Rw1G018930

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0021654
Physical Location & Seq
Forward (+)
41480366 .. 41481227
862 bp
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UTR
Exon/CDS
Intron
Rw1G018930.1

Sequence Viewer

Length: 552 bp
ATGGCTGCTCAAACAAAGGTCTTCTTGGACATGAAGATGGGAGGCCAGCCGGCCGGCCGCATCGTCATGGAGCTCTACGCCGACTCTACCCCCCGTACCGCCGAGAACTTCCGTGCTCTCTGCACCGGCGAGAAGGGCGTAGACCGCTCGGGTAGGACCTCCACCCCCCGCGTCATCAAGAACATCAAGGAGGTTAAGTCTAGCCCTGGAAGGACCGCCAAGAACGCCAAGAACATCGAGAAGGTTGCGTCTAGCCCTCGAAGGACCGCCGAGAACATCGAGAAGGTTAGGACCGCCAAGAACATCGAGAAGGTTAGGACTGCCATGAAGGTTGTGTCTATTGCTGGAAGGACCGTCAAGAACATCGAGAAGGTTAGGACCGCTGAGAAGGTTAGGACCGCCGAGAAGGTTGTGACCGTCAAGAATATCGAGAAGGTTAGGACCGCCGAGAAGGTTGTGTCGACCGTCAAGAACGTCGAGAAGGTTAGGACCGCCAAGAAGGTTGGGTCTAGCGCTGGAAGGACCGCCAAGCCTGTTGTGATTGCCTACTGA

Protein Analysis

183

Amino Acids

20.0

Weight (kDa)

