Rh7BG464400

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0023141
Physical Location & Seq
Reverse (-)
54974777 .. 54980075
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG464400.1

Sequence Viewer

Length: 627 bp
ATGAAAGGAACTGCAGGAAGAAGAACCCGACTCGAGGGACTCGATGTGCCGGCGTGCCGCTGCGCGTCCGAGGAACTGGTGCATGAAGCTATTGCACGAGAAGAGTCTCAGGAATTCCCAGAGGGGATTGTCATTCAAGAAATTCGCCGTGGCTTTTTACTTGGTGGTCAACTTCTAAGACCGGCAATGGTTAAAGTCTCTATAGGGCCTGGCAGTAAGAAGACCCCTGTGGCCACTGAGAAATCATCAGGGTCACCTGCAACAGCTGCTTCGGTACTAATGATGCTCTTGTGCTTCCTCGGGGTTCAGCTAGGGAATCTGATTCCTATTTCAGAAGTACAAGAAGTAGTAGCTGCATATAGAAAGGGCATGGAGTGGCATACAAGTGAGCTGCTTCTTCCACACAAGAAAATGTGTGATCTTAAGAAGGTGCAAGTTGATGTTGTAGTCATTGAGTCAGATAATGTGGCAAATGCAATAGCAGAGGAGTGCCCCATCTCGCGGCTTGTTTACAAGGAGGAGGTGAATGTCTCACCCAGGCACAGATCCCTTGGATTTCTTCCAAGAAAGCAAGTTGCTTGTCATAGAGGAAAAGCGAGCACTCCATGTAGTGAAGGCTTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

208

Amino Acids

22.67

Weight (kDa)

