Rh6AG312100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0020590
Physical Location & Seq
Reverse (-)
51143874 .. 51144671
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG312100.1

Sequence Viewer

Length: 798 bp
ATGTCTCTCGAGCTGTTAGCCTTCTCCTCCCTACTCATCCTCCCCTTGCTTTTCTTCATCCTCAAGCTTTCCGGCAACTCGAAAACCAACAGCAACAGCAAGCTCCCCAAATCATACCCAATAATTAGTTCATTCCCAGCCCTCTACAAGAACCATCCCAATAAACTCCCATGGTTCACTGAGCTTCTCCAAGACTCGCCCACATCTACTATTACTATGCACCATTCTCTGGGCTGCCACTTCGTCGTCACCGCCAACTCGGCCGTGGTCCAGCACATCCTCAAGACAAAGTTCCACATCTACCAAAAGGGCGAGTCCTTCCGTACAACTCTCCACGACCTCCTTGGCGATGGGATTTTCAACGCCGACGGTGACAATTGGAAGTTCCAGCGGCAACTCTCCAGCCATGAATTCAACACAAAGTCCCTCAGGAAATTTGTGGAAGACGTGGTGGGCACTGAGCTCTACGATCGGCTGATCCCCATTCTATCCAAATCTTCCAGCGACCGAACCATTCTCGATTTCCAAGACATTCTCCAGCGGTTCGCCTTTGACAACATTTGCCGGATCGGGTTCGGGTACGACCCGGAGTATCTCCTTCCTTCTCTGCTACAAGCTAAATTCGCGGTGGCGTTCGACAATGAGGTCCAGATCAGCAGCGAAAGGTTCAGTTCCATGCGCCCGGTGTGGAGGATCAAACGAGCTCTCGGAATCGGATCGGAAAGCCATCTCCGCTGTGTGGTCTCTAACGTCCGAGAGTTTGCGAACACCATCGATCGTCAAGCAAAAGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

265

Amino Acids

30.19

Weight (kDa)

