Rw1G015190

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018540
Physical Location & Seq
Forward (+)
33924233 .. 33928695
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015190.1

Sequence Viewer

Length: 1080 bp
ATGGATTTGCCCATCTCCGGTCAGTTCATGCAGACTGCGAGTGAGGCATCACCAGGAGCTCGAAAATTTAACACTTACGACGAGGCCGGAGGTCTAGGAATTGCTACTATGACAATTGGCGGACCACATGAAAAGTCATTTAATAATTCAATCTCTGTGCAACACATGCAGGAGCTTTTCCGTTATCTGCAATTTGGACTCTGGTGGCTGGCTCTTGGTGTGGTGTCATCAATTGGTCTTGCTTTGTTTACCATGAAAGCCATGCAGTGTGGCAGAGTTGACTTAAAAATTGCCCCACATGTTACCGTTCAATTGAAAACAGGTCCTTCATGGATGGATAGAAACTGCTCTGAATATGGTCCCCTACTATTTTCATCCCAGCAAGGTTCAAGGCTCCCACTTAGCAGCATACTACCTCAGGTTCAGTTAGAGTCTATTCTAGGGGGAATTGGAACTGCACTGGGAGAGCTTCCTCCATTTTTCATATCAAGAGCTGCTCGTACAGCAGGGAGTGAATTGGAAGTAATGAAAGAATTGGATTCTTCTTCAACTAAAGATGGTGGAGTTGTAGCTAATCACCAGAACTGGATCAAACTATATATGGCTTCTTTCACATTCACAGTATCTGAACTTTTTAACAATATTAGTTCTTGCTTCGGTAAGGTACCCAACCCTCTAGGTGACCTTGCTGGTATAATGTGTGGACAACTTGGCATTCCATTTTGGACATTCTTTATCTCAACAATGACTGGAAATGCATTTATCAAGACTCACATACATACATGGTCCAAATCACACAACATATTTCTACCGTTATGTGATGCAGTTTTTAGTAGTGTGTTGCACAAATATCATTTAAACAAACAAATAAAGAGAGTTTATAAAAAAAAAACTTTTTTTTTAACGCTACCCCACCCCTCCCCTCCCATGATACCAGTGACGTCTAAAACTGTGACATCCATGATATCGGTTATCCTAGATAACCAACAAATCACAGCTCACCAACAACAACAACAACAAAAAGTCACTTCTTACCTTTACAAACAGTATGTTTTGATGAGACAATTTGCAAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 map04140
Pfam Domains
Protein Families
No domains found.

Protein Analysis

359

Amino Acids

39.87

Weight (kDa)

