Rh6BG125500

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007673
Physical Location & Seq
Reverse (-)
19544894 .. 19549469
4576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG125500.1

Sequence Viewer

Length: 1119 bp
ATGGCATCCCTCACCTCACAACTCCCAATCCTCCTCTTCCTCTTTGTCACCGCTTTCACTTCCGCAGACTCCCAGTCGTTCATCGGCGTCAACTACGGCCAGGTTGCAGACAACCTCCCGGCGCCGGCCGCCACCGCCAAGCTCCTCCAGTCCACCGCCATCAAGCAAGTCCGCCTCTACGGATCCGACCCCGACGTCATCAAGGCCCTCGCCAACACCGGTATCGGCATCGTCATCGGAATCGCCAACGGCGCCATCCCCTCCCTCGCCGCCGATCCCAACGCCGCCGCCCAGTGGGTCAACTCGAACGTCGTCCCGTTCTACCCGGCCAGCAATATCACCCTCATCAACGTCGGCAATGAGGTCCTCTTCTCCAACGACCAGGGCCTCATCTCGCAGCTCCTCCCCGCCATGCGAAATGTCCAGTCTGCCCTCGCCGCCGCCTCCCTCGGCGGGAAGGTCCGCATCTCCTCCGTCCACGCCATGACGTTGCTGTCGCAGTCCGACCCGCCCTCCTCGGGTCGGTTCAACCCGGCGTTGGTTGACGTCCTCAAGGGGCTGCTGGCGTTCCAGAGGGACAATGCCTCGCCCTTCGCCATCAACCCGTACCCGTTTTTCGCCTACCAGAGCGACCCGAGACCCGAAACGCTGGCGTTTTGCCTCTTCCAGCCCAACGCGGGACGAGTCGACCCGGGCTCCGGGATCAAGTACATGAACATGTTCGATGCTCAGGTTGATGCTGTGAAGTCTGCCCTGAATGCAATTGGATTCAATGACATTGATATTTTGGTGGCCGAGACAGGATGGCCATACCGCGGAGATAGCAATGAAGTTGGACCTAGTGTTGAGAATGCAAAGGCATACAATGGCAATTTGATTCAGCATCTTAGATCCATGGTTGGGACTCCATTGATTCCTGGAAAACCTGTGGAGACATACATATTTGCACTCTATGATGAGGATCTGAAACCTGGTCCAACCTCTGAGCGATCATTCGGGCTGTTCAAGCTTGATTTATCTCCCACTTATGATGCTGGTCTCTCAAAGAGTAGCCAGGTAAACACAATATATAACCTCATTACCCTTTATAGGACTTATCTGGTTCACATGGAAAGCTAA

Protein Analysis

372

Amino Acids

39.78

Weight (kDa)

