Rh6BG251900

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017242
Physical Location & Seq
Forward (+)
46321569 .. 46322030
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG251900.1

Sequence Viewer

Length: 462 bp
ATGGCTCCCCAGTTCATGTTTCTCATTGGCAAACCTCCCGTTGTCATCATTCTTGTTCCTTTCTTGTGTATCTTAGCCTATGTCATTGCCATGTTCACTCGGCCAATTCTATGGTTTGCACGGTTACTACTTAACCGCCGAGGCGTCGAGGACAGGATTCTCCGGTCATTTCCAAAGATCTGTTACACTGCAGAAGTCGATGAAAAGATCACAGAATGCGCTGTCTGCTTGATGGAGTATGAGGATGGAGATGCATTAAGGATTTTGCCTCCTTGTGGTCATGGTTTCCATGTTGGCTGTGTTGATAGGTGGCTGAAGTGCCACTCTTCATGTCCATCGTGTCGCGCCGTTGTAGTTATGGAGAGCAAAGCACAGATACTTGGAGACGCCAATAGTCCACCATTCTCAGATACATCGTATACAGTTTCAAATCCGTACAGACTGACTTTCTGGCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

153

Amino Acids

17.37

Weight (kDa)

7.54

Isoelectric Point (pI)

60.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 72 - 115 1.7e-14 Ring finger domain
zf-RING_11 PF17123 72 - 101 1e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 72 - 114 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 73 - 115 8.4e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 73 - 114 1.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017242)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g16840
rosa_chinensis RchiOBHm_Chr6g0280971
rosa_laevigata RLG00000013011
rosa_multiflora Rmu_co8316755.1_g000001 Rmu_sc0030247.1_g000001 Rmu_sc0030424.1_g000002
rosa_roxburghii Rroxscaffold_7G00187440
rosa_rugosa Rorug06G0137700
rosa_samantha Rh6AG249700 Rh6BG251900 Rh6CG252400 Rh6DG244200
rosa_wichuraiana Rw6G021610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 419
AccII CGCG 1 cut(s) 345
AciI CCGC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 2 cut(s) 144, 387
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 378
AoxI GGCC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 221, 347
BccI CCATC 3 cut(s) 226, 239, 343
BceAI ACGGC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 189
BglII AGATCT 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 4
BsaHI GRCGYC 2 cut(s) 144, 387
BsaJI CCNNGG 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 378
BsmBI CGTCTC 1 cut(s) 378
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 1 cut(s) 103
Bsp143I GATC 2 cut(s) 177, 207
BspACI CCGC 1 cut(s) 136
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 419
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 177, 207
BssNAI GTATAC 1 cut(s) 420
BssNI GRCGYC 2 cut(s) 144, 387
Bst1107I GTATAC 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 123, 424
Bst6I CTCTTC 1 cut(s) 331
BstACI GRCGYC 2 cut(s) 144, 387
BstDEI CTNAG 2 cut(s) 73, 406
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 2 cut(s) 221, 347
BstKTI GATC 2 cut(s) 180, 210
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 2 cut(s) 177, 207
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 1 cut(s) 177
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 186
CfoI GCGC 2 cut(s) 221, 347
CseI GACGC 2 cut(s) 133, 395
Csp6I GTAC 1 cut(s) 436
CviAII CATG 5 cut(s) 16, 91, 281, 290, 330
CviJI RGCY 5 cut(s) 5, 77, 103, 297, 313
CviKI_1 RGCY 5 cut(s) 5, 77, 103, 297, 313
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 73, 406
DpnI GATC 2 cut(s) 179, 209
DpnII GATC 2 cut(s) 177, 207
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 5 cut(s) 19, 94, 284, 293, 333
FatI CATG 5 cut(s) 15, 90, 280, 289, 329
FblI GTMKAC 1 cut(s) 419
FokI GGATG 1 cut(s) 257
GlaI GCGC 2 cut(s) 220, 346
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 163
HgaI GACGC 2 cut(s) 133, 395
HhaI GCGC 2 cut(s) 221, 347
Hin1I GRCGYC 2 cut(s) 144, 387
Hin1II CATG 5 cut(s) 19, 94, 284, 293, 333
Hin6I GCGC 2 cut(s) 219, 345
HinP1I GCGC 2 cut(s) 219, 345
HinfI GANTC 1 cut(s) 157
HpaII CCGG 1 cut(s) 163
Hpy166II GTNNAC 3 cut(s) 96, 398, 420
Hpy188I TCNGA 1 cut(s) 409
Hpy8I GTNNAC 3 cut(s) 96, 398, 420
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 2 cut(s) 123, 424
HpyCH4V TGCA 3 cut(s) 119, 191, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 2 cut(s) 73, 406
Hsp92I GRCGYC 2 cut(s) 144, 387
Hsp92II CATG 5 cut(s) 19, 94, 284, 293, 333
HspAI GCGC 2 cut(s) 219, 345
Kzo9I GATC 2 cut(s) 177, 207
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 4 cut(s) 23, 139, 176, 436
LweI GCATC 1 cut(s) 241
MaeIII GTNAC 2 cut(s) 123, 182
MalI GATC 2 cut(s) 179, 209
MboI GATC 2 cut(s) 177, 207
MboII GAAGA 1 cut(s) 318
MflI RGATCY 1 cut(s) 177
MluCI AATT 1 cut(s) 105
MnlI CCTC 5 cut(s) 45, 134, 142, 235, 279
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 132, 257
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 221
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeII GATC 2 cut(s) 177, 207
NlaIII CATG 5 cut(s) 19, 94, 284, 293, 333
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 79, 164
NsiI ATGCAT 1 cut(s) 256
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 2 cut(s) 132, 257
Sau3AI GATC 2 cut(s) 177, 207
SetI ASST 2 cut(s) 37, 311
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 1 cut(s) 105
SsiI CCGC 1 cut(s) 136
TaaI ACNGT 2 cut(s) 123, 424
TaqI TCGA 2 cut(s) 147, 198
TasI AATT 1 cut(s) 105
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 132, 257
Tru9I TTAA 2 cut(s) 132, 257
TscAI CASTG 1 cut(s) 193
TspDTI ATGAA 3 cut(s) 4, 216, 318
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.