Rh6BG319000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0020590
Physical Location & Seq
Reverse (-)
55048677 .. 55049330
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG319000.1

Sequence Viewer

Length: 654 bp
ATGCACCATTCTCTGGGCTGCCACTTCGTCGTCACCGCCAACTCGGCCGTGGTCCAGCACATCCTCAAGACAAAGTTTCACATCTACCAAAAGGGCGAGTCCTTCCGTACAACTCTCCACGACCTCCTTGGCGATGGGATTTTCAACGCCGACGGTGACAATTGGAAGTTCCAGTGGCAACTCTCCAGCCATGAATTCAACACAAAGTCCCTCAGGAAATTTGTGGAACATGTGGTGGACACTGAGCTCTACGATCGGCTGATCCCCATTCTATCCAAATCTTCCAGCGACCGAATCATTCTCGATTTCCAAGACATTCTCCAGCGGTTCGCCTTCGACAACATTTGCCGGATCGGGTTCGGGTACGACCCGGAGTATCTCCTTCCTTCTCTGCCGCAAGCTAAATTCGCGGTGGCGTTCGACAATGAGGTCCAGATCAGCAGCGAAAGGTTCAGTTCCATGCGCCCGGTGTGGAAGATCAAACGAGCTCTCGGAATCGGATCGGAAAGCCATCTCCGCTGTGTGGTCTCTAACGTCCGAGAGTTTGCGAACACCATCGTCAAGCAAAAGATGCATGAAATGGACGAGAAGAAGACTCTGGAGTCTCATGATCGATCTGCTGTCAAGGTTCTTGAGCACCGGCCATTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

217

Amino Acids

25.21

Weight (kDa)

