Rh7DG032100

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012621
Physical Location & Seq
Forward (+)
2359014 .. 2361933
2920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG032100.1

Sequence Viewer

Length: 735 bp
ATGTCTGACACGTTCTGCCAAGCGTGCAAGTGTTTCACGGAGGTTGTTGTCTCCGACCACGCGACCGGCGGCACCTCATCGTGCTCCGAATGTGGGACTGTTGTGGTGGACGAGGTTCCCGAGTGGGTTTCTGTCCACGACCGGGAAGACCCGACCCGGGAGCCCAGCAAGCCGGTTGGACCCGAGAGTGGGGTTTCCGGGTCCAATGGAGCAGCAGCCTCCGACGACGAGGAGGCATCGCAGGCGCGGCGGGGGAAGCGGCGGAGGAGGCCACGCCCTCCGCCTTTTGACTGGTTTGGCTCTATTGCTGCCGTGTCTGACAGGTTGGGAATTGTTGCTGCCATAAAGGATCGAGCCAATGAGATATATAAGAAGGTGGTTGATCAGAAGCCTCTCAAATTTAGGACTCCGGATGCAGTTGTGGCTGCTTGCGTCTACATTGCCTGTAGACAAGAAAATAAGCCCCGTACTGTGAAAGAGATGTGCTCAGCAAATGGAGCTACCAAGAAAGAAGTTGCCAGGGCAAAAGACTTCATCATGAAATACTTGGAGACTGAGGGTCAGGAAATAGGAACTATTCATGCTGCAGACTTTCTGGTAAATCTGCTCAGAGAACTATTCATTTGTATCCCGATATGTTGTTTTCTTGTCGGAGATGTTCTTTGTGCTACAACACAATTTCCATCCATTTTCTGTAGAGACGTTTTTGTTCCGTTCTTGGAATGTCAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

244

Amino Acids

26.8

Weight (kDa)

