Rw1G019000

Nitroreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012061
Physical Location & Seq
Reverse (-)
41556385 .. 41559598
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G019000.1

Sequence Viewer

Length: 1740 bp
ATGCCACCTCTCCCTCCCACAAGGTTCTCCTTCCCTCTAACCTCTCCACTCCTCCACCTCCCCTCCCACCACACCACCACCACCACCATGTCCTTCTCTTCCTCTTCTCCTTCCCCTTCTTCACTCTCCAACCCCAAACAACCCGACCCACAACTCGACCAAAACCTCTCGCAGGTCCTCACGTACCACAACGACACCAAGCACCACTTCACCCGCTACGCCAGAAGCCCTCGTGGCCTCGACTGGGCCAACCAGCCCAACCCATTCCGCCGCTACCTCTCCTCCCCTCTCCTCCCTCTCCTCCACCCCACACCACCCGACGACCACCCCTCCCCTCTCTACTCCTCCCTCTTCACCTCTCTCCCTCCCCCAAAACCCATCTCCAAATCCACCATTTCCCAACTCCTCTACGACTCTCTAGCTCTCTCCGCTTGGAAAACCACCGGCTTCTCCACCTGGTCCCTCAGAGTCAATCCCAGCAGCGGTAATCTACACCCCACCGAGGCTTATATAATCTCCCCGCCGATCCATTCCCTCTCCGATTCCGGCTTCGTGGCCCATTACGCTCCCAAAGAGCACGCATTGGAGCTCCGGGCCCAAATCCCAACCTGGGTCTTCGCCGATTTGCTTCCCAAAGGCTCGTTCTTGGTCGGACTATCGTCGATTTTTTGGCGGGAAGCTTGGAAGTACGGCGAGCGGGCTTTCCGGTATTGTAACCACGATGTGGGCCATGCTATTGCGGCGGTTGCGGTGGCGGCGGCGGAGCTGGGTTGGGATATGTTGCCACATTTCGAAATTCCGGCTCGGGCTGTGAAGGGGAGGCTTCCCGAGATGGAATTCGAGCACCCGGATTGTGTTCTGGTGGTTTTTCCTAGTGGACGTGGTGGGTTTGAGGTGGATTATGCGAAGTTGAGCTTGGCGATTTCGGAGTTTTCAAAGTTGGAGTGGAAGGGGAAGCCGAATGTGCTTAGTAAAGAGCATATATGTTGGGATGTGATATATAGAACGGCCGAGGCTGTGAAGAAAGAGAGGGATATAGGACGTAGGTTTTCGGTTGATGCGTTTCGGAGTAGTGGGAGTTGTAGTGAGGGTTCGTATAAGGGTTTTACGGTTAGGGAGGTTGTTAGGAAGCGGAGGAGTGCTGTGGATATGGATGGAATTCTTTTGCATTGTCTTCCTTCGGGAAGTGGAGGGAAGCAGAAGCGGCAGCTGGCATTGCCGTTTCGGGCTCTTTCGGTGAAGGGACTGCCGGAGGGATTGTATTTCTTGGTGAGGAATGAGGATCATTTTGATGAGCTCAAGAAATCTATGAGGTCTGGGTTTAAGTGGGTGAAACCTCAGGGGTGCCCGGAAGGTCTTCCCCTGTACGAACTGCATAGAACTGATTGTCGGGCTATTGCTGAAAAGCTCTCGTGCCATCAGGAAATTGCCAGTCATGGCTGCTTTAGCCTCGGCATGGTGGCTTCTTTTGAGCCTCTTTTGCAGGACAAAAATGTGTGGATGTATCCTCGATTATTTTGGGAGACTGGAGTTCTCGGTCAAGTGTTGTACCTTGAAGCACATGCAATTGGCATTTCTGCAACAGGAATTGGTTGCTATTTTGATGATCCTGTGCATGAATTGCTCGGGTTGAAAGGATCGAACTTCCAGAGTCTGTATCATTTCACAGTGGGAGGCCCAGTGATTGACAAGCGGATTATGAGCCTACCGGCATATCCTGGCCCTAGTGTTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nitroreductase

Protein Analysis

579

Amino Acids

64.57

Weight (kDa)

8.53

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nitroreductase PF00881 124 - 272 5.8e-06 Nitroreductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012061)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02020 AT1G02020 AT1G02020
fragaria_vesca FvH4_7g11430 FvH4_7g11430
malus_domestica MD07G1119000.v1.1
prunus_persica Prupe.2G142200_v2.0.a1