5.09

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 28 - 344 5e-76 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 194, 493
AatII GACGTC 2 cut(s) 198, 549
AccB1I GGYRCC 2 cut(s) 121, 251
AccI GTMKAC 1 cut(s) 687
AccII CGCG 2 cut(s) 677, 816
AclWI GGATC 6 cut(s) 177, 190, 269, 710, 885, 969
AcoI YGGCCR 5 cut(s) 97, 126, 327, 792, 806
AcyI GRCGYC 5 cut(s) 87, 122, 195, 252, 546
AfaI GTAC 2 cut(s) 608, 710
AflIII ACRYGT 1 cut(s) 717
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 529, 772, 1006
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 5 cut(s) 99, 381, 916, 970, 1053
AluBI AGCT 4 cut(s) 142, 400, 1009, 1116
AluI AGCT 4 cut(s) 142, 400, 1009, 1116
Alw26I GTCTC 4 cut(s) 631, 791, 926, 1043
AlwI GGATC 6 cut(s) 177, 190, 269, 710, 885, 969
Ama87I CYCGRG 3 cut(s) 517, 634, 691
AoxI GGCC 7 cut(s) 97, 126, 204, 327, 385, 792, 806
ApeKI GCWGC 2 cut(s) 397, 559
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 719
AspLEI GCGC 2 cut(s) 124, 254
AspS9I GGNCC 6 cut(s) 205, 364, 385, 460, 836, 974
AsuC2I CCSGG 6 cut(s) 119, 326, 533, 692, 693, 700
AsuHPI GGTGA 3 cut(s) 4, 40, 331
AvaI CYCGRG 3 cut(s) 517, 634, 691
AvaII GGWCC 4 cut(s) 364, 460, 836, 974
BaeI ACNNNNGTAYC 2 cut(s) 205, 238
BalI TGGCCA 1 cut(s) 808
BamHI GGATCC 1 cut(s) 182
BanI GGYRCC 2 cut(s) 121, 251
BanII GRGCYC 1 cut(s) 698
BbvI GCAGC 2 cut(s) 409, 546
BccI CCATC 4 cut(s) 167, 263, 605, 798
BceAI ACGGC 2 cut(s) 112, 265
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 5 cut(s) 101, 383, 918, 972, 1055
BcnI CCSGG 6 cut(s) 119, 326, 533, 692, 693, 700
BcoDI GTCTC 4 cut(s) 631, 791, 926, 1043
BfaI CTAG 1 cut(s) 840
BfoI RGCGCY 2 cut(s) 125, 255
BisI GCNGC 8 cut(s) 129, 270, 285, 288, 398, 438, 441, 560
BlsI GCNGC 8 cut(s) 130, 271, 286, 289, 399, 439, 442, 561
Bme18I GGWCC 4 cut(s) 364, 460, 836, 974
BmeRI GACNNNNNGTC 1 cut(s) 73
BmeT110I CYCGRG 3 cut(s) 517, 634, 691
BmgT120I GGNCC 6 cut(s) 205, 364, 385, 460, 836, 974
BmiI GGNNCC 4 cut(s) 123, 184, 253, 697
BmrI ACTGGG 2 cut(s) 67, 286
BmsI GCATC 7 cut(s) 14, 237, 474, 715, 727, 892, 1021
BmuI ACTGGG 2 cut(s) 67, 286
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 729
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 6 cut(s) 119, 326, 533, 692, 693, 700
BsaBI GATNNNNATC 1 cut(s) 960
BsaHI GRCGYC 5 cut(s) 87, 122, 195, 252, 546
BsaI GGTCTC 2 cut(s) 631, 1043
BsaJI CCNNGG 6 cut(s) 382, 448, 516, 691, 814, 894
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 680, 710
Bse118I RCCGGY 2 cut(s) 124, 218
Bse1I ACTGG 4 cut(s) 73, 148, 292, 424
Bse3DI GCAATG 2 cut(s) 364, 832
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 5 cut(s) 101, 383, 918, 972, 1055
BseDI CCNNGG 6 cut(s) 382, 448, 516, 691, 814, 894
BseGI GGATG 3 cut(s) 5, 255, 809
BseJI GATNNNNATC 1 cut(s) 960
BseMI GCAATG 2 cut(s) 364, 832
BseMII CTCAG 2 cut(s) 743, 975
BseNI ACTGG 4 cut(s) 73, 148, 292, 424
BseRI GAGGAG 5 cut(s) 23, 134, 392, 460, 505
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 409, 546
Bsh1236I CGCG 2 cut(s) 677, 816