10.67

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 8 - 67 5.4e-07 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021654)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002495.1_g000039
rosa_samantha Rh1AG222200 Rh1CG207000
rosa_wichuraiana Rw0G014810 Rw1G018930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 147
AccI GTMKAC 2 cut(s) 141, 461
AccII CGCG 1 cut(s) 171
AcoI YGGCCR 2 cut(s) 51, 55
AfaI GTAC 1 cut(s) 97
AfeI AGCGCT 1 cut(s) 514
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw21I GWGCWC 2 cut(s) 75, 118
Ama87I CYCGRG 1 cut(s) 148
Aor51HI AGCGCT 1 cut(s) 514
AoxI GGCC 3 cut(s) 43, 51, 55
ApeKI GCWGC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 148
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 2 cut(s) 75, 118
BccI CCATC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 207
BfaI CTAG 3 cut(s) 201, 252, 510
BfoI RGCGCY 1 cut(s) 516
BisI GCNGC 2 cut(s) 6, 58
BlsI GCNGC 2 cut(s) 7, 59
Bme1390I CCNGG 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 205
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bse118I RCCGGY 3 cut(s) 49, 53, 125
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 375
BseX3I CGGCCG 2 cut(s) 51, 55
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 171
Bsh1285I CGRYCG 3 cut(s) 54, 58, 465
BshFI GGCC 3 cut(s) 45, 53, 57
BsiEI CGRYCG 3 cut(s) 54, 58, 465
BsiHKAI GWGCWC 2 cut(s) 75, 118
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 3 cut(s) 50, 54, 126
BsnI GGCC 3 cut(s) 45, 53, 57
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 75, 118
BspANI GGCC 3 cut(s) 45, 53, 57
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 147
BsrFI RCCGGY 3 cut(s) 49, 53, 125
BssAI RCCGGY 3 cut(s) 49, 53, 125
BssECI CCNNGG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 3 cut(s) 355, 418, 466
BstC8I GCNNGC 3 cut(s) 47, 51, 55
BstDEI CTNAG 1 cut(s) 384
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstMCI CGRYCG 3 cut(s) 54, 58, 465
BstMWI GCNNNNNNNGC 3 cut(s) 135, 144, 224
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 13
BstZI CGGCCG 2 cut(s) 51, 55
BsuRI GGCC 3 cut(s) 45, 53, 57
Cac8I GCNNGC 3 cut(s) 47, 51, 55
CfoI GCGC 1 cut(s) 515
Cfr10I RCCGGY 3 cut(s) 49, 53, 125
CseI GACGC 2 cut(s) 160, 237
Csp6I GTAC 1 cut(s) 96
CviAII CATG 3 cut(s) 31, 67, 325
CviJI RGCY 9 cut(s) 5, 45, 49, 53, 57, 73, 204, 255, 532
CviKI_1 RGCY 9 cut(s) 5, 45, 49, 53, 57, 73, 204, 255, 532
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 1 cut(s) 384
EaeI YGGCCR 2 cut(s) 51, 55
EagI CGGCCG 2 cut(s) 51, 55
Ecl136II GAGCTC 1 cut(s) 73
EclXI CGGCCG 2 cut(s) 51, 55
Eco24I GRGCYC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 514
Eco52I CGGCCG 2 cut(s) 51, 55
Eco53kI GAGCTC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 75
FaeI CATG 3 cut(s) 34, 70, 328
FaiI YATR 3 cut(s) 32, 68, 326
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FatI CATG 3 cut(s) 30, 66, 324
FauI CCCGC 1 cut(s) 176
FblI GTMKAC 2 cut(s) 141, 461
Fnu4HI GCNGC 2 cut(s) 6, 58
FriOI GRGCYC 1 cut(s) 75
FseI GGCCGGCC 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 6, 58
FspBI CTAG 3 cut(s) 201, 252, 510
GlaI GCGC 1 cut(s) 514
GluI GCNGC 2 cut(s) 6, 58
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 3 cut(s) 45, 53, 57
HapII CCGG 3 cut(s) 50, 54, 126
HgaI GACGC 2 cut(s) 160, 237
HhaI GCGC 1 cut(s) 515
Hin1II CATG 3 cut(s) 34, 70, 328
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HinfI GANTC 1 cut(s) 83
HpaII CCGG 3 cut(s) 50, 54, 126
Hpy166II GTNNAC 2 cut(s) 142, 462
Hpy8I GTNNAC 2 cut(s) 142, 462
Hpy99I CGWCG 1 cut(s) 479
HpyCH4III ACNGT 3 cut(s) 355, 418, 466
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 144, 224
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 3 cut(s) 34, 70, 328
HspAI GCGC 1 cut(s) 513
KroI GCCGGC 2 cut(s) 49, 53
KroNI GCCGGC 2 cut(s) 51, 55
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 9 cut(s) 59, 63, 67, 139, 192, 219, 330, 501, 546
LweI GCATC 1 cut(s) 69
MaeI CTAG 3 cut(s) 201, 252, 510
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 1 cut(s) 412
MbiI CCGCTC 1 cut(s) 147
MboII GAAGA 2 cut(s) 13, 46
MhlI GDGCHC 2 cut(s) 75, 118
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 4 cut(s) 35, 169, 184, 267
MroNI GCCGGC 2 cut(s) 49, 53
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 2 cut(s) 35, 65
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 3 cut(s) 50, 54, 126
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 135, 144, 224
NaeI GCCGGC 2 cut(s) 51, 55
NgoMIV GCCGGC 2 cut(s) 49, 53
NlaIII CATG 3 cut(s) 34, 70, 328
NmeAIII GCCGAG 4 cut(s) 127, 295, 427, 472
NmuCI GTSAC 1 cut(s) 412
PdiI GCCGGC 2 cut(s) 51, 55
PkrI GCNGC 2 cut(s) 7, 59
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspPPI RGGWCCY 1 cut(s) 156
RigI GGCCGGCC 1 cut(s) 57
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 35, 65
SacI GAGCTC 1 cut(s) 75
SalI GTCGAC 1 cut(s) 460
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 6, 58
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 75, 118
SfaNI GCATC 1 cut(s) 69
SgrAI CRCCGGYG 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 35, 65
SspMI CTAG 3 cut(s) 201, 252, 510
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 3 cut(s) 355, 418, 466
TaiI ACGT 1 cut(s) 477
TaqI TCGA 8 cut(s) 237, 259, 279, 306, 366, 429, 461, 477
TauI GCSGC 1 cut(s) 60
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 47, 341
TspGWI ACGGA 1 cut(s) 101
XmiI GTMKAC 2 cut(s) 141, 461
XspI CTAG 3 cut(s) 201, 252, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.