5.75

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 101 - 173 1.8e-16 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 2 cut(s) 435, 448
AccII CGCG 2 cut(s) 62, 247
AccIII TCCGGA 1 cut(s) 409
AciI CCGC 6 cut(s) 69, 247, 250, 259, 262, 281
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 1 cut(s) 398
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 5 cut(s) 93, 142, 157, 188, 189
AflIII ACRYGT 1 cut(s) 9
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 1 cut(s) 518
AluBI AGCT 1 cut(s) 500
AluI AGCT 1 cut(s) 500
Alw21I GWGCWC 2 cut(s) 86, 488
Alw26I GTCTC 3 cut(s) 55, 545, 693
AlwI GGATC 1 cut(s) 357
Ama87I CYCGRG 3 cut(s) 119, 156, 182
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 1 cut(s) 269
ApeKI GCWGC 6 cut(s) 212, 215, 308, 338, 425, 584
ApoI RAATTY 1 cut(s) 398
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 179, 201
AsuC2I CCSGG 4 cut(s) 143, 157, 158, 199
AvaI CYCGRG 3 cut(s) 119, 156, 182
AvaII GGWCC 2 cut(s) 179, 201
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 86, 488
BbvI GCAGC 6 cut(s) 224, 227, 295, 325, 412, 571
BccI CCATC 1 cut(s) 691
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 520
BciVI GTATCC 1 cut(s) 638
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 4 cut(s) 143, 157, 158, 199
BcoDI GTCTC 3 cut(s) 55, 545, 693
BfmI CTRYAG 3 cut(s) 445, 585, 694
BfuI GTATCC 1 cut(s) 638
BisI GCNGC 9 cut(s) 70, 213, 216, 248, 260, 309, 339, 426, 585
BlpI GCTNAGC 1 cut(s) 487
BlsI GCNGC 9 cut(s) 71, 214, 217, 249, 261, 310, 340, 427, 586
Bme1390I CCNGG 5 cut(s) 143, 157, 158, 199, 520
Bme18I GGWCC 2 cut(s) 179, 201
BmeRI GACNNNNNGTC 1 cut(s) 558
BmeT110I CYCGRG 3 cut(s) 119, 156, 182
BmgT120I GGNCC 2 cut(s) 179, 201
BmiI GGNNCC 5 cut(s) 73, 117, 162, 181, 202
BmrFI CCNGG 5 cut(s) 143, 157, 158, 199, 520
BmsI GCATC 2 cut(s) 245, 403
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 470, 502
Bpu1102I GCTNAGC 1 cut(s) 487
BpuMI CCSGG 4 cut(s) 143, 157, 158, 199
BsaJI CCNNGG 2 cut(s) 156, 519
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 142, 157, 188, 189
Bse118I RCCGGY 2 cut(s) 65, 172
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 2 cut(s) 156, 519
BseGI GGATG 2 cut(s) 418, 683
BseLI CCNNNNNNNGG 5 cut(s) 93, 142, 157, 188, 189
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 3 cut(s) 501, 546, 622
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 2 cut(s) 245, 280
BseXI GCAGC 6 cut(s) 224, 227, 295, 325, 412, 571
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 2 cut(s) 62, 247
Bsh1285I CGRYCG 2 cut(s) 66, 142
BshFI GGCC 1 cut(s) 271
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 2 cut(s) 66, 142
BsiHKAI GWGCWC 2 cut(s) 86, 488
BsiHKCI CYCGRG 3 cut(s) 119, 156, 182
BsiSI CCGG 6 cut(s) 66, 142, 157, 173, 198, 410
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 5 cut(s) 93, 142, 157, 188, 189
BsmAI GTCTC 3 cut(s) 55, 545, 693
BsmBI CGTCTC 1 cut(s) 693
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 1 cut(s) 271
BsoBI CYCGRG 3 cut(s) 119, 156, 182
Bsp1286I GDGCHC 3 cut(s) 86, 165, 488
Bsp13I TCCGGA 1 cut(s) 409
Bsp143I GATC 2 cut(s) 349, 382
Bsp1720I GCTNAGC 1 cut(s) 487
BspACI CCGC 6 cut(s) 69, 247, 250, 259, 262, 281
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 3 cut(s) 500, 547, 621
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 2 cut(s) 62, 247
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 5 cut(s) 73, 117, 162, 181, 202
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 2 cut(s) 65, 172
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 2 cut(s) 65, 172
BssECI CCNNGG 2 cut(s) 156, 519
BssMI GATC 2 cut(s) 349, 382
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 2 cut(s) 100, 472
BstC8I GCNNGC 4 cut(s) 25, 170, 243, 430
BstDEI CTNAG 3 cut(s) 487, 555, 608
BstF5I GGATG 2 cut(s) 418, 683
BstFNI CGCG 2 cut(s) 62, 247
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 2 cut(s) 352, 385
BstMAI GTCTC 3 cut(s) 55, 545, 693
BstMBI GATC 2 cut(s) 349, 382
BstMCI CGRYCG 2 cut(s) 66, 142
BstMWI GCNNNNNNNGC 8 cut(s) 24, 169, 242, 247, 256, 268, 422, 497
BstNI CCWGG 1 cut(s) 520
BstSCI CCNGG 5 cut(s) 141, 155, 156, 197, 518
BstSFI CTRYAG 3 cut(s) 445, 585, 694
BstUI CGCG 2 cut(s) 62, 247
BstV1I GCAGC 6 cut(s) 224, 227, 295, 325, 412, 571
BstV2I GAAGAC 1 cut(s) 153
BsuI GTATCC 1 cut(s) 638
