Rh6DG162900

Methylmalonate-semialdehyde dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0020295
Physical Location & Seq
Forward (+)
24621558 .. 24643193
21636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG162900.1

Sequence Viewer

Length: 360 bp
ATGTTCCTGTACATTTCGAGTTCCAACATTTTCCCATCTGTAGAAGCTCCAGCTCGCGTCTCATCTCGATTCTTCACTCCGGTGTCGCCCAGATCATTTTCTTCACTCCGGCGACGTTACCCAGTCCCCGGCCTATATGGCGATGGCTCTTCTATATTTACTACATCTGGGGTAGCTGCACGGAAATTTCAGACAGAGATTGAGGCTGGGCAGGTTGGTATCAACGTTCCTATTCCAGTTCCCTTTCCCTTTTTCTCTTTTACCGGCAACAAGGCATCCTTCGTAGGAGATTTGAACTTCTATGGCAAGGCAGGTGTCAACTTTTTCACCCAGATCAAGACAGTAACACAGCAATGGTGA

Protein Analysis

119

Amino Acids

13.12

Weight (kDa)

10.2

Isoelectric Point (pI)

64.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 46 - 115 2e-11 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11430
rosa_chinensis RchiOBHm_Chr6g0270071 RchiOBHm_Chr6g0270111
rosa_multiflora Rmu_sc0000918.1_g000020
rosa_samantha Rh6DG162900 Rh6DG163100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 2 cut(s) 202, 302
AccII CGCG 1 cut(s) 57
AclI AACGTT 1 cut(s) 225
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 295
AleI CACNNNNGTG 1 cut(s) 80
AluBI AGCT 3 cut(s) 47, 53, 176
AluI AGCT 3 cut(s) 47, 53, 176
Alw26I GTCTC 1 cut(s) 64
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 319
BbvI GCAGC 1 cut(s) 163
BccI CCATC 2 cut(s) 43, 137
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 64
BfmI CTRYAG 1 cut(s) 39
BfuAI ACCTGC 2 cut(s) 202, 302
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 127
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 122, 236
Bse3DI GCAATG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 275
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 122, 236
BseXI GCAGC 1 cut(s) 163
BseYI CCCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 132
BsiSI CCGG 4 cut(s) 80, 109, 129, 264
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 1 cut(s) 132
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 2 cut(s) 92, 333
BspANI GGCC 1 cut(s) 132
BspFNI CGCG 1 cut(s) 57
BspMI ACCTGC 2 cut(s) 202, 302
BspQI GCTCTTC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 2 cut(s) 122, 236
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 2 cut(s) 92, 333
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 55
BstF5I GGATG 1 cut(s) 275
BstFNI CGCG 1 cut(s) 57
BstKTI GATC 2 cut(s) 95, 336
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 2 cut(s) 92, 333
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 275
BveI ACCTGC 2 cut(s) 202, 302
Cac8I GCNNGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 263
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 10
CviJI RGCY 6 cut(s) 47, 53, 132, 147, 176, 206
CviKI_1 RGCY 6 cut(s) 47, 53, 132, 147, 176, 206
CviQI GTAC 1 cut(s) 10
DpnI GATC 2 cut(s) 94, 335
DpnII GATC 2 cut(s) 92, 333
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 64
FaiI YATR 4 cut(s) 136, 138, 155, 303
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 110
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 262
Fsp4HI GCNGC 1 cut(s) 177
GluI GCNGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 132
HapII CCGG 4 cut(s) 80, 109, 129, 264
HgaI GACGC 1 cut(s) 46
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 1 cut(s) 69
HpaII CCGG 4 cut(s) 80, 109, 129, 264
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 2 cut(s) 66, 337
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 2 cut(s) 115, 225
HpyCH4V TGCA 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpySE526I ACGT 2 cut(s) 115, 225
Kzo9I GATC 2 cut(s) 92, 333
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 284
MaeII ACGT 2 cut(s) 115, 225
MaeIII GTNAC 2 cut(s) 116, 343
MalI GATC 2 cut(s) 94, 335
MboI GATC 2 cut(s) 92, 333
MboII GAAGA 3 cut(s) 64, 93, 141
MluCI AATT 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 1 cut(s) 196
MslI CAYNNNNRTG 2 cut(s) 80, 352
MspI CCGG 4 cut(s) 80, 109, 129, 264
MspR9I CCNGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 138
NciI CCSGG 1 cut(s) 129
NdeII GATC 2 cut(s) 92, 333
OliI CACNNNNGTG 1 cut(s) 80
PaqCI CACCTGC 1 cut(s) 302
PciSI GCTCTTC 1 cut(s) 154
PfeI GAWTC 1 cut(s) 69
PkrI GCNGC 1 cut(s) 178
Psp1406I AACGTT 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 206
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 2 cut(s) 80, 352
SapI GCTCTTC 1 cut(s) 154
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 92, 333
ScrFI CCNGG 1 cut(s) 129
SetI ASST 7 cut(s) 49, 55, 118, 178, 216, 228, 316
SfaNI GCATC 1 cut(s) 284
SfcI CTRYAG 1 cut(s) 39
SmiMI CAYNNNNRTG 2 cut(s) 80, 352
Sse9I AATT 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 2 cut(s) 118, 228
TaqI TCGA 2 cut(s) 17, 67
TasI AATT 1 cut(s) 185
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 69
TseI GCWGC 1 cut(s) 176
TspGWI ACGGA 1 cut(s) 196
XapI RAATTY 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.