Rw2G013620

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0021660
Physical Location & Seq
Forward (+)
14745449 .. 14747822
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013620.1

Sequence Viewer

Length: 570 bp
ATGCCTGAGATTGAGGGTTCTGATGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACGACCTTCACCAAAGGAGGAAGTTGGGGAAGTGAGCGAGACATTGGCTTCAATAAGTAACCTAAAGGAGGTAGTCAAGCAATATGAAGGGCCTAGTGTCATACAAAATCAACATGAGATTATAGGTTCCTGCAAAGTACCTGAACCTTCATCGCCTACAGTGATGCCTGAGATTGAGGATTCTGTTGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACTACCTTCACCAAAGGTTCTAATTACATCTGAACCGGAGTCCCAGCCAATGAAAGGTCAATGGCAAAAGGGAAATCTTATTGGTCGTGGCACGTTTGGGAATGTTTATGTTGCTTCCAATGGGGACATCAAAGGGGATAATTTGCTCGTTGAGTCATCTGGTGTTGTCAAGATAGCTGACTTTGGAATTGTAAAACATAGCCAATTTGGTGGCCACAAACCCAATCAGCAACGAAACCCCAATCACAAGCCTCAAAATCGCAATGGGGAAGCAAACCCCGATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

189

Amino Acids

20.3

Weight (kDa)

5.53

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021660)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002892.1_g000072 Rmu_sc0020206.1_g000002 Rmu_sc0020207.1_g000002
rosa_samantha Rh2DG261900
rosa_wichuraiana Rw2G013620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 493
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 2 cut(s) 127, 335
AgsI TTSAA 2 cut(s) 111, 248
AjnI CCWGG 2 cut(s) 49, 271
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 92
AoxI GGCC 2 cut(s) 149, 493
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 60, 282
BalI TGGCCA 1 cut(s) 495
BciT130I CCWGG 2 cut(s) 51, 273
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 51, 273
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 2 cut(s) 51, 273
BmsI GCATC 3 cut(s) 40, 213, 262
BpmI CTGGAG 2 cut(s) 72, 294
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 335
Bse1I ACTGG 2 cut(s) 41, 263
Bse3DI GCAATG 1 cut(s) 550
BseBI CCWGG 2 cut(s) 51, 273
BseGI GGATG 2 cut(s) 31, 253
BseLI CCNNNNNNNGG 2 cut(s) 127, 335
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 41, 263
BseYI CCCAGC 1 cut(s) 324
BshFI GGCC 2 cut(s) 151, 495
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 307, 419
BslI CCNNNNNNNGG 2 cut(s) 127, 335
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 307, 419
BsnI GGCC 2 cut(s) 151, 495
Bsp143I GATC 1 cut(s) 562
BspANI GGCC 2 cut(s) 151, 495
BspCNI CTCAG 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 550
BsrI ACTGG 2 cut(s) 41, 263
BssMI GATC 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 51, 273
Bst4CI ACNGT 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 6, 228
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 31, 253
BstKTI GATC 1 cut(s) 565
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 1 cut(s) 562
BstNI CCWGG 2 cut(s) 51, 273
BstSCI CCNGG 2 cut(s) 49, 271
BstSFI CTRYAG 1 cut(s) 216
BstXI CCANNNNNNTGG 1 cut(s) 491
BsuRI GGCC 2 cut(s) 151, 495
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 2 cut(s) 31, 253
BtsIMutI CAGTG 3 cut(s) 48, 225, 270
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 197
CviAII CATG 1 cut(s) 173
CviJI RGCY 7 cut(s) 107, 151, 328, 458, 483, 495, 532
CviKI_1 RGCY 7 cut(s) 107, 151, 328, 458, 483, 495, 532
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 2 cut(s) 6, 228
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 49, 271
FaeI CATG 1 cut(s) 176
FaiI YATR 6 cut(s) 144, 161, 174, 182, 390, 480
FaqI GGGAC 2 cut(s) 307, 419
FatI CATG 1 cut(s) 172
FokI GGATG 2 cut(s) 18, 240
FspBI CTAG 1 cut(s) 153
GsaI CCCAGC 1 cut(s) 328
GsuI CTGGAG 2 cut(s) 72, 294
HaeIII GGCC 2 cut(s) 151, 495
HapII CCGG 1 cut(s) 317
Hin1II CATG 1 cut(s) 176
HinfI GANTC 3 cut(s) 239, 320, 434
HpaII CCGG 1 cut(s) 317
HphI GGTGA 2 cut(s) 60, 282
Hpy188I TCNGA 2 cut(s) 22, 313
Hpy188III TCNNGA 1 cut(s) 451
HpyAV CCTTC 4 cut(s) 75, 140, 216, 297
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 31, 192, 253
HpyF3I CTNAG 2 cut(s) 6, 228
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 1 cut(s) 176
Kzo9I GATC 1 cut(s) 562
LmnI GCTCC 2 cut(s) 53, 275
LweI GCATC 3 cut(s) 40, 213, 262
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 116
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 4 cut(s) 303, 421, 468, 485
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 2 cut(s) 329, 443
MnlI CCTC 7 cut(s) 7, 57, 70, 121, 229, 279, 543
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MseI TTAA 1 cut(s) 568
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 2 cut(s) 51, 273
MvaI CCWGG 2 cut(s) 51, 273
NdeII GATC 1 cut(s) 562
NlaIII CATG 1 cut(s) 176
NlaIV GGNNCC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 239
PfoI TCCNGGA 2 cut(s) 49, 271
PleI GAGTC 2 cut(s) 328, 442
PpsI GAGTC 2 cut(s) 328, 442
Psp6I CCWGG 2 cut(s) 49, 271
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 2 cut(s) 49, 271
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 1 cut(s) 149
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SaqAI TTAA 1 cut(s) 568
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 2 cut(s) 329, 443
ScrFI CCNGG 2 cut(s) 51, 273
SfaNI GCATC 3 cut(s) 40, 213, 262
SfcI CTRYAG 1 cut(s) 216
Sse9I AATT 4 cut(s) 303, 421, 468, 485
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 2 cut(s) 49, 271
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 1 cut(s) 377
TasI AATT 4 cut(s) 303, 421, 468, 485
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 568
Tru9I TTAA 1 cut(s) 568
TscAI CASTG 3 cut(s) 48, 225, 270
TspDTI ATGAA 4 cut(s) 39, 159, 198, 347
TspRI CASTG 3 cut(s) 48, 225, 270
XagI CCTNNNNNAGG 1 cut(s) 125
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.