8.41

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 6 - 201 2.4e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020590)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0268161
rosa_rugosa Rorug06G0039300
rosa_samantha Rh6AG159200 Rh6AG312100 Rh6BG319000 Rh6CG154800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 428, 601
AccB7I CCANNNNNTGG 1 cut(s) 13
AccII CGCG 1 cut(s) 410
AciI CCGC 5 cut(s) 36, 325, 395, 410, 517
AclWI GGATC 3 cut(s) 256, 359, 508
AcoI YGGCCR 2 cut(s) 45, 641
AcsI RAATTY 3 cut(s) 194, 218, 404
AfaI GTAC 2 cut(s) 109, 365
AfiI CCNNNNNNNGG 2 cut(s) 13, 523
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 2 cut(s) 145, 199
AluBI AGCT 3 cut(s) 247, 401, 488
AluI AGCT 3 cut(s) 247, 401, 488
Alw21I GWGCWC 3 cut(s) 249, 490, 639
Alw26I GTCTC 2 cut(s) 532, 609
AlwI GGATC 3 cut(s) 256, 359, 508
AoxI GGCC 2 cut(s) 45, 641
ApeKI GCWGC 2 cut(s) 18, 441
ApoI RAATTY 3 cut(s) 194, 218, 404
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 52, 430
AsuC2I CCSGG 2 cut(s) 371, 467
AsuHPI GGTGA 2 cut(s) 25, 167
AvaII GGWCC 2 cut(s) 52, 430
AxyI CCTNAGG 1 cut(s) 212
BanII GRGCYC 2 cut(s) 249, 490
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 3 cut(s) 249, 490, 639
BbvI GCAGC 2 cut(s) 5, 453
BccI CCATC 3 cut(s) 128, 519, 563
BceAI ACGGC 1 cut(s) 32
BcnI CCSGG 2 cut(s) 371, 467
BcoDI GTCTC 2 cut(s) 532, 609
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 3 cut(s) 19, 395, 442
BlsI GCNGC 3 cut(s) 20, 396, 443
Bme1390I CCNGG 2 cut(s) 371, 467
Bme18I GGWCC 2 cut(s) 52, 430
BmgT120I GGNCC 2 cut(s) 52, 430
BmrFI CCNGG 2 cut(s) 371, 467
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 3 cut(s) 169, 305, 620
BpuEI CTTGAG 2 cut(s) 50, 653
BpuMI CCSGG 2 cut(s) 371, 467
Bsa29I ATCGAT 1 cut(s) 613
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 2 cut(s) 48, 127
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 523
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 1 cut(s) 172
Bse21I CCTNAGG 1 cut(s) 212
BseCI ATCGAT 1 cut(s) 613
BseDI CCNNGG 2 cut(s) 48, 127
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 13, 523
BseMII CTCAG 2 cut(s) 226, 234
BseNI ACTGG 1 cut(s) 172
BseX3I CGGCCG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 5, 453
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 3 cut(s) 48, 256, 292
BshFI GGCC 2 cut(s) 47, 643
BshVI ATCGAT 1 cut(s) 613
BsiEI CGRYCG 3 cut(s) 48, 256, 292
BsiHKAI GWGCWC 3 cut(s) 249, 490, 639
BsiSI CCGG 4 cut(s) 349, 371, 467, 640
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 13, 523
BsmAI GTCTC 2 cut(s) 532, 609
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 2 cut(s) 47, 643
Bso31I GGTCTC 1 cut(s) 532
Bsp1286I GDGCHC 3 cut(s) 249, 490, 639
Bsp143I GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
BspACI CCGC 5 cut(s) 36, 325, 395, 410, 517
BspANI GGCC 2 cut(s) 47, 643
BspCNI CTCAG 2 cut(s) 225, 235
BspDI ATCGAT 1 cut(s) 613
BspFNI CGCG 1 cut(s) 410
BspHI TCATGA 1 cut(s) 607
BspPI GGATC 3 cut(s) 256, 359, 508
BspTNI GGTCTC 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 2 cut(s) 48, 127
BssMI GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
BssT1I CCWWGG 1 cut(s) 127
Bst4CI ACNGT 1 cut(s) 155
BstAPI GCANNNNNTGC 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 212, 243
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 8 cut(s) 256, 264, 354, 438, 480, 503, 613, 617
BstMAI GTCTC 2 cut(s) 532, 609
BstMBI GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
BstMCI CGRYCG 3 cut(s) 48, 256, 292
BstMWI GCNNNNNNNGC 4 cut(s) 44, 407, 516, 571
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 369, 465
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 5, 453
BstV2I GAAGAC 1 cut(s) 599
BstZI CGGCCG 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 613
Bsu36I CCTNAGG 1 cut(s) 212
BsuRI GGCC 2 cut(s) 47, 643
BsuTUI ATCGAT 1 cut(s) 613
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 179, 240
Cac8I GCNNGC 1 cut(s) 399
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 2 cut(s) 52, 430
ClaI ATCGAT 1 cut(s) 613
Csp6I GTAC 2 cut(s) 108, 364
CviAII CATG 5 cut(s) 191, 230, 460, 575, 608
CviJI RGCY 9 cut(s) 18, 47, 189, 247, 259, 401, 488, 510, 643
CviKI_1 RGCY 9 cut(s) 18, 47, 189, 247, 259, 401, 488, 510, 643
CviQI GTAC 2 cut(s) 108, 364
DdeI CTNAG 2 cut(s) 212, 243
DpnI GATC 8 cut(s) 255, 263, 353, 437, 479, 502, 612, 616
DpnII GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
DrdI GACNNNNNNGTC 2 cut(s) 428, 601
DseDI GACNNNNNNGTC 2 cut(s) 428, 601
EaeI YGGCCR 2 cut(s) 45, 641
EagI CGGCCG 1 cut(s) 45
Ecl136II GAGCTC 2 cut(s) 247, 488
EclXI CGGCCG 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 249, 490
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 52, 430
Eco52I CGGCCG 1 cut(s) 45
Eco53kI GAGCTC 2 cut(s) 247, 488
Eco81I CCTNAGG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 247, 488
EcoRI GAATTC 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 576
EcoT38I GRGCYC 2 cut(s) 249, 490
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 5 cut(s) 194, 233, 463, 578, 611
FaiI YATR 5 cut(s) 192, 231, 461, 576, 609
FaqI GGGAC 1 cut(s) 193
FatI CATG 5 cut(s) 190, 229, 459, 574, 607
Fnu4HI GCNGC 3 cut(s) 19, 395, 442
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 2 cut(s) 249, 490
Fsp4HI GCNGC 3 cut(s) 19, 395, 442
GlaI GCGC 1 cut(s) 464
GluI GCNGC 3 cut(s) 19, 395, 442
GsuI CTGGAG 3 cut(s) 169, 305, 620
HaeIII GGCC 2 cut(s) 47, 643
HapII CCGG 4 cut(s) 349, 371, 467, 640
HhaI GCGC 1 cut(s) 465
Hin1II CATG 5 cut(s) 194, 233, 463, 578, 611
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HinfI GANTC 5 cut(s) 98, 294, 495, 595, 602
HpaII CCGG 4 cut(s) 349, 371, 467, 640
HphI GGTGA 2 cut(s) 25, 167
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 5 cut(s) 494, 500, 505, 539, 650
Hpy188III TCNNGA 7 cut(s) 67, 214, 302, 433, 599, 608, 632
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 2 cut(s) 32, 155
HpyAV CCTTC 4 cut(s) 112, 343, 392, 396
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 2 cut(s) 4, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 407, 516, 571
HpyF3I CTNAG 2 cut(s) 212, 243
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 5 cut(s) 194, 233, 463, 578, 611
HspAI GCGC 1 cut(s) 463
Kzo9I GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
Lsp1109I GCAGC 2 cut(s) 5, 453
LweI GCATC 1 cut(s) 561
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 2 cut(s) 31, 155
MalI GATC 8 cut(s) 255, 263, 353, 437, 479, 502, 612, 616
MboI GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
MboII GAAGA 4 cut(s) 273, 487, 601, 604
MfeI CAATTG 1 cut(s) 160
MhlI GDGCHC 3 cut(s) 249, 490, 639
MluCI AATT 4 cut(s) 160, 194, 218, 404
MlyI GAGTC 3 cut(s) 107, 589, 611
MnlI CCTC 4 cut(s) 74, 134, 221, 421
Mph1103I ATGCAT 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 649
MspA1I CMGCKG 2 cut(s) 325, 519
MspI CCGG 4 cut(s) 349, 371, 467, 640
MspR9I CCNGG 2 cut(s) 371, 467
MunI CAATTG 1 cut(s) 160
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 4 cut(s) 44, 407, 516, 571
NciI CCSGG 2 cut(s) 371, 467
NdeII GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
NlaIII CATG 5 cut(s) 194, 233, 463, 578, 611
NmeAIII GCCGAG 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 31, 155
NsiI ATGCAT 1 cut(s) 576
NspI RCATGY 1 cut(s) 233
PagI TCATGA 1 cut(s) 607
PciI ACATGT 1 cut(s) 229
PfeI GAWTC 2 cut(s) 294, 495
PflMI CCANNNNNTGG 1 cut(s) 13
PkrI GCNGC 3 cut(s) 20, 396, 443
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 3 cut(s) 106, 589, 610
PpsI GAGTC 3 cut(s) 106, 589, 610
PscI ACATGT 1 cut(s) 229
Psp124BI GAGCTC 2 cut(s) 249, 490
PspPI GGNCC 2 cut(s) 52, 430
PvuI CGATCG 1 cut(s) 256
RsaI GTAC 2 cut(s) 109, 365
RsaNI GTAC 2 cut(s) 108, 364
RseI CAYNNNNRTG 1 cut(s) 649
SacI GAGCTC 2 cut(s) 249, 490
SatI GCNGC 3 cut(s) 19, 395, 442
Sau3AI GATC 8 cut(s) 253, 261, 351, 435, 477, 500, 610, 614
Sau96I GGNCC 2 cut(s) 52, 430
SchI GAGTC 3 cut(s) 107, 589, 611
ScrFI CCNGG 2 cut(s) 371, 467
SduI GDGCHC 3 cut(s) 249, 490, 639
SetI ASST 8 cut(s) 126, 249, 403, 432, 452, 490, 537, 630
SfaNI GCATC 1 cut(s) 561
SinI GGWCC 2 cut(s) 52, 430
SmiMI CAYNNNNRTG 1 cut(s) 649
SmlI CTYRAG 2 cut(s) 65, 632
SmoI CTYRAG 2 cut(s) 65, 632
Sse9I AATT 4 cut(s) 160, 194, 218, 404
SsiI CCGC 5 cut(s) 36, 325, 395, 410, 517
SstI GAGCTC 2 cut(s) 249, 490
StyD4I CCNGG 2 cut(s) 369, 465
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 537
TaqI TCGA 4 cut(s) 303, 336, 420, 613
TaqII GACCGA 1 cut(s) 306
TasI AATT 4 cut(s) 160, 194, 218, 404
TauI GCSGC 1 cut(s) 397
TfiI GAWTC 2 cut(s) 294, 495
TscAI CASTG 2 cut(s) 179, 247
TseFI GTSAC 2 cut(s) 31, 155
TseI GCWGC 2 cut(s) 18, 441
Tsp45I GTSAC 2 cut(s) 31, 155
TspDTI ATGAA 2 cut(s) 207, 591
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 2 cut(s) 179, 247
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 52, 430
XapI RAATTY 3 cut(s) 194, 218, 404
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 125
Zsp2I ATGCAT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.