Rh7DG070200

AtCASP,CASP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0021468
Physical Location & Seq
Forward (+)
5401602 .. 5407099
5498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG070200.1

Sequence Viewer

Length: 342 bp
ATGACATCATATTTCAGTGCGACGGATTCCCAGTTAGCGGAGTTTTGGAGGGATGTAGCTCCAAGGATAAAAAGGGAAATTTTATTTGATGACCAGGATCTATCAGAGGCTAGGAGCACAGACCTATCAGAGAATGCAGAACAAAAGCATGTTTCCTCAGACCAAGACCAAAGCTCAATGCTTAAGGTTATCTGCAATCAGCTAGATCGATTCAGGGCATGCTTGAGAGAGGCAGAAGAAGAAATTAGACAGTTGAAGGAAAAGATAGGAGCTCTAACAGTGGAACTGGAAAGGACAAAAGCTGATAATGCCATGTTCAGGACTACAGTCTTGAAAAAGTAG

Protein Analysis

113

Amino Acids

13.12

Weight (kDa)

5.09

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_C PF08172 39 - 104 1.2e-11 CASP C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021468)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0184881 RchiOBHm_Chr7g0184901
rosa_samantha Rh7AG077300 Rh7CG070900 Rh7DG070200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 38
AclWI GGATC 1 cut(s) 105
AcsI RAATTY 1 cut(s) 78
AfiI CCNNNNNNNGG 2 cut(s) 37, 318
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 2 cut(s) 256, 334
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 5 cut(s) 59, 174, 202, 272, 302
AluI AGCT 5 cut(s) 59, 174, 202, 272, 302
Alw21I GWGCWC 2 cut(s) 119, 274
AlwI GGATC 1 cut(s) 105
ApoI RAATTY 1 cut(s) 78
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 119, 274
BciT130I CCWGG 1 cut(s) 95
BfaI CTAG 2 cut(s) 111, 203
BfmI CTRYAG 1 cut(s) 324
BfrI CTTAAG 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 95
BmrI ACTGGG 1 cut(s) 25
BmuI ACTGGG 1 cut(s) 25
BoxI GACNNNNGTC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 244
Bsa29I ATCGAT 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 62
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 318
Bse1I ACTGG 2 cut(s) 31, 291
BseBI CCWGG 1 cut(s) 95
BseCI ATCGAT 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 62
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 2 cut(s) 37, 318
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 2 cut(s) 31, 291
BshVI ATCGAT 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 119, 274
BslI CCNNNNNNNGG 2 cut(s) 37, 318
BsmI GAATGC 1 cut(s) 139
Bsp1286I GDGCHC 2 cut(s) 119, 274
Bsp143I GATC 2 cut(s) 97, 205
BspACI CCGC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 170
BspDI ATCGAT 1 cut(s) 208
BspPI GGATC 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 182
BsrI ACTGG 2 cut(s) 31, 291
BssECI CCNNGG 1 cut(s) 62
BssMI GATC 2 cut(s) 97, 205
BssT1I CCWWGG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 252, 280, 328
BstAFI CTTAAG 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 58
BstKTI GATC 2 cut(s) 100, 208
BstMBI GATC 2 cut(s) 97, 205
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 2 cut(s) 152, 222
BstPAI GACNNNNGTC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 208
BsuTUI ATCGAT 1 cut(s) 208
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 22, 285
Cac8I GCNNGC 1 cut(s) 220
ClaI ATCGAT 1 cut(s) 208
CviAII CATG 3 cut(s) 149, 219, 313
CviJI RGCY 6 cut(s) 59, 110, 174, 202, 272, 302
CviKI_1 RGCY 6 cut(s) 59, 110, 174, 202, 272, 302
DdeI CTNAG 1 cut(s) 157
DpnI GATC 2 cut(s) 99, 207
DpnII GATC 2 cut(s) 97, 205
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 3 cut(s) 152, 222, 316
FaiI YATR 4 cut(s) 10, 150, 220, 314
FatI CATG 3 cut(s) 148, 218, 312
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 274
FspBI CTAG 2 cut(s) 111, 203
Hin1II CATG 3 cut(s) 152, 222, 316
HinfI GANTC 2 cut(s) 26, 210
Hpy188I TCNGA 3 cut(s) 106, 130, 160
Hpy188III TCNNGA 2 cut(s) 319, 331
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 3 cut(s) 252, 280, 328
HpyCH4V TGCA 2 cut(s) 137, 195
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 157
Hsp92II CATG 3 cut(s) 152, 222, 316
Kzo9I GATC 2 cut(s) 97, 205
LmnI GCTCC 3 cut(s) 64, 114, 269
LpnPI CCDG 6 cut(s) 44, 80, 107, 199, 272, 304
MaeI CTAG 2 cut(s) 111, 203
MalI GATC 2 cut(s) 99, 207
MboI GATC 2 cut(s) 97, 205
MboII GAAGA 2 cut(s) 248, 251
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 2 cut(s) 119, 274
MluCI AATT 2 cut(s) 78, 243
MnlI CCTC 4 cut(s) 42, 100, 166, 223
MseI TTAA 1 cut(s) 183
MspCI CTTAAG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 139
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 2 cut(s) 97, 205
NlaIII CATG 3 cut(s) 152, 222, 316
NspI RCATGY 2 cut(s) 152, 222
PaeI GCATGC 1 cut(s) 222
PctI GAATGC 1 cut(s) 139
PfeI GAWTC 2 cut(s) 26, 210
PshAI GACNNNNGTC 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PsuI RGATCY 1 cut(s) 97
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 183
Sau3AI GATC 2 cut(s) 97, 205
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 119, 274
SetI ASST 7 cut(s) 61, 126, 176, 189, 204, 274, 304
SfcI CTRYAG 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 182, 223
SmoI CTYRAG 2 cut(s) 182, 223
SphI GCATGC 1 cut(s) 222
Sse9I AATT 2 cut(s) 78, 243
SsiI CCGC 1 cut(s) 38
SspMI CTAG 2 cut(s) 111, 203
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 3 cut(s) 252, 280, 328
TaqI TCGA 1 cut(s) 208
TasI AATT 2 cut(s) 78, 243
TfiI GAWTC 2 cut(s) 26, 210
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 22, 285
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 2 cut(s) 22, 285
Vha464I CTTAAG 1 cut(s) 182
XapI RAATTY 1 cut(s) 78
XceI RCATGY 2 cut(s) 152, 222
XspI CTAG 2 cut(s) 111, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.