pyrus_communis pycom07g10560
rosa_chinensis RchiOBHm_Chr1g0349711
rosa_laevigata RLG00000028532
rosa_multiflora Rmu_sc0004624.1_g000015 Rmu_sc0033954.1_g000001
rosa_roxburghii Rroxscaffold_4G00305360
rosa_rugosa Rorug01G0205500
rosa_samantha Rh1AG222800 Rh1BG188900 Rh1CG207500 Rh1DG218000
rosa_wichuraiana Rw1G019000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 1344
AccB7I CCANNNNNTGG 1 cut(s) 724
AccBSI CCGCTC 1 cut(s) 697
AclWI GGATC 4 cut(s) 520, 1290, 1601, 1645
AcoI YGGCCR 1 cut(s) 1008
AcsI RAATTY 3 cut(s) 795, 836, 1158
AdeI CACNNNGTG 1 cut(s) 724
AfaI GTAC 4 cut(s) 185, 689, 1367, 1550
AfiI CCNNNNNNNGG 7 cut(s) 172, 244, 482, 502, 610, 724, 1456
AgsI TTSAA 3 cut(s) 936, 1556, 1633
AjiI CACGTC 1 cut(s) 881
AjnI CCWGG 3 cut(s) 455, 608, 1717
AjuI GAANNNNNNNTTGG 6 cut(s) 191, 223, 626, 658, 899, 931
AluBI AGCT 8 cut(s) 422, 589, 680, 766, 915, 1210, 1297, 1408
AluI AGCT 8 cut(s) 422, 589, 680, 766, 915, 1210, 1297, 1408
Alw21I GWGCWC 4 cut(s) 579, 591, 846, 1299
Alw26I GTCTC 1 cut(s) 1517
AlwI GGATC 4 cut(s) 520, 1290, 1601, 1645
AlwNI CAGNNNCTG 1 cut(s) 1654
Ama87I CYCGRG 3 cut(s) 804, 827, 1625
AoxI GGCC 8 cut(s) 235, 246, 555, 594, 727, 1008, 1675, 1720
ApaI GGGCCC 1 cut(s) 598
ApeKI GCWGC 3 cut(s) 480, 1207, 1440
ApoI RAATTY 3 cut(s) 795, 836, 1158
Asp700I GAANNNNTTC 1 cut(s) 1356
AspS9I GGNCC 9 cut(s) 175, 246, 459, 556, 594, 595, 727, 1676, 1721
AsuC2I CCSGG 3 cut(s) 593, 848, 1349
AsuHPI GGTGA 5 cut(s) 202, 346, 1249, 1282, 1342
AsuII TTCGAA 1 cut(s) 792
AvaI CYCGRG 3 cut(s) 804, 827, 1625
AvaII GGWCC 2 cut(s) 175, 459
AxyI CCTNAGG 1 cut(s) 1338
BaeGI GKGCMC 2 cut(s) 598, 1349
BanI GGYRCC 1 cut(s) 1344
BanII GRGCYC 4 cut(s) 591, 598, 1231, 1299
BauI CACGAG 2 cut(s) 231, 1411
BbsI GAAGAC 3 cut(s) 607, 1166, 1349
Bbv12I GWGCWC 4 cut(s) 579, 591, 846, 1299
BbvI GCAGC 3 cut(s) 492, 1219, 1427
BccI CCATC 4 cut(s) 386, 826, 1148, 1425
BceAI ACGGC 3 cut(s) 706, 1023, 1204
BciT130I CCWGG 3 cut(s) 457, 610, 1719
BciVI GTATCC 1 cut(s) 1515
BcnI CCSGG 3 cut(s) 593, 848, 1349
BcoDI GTCTC 1 cut(s) 1517
BfaI CTAG 3 cut(s) 419, 873, 1725
BfuAI ACCTGC 1 cut(s) 163
BfuI GTATCC 1 cut(s) 1515
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 8 cut(s) 271, 481, 741, 756, 759, 1205, 1208, 1441
BlsI GCNGC 8 cut(s) 272, 482, 742, 757, 760, 1206, 1209, 1442
Bme1390I CCNGG 6 cut(s) 457, 593, 610, 848, 1349, 1719
Bme18I GGWCC 2 cut(s) 175, 459
BmeT110I CYCGRG 3 cut(s) 804, 827, 1625
