Rh6BG153600
BHLH Family

Transcription factor bHLH3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0019319
Physical Location & Seq
Forward (+)
24895779 .. 24896225
447 bp
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UTR
Exon/CDS
Intron
Rh6BG153600.1

Sequence Viewer

Length: 447 bp
ATGGGTGAGAAGCTTTGGGTGAATGAGGAAGAAAAGGTCATAGTGGAGTCAGTACCAGGCACCGAAGCGTGCCAGGTGTTGATCTCATTGGCTTTGGAGAACTTGTTGTCCGTCTTGGCTAGGCCTCCGGGACAATTGGGAGTGCAGCTGGGGCTGTCCCAGCTTGTCCAAGGGTCCAGTTGGGATTACGCTGCCTTCTGGCAGGTTGTGGGATTGAAATCTGGTGGAGCTGCCTTGATTTGGGGTGATGGACATTGCAGAGACACCAAGGGTGGTGGGGTTGGAGACGAGAGTTCTAGTAGAGATAGCAACTTGGAGGGAGTGCAGAAGAAGAATGCGGAGGTGAGTGGGTGCTTGGGAAGCTCCACTCATGTTTCGGTGGTTCGGGTGAGGATAACTATGCGAGGAGGTTGGATCGGTTGTCAGATGTGGAAATGTTTTACCTGA

Protein Analysis

148

Amino Acids

15.78

Weight (kDa)

5.67

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 50 - 115 9.7e-12 bHLH-MYC and R2R3-MYB transcription factors N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019319)