8.48

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 27 - 67 1.4e-10 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023141)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0020076.1_g000002
rosa_samantha Rh7BG464400 Rh7CG508300 Rh7DG475800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 265
AccII CGCG 2 cut(s) 65, 502
AciI CCGC 2 cut(s) 58, 502
AclWI GGATC 1 cut(s) 540
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 113, 141
AfaI GTAC 2 cut(s) 276, 339
AfiI CCNNNNNNNGG 2 cut(s) 34, 501
AflII CTTAAG 1 cut(s) 422
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 2 cut(s) 208, 536
AluBI AGCT 5 cut(s) 89, 266, 310, 353, 391
AluI AGCT 5 cut(s) 89, 266, 310, 353, 391
Alw21I GWGCWC 1 cut(s) 602
Alw26I GTCTC 3 cut(s) 111, 202, 535
AlwI GGATC 1 cut(s) 540
Ama87I CYCGRG 2 cut(s) 32, 299
AoxI GGCC 2 cut(s) 206, 231
ApeKI GCWGC 4 cut(s) 60, 266, 353, 391
ApoI RAATTY 2 cut(s) 113, 141
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 246, 525, 535
AvaI CYCGRG 2 cut(s) 32, 299
BaeGI GKGCMC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 233
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 602
BbvI GCAGC 4 cut(s) 47, 253, 340, 378
BccI CCATC 1 cut(s) 503
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 2 cut(s) 210, 538
BcoDI GTCTC 3 cut(s) 111, 202, 535
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 2 cut(s) 12, 201
BfrI CTTAAG 1 cut(s) 422
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 6 cut(s) 58, 61, 267, 354, 392, 503
BlsI GCNGC 6 cut(s) 59, 62, 268, 355, 393, 504
Bme1390I CCNGG 2 cut(s) 210, 538
BmeT110I CYCGRG 2 cut(s) 32, 299
BmgT120I GGNCC 1 cut(s) 206
BmrFI CCNGG 2 cut(s) 210, 538
BmsI GCATC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 227
BsaJI CCNNGG 5 cut(s) 69, 148, 298, 536, 550
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 501
Bse118I RCCGGY 2 cut(s) 49, 181
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 210, 538
BseDI CCNNGG 5 cut(s) 69, 148, 298, 536, 550
BseLI CCNNNNNNNGG 2 cut(s) 34, 501
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 122, 228
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 2 cut(s) 500, 533
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 4 cut(s) 47, 253, 340, 378
Bsh1236I CGCG 2 cut(s) 65, 502
BshFI GGCC 2 cut(s) 208, 233
BsiHKAI GWGCWC 1 cut(s) 602
BsiHKCI CYCGRG 2 cut(s) 32, 299
BsiSI CCGG 2 cut(s) 50, 182
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 34, 501
BsmAI GTCTC 3 cut(s) 111, 202, 535
BsmFI GGGAC 1 cut(s) 51
BsnI GGCC 2 cut(s) 208, 233
BsoBI CYCGRG 2 cut(s) 32, 299
Bsp1286I GDGCHC 2 cut(s) 494, 602
Bsp143I GATC 2 cut(s) 418, 545
BspACI CCGC 2 cut(s) 58, 502
BspANI GGCC 2 cut(s) 208, 233
BspCNI CTCAG 2 cut(s) 121, 229
BspFNI CGCG 2 cut(s) 65, 502
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 1 cut(s) 540
BspTI CTTAAG 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 49, 181
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 49, 181
BssECI CCNNGG 5 cut(s) 69, 148, 298, 536, 550
BssMI GATC 2 cut(s) 418, 545
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 550
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 210, 538
Bst6I CTCTTC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 422
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 51, 55, 598
BstDEI CTNAG 3 cut(s) 108, 176, 237
BstDSI CCRYGG 1 cut(s) 148
BstEII GGTNACC 1 cut(s) 252
BstFNI CGCG 2 cut(s) 65, 502
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 2 cut(s) 421, 548
BstMAI GTCTC 3 cut(s) 111, 202, 535
BstMBI GATC 2 cut(s) 418, 545
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNI CCWGG 2 cut(s) 210, 538
BstPI GGTNACC 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 208, 536
BstSFI CTRYAG 2 cut(s) 12, 201
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 2 cut(s) 65, 502
BstV1I GCAGC 4 cut(s) 47, 253, 340, 378
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BsuRI GGCC 2 cut(s) 208, 233
BtgI CCRYGG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 51, 55, 598
CfoI GCGC 1 cut(s) 65
Cfr10I RCCGGY 2 cut(s) 49, 181
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 54
Csp6I GTAC 2 cut(s) 275, 338
CviAII CATG 3 cut(s) 83, 370, 606
CviQI GTAC 2 cut(s) 275, 338
DdeI CTNAG 3 cut(s) 108, 176, 237
DpnI GATC 2 cut(s) 420, 547
DpnII GATC 2 cut(s) 418, 545
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 550
Eco88I CYCGRG 2 cut(s) 32, 299
Eco91I GGTNACC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 208, 536
EcoT14I CCWWGG 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 3 cut(s) 86, 373, 609
FaiI YATR 8 cut(s) 84, 203, 358, 360, 371, 381, 585, 607
FaqI GGGAC 1 cut(s) 51
FatI CATG 3 cut(s) 82, 369, 605
Fnu4HI GCNGC 6 cut(s) 58, 61, 267, 354, 392, 503
Fsp4HI GCNGC 6 cut(s) 58, 61, 267, 354, 392, 503
FspBI CTAG 1 cut(s) 311
GlaI GCGC 1 cut(s) 64
GluI GCNGC 6 cut(s) 58, 61, 267, 354, 392, 503
HaeIII GGCC 2 cut(s) 208, 233
HapII CCGG 2 cut(s) 50, 182
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 65
Hin1II CATG 3 cut(s) 86, 373, 609
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 6 cut(s) 30, 39, 104, 316, 322, 455
HpaII CCGG 2 cut(s) 50, 182
HphI GGTGA 3 cut(s) 246, 525, 535
Hpy166II GTNNAC 2 cut(s) 170, 511
Hpy188I TCNGA 4 cut(s) 70, 321, 334, 460
Hpy188III TCNNGA 2 cut(s) 110, 137
Hpy8I GTNNAC 2 cut(s) 170, 511
HpyAV CCTTC 2 cut(s) 421, 608
HpyCH4V TGCA 7 cut(s) 14, 82, 95, 260, 356, 433, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 3 cut(s) 108, 176, 237
Hsp92II CATG 3 cut(s) 86, 373, 609
HspAI GCGC 1 cut(s) 63
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 418, 545
Lsp1109I GCAGC 4 cut(s) 47, 253, 340, 378
LweI GCATC 1 cut(s) 273
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 252
MalI GATC 2 cut(s) 420, 547
MboI GATC 2 cut(s) 418, 545
MboII GAAGA 6 cut(s) 30, 33, 113, 232, 389, 551
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 2 cut(s) 494, 602
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 2 cut(s) 113, 141
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 4 cut(s) 24, 33, 113, 464
MnlI CCTC 8 cut(s) 28, 64, 115, 308, 478, 511, 514, 581
Mox20I TGGCCA 1 cut(s) 233
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 233
MseI TTAA 2 cut(s) 192, 423
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 2 cut(s) 60, 266
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 2 cut(s) 50, 182
MspR9I CCNGG 2 cut(s) 210, 538
MvaI CCWGG 2 cut(s) 210, 538
MvnI CGCG 2 cut(s) 65, 502
MwoI GCNNNNNNNGC 1 cut(s) 266
NaeI GCCGGC 1 cut(s) 51
NdeII GATC 2 cut(s) 418, 545
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 3 cut(s) 86, 373, 609
NmuCI GTSAC 1 cut(s) 252
PaeR7I CTCGAG 1 cut(s) 32
PaqCI CACCTGC 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 2 cut(s) 316, 322
PkrI GCNGC 6 cut(s) 59, 62, 268, 355, 393, 504
PleI GAGTC 4 cut(s) 24, 33, 112, 463
PpsI GAGTC 4 cut(s) 24, 33, 112, 463
Psp6I CCWGG 2 cut(s) 208, 536
PspEI GGTNACC 1 cut(s) 252
PspGI CCWGG 2 cut(s) 208, 536
PspPI GGNCC 1 cut(s) 206
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 2 cut(s) 276, 339
RsaNI GTAC 2 cut(s) 275, 338
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 2 cut(s) 192, 423
SatI GCNGC 6 cut(s) 58, 61, 267, 354, 392, 503
Sau3AI GATC 2 cut(s) 418, 545
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 4 cut(s) 24, 33, 113, 464
ScrFI CCNGG 2 cut(s) 210, 538
SduI GDGCHC 2 cut(s) 494, 602
SetI ASST 8 cut(s) 91, 259, 268, 312, 355, 393, 432, 525
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 2 cut(s) 12, 201
Sfr274I CTCGAG 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 32, 422
SmoI CTYRAG 2 cut(s) 32, 422
Sse9I AATT 2 cut(s) 113, 141
SsiI CCGC 2 cut(s) 58, 502
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 2 cut(s) 208, 536
StyI CCWWGG 1 cut(s) 550
TaqI TCGA 2 cut(s) 33, 42
TasI AATT 2 cut(s) 113, 141
TatI WGTACW 1 cut(s) 337
TauI GCSGC 2 cut(s) 60, 505
TfiI GAWTC 2 cut(s) 316, 322
Tru1I TTAA 2 cut(s) 192, 423
Tru9I TTAA 2 cut(s) 192, 423
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 4 cut(s) 60, 266, 353, 391
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 2 cut(s) 17, 99
TspRI CASTG 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 422
XapI RAATTY 2 cut(s) 113, 141
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.