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 7 cut(s) 99, 128, 206, 329, 387, 794, 808
BshNI GGYRCC 2 cut(s) 121, 251
BshTI ACCGGT 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 129
BsiHKCI CYCGRG 3 cut(s) 517, 634, 691
BsiSI CCGG 7 cut(s) 119, 125, 219, 326, 533, 692, 699
BslFI GGGAC 4 cut(s) 299, 590, 693, 916
BsmAI GTCTC 4 cut(s) 631, 791, 926, 1043
BsmFI GGGAC 4 cut(s) 299, 590, 693, 916
BsmI GAATGC 2 cut(s) 763, 856
BsnI GGCC 7 cut(s) 99, 128, 206, 329, 387, 794, 808
Bso31I GGTCTC 2 cut(s) 631, 1043
BsoBI CYCGRG 3 cut(s) 517, 634, 691
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 6 cut(s) 182, 274, 702, 890, 961, 989
Bsp19I CCATGG 1 cut(s) 894
BspANI GGCC 7 cut(s) 99, 128, 206, 329, 387, 794, 808
BspCNI CTCAG 2 cut(s) 742, 976
BspFNI CGCG 2 cut(s) 677, 816
BspLI GGNNCC 4 cut(s) 123, 184, 253, 697
BspPI GGATC 6 cut(s) 177, 190, 269, 710, 885, 969
BspT107I GGYRCC 2 cut(s) 121, 251
BspTNI GGTCTC 2 cut(s) 631, 1043
BsrDI GCAATG 2 cut(s) 364, 832
BsrFI RCCGGY 2 cut(s) 124, 218
BsrI ACTGG 4 cut(s) 73, 148, 292, 424
BssAI RCCGGY 2 cut(s) 124, 218
BssECI CCNNGG 6 cut(s) 382, 448, 516, 691, 814, 894
BssMI GATC 6 cut(s) 182, 274, 702, 890, 961, 989
BssNI GRCGYC 5 cut(s) 87, 122, 195, 252, 546
BssT1I CCWWGG 1 cut(s) 894
Bst2UI CCWGG 5 cut(s) 101, 383, 918, 972, 1055
Bst6I CTCTTC 3 cut(s) 41, 374, 668
BstACI GRCGYC 5 cut(s) 87, 122, 195, 252, 546
BstC8I GCNNGC 4 cut(s) 126, 331, 564, 651
BstDEI CTNAG 3 cut(s) 729, 887, 984
BstDSI CCRYGG 2 cut(s) 814, 894
BstF5I GGATG 3 cut(s) 5, 255, 809
BstFNI CGCG 2 cut(s) 677, 816
BstH2I RGCGCY 2 cut(s) 125, 255
BstHHI GCGC 2 cut(s) 124, 254
BstKTI GATC 6 cut(s) 185, 277, 705, 893, 964, 992
BstMAI GTCTC 4 cut(s) 631, 791, 926, 1043
BstMBI GATC 6 cut(s) 182, 274, 702, 890, 961, 989
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 7 cut(s) 128, 134, 251, 437, 758, 822, 1006
BstNI CCWGG 5 cut(s) 101, 383, 918, 972, 1055
BstNSI RCATGY 1 cut(s) 721
BstUI CGCG 2 cut(s) 677, 816
BstV1I GCAGC 2 cut(s) 409, 546
BstX2I RGATCY 3 cut(s) 182, 890, 961
BstYI RGATCY 3 cut(s) 182, 890, 961
BstZI CGGCCG 1 cut(s) 126
BsuRI GGCC 7 cut(s) 99, 128, 206, 329, 387, 794, 808
BtgI CCRYGG 2 cut(s) 814, 894
BtsCI GGATG 3 cut(s) 5, 255, 809
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 4 cut(s) 126, 331, 564, 651
CfoI GCGC 2 cut(s) 124, 254
Cfr10I RCCGGY 2 cut(s) 124, 218
Cfr13I GGNCC 6 cut(s) 205, 364, 385, 460, 836, 974
Cfr42I CCGCGG 1 cut(s) 817
Cfr9I CCCGGG 1 cut(s) 691
CseI GACGC 1 cut(s) 76
CsiI ACCWGGT 1 cut(s) 970
Csp6I GTAC 2 cut(s) 607, 709
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 6 cut(s) 412, 484, 712, 718, 895, 1108
CviQI GTAC 2 cut(s) 607, 709
DdeI CTNAG 3 cut(s) 729, 887, 984
DinI GGCGCC 2 cut(s) 123, 253
DpnI GATC 6 cut(s) 184, 276, 704, 892, 963, 991
DpnII GATC 6 cut(s) 182, 274, 702, 890, 961, 989
DrdI GACNNNNNNGTC 2 cut(s) 194, 493
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 2 cut(s) 194, 493
EaeI YGGCCR 5 cut(s) 97, 126, 327, 792, 806
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 41, 374, 668
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 3 cut(s) 41, 374, 668
