Rw4G030130

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017166
Physical Location & Seq
Reverse (-)
56833609 .. 56834873
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030130.1

Sequence Viewer

Length: 1230 bp
ATGGTCTCACTCTCCCAACTCCCATTCTTCTTCTTCTTCTTCACAACCCTCTGCAACTCCCAACACTACCTCCAACTCCCGCTTCTCCACACAGACCCCTTCCCCACACCCTCCCAAGCCCTCTCTCTCGACACCCACCGCCTCTCTCTCCTCCACAACCACAACCGCCGCCGCTCCGCCATCTCCCCCGTCGTCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCCACCTCCGCCTCGGCTCTCCCCCCCAGAGCCTCCTCCTCGTCGCCGACACCGGCAGCGATCTCGTCTGGCTCCGCTGCTCCGCCTGCAAAAGCTGCTCTAATCGGAGCCCCGGCTCCGCTTTCCTCGCCCGCCACTCGACCACATTCTCACCCTACCACTGCTACGACTCCACCTGCGACCTCGTCCCCCGACCCGACCCGAGTCCGTGTAACCATACCCGGCTCCACAGCCCCTGTCGCTACGAGTATTCCTACTCCGACGGATCCAGAACCGCCGGCTTCTTCTCCAGAGAAACCACCACTCTAAACACCAGCTCCGGGACACCGGCGAAGCTCTCCGACTTGGCGTTCGGGTGCGGGTTTGATGTTTCGGGTCCGAGCTTAACCGGGGCGAGCCTCGGCGGGGCCCACGGCGTCATGGGCCTGGGAAGAGGCCCAATCTCGTTTGTCTCGCAGCTGGGTCGCCGATTCGGGAACAAATTCTCCTACTGTCTACTGGACTACACCCGATCGCCGCCGCCGACTAGTTACCTCAGGATCGGAGCCTCCAAATCCGACGTCGTTTCGAAACTGAGCTTCACACGCTTGCTGCTCAACCCTCTCTCCCCTACATTTTACTACATTGGTATCAAGTCCGTCTCCGTCAACGGCGTTAAATTACCGGTTCGCCCCTCCGTTTGGTCCCTCGACGAGCTCGGTAACGGCGGCACGGTGATCGACTCGGGGACGACGTTAACTTTCTTGCCCGAGCCGGCTTACCGTCTAATCCTGGCGGCCCGAGCCGACTCCGGACTCGCGGGTGGCTCGGTTTTCGCGCCGCCGCCGGGGAATTATTTCGTTGAGACAATGGATCGGGTCGAGTGCTTGGCGATCCAACCAGTTGATTCCGGGTCGGGCTTTTCGGTGATAGGGAATCTTATGCAACAAGGATTCTTGTTTGAGTTTGACAAGGACCGCTCGCGGCTCGGTTTTTCACGTCATGGCTGCGCGCTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04138 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04138 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

409

Amino Acids

44.25

Weight (kDa)

8.91

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 74 - 330 9.5e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 75 - 258 7.3e-47 Xylanase inhibitor N-terminal
TAXi_C PF14541 283 - 336 2.9e-12 Xylanase inhibitor C-terminal
TAXi_C PF14541 342 - 402 1.6e-08 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25700 AT3G25700
fragaria_vesca FvH4_4g28140
malus_domestica MD16G1048900.v1.1
prunus_persica Prupe.1G304800_v2.0.a1
pyrus_communis pycom16g04270
rosa_chinensis RchiOBHm_Chr4g0435911
rosa_laevigata RLG00000006550
rosa_roxburghii Rroxscaffold_5G00377090
rosa_rugosa Rorug04G0290700
rosa_samantha Rh4BG353800 Rh4DG347600
rosa_wichuraiana Rw4G030130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 413
AatII GACGTC 1 cut(s) 792
Acc36I ACCTGC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 200
AccBSI CCGCTC 2 cut(s) 174, 1188
AccI GTMKAC 1 cut(s) 724
AccII CGCG 4 cut(s) 1028, 1046, 1192, 1220
AccIII TCCGGA 1 cut(s) 1019
AclWI GGATC 5 cut(s) 489, 502, 776, 1089, 1096
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 1 cut(s) 710
AcuI CTGAAG 1 cut(s) 1208
AcyI GRCGYC 3 cut(s) 201, 645, 789
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 4 cut(s) 549, 634, 909, 1055
AgeI ACCGGT 1 cut(s) 892
AhlI ACTAGT 1 cut(s) 755
AjiI CACGTC 1 cut(s) 1208
AjnI CCWGG 2 cut(s) 654, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 7 cut(s) 324, 546, 565, 612, 688, 807, 925
AluI AGCT 7 cut(s) 324, 546, 565, 612, 688, 807, 925
Alw21I GWGCWC 1 cut(s) 927
Alw26I GTCTC 5 cut(s) 10, 199, 685, 874, 1067
AlwI GGATC 5 cut(s) 489, 502, 776, 1089, 1096
AlwNI CAGNNNCTG 1 cut(s) 465
Ama87I CYCGRG 4 cut(s) 430, 952, 977, 1008
Aor13HI TCCGGA 1 cut(s) 1019
AoxI GGCC 5 cut(s) 217, 636, 652, 664, 1005
ApaI GGGCCC 1 cut(s) 640
ApeKI GCWGC 6 cut(s) 285, 306, 324, 685, 820, 1215
ApoI RAATTY 1 cut(s) 710
ArsI GACNNNNNNTTYG 4 cut(s) 563, 595, 774, 806
AsiGI ACCGGT 1 cut(s) 892
Asp700I GAANNNNTTC 2 cut(s) 710, 1064
AspLEI GCGC 4 cut(s) 203, 1048, 1220, 1222
AspS9I GGNCC 8 cut(s) 605, 636, 637, 652, 665, 912, 1006, 1183
AsuC2I CCSGG 6 cut(s) 342, 451, 550, 619, 1056, 1120
AsuHPI GGTGA 3 cut(s) 372, 955, 1147
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 4 cut(s) 430, 952, 977, 1008
AvaII GGWCC 3 cut(s) 605, 912, 1183
AxyI CCTNAGG 1 cut(s) 764
BaeGI GKGCMC 1 cut(s) 640
BaeI ACNNNNGTAYC 2 cut(s) 841, 874
BamHI GGATCC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 3 cut(s) 341, 640, 927
Bbv12I GWGCWC 1 cut(s) 927
BbvI GCAGC 6 cut(s) 293, 297, 311, 697, 807, 1202
BccI CCATC 1 cut(s) 188
BceAI ACGGC 3 cut(s) 658, 895, 949
BcgI CGANNNNNNTGC 6 cut(s) 274, 308, 674, 708, 928, 962
BciT130I CCWGG 2 cut(s) 656, 1001
BcnI CCSGG 6 cut(s) 342, 451, 550, 619, 1056, 1120
BcoDI GTCTC 5 cut(s) 10, 199, 685, 874, 1067
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 1 cut(s) 756
BfoI RGCGCY 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 413
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 8 cut(s) 342, 451, 550, 619, 656, 1001, 1056, 1120
Bme18I GGWCC 3 cut(