BmgBI CACGTC 1 cut(s) 881
BmgT120I GGNCC 9 cut(s) 175, 246, 459, 556, 594, 595, 727, 1676, 1721
BmiI GGNNCC 3 cut(s) 461, 596, 1346
BmrFI CCNGG 6 cut(s) 457, 593, 610, 848, 1349, 1719
BmrI ACTGGG 2 cut(s) 253, 1673
BmsI GCATC 2 cut(s) 1048, 1723
BmuI ACTGGG 2 cut(s) 253, 1673
BoxI GACNNNNGTC 1 cut(s) 658
BpiI GAAGAC 3 cut(s) 607, 1166, 1349
BpmI CTGGAG 1 cut(s) 1548
Bpu14I TTCGAA 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 1283
BpuMI CCSGG 3 cut(s) 593, 848, 1349
BsaAI YACGTR 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 501, 609, 1011, 1450
BsaWI WCCGGW 1 cut(s) 705
Bsc4I CCNNNNNNNGG 7 cut(s) 172, 244, 482, 502, 610, 724, 1456
Bse118I RCCGGY 2 cut(s) 443, 1708
Bse1I ACTGG 4 cut(s) 248, 1431, 1531, 1679
Bse21I CCTNAGG 1 cut(s) 1338
Bse3DI GCAATG 1 cut(s) 1214
BseBI CCWGG 3 cut(s) 457, 610, 1719
BseDI CCNNGG 4 cut(s) 501, 609, 1011, 1450
BseGI GGATG 3 cut(s) 997, 1159, 1506
BseLI CCNNNNNNNGG 7 cut(s) 172, 244, 482, 502, 610, 724, 1456
BseMI GCAATG 1 cut(s) 1214
BseMII CTCAG 2 cut(s) 478, 1352
BseNI ACTGG 4 cut(s) 248, 1431, 1531, 1679
BseRI GAGGAG 7 cut(s) 41, 271, 281, 290, 334, 395, 1150
BseSI GKGCMC 2 cut(s) 598, 1349
BseX3I CGGCCG 1 cut(s) 1008
BseXI GCAGC 3 cut(s) 492, 1219, 1427
BseYI CCCAGC 2 cut(s) 476, 766
Bsh1285I CGRYCG 1 cut(s) 1011
BshFI GGCC 8 cut(s) 237, 248, 557, 596, 729, 1010, 1677, 1722
BshNI GGYRCC 1 cut(s) 1344
BsiEI CGRYCG 1 cut(s) 1011
BsiHKAI GWGCWC 4 cut(s) 579, 591, 846, 1299
BsiHKCI CYCGRG 3 cut(s) 804, 827, 1625
BsiSI CCGG 9 cut(s) 444, 546, 592, 706, 800, 848, 1250, 1349, 1709
BslFI GGGAC 2 cut(s) 445, 1257
BslI CCNNNNNNNGG 7 cut(s) 172, 244, 482, 502, 610, 724, 1456
BsmAI GTCTC 1 cut(s) 1517
BsmFI GGGAC 2 cut(s) 445, 1257
BsnI GGCC 8 cut(s) 237, 248, 557, 596, 729, 1010, 1677, 1722
BsoBI CYCGRG 3 cut(s) 804, 827, 1625
Bsp119I TTCGAA 1 cut(s) 792
Bsp120I GGGCCC 1 cut(s) 594
Bsp1286I GDGCHC 7 cut(s) 579, 591, 598, 846, 1231, 1299, 1349
Bsp143I GATC 4 cut(s) 525, 1282, 1606, 1637
BspANI GGCC 8 cut(s) 237, 248, 557, 596, 729, 1010, 1677, 1722
BspCNI CTCAG 2 cut(s) 477, 1351
BspLI GGNNCC 3 cut(s) 461, 596, 1346
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 4 cut(s) 520, 1290, 1601, 1645
BspT104I TTCGAA 1 cut(s) 792
BspT107I GGYRCC 1 cut(s) 1344
BsrBI CCGCTC 1 cut(s) 697
BsrDI GCAATG 1 cut(s) 1214
BsrFI RCCGGY 2 cut(s) 443, 1708
BsrI ACTGG 4 cut(s) 248, 1431, 1531, 1679
BssAI RCCGGY 2 cut(s) 443, 1708
BssECI CCNNGG 4 cut(s) 501, 609, 1011, 1450