9.35

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AatII GACGTC 1 cut(s) 944
Acc65I GGTACC 1 cut(s) 664
AccB1I GGYRCC 1 cut(s) 664
AciI CCGC 1 cut(s) 120
AclWI GGATC 1 cut(s) 596
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 941
AfaI GTAC 2 cut(s) 502, 666
AfiI CCNNNNNNNGG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 5 cut(s) 150, 311, 316, 390, 549
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 6 cut(s) 59, 175, 469, 494, 572, 998
AluI AGCT 6 cut(s) 59, 175, 469, 494, 572, 998
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 1054
AlwI GGATC 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 2 cut(s) 405, 494
ApoI RAATTY 1 cut(s) 65
Asp718I GGTACC 1 cut(s) 664
AspS9I GGNCC 4 cut(s) 122, 323, 359, 786
AsuHPI GGTGA 4 cut(s) 42, 569, 692, 992
AvaII GGWCC 4 cut(s) 122, 323, 359, 786
AxyI CCTNAGG 1 cut(s) 417
BanI GGYRCC 1 cut(s) 664
BanII GRGCYC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 417, 481
BccI CCATC 3 cut(s) 20, 328, 551
BciT130I CCWGG 1 cut(s) 54
BcoDI GTCTC 1 cut(s) 1054
BfaI CTAG 4 cut(s) 95, 440, 677, 977
BisI GCNGC 2 cut(s) 406, 495
BlsI GCNGC 2 cut(s) 407, 496
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 4 cut(s) 122, 323, 359, 786
BmgT120I GGNCC 4 cut(s) 122, 323, 359, 786
BmiI GGNNCC 3 cut(s) 361, 395, 666
BmrFI CCNGG 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 2 cut(s) 56, 811
BmuI ACTGGG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 941
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 4 cut(s) 465, 590, 754, 935
Bse21I CCTNAGG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 54
BseGI GGATG 3 cut(s) 339, 374, 956
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 4 cut(s) 465, 590, 754, 935
BseXI GCAGC 2 cut(s) 417, 481
BseYI CCCAGC 1 cut(s) 378
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 61
BsiSI CCGG 2 cut(s) 18, 87
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 1054
BsmFI GGGAC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 588
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 361, 395, 666
BspPI GGATC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 664
BsrI ACTGG 4 cut(s) 465, 590, 754, 935
BssMI GATC 1 cut(s) 588
BssNI GRCGYC 1 cut(s) 941
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 5 cut(s) 307, 622, 813, 952, 1047
BstACI GRCGYC 1 cut(s) 941
BstAPI GCANNNNNTGC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 401, 417
BstEII GGTNACC 1 cut(s) 680
BstF5I GGATG 3 cut(s) 339, 374, 956
BstKTI GATC 1 cut(s) 591
BstMAI GTCTC 1 cut(s) 1054
BstMBI GATC 1 cut(s) 588
BstMWI GCNNNNNNNGC 3 cut(s) 44, 166, 503
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 2 cut(s) 169, 302
BstPI GGTNACC 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 417, 481
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 3 cut(s) 339, 374, 956
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 3 cut(s) 272, 458, 942
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 1 cut(s) 626
Cfr13I GGNCC 4 cut(s) 122, 323, 359, 786
Csp6I GTAC 2 cut(s) 501, 665
CviQI GTAC 2 cut(s) 501, 665
DdeI CTNAG 2 cut(s) 401, 417
DpnI GATC 1 cut(s) 590
DpnII GATC 1 cut(s) 588
DraI TTTAAA 1 cut(s) 858
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 966
Eco47I GGWCC 4 cut(s) 122, 323, 359, 786
Eco53kI GAGCTC 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 417
Eco91I GGTNACC 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 52
EcoRV GATATC 1 cut(s) 966
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 1 cut(s) 61
FaqI GGGAC 1 cut(s) 345
Fnu4HI GCNGC 2 cut(s) 406, 495
FokI GGATG 3 cut(s) 346, 361, 943
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 406, 495
FspBI CTAG 4 cut(s) 95, 440, 677, 977
GluI GCNGC 2 cut(s) 406, 495
GsaI CCCAGC 1 cut(s) 382
HaeIII GGCC 1 cut(s) 86
HapII CCGG 2 cut(s) 18, 87
Hin1I GRCGYC 1 cut(s) 941
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 4 cut(s) 198, 431, 539, 769
HpaII CCGG 2 cut(s) 18, 87
HphI GGTGA 4 cut(s) 42, 569, 692, 992
Hpy166II GTNNAC 3 cut(s) 249, 280, 704
Hpy188I TCNGA 2 cut(s) 352, 628
Hpy188III TCNNGA 2 cut(s) 489, 766
Hpy8I GTNNAC 3 cut(s) 249, 280, 704
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 5 cut(s) 307, 622, 813, 952, 1047
HpyCH4IV ACGT 1 cut(s) 941
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 166, 503
HpyF3I CTNAG 2 cut(s) 401, 417
HpySE526I ACGT 1 cut(s) 941
Hsp92I GRCGYC 1 cut(s) 941
KpnI GGTACC 1 cut(s) 668
Kzo9I GATC 1 cut(s) 588
LmnI GCTCC 3 cut(s) 56, 172, 399
Lsp1109I GCAGC 2 cut(s) 417, 481
LweI GCATC 2 cut(s) 56, 811
MaeI CTAG 4 cut(s) 95, 440, 677, 977
MaeII ACGT 1 cut(s) 941
MaeIII GTNAC 5 cut(s) 301, 680, 937, 952, 1024
MalI GATC 1 cut(s) 590
MboI GATC 1 cut(s) 588
MboII GAAGA 2 cut(s) 534, 537
MfeI CAATTG 3 cut(s) 114, 231, 311
MhlI GDGCHC 1 cut(s) 61
MlyI GAGTC 3 cut(s) 192, 440, 763
MnlI CCTC 8 cut(s) 37, 76, 83, 426, 483, 684, 928, 933
Mph1103I ATGCAT 1 cut(s) 760
MseI TTAA 6 cut(s) 69, 141, 284, 636, 857, 902
MspI CCGG 2 cut(s) 18, 87
MspR9I CCNGG 1 cut(s) 54
MunI CAATTG 3 cut(s) 114, 231, 311
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 3 cut(s) 44, 166, 503
NdeII GATC 1 cut(s) 588
NlaIV GGNNCC 3 cut(s) 361, 395, 666
NmuCI GTSAC 4 cut(s) 680, 937, 952, 1024
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 2 cut(s) 169, 302
PciI ACATGT 1 cut(s) 298
PctI GAATGC 1 cut(s) 714
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 2 cut(s) 407, 496
PleI GAGTC 3 cut(s) 192, 439, 763
PpsI GAGTC 3 cut(s) 192, 439, 763
PpuMI RGGWCCY 1 cut(s) 323
PscI ACATGT 1 cut(s) 298
PsiI TTATAA 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 52
PspEI GGTNACC 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 378
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 361, 395, 666
PspPI GGNCC 4 cut(s) 122, 323, 359, 786
PspPPI RGGWCCY 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 502, 666
RsaNI GTAC 2 cut(s) 501, 665
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 6 cut(s) 69, 141, 284, 636, 857, 902
SatI GCNGC 2 cut(s) 406, 495
Sau3AI GATC 1 cut(s) 588
Sau96I GGNCC 4 cut(s) 122, 323, 359, 786
SchI GAGTC 3 cut(s) 192, 440, 763
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 61
SfaNI GCATC 2 cut(s) 56, 811
SinI GGWCC 4 cut(s) 122, 323, 359, 786
SsiI CCGC 1 cut(s) 120
SspI AATATT 1 cut(s) 643
SspMI CTAG 4 cut(s) 95, 440, 677, 977
SstI GAGCTC 1 cut(s) 61
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 5 cut(s) 307, 622, 813, 952, 1047
TaiI ACGT 1 cut(s) 944
TaqI TCGA 1 cut(s) 61
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 6 cut(s) 69, 141, 284, 636, 857, 902
Tru9I TTAA 6 cut(s) 69, 141, 284, 636, 857, 902
TscAI CASTG 3 cut(s) 272, 465, 942
TseFI GTSAC 4 cut(s) 680, 937, 952, 1024
TseI GCWGC 2 cut(s) 405, 494
Tsp45I GTSAC 4 cut(s) 680, 937, 952, 1024
TspDTI ATGAA 7 cut(s) 16, 144, 269, 318, 363, 472, 542
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 272, 465, 942
VpaK11BI GGWCC 4 cut(s) 122, 323, 359, 786
XapI RAATTY 1 cut(s) 65
XceI RCATGY 2 cut(s) 169, 302
XspI CTAG 4 cut(s) 95, 440, 677, 977
ZraI GACGTC 1 cut(s) 942
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.