EciI GGCGGA 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 894
Eco24I GRGCYC 1 cut(s) 698
Eco31I GGTCTC 2 cut(s) 631, 1043
Eco47I GGWCC 4 cut(s) 364, 460, 836, 974
Eco52I CGGCCG 1 cut(s) 126
Eco88I CYCGRG 3 cut(s) 517, 634, 691
EcoO109I RGGNCCY 3 cut(s) 205, 364, 385
EcoRII CCWGG 5 cut(s) 99, 381, 916, 970, 1053
EcoT14I CCWWGG 1 cut(s) 894
EcoT38I GRGCYC 1 cut(s) 698
EgeI GGCGCC 2 cut(s) 123, 253
EheI GGCGCC 2 cut(s) 123, 253
ErhI CCWWGG 1 cut(s) 894
FaeI CATG 6 cut(s) 415, 487, 715, 721, 898, 1111
FaqI GGGAC 4 cut(s) 299, 590, 693, 916
FatI CATG 6 cut(s) 411, 483, 711, 717, 894, 1107
FauI CCCGC 4 cut(s) 415, 446, 516, 670
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 8 cut(s) 129, 270, 285, 288, 398, 438, 441, 560
FokI GGATG 2 cut(s) 242, 816
FriOI GRGCYC 1 cut(s) 698
Fsp4HI GCNGC 8 cut(s) 129, 270, 285, 288, 398, 438, 441, 560
FspBI CTAG 1 cut(s) 840
GlaI GCGC 2 cut(s) 123, 253
GluI GCNGC 8 cut(s) 129, 270, 285, 288, 398, 438, 441, 560
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 2 cut(s) 125, 255
HaeIII GGCC 7 cut(s) 99, 128, 206, 329, 387, 794, 808
HapII CCGG 7 cut(s) 119, 125, 219, 326, 533, 692, 699
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 124, 254
Hin1I GRCGYC 5 cut(s) 87, 122, 195, 252, 546
Hin1II CATG 6 cut(s) 415, 487, 715, 721, 898, 1111
Hin6I GCGC 2 cut(s) 122, 252
HinP1I GCGC 2 cut(s) 122, 252
HincII GTYRAC 4 cut(s) 91, 301, 544, 688
HindII GTYRAC 4 cut(s) 91, 301, 544, 688
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 7 cut(s) 68, 240, 684, 768, 877, 904, 913
HpaII CCGG 7 cut(s) 119, 125, 219, 326, 533, 692, 699
HphI GGTGA 3 cut(s) 4, 40, 331
Hpy166II GTNNAC 8 cut(s) 91, 153, 301, 478, 544, 688, 1060, 1105
Hpy188I TCNGA 5 cut(s) 187, 239, 505, 966, 985
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 8 cut(s) 91, 153, 301, 478, 544, 688, 1060, 1105
Hpy99I CGWCG 3 cut(s) 197, 314, 356
HpyAV CCTTC 2 cut(s) 451, 601
HpyCH4IV ACGT 5 cut(s) 195, 309, 351, 488, 546
HpyCH4V TGCA 4 cut(s) 107, 761, 854, 947
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 134, 251, 437, 758, 822, 1006
HpyF3I CTNAG 3 cut(s) 729, 887, 984
HpySE526I ACGT 5 cut(s) 195, 309, 351, 488, 546
Hsp92I GRCGYC 5 cut(s) 87, 122, 195, 252, 546
Hsp92II CATG 6 cut(s) 415, 487, 715, 721, 898, 1111
HspAI GCGC 2 cut(s) 122, 252
KasI GGCGCC 2 cut(s) 121, 251
KroI GCCGGC 1 cut(s) 124
KroNI GCCGGC 1 cut(s) 126
KspI CCGCGG 1 cut(s) 817
Kzo9I GATC 6 cut(s) 182, 274, 702, 890, 961, 989
LmnI GCTCC 3 cut(s) 147, 405, 701
Lsp1109I GCAGC 2 cut(s) 409, 546
LweI GCATC 7 cut(s) 14, 237, 474, 715, 727, 892, 1021
MabI ACCWGGT 1 cut(s) 970
MaeI CTAG 1 cut(s) 840
MaeII ACGT 5 cut(s) 195, 309, 351, 488, 546
MaeIII GTNAC 1 cut(s) 46
MalI GATC 6 cut(s) 184, 276, 704, 892, 963, 991
MboI GATC 6 cut(s) 182, 274, 702, 890, 961, 989
MboII GAAGA 3 cut(s) 28, 361, 655
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 182, 890, 961
MhlI GDGCHC 1 cut(s) 698
MlsI TGGCCA 1 cut(s) 808
MluCI AATT 2 cut(s) 762, 871
MluNI TGGCCA 1 cut(s) 808
Mly113I GGCGCC 2 cut(s) 122, 252