Species Orthologous Gene IDs
pyrus_communis pycom05g18910
rosa_chinensis RchiOBHm_Chr6g0268101
rosa_samantha Rh6AG158800 Rh6BG153600 Rh6BG163000 Rh6CG155500 Rh6DG138400 Rh6DG148800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 59
AciI CCGC 1 cut(s) 338
AclWI GGATC 1 cut(s) 422
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 2 cut(s) 55, 72
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 5 cut(s) 13, 148, 163, 230, 363
AluI AGCT 5 cut(s) 13, 148, 163, 230, 363
Alw26I GTCTC 2 cut(s) 255, 279
AlwI GGATC 1 cut(s) 422
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 3 cut(s) 145, 191, 230
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 5 cut(s) 17, 31, 257, 355, 400
AvaII GGWCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 157, 178, 217
BccI CCATC 1 cut(s) 242
BciT130I CCWGG 2 cut(s) 57, 74
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 255, 279
BfaI CTAG 2 cut(s) 120, 297
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 3 cut(s) 146, 192, 231
BlsI GCNGC 3 cut(s) 147, 193, 232
Bme1390I CCNGG 3 cut(s) 57, 74, 129
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 61, 175
BmrFI CCNGG 3 cut(s) 57, 74, 129
BpuMI CCSGG 1 cut(s) 129
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 169, 267
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 253
Bse8I GATNNNNATC 1 cut(s) 217
BseBI CCWGG 2 cut(s) 57, 74
BseDI CCNNGG 2 cut(s) 169, 267
BseJI GATNNNNATC 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMI GCAATG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 3 cut(s) 157, 178, 217
BseYI CCCAGC 2 cut(s) 148, 159
BsgI GTGCAG 2 cut(s) 164, 344
BshFI GGCC 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 142, 144
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 2 cut(s) 255, 279
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 2 cut(s) 142, 144
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 1 cut(s) 124
Bsp143I GATC 2 cut(s) 81, 414
BspACI CCGC 1 cut(s) 338
BspANI GGCC 1 cut(s) 124
BspLI GGNNCC 2 cut(s) 61, 175
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 253
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 169, 267
BssMI GATC 2 cut(s) 81, 414
BssT1I CCWWGG 2 cut(s) 169, 267
Bst2UI CCWGG 2 cut(s) 57, 74
BstC8I GCNNGC 1 cut(s) 70
BstKTI GATC 2 cut(s) 84, 417
BstMAI GTCTC 2 cut(s) 255, 279
BstMBI GATC 2 cut(s) 81, 414
BstMWI GCNNNNNNNGC 3 cut(s) 151, 160, 360
BstNI CCWGG 2 cut(s) 57, 74
BstSCI CCNGG 3 cut(s) 55, 72, 127
BstV1I GCAGC 3 cut(s) 157, 178, 217
BsuRI GGCC 1 cut(s) 124
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 53
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviAII CATG 1 cut(s) 371
CviJI RGCY 9 cut(s) 13, 92, 119, 124, 148, 154, 163, 230, 363
CviKI_1 RGCY 9 cut(s) 13, 92, 119, 124, 148, 154, 163, 230, 363
CviQI GTAC 1 cut(s) 53
DpnI GATC 2 cut(s) 83, 416
DpnII GATC 2 cut(s) 81, 414
Eco130I CCWWGG 2 cut(s) 169, 267
Eco147I AGGCCT 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 174
EcoRII CCWGG 2 cut(s) 55, 72
EcoT14I CCWWGG 2 cut(s) 169, 267
ErhI CCWWGG 2 cut(s) 169, 267
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 1 cut(s) 374
FaiI YATR 3 cut(s) 41, 372, 401
FaqI GGGAC 2 cut(s) 142, 144
FatI CATG 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 146, 192, 231
Fsp4HI GCNGC 3 cut(s) 146, 192, 231
FspBI CTAG 2 cut(s) 120, 297
GluI GCNGC 3 cut(s) 146, 192, 231
GsaI CCCAGC 2 cut(s) 152, 163
HaeIII GGCC 1 cut(s) 124
HapII CCGG 1 cut(s) 128
Hin1II CATG 1 cut(s) 374
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 1 cut(s) 47
HpaII CCGG 1 cut(s) 128
HphI GGTGA 5 cut(s) 17, 31, 257, 355, 400
Hpy188I TCNGA 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 145, 258, 325
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 160, 360
Hsp92II CATG 1 cut(s) 374
Kzo9I GATC 2 cut(s) 81, 414
LmnI GCTCC 2 cut(s) 227, 368
Lsp1109I GCAGC 3 cut(s) 157, 178, 217
MaeI CTAG 2 cut(s) 120, 297
MalI GATC 2 cut(s) 83, 416
MboI GATC 2 cut(s) 81, 414
MboII GAAGA 3 cut(s) 41, 340, 343
MfeI CAATTG 1 cut(s) 134
MluCI AATT 1 cut(s) 134
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 262, 392
MnlI CCTC 7 cut(s) 19, 135, 310, 334, 384, 398, 401
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 3 cut(s) 57, 74, 129
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 340
MvaI CCWGG 2 cut(s) 57, 74
MwoI GCNNNNNNNGC 3 cut(s) 151, 160, 360
NciI CCSGG 1 cut(s) 129
NdeII GATC 2 cut(s) 81, 414
NlaIII CATG 1 cut(s) 374
NlaIV GGNNCC 2 cut(s) 61, 175
PceI AGGCCT 1 cut(s) 124
PctI GAATGC 1 cut(s) 340
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 3 cut(s) 147, 193, 232
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 55, 72
PspFI CCCAGC 2 cut(s) 148, 159
PspGI CCWGG 2 cut(s) 55, 72
PspN4I GGNNCC 2 cut(s) 61, 175
PspPI GGNCC 1 cut(s) 174
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SatI GCNGC 3 cut(s) 146, 192, 231
Sau3AI GATC 2 cut(s) 81, 414
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 57, 74, 129
SinI GGWCC 1 cut(s) 174
Sse9I AATT 1 cut(s) 134
SseBI AGGCCT 1 cut(s) 124
SsiI CCGC 1 cut(s) 338
SspMI CTAG 2 cut(s) 120, 297
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 3 cut(s) 55, 72, 127
StyI CCWWGG 2 cut(s) 169, 267
TasI AATT 1 cut(s) 134
TseI GCWGC 3 cut(s) 145, 191, 230
TspGWI ACGGA 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 174
XspI CTAG 2 cut(s) 120, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.