BsuRI GGCC 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 418, 683
Cac8I GCNNGC 4 cut(s) 25, 170, 243, 430
CciI TCATGA 1 cut(s) 537
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 2 cut(s) 65, 172
Cfr13I GGNCC 2 cut(s) 179, 201
Cfr9I CCCGGG 1 cut(s) 156
CseI GACGC 1 cut(s) 421
Csp6I GTAC 1 cut(s) 468
CviAII CATG 2 cut(s) 538, 581
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 3 cut(s) 487, 555, 608
DpnI GATC 2 cut(s) 351, 384
DpnII GATC 2 cut(s) 349, 382
DriI GACNNNNNGTC 1 cut(s) 558
Eam1105I GACNNNNNGTC 1 cut(s) 558
EciI GGCGGA 2 cut(s) 270, 277
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 179, 201
Eco88I CYCGRG 3 cut(s) 119, 156, 182
EcoRII CCWGG 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 693
FaeI CATG 2 cut(s) 541, 584
FaiI YATR 7 cut(s) 344, 367, 369, 539, 582, 637, 733
FaqI GGGAC 1 cut(s) 109
FatI CATG 2 cut(s) 537, 580
FauI CCCGC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 2 cut(s) 435, 448
Fnu4HI GCNGC 9 cut(s) 70, 213, 216, 248, 260, 309, 339, 426, 585
FokI GGATG 2 cut(s) 425, 670
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 9 cut(s) 70, 213, 216, 248, 260, 309, 339, 426, 585
GlaI GCGC 1 cut(s) 246
GluI GCNGC 9 cut(s) 70, 213, 216, 248, 260, 309, 339, 426, 585
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 1 cut(s) 271
HapII CCGG 6 cut(s) 66, 142, 157, 173, 198, 410
HgaI GACGC 1 cut(s) 421
HhaI GCGC 1 cut(s) 247
Hin1II CATG 2 cut(s) 541, 584
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 1 cut(s) 406
HpaII CCGG 6 cut(s) 66, 142, 157, 173, 198, 410
Hpy166II GTNNAC 4 cut(s) 109, 136, 436, 449
Hpy188I TCNGA 8 cut(s) 7, 55, 88, 223, 319, 387, 611, 653
Hpy188III TCNNGA 5 cut(s) 119, 410, 538, 563, 631
Hpy8I GTNNAC 4 cut(s) 109, 136, 436, 449
Hpy99I CGWCG 2 cut(s) 227, 230
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 2 cut(s) 100, 472
HpyCH4IV ACGT 2 cut(s) 11, 702
HpyCH4V TGCA 3 cut(s) 27, 416, 587
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 169, 242, 247, 256, 268, 422, 497
HpyF3I CTNAG 3 cut(s) 487, 555, 608
HpySE526I ACGT 2 cut(s) 11, 702
Hsp92II CATG 2 cut(s) 541, 584
HspAI GCGC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 2 cut(s) 349, 382
LmnI GCTCC 4 cut(s) 89, 160, 209, 497
Lsp1109I GCAGC 6 cut(s) 224, 227, 295, 325, 412, 571
LweI GCATC 2 cut(s) 245, 403
MaeII ACGT 2 cut(s) 11, 702
MalI GATC 2 cut(s) 351, 384
MboI GATC 2 cut(s) 349, 382
MboII GAAGA 1 cut(s) 158
MhlI GDGCHC 3 cut(s) 86, 165, 488
MluCI AATT 3 cut(s) 330, 398, 677
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 4 cut(s) 78, 157, 246, 631
MroI TCCGGA 1 cut(s) 409
MspI CCGG 6 cut(s) 66, 142, 157, 173, 198, 410
MspR9I CCNGG 5 cut(s) 143, 157, 158, 199, 520
MvaI CCWGG 1 cut(s) 520
MvnI CGCG 2 cut(s) 62, 247
MwoI GCNNNNNNNGC 8 cut(s) 24, 169, 242, 247, 256, 268, 422, 497
NciI CCSGG 4 cut(s) 143, 157, 158, 199
NdeII GATC 2 cut(s) 349, 382
NlaIII CATG 2 cut(s) 541, 584
NlaIV GGNNCC 5 cut(s) 73, 117, 162, 181, 202
PagI TCATGA 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PkrI GCNGC 9 cut(s) 71, 214, 217, 249, 261, 310, 340, 427, 586
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 518
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 5 cut(s) 73, 117, 162, 181, 202
PspPI GGNCC 2 cut(s) 179, 201
PstI CTGCAG 1 cut(s) 589
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SatI GCNGC 9 cut(s) 70, 213, 216, 248, 260, 309, 339, 426, 585
Sau3AI GATC 2 cut(s) 349, 382
Sau96I GGNCC 2 cut(s) 179, 201
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 5 cut(s) 143, 157, 158, 199, 520
SduI GDGCHC 3 cut(s) 86, 165, 488
SetI ASST 8 cut(s) 14, 45, 77, 117, 326, 378, 502, 705
SfaNI GCATC 2 cut(s) 245, 403
SfcI CTRYAG 3 cut(s) 445, 585, 694
SinI GGWCC 2 cut(s) 179, 201
SmaI CCCGGG 1 cut(s) 158
Sse9I AATT 3 cut(s) 330, 398, 677
SsiI CCGC 6 cut(s) 69, 247, 250, 259, 262, 281
StyD4I CCNGG 5 cut(s) 141, 155, 156, 197, 518
TaaI ACNGT 2 cut(s) 100, 472
TaiI ACGT 2 cut(s) 14, 705
TaqI TCGA 1 cut(s) 352
TasI AATT 3 cut(s) 330, 398, 677
TauI GCSGC 3 cut(s) 72, 250, 262
TseI GCWGC 6 cut(s) 212, 215, 308, 338, 425, 584
TspDTI ATGAA 4 cut(s) 523, 554, 569, 610
TspGWI ACGGA 2 cut(s) 53, 702
TspMI CCCGGG 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 179, 201
XapI RAATTY 1 cut(s) 398
XmaI CCCGGG 1 cut(s) 156
XmiI GTMKAC 2 cut(s) 435, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.