MlyI GAGTC 3 cut(s) 62, 693, 898
MmeI TCCRAC 5 cut(s) 210, 399, 528, 814, 1001
Mox20I TGGCCA 1 cut(s) 808
MroNI GCCGGC 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 1 cut(s) 808
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 808
MspA1I CMGCKG 1 cut(s) 816
MspI CCGG 7 cut(s) 119, 125, 219, 326, 533, 692, 699
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 2 cut(s) 763, 856
MvaI CCWGG 5 cut(s) 101, 383, 918, 972, 1055
MvnI CGCG 2 cut(s) 677, 816
MwoI GCNNNNNNNGC 7 cut(s) 128, 134, 251, 437, 758, 822, 1006
NaeI GCCGGC 1 cut(s) 126
NarI GGCGCC 2 cut(s) 122, 252
NciI CCSGG 6 cut(s) 119, 326, 533, 692, 693, 700
NcoI CCATGG 1 cut(s) 894
NdeII GATC 6 cut(s) 182, 274, 702, 890, 961, 989
NgoMIV GCCGGC 1 cut(s) 124
NlaIII CATG 6 cut(s) 415, 487, 715, 721, 898, 1111
NlaIV GGNNCC 4 cut(s) 123, 184, 253, 697
NmeAIII GCCGAG 2 cut(s) 429, 820
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 721
PciI ACATGT 1 cut(s) 717
PctI GAATGC 2 cut(s) 763, 856
PdiI GCCGGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 4 cut(s) 240, 768, 877, 913
PfoI TCCNGGA 2 cut(s) 698, 916
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 8 cut(s) 130, 271, 286, 289, 399, 439, 442, 561
PleI GAGTC 3 cut(s) 62, 692, 898
PluTI GGCGCC 2 cut(s) 125, 255
PpsI GAGTC 3 cut(s) 62, 692, 898
PpuMI RGGWCCY 1 cut(s) 364
PscI ACATGT 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 5 cut(s) 99, 381, 916, 970, 1053
PspGI CCWGG 5 cut(s) 99, 381, 916, 970, 1053
PspN4I GGNNCC 4 cut(s) 123, 184, 253, 697
PspPI GGNCC 6 cut(s) 205, 364, 385, 460, 836, 974
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 3 cut(s) 182, 890, 961
RsaI GTAC 2 cut(s) 608, 710
RsaNI GTAC 2 cut(s) 607, 709
RseI CAYNNNNRTG 1 cut(s) 716
SacII CCGCGG 1 cut(s) 817
SalI GTCGAC 1 cut(s) 686
SatI GCNGC 8 cut(s) 129, 270, 285, 288, 398, 438, 441, 560
Sau3AI GATC 6 cut(s) 182, 274, 702, 890, 961, 989
Sau96I GGNCC 6 cut(s) 205, 364, 385, 460, 836, 974
SchI GAGTC 3 cut(s) 62, 693, 898
SduI GDGCHC 1 cut(s) 698
SexAI ACCWGGT 1 cut(s) 970
SfaNI GCATC 7 cut(s) 14, 237, 474, 715, 727, 892, 1021
SfoI GGCGCC 2 cut(s) 123, 253
Sfr303I CCGCGG 1 cut(s) 817
SgrBI CCGCGG 1 cut(s) 817
SinI GGWCC 4 cut(s) 364, 460, 836, 974
SmaI CCCGGG 1 cut(s) 693
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 2 cut(s) 762, 871
SspDI GGCGCC 2 cut(s) 121, 251
SspMI CTAG 1 cut(s) 840
StyI CCWWGG 1 cut(s) 894
TaiI ACGT 5 cut(s) 198, 312, 354, 491, 549
TaqI TCGA 3 cut(s) 305, 687, 723
TasI AATT 2 cut(s) 762, 871
TatI WGTACW 1 cut(s) 708
TauI GCSGC 6 cut(s) 131, 272, 287, 290, 440, 443
TfiI GAWTC 4 cut(s) 240, 768, 877, 913
TscAI CASTG 1 cut(s) 299
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 397, 559
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 3 cut(s) 70, 728, 843
TspGWI ACGGA 2 cut(s) 195, 463
TspMI CCCGGG 1 cut(s) 691
TspRI CASTG 1 cut(s) 299
VpaK11BI GGWCC 4 cut(s) 364, 460, 836, 974
XceI RCATGY 1 cut(s) 721
XmaI CCCGGG 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 1 cut(s) 840
ZraI GACGTC 2 cut(s) 196, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.