8.91

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 63 - 202 1.7e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020590)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0268161
rosa_rugosa Rorug06G0039300
rosa_samantha Rh6AG159200 Rh6AG312100 Rh6BG319000 Rh6CG154800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
AccB7I CCANNNNNTGG 1 cut(s) 229
AccII CGCG 1 cut(s) 626
AciI CCGC 5 cut(s) 252, 391, 541, 626, 733
AclWI GGATC 4 cut(s) 472, 575, 701, 724
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 3 cut(s) 410, 434, 620
AfaI GTAC 2 cut(s) 325, 581
AfiI CCNNNNNNNGG 2 cut(s) 229, 739
AgsI TTSAA 2 cut(s) 361, 415
AjiI CACGTC 1 cut(s) 448
AjuI GAANNNNNNNTTGG 2 cut(s) 112, 144
AluBI AGCT 7 cut(s) 13, 67, 103, 184, 463, 617, 704
AluI AGCT 7 cut(s) 13, 67, 103, 184, 463, 617, 704
Alw21I GWGCWC 2 cut(s) 465, 706
Alw26I GTCTC 2 cut(s) 9, 748
AlwI GGATC 4 cut(s) 472, 575, 701, 724
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 1 cut(s) 261
ApeKI GCWGC 2 cut(s) 234, 657
ApoI RAATTY 3 cut(s) 410, 434, 620
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 2 cut(s) 268, 646
AsuC2I CCSGG 2 cut(s) 587, 683
AsuHPI GGTGA 2 cut(s) 241, 383
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 268, 646
AxyI CCTNAGG 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 458
BanII GRGCYC 2 cut(s) 465, 706
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 2 cut(s) 465, 706
BbvI GCAGC 2 cut(s) 221, 669
BccI CCATC 4 cut(s) 162, 344, 735, 779
BceAI ACGGC 1 cut(s) 248
BcnI CCSGG 2 cut(s) 587, 683
BcoDI GTCTC 2 cut(s) 9, 748
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 3 cut(s) 235, 392, 658
BlsI GCNGC 3 cut(s) 236, 393, 659
Bme1390I CCNGG 2 cut(s) 587, 683
Bme18I GGWCC 2 cut(s) 268, 646
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 268, 646
BmrFI CCNGG 2 cut(s) 587, 683
BmsI GCATC 1 cut(s) 781
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 2 cut(s) 385, 521
BpuEI CTTGAG 2 cut(s) 47, 266
BpuMI CCSGG 2 cut(s) 587, 683
Bsa29I ATCGAT 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 748
BsaJI CCNNGG 3 cut(s) 170, 264, 343
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 739
Bse21I CCTNAGG 1 cut(s) 428
BseCI ATCGAT 1 cut(s) 774
BseDI CCNNGG 3 cut(s) 170, 264, 343
BseGI GGATG 4 cut(s) 36, 57, 154, 276
BseLI CCNNNNNNNGG 2 cut(s) 229, 739
BseMII CTCAG 3 cut(s) 171, 442, 450
BseRI GAGGAG 1 cut(s) 16
BseSI GKGCMC 1 cut(s) 458
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 2 cut(s) 221, 669
BseYI CCCAGC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 626
Bsh1285I CGRYCG 4 cut(s) 264, 472, 508, 778
BshFI GGCC 1 cut(s) 263
BshVI ATCGAT 1 cut(s) 774
BsiEI CGRYCG 4 cut(s) 264, 472, 508, 778
BsiHKAI GWGCWC 2 cut(s) 465, 706
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 72, 565, 587, 683
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 2 cut(s) 229, 739
BsmAI GTCTC 2 cut(s) 9, 748
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 1 cut(s) 263
Bso31I GGTCTC 1 cut(s) 748
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 458, 465, 706
Bsp143I GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
Bsp19I CCATGG 1 cut(s) 170
BspACI CCGC 5 cut(s) 252, 391, 541, 626, 733
BspANI GGCC 1 cut(s) 263
BspCNI CTCAG 3 cut(s) 172, 441, 451
BspDI ATCGAT 1 cut(s) 774
BspFNI CGCG 1 cut(s) 626
BspPI GGATC 4 cut(s) 472, 575, 701, 724
BspTNI GGTCTC 1 cut(s) 748
BssECI CCNNGG 3 cut(s) 170, 264, 343
BssMI GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
BssT1I CCWWGG 2 cut(s) 170, 343
Bst4CI ACNGT 1 cut(s) 371
BstAPI GCANNNNNTGC 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 3 cut(s) 180, 428, 459
BstDSI CCRYGG 2 cut(s) 170, 264
BstF5I GGATG 4 cut(s) 36, 57, 154, 276
BstFNI CGCG 1 cut(s) 626
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 7 cut(s) 472, 480, 570, 654, 696, 719, 778
BstMAI GTCTC 2 cut(s) 9, 748
BstMBI GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
BstMCI CGRYCG 4 cut(s) 264, 472, 508, 778
BstMWI GCNNNNNNNGC 4 cut(s) 260, 623, 732, 791
BstSCI CCNGG 2 cut(s) 585, 681
BstSLI GKGCMC 1 cut(s) 458
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 2 cut(s) 221, 669
BstV2I GAAGAC 1 cut(s) 450
BstZI CGGCCG 1 cut(s) 261
Bsu15I ATCGAT 1 cut(s) 774
Bsu36I CCTNAGG 1 cut(s) 428
BsuRI GGCC 1 cut(s) 263
BsuTUI ATCGAT 1 cut(s) 774
BtgI CCRYGG 2 cut(s) 170, 264
BtgZI GCGATG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 4 cut(s) 36, 57, 154, 276
BtsIMutI CAGTG 2 cut(s) 177, 456
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 681
Cfr13I GGNCC 2 cut(s) 268, 646
ClaI ATCGAT 1 cut(s) 774
Csp6I GTAC 2 cut(s) 324, 580
CviAII CATG 4 cut(s) 171, 407, 676, 795
CviQI GTAC 2 cut(s) 324, 580
DdeI CTNAG 3 cut(s) 180, 428, 459
DpnI GATC 7 cut(s) 471, 479, 569, 653, 695, 718, 777
DpnII GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 1 cut(s) 261
EagI CGGCCG 1 cut(s) 261
Ecl136II GAGCTC 2 cut(s) 463, 704
EclXI CGGCCG 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 170, 343
Eco24I GRGCYC 2 cut(s) 465, 706
Eco31I GGTCTC 1 cut(s) 748
Eco47I GGWCC 2 cut(s) 268, 646
Eco52I CGGCCG 1 cut(s) 261
Eco53kI GAGCTC 2 cut(s) 463, 704
Eco81I CCTNAGG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 463, 704
EcoRI GAATTC 1 cut(s) 410
EcoT14I CCWWGG 2 cut(s) 170, 343
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 2 cut(s) 465, 706
ErhI CCWWGG 2 cut(s) 170, 343
FaeI CATG 4 cut(s) 174, 410, 679, 798
FaiI YATR 6 cut(s) 115, 172, 218, 408, 677, 796
FaqI GGGAC 1 cut(s) 409
FatI CATG 4 cut(s) 170, 406, 675, 794
Fnu4HI GCNGC 3 cut(s) 235, 392, 658
FokI GGATG 4 cut(s) 23, 44, 141, 263
FriOI GRGCYC 2 cut(s) 465, 706
Fsp4HI GCNGC 3 cut(s) 235, 392, 658
GlaI GCGC 1 cut(s) 680
GluI GCNGC 3 cut(s) 235, 392, 658
GsaI CCCAGC 1 cut(s) 140
GsuI CTGGAG 2 cut(s) 385, 521
HaeIII GGCC 1 cut(s) 263
HapII CCGG 4 cut(s) 72, 565, 587, 683
HhaI GCGC 1 cut(s) 681
Hin1II CATG 4 cut(s) 174, 410, 679, 798
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 3 cut(s) 194, 314, 711
HpaII CCGG 4 cut(s) 72, 565, 587, 683
HphI GGTGA 2 cut(s) 241, 383
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 4 cut(s) 710, 716, 721, 755
Hpy188III TCNNGA 5 cut(s) 8, 283, 430, 518, 649
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 2 cut(s) 248, 371
HpyAV CCTTC 4 cut(s) 31, 328, 608, 612
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4IV ACGT 2 cut(s) 447, 750
HpyCH4V TGCA 2 cut(s) 220, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 623, 732, 791
HpyF3I CTNAG 3 cut(s) 180, 428, 459
HpySE526I ACGT 2 cut(s) 447, 750
Hsp92II CATG 4 cut(s) 174, 410, 679, 798
HspAI GCGC 1 cut(s) 679
Kzo9I GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 2 cut(s) 221, 669
LweI GCATC 1 cut(s) 781
MaeII ACGT 2 cut(s) 447, 750
MaeIII GTNAC 2 cut(s) 247, 371
MalI GATC 7 cut(s) 471, 479, 569, 653, 695, 718, 777
MboI GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
MboII GAAGA 3 cut(s) 46, 455, 489
MfeI CAATTG 1 cut(s) 376
MhlI GDGCHC 3 cut(s) 458, 465, 706
MluCI AATT 5 cut(s) 123, 376, 410, 434, 620
MlyI GAGTC 2 cut(s) 188, 323
MnlI CCTC 9 cut(s) 37, 50, 71, 152, 290, 350, 437, 637, 684
Mph1103I ATGCAT 1 cut(s) 796
MspA1I CMGCKG 3 cut(s) 391, 541, 735
MspI CCGG 4 cut(s) 72, 565, 587, 683
MspR9I CCNGG 2 cut(s) 587, 683
MunI CAATTG 1 cut(s) 376
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 4 cut(s) 260, 623, 732, 791
NciI CCSGG 2 cut(s) 587, 683
NcoI CCATGG 1 cut(s) 170
NdeII GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
NlaIII CATG 4 cut(s) 174, 410, 679, 798
NmeAIII GCCGAG 1 cut(s) 239
NmuCI GTSAC 2 cut(s) 247, 371
NsiI ATGCAT 1 cut(s) 796
PaeR7I CTCGAG 1 cut(s) 8
PfeI GAWTC 1 cut(s) 711
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 3 cut(s) 236, 393, 659
Ple19I CGATCG 2 cut(s) 472, 778
PleI GAGTC 2 cut(s) 188, 322
PpsI GAGTC 2 cut(s) 188, 322
Psp124BI GAGCTC 2 cut(s) 465, 706
PspFI CCCAGC 1 cut(s) 136
PspPI GGNCC 2 cut(s) 268, 646
PvuI CGATCG 2 cut(s) 472, 778
RsaI GTAC 2 cut(s) 325, 581
RsaNI GTAC 2 cut(s) 324, 580
SacI GAGCTC 2 cut(s) 465, 706
SatI GCNGC 3 cut(s) 235, 392, 658
Sau3AI GATC 7 cut(s) 469, 477, 567, 651, 693, 716, 775
Sau96I GGNCC 2 cut(s) 268, 646
SchI GAGTC 2 cut(s) 188, 323
ScrFI CCNGG 2 cut(s) 587, 683
SduI GDGCHC 3 cut(s) 458, 465, 706
SfaNI GCATC 1 cut(s) 781
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 2 cut(s) 268, 646
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 62, 281
SmoI CTYRAG 3 cut(s) 8, 62, 281
Sse9I AATT 5 cut(s) 123, 376, 410, 434, 620
SsiI CCGC 5 cut(s) 252, 391, 541, 626, 733
SstI GAGCTC 2 cut(s) 465, 706
StyD4I CCNGG 2 cut(s) 585, 681
StyI CCWWGG 2 cut(s) 170, 343
TaaI ACNGT 1 cut(s) 371
TaiI ACGT 2 cut(s) 450, 753
TaqI TCGA 5 cut(s) 9, 80, 519, 636, 774
TaqII GACCGA 1 cut(s) 522
TasI AATT 5 cut(s) 123, 376, 410, 434, 620
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 711
TscAI CASTG 2 cut(s) 184, 463
TseFI GTSAC 2 cut(s) 247, 371
TseI GCWGC 2 cut(s) 234, 657
Tsp45I GTSAC 2 cut(s) 247, 371
TspDTI ATGAA 3 cut(s) 46, 120, 423
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 184, 463
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 268, 646
XapI RAATTY 3 cut(s) 410, 434, 620
XcmI CCANNNNNNNNNTGG 2 cut(s) 262, 341
XhoI CTCGAG 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.