BssMI GATC 4 cut(s) 525, 1282, 1606, 1637
BssSI CACGAG 2 cut(s) 231, 1411
Bst2BI CACGAG 2 cut(s) 231, 1411
Bst2UI CCWGG 3 cut(s) 457, 610, 1719
Bst4CI ACNGT 2 cut(s) 1111, 1669
Bst6I CTCTTC 3 cut(s) 103, 109, 356
BstAPI GCANNNNNTGC 1 cut(s) 1621
BstBAI YACGTR 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 792
BstC8I GCNNGC 4 cut(s) 579, 695, 699, 1212
BstDEI CTNAG 3 cut(s) 464, 968, 1338
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 997, 1159, 1506
BstKTI GATC 4 cut(s) 528, 1285, 1609, 1640
BstMAI GTCTC 1 cut(s) 1517
BstMBI GATC 4 cut(s) 525, 1282, 1606, 1637
BstMCI CGRYCG 1 cut(s) 1011
BstNI CCWGG 3 cut(s) 457, 610, 1719
BstNSI RCATGY 1 cut(s) 1565
BstPAI GACNNNNGTC 1 cut(s) 658
BstSCI CCNGG 6 cut(s) 455, 591, 608, 846, 1347, 1717
BstSLI GKGCMC 2 cut(s) 598, 1349
BstV1I GCAGC 3 cut(s) 492, 1219, 1427
BstV2I GAAGAC 3 cut(s) 607, 1166, 1349
BstZI CGGCCG 1 cut(s) 1008
Bsu36I CCTNAGG 1 cut(s) 1338
BsuI GTATCC 1 cut(s) 1515
BsuRI GGCC 8 cut(s) 237, 248, 557, 596, 729, 1010, 1677, 1722
BtrI CACGTC 1 cut(s) 881
BtsCI GGATG 3 cut(s) 997, 1159, 1506
BtsIMutI CAGTG 2 cut(s) 1674, 1686
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 4 cut(s) 579, 695, 699, 1212
CaiI CAGNNNCTG 1 cut(s) 1654
Cfr10I RCCGGY 2 cut(s) 443, 1708
Cfr13I GGNCC 9 cut(s) 175, 246, 459, 556, 594, 595, 727, 1676, 1721
CsiI ACCWGGT 1 cut(s) 455
Csp6I GTAC 4 cut(s) 184, 688, 1366, 1549
CviAII CATG 6 cut(s) 88, 731, 1436, 1456, 1562, 1616
CviQI GTAC 4 cut(s) 184, 688, 1366, 1549
DdeI CTNAG 3 cut(s) 464, 968, 1338
DpnI GATC 4 cut(s) 527, 1284, 1608, 1639
DpnII GATC 4 cut(s) 525, 1282, 1606, 1637
DraIII CACNNNGTG 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 1008
EagI CGGCCG 1 cut(s) 1008
Eam1104I CTCTTC 3 cut(s) 103, 109, 356
EarI CTCTTC 3 cut(s) 103, 109, 356
EciI GGCGGA 2 cut(s) 257, 776
Ecl136II GAGCTC 2 cut(s) 589, 1297
EclXI CGGCCG 1 cut(s) 1008
Eco24I GRGCYC 4 cut(s) 591, 598, 1231, 1299
Eco47I GGWCC 2 cut(s) 175, 459
Eco52I CGGCCG 1 cut(s) 1008
Eco53kI GAGCTC 2 cut(s) 589, 1297
Eco81I CCTNAGG 1 cut(s) 1338
Eco88I CYCGRG 3 cut(s) 804, 827, 1625
EcoICRI GAGCTC 2 cut(s) 589, 1297
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 175
EcoRI GAATTC 2 cut(s) 836, 1158
EcoRII CCWGG 3 cut(s) 455, 608, 1717
EcoT38I GRGCYC 4 cut(s) 591, 598, 1231, 1299
FaeI CATG 6 cut(s) 91, 734, 1439, 1459, 1565, 1619
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 899, 931
FaqI GGGAC 2 cut(s) 445, 1257
FatI CATG 6 cut(s) 87, 730, 1435, 1455, 1561, 1615
