAGATTACTCGAGAAGAGATGATGCTTCTTGATCAGCTGGCCATGATATGCTCAAAGAAGGAAATCTTTGTTTTTTCAGTCAATTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.83

Weight (kDa)

9.57

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021442)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0067811
rosa_multiflora Rmu_sc0000435.1_g000054
rosa_samantha Rh5CG484400 Rh5DG476600
rosa_wichuraiana Rw5G041570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 231, 402
AccBSI CCGCTC 1 cut(s) 34
AciI CCGC 2 cut(s) 34, 45
AclI AACGTT 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 171
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 274
AgsI TTSAA 2 cut(s) 23, 170
AjnI CCWGG 1 cut(s) 273
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 4 cut(s) 19, 264, 286, 352
AluI AGCT 4 cut(s) 19, 264, 286, 352
Alw21I GWGCWC 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 3 cut(s) 143, 186, 354
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 171
AspLEI GCGC 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 324
BalI TGGCCA 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 265
BciT130I CCWGG 1 cut(s) 275
BclI TGATCA 1 cut(s) 346
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 208
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
Bme1390I CCNGG 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 274
BseXI GCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 3 cut(s) 145, 188, 356
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 81
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 3 cut(s) 145, 188, 356
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 223, 312, 346
BspACI CCGC 2 cut(s) 34, 45
BspANI GGCC 3 cut(s) 145, 188, 356
BspMAI CTGCAG 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 3 cut(s) 223, 312, 346
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 51, 98
Bst6I CTCTTC 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 180, 354
BstF5I GGATG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 226, 315, 349
BstMBI GATC 3 cut(s) 223, 312, 346
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 194, 270
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 273
BstSFI CTRYAG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 3 cut(s) 145, 188, 356
BtsCI GGATG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 180, 354
CfoI GCGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 94
CviAII CATG 2 cut(s) 209, 358
CviQI GTAC 1 cut(s) 94
DpnI GATC 3 cut(s) 225, 314, 348
DpnII GATC 3 cut(s) 223, 312, 346
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 221
FaeI CATG 2 cut(s) 212, 361
FaiI YATR 7 cut(s) 81, 185, 210, 231, 359, 364, 402
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 1 cut(s) 81
FatI CATG 2 cut(s) 208, 357
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 206
GluI GCNGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 3 cut(s) 145, 188, 356
HhaI GCGC 1 cut(s) 207
Hin1II CATG 2 cut(s) 212, 361
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 2 cut(s) 6, 166
HphI GGTGA 1 cut(s) 173
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 4 cut(s) 10, 316, 326, 344
HpyAV CCTTC 2 cut(s) 67, 367
HpyCH4III ACNGT 2 cut(s) 51, 98
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 219, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 270
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 2 cut(s) 212, 361
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 223, 312, 346
LpnPI CCDG 3 cut(s) 260, 287, 338
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 1 cut(s) 327
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 149
MalI GATC 3 cut(s) 225, 314, 348
MbiI CCGCTC 1 cut(s) 34
MboI GATC 3 cut(s) 223, 312, 346
MboII GAAGA 1 cut(s) 341
MfeI CAATTG 1 cut(s) 236
MhlI GDGCHC 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 3 cut(s) 171, 236, 397
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 2 cut(s) 78, 135
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 221
MscI TGGCCA 1 cut(s) 356
MseI TTAA 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 255
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 352
MspR9I CCNGG 1 cut(s) 275
MunI CAATTG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 194, 270
NdeII GATC 3 cut(s) 223, 312, 346
NlaIII CATG 2 cut(s) 212, 361
NsiI ATGCAT 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 324
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 166
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PsiI TTATAA 2 cut(s) 231, 402
Psp1406I AACGTT 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PstI CTGCAG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 352
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 3 cut(s) 223, 312, 346
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 127
SetI ASST 5 cut(s) 21, 152, 266, 288, 354
SfaNI GCATC 1 cut(s) 327
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
Sse9I AATT 3 cut(s) 171, 236, 397
SsiI CCGC 2 cut(s) 34, 45
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 51, 98
TaiI ACGT 1 cut(s) 152
TaqI TCGA 3 cut(s) 60, 213, 325
TasI AATT 3 cut(s) 171, 236, 397
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 278
TspRI CASTG 1 cut(s) 132
XapI RAATTY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 324
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.