FauI CCCGC 4 cut(s) 221, 528, 666, 690
Fnu4HI GCNGC 8 cut(s) 271, 481, 741, 756, 759, 1205, 1208, 1441
FokI GGATG 3 cut(s) 1004, 1166, 1513
FriOI GRGCYC 4 cut(s) 591, 598, 1231, 1299
Fsp4HI GCNGC 8 cut(s) 271, 481, 741, 756, 759, 1205, 1208, 1441
FspBI CTAG 3 cut(s) 419, 873, 1725
GluI GCNGC 8 cut(s) 271, 481, 741, 756, 759, 1205, 1208, 1441
GsaI CCCAGC 2 cut(s) 480, 770
GsuI CTGGAG 1 cut(s) 1548
HaeIII GGCC 8 cut(s) 237, 248, 557, 596, 729, 1010, 1677, 1722
HapII CCGG 9 cut(s) 444, 546, 592, 706, 800, 848, 1250, 1349, 1709
Hin1II CATG 6 cut(s) 91, 734, 1439, 1459, 1565, 1619
HindIII AAGCTT 1 cut(s) 678
HinfI GANTC 4 cut(s) 413, 468, 542, 1651
HpaII CCGG 9 cut(s) 444, 546, 592, 706, 800, 848, 1250, 1349, 1709
HphI GGTGA 5 cut(s) 202, 346, 1249, 1282, 1342
Hpy166II GTNNAC 1 cut(s) 878
Hpy188I TCNGA 5 cut(s) 467, 541, 653, 928, 1068
Hpy188III TCNNGA 5 cut(s) 827, 1182, 1300, 1421, 1648
Hpy8I GTNNAC 1 cut(s) 878
Hpy99I CGWCG 2 cut(s) 323, 664
HpyAV CCTTC 9 cut(s) 40, 103, 120, 126, 808, 943, 1188, 1234, 1346
HpyCH4III ACNGT 2 cut(s) 1111, 1669
HpyCH4IV ACGT 3 cut(s) 182, 880, 1042
HpyCH4V TGCA 6 cut(s) 1168, 1375, 1483, 1565, 1580, 1615
HpyF3I CTNAG 3 cut(s) 464, 968, 1338
HpySE526I ACGT 3 cut(s) 182, 880, 1042
Hsp92II CATG 6 cut(s) 91, 734, 1439, 1459, 1565, 1619
Kzo9I GATC 4 cut(s) 525, 1282, 1606, 1637
LmnI GCTCC 4 cut(s) 571, 586, 594, 763
Lsp1109I GCAGC 3 cut(s) 492, 1219, 1427
LweI GCATC 2 cut(s) 1048, 1723
MabI ACCWGGT 1 cut(s) 455
MaeI CTAG 3 cut(s) 419, 873, 1725
MaeII ACGT 3 cut(s) 182, 880, 1042
MaeIII GTNAC 1 cut(s) 713
MalI GATC 4 cut(s) 527, 1284, 1608, 1639
MbiI CCGCTC 1 cut(s) 697
MboI GATC 4 cut(s) 525, 1282, 1606, 1637
MboII GAAGA 8 cut(s) 90, 96, 111, 343, 607, 1033, 1166, 1349
MfeI CAATTG 1 cut(s) 1566
MhlI GDGCHC 7 cut(s) 579, 591, 598, 846, 1231, 1299, 1349
MluCI AATT 7 cut(s) 795, 836, 1158, 1425, 1566, 1587, 1619
MlyI GAGTC 3 cut(s) 407, 477, 1660
MmeI TCCRAC 3 cut(s) 153, 631, 921
MroXI GAANNNNTTC 1 cut(s) 1356
MseI TTAA 1 cut(s) 1323
MslI CAYNNNNRTG 2 cut(s) 86, 1290
MspA1I CMGCKG 2 cut(s) 483, 1210
MspI CCGG 9 cut(s) 444, 546, 592, 706, 800, 848, 1250, 1349, 1709
MspR9I CCNGG 6 cut(s) 457, 593, 610, 848, 1349, 1719
MunI CAATTG 1 cut(s) 1566
MvaI CCWGG 3 cut(s) 457, 610, 1719
NciI CCSGG 3 cut(s) 593, 848, 1349
NdeII GATC 4 cut(s) 525, 1282, 1606, 1637
NlaIII CATG 6 cut(s) 91, 734, 1439, 1459, 1565, 1619
NlaIV GGNNCC 3 cut(s) 461, 596, 1346
NmeAIII GCCGAG 2 cut(s) 1036, 1431
NspI RCATGY 1 cut(s) 1565
NspV TTCGAA 1 cut(s) 792
PcsI WCGNNNNNNNCGW 1 cut(s) 1058
PdmI GAANNNNTTC 1 cut(s) 1356
PfeI GAWTC 1 cut(s) 542
PflMI CCANNNNNTGG 1 cut(s) 724
PkrI GCNGC 8 cut(s) 272, 482, 742, 757, 760, 1206, 1209, 1442
PleI GAGTC 3 cut(s) 407, 476, 1659
PpsI GAGTC 3 cut(s) 407, 476, 1659
Ppu21I YACGTR 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 175
PshAI GACNNNNGTC 1 cut(s) 658
Psp124BI GAGCTC 2 cut(s) 591, 1299
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 3 cut(s) 455, 608, 1717
PspFI CCCAGC 2 cut(s) 476, 766
PspGI CCWGG 3 cut(s) 455, 608, 1717
PspN4I GGNNCC 3 cut(s) 461, 596, 1346
PspOMI GGGCCC 1 cut(s) 594
PspPI GGNCC 9 cut(s) 175, 246, 459, 556, 594, 595, 727, 1676, 1721
PspPPI RGGWCCY 1 cut(s) 175
PsrI GAACNNNNNNTAC 2 cut(s) 1075, 1107
PstNI CAGNNNCTG 1 cut(s) 1654
PvuII CAGCTG 1 cut(s) 1210
RsaI GTAC 4 cut(s) 185, 689, 1367, 1550
RsaNI GTAC 4 cut(s) 184, 688, 1366, 1549
RseI CAYNNNNRTG 2 cut(s) 86, 1290
SacI GAGCTC 2 cut(s) 591, 1299
SaqAI TTAA 1 cut(s) 1323
SatI GCNGC 8 cut(s) 271, 481, 741, 756, 759, 1205, 1208, 1441
Sau3AI GATC 4 cut(s) 525, 1282, 1606, 1637
Sau96I GGNCC 9 cut(s) 175, 246, 459, 556, 594, 595, 727, 1676, 1721
SchI GAGTC 3 cut(s) 407, 477, 1660
ScrFI CCNGG 6 cut(s) 457, 593, 610, 848, 1349, 1719
SduI GDGCHC 7 cut(s) 579, 591, 598, 846, 1231, 1299, 1349
SexAI ACCWGGT 1 cut(s) 455
SfaNI GCATC 2 cut(s) 1048, 1723
SfuI TTCGAA 1 cut(s) 792
SinI GGWCC 2 cut(s) 175, 459
SmiMI CAYNNNNRTG 2 cut(s) 86, 1290
SmlI CTYRAG 1 cut(s) 1298
SmoI CTYRAG 1 cut(s) 1298
Sse9I AATT 7 cut(s) 795, 836, 1158, 1425, 1566, 1587, 1619
SspMI CTAG 3 cut(s) 419, 873, 1725
SstI GAGCTC 2 cut(s) 591, 1299
StyD4I CCNGG 6 cut(s) 455, 591, 608, 846, 1347, 1717
TaaI ACNGT 2 cut(s) 1111, 1669
TaiI ACGT 3 cut(s) 185, 883, 1045
TaqI TCGA 7 cut(s) 156, 240, 662, 792, 840, 1510, 1640
TaqII GACCGA 1 cut(s) 1526
TasI AATT 7 cut(s) 795, 836, 1158, 1425, 1566, 1587, 1619
TauI GCSGC 5 cut(s) 273, 743, 758, 761, 1207
TfiI GAWTC 1 cut(s) 542
Tru1I TTAA 1 cut(s) 1323
Tru9I TTAA 1 cut(s) 1323
TscAI CASTG 2 cut(s) 1674, 1686
TseI GCWGC 3 cut(s) 480, 1207, 1440
TspDTI ATGAA 1 cut(s) 1632
TspRI CASTG 2 cut(s) 1674, 1686
Van91I CCANNNNNTGG 1 cut(s) 724
VpaK11BI GGWCC 2 cut(s) 175, 459
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 3 cut(s) 795, 836, 1158
XceI RCATGY 1 cut(s) 1565
XmnI GAANNNNTTC 1 cut(s) 1356
XspI CTAG 3 cut(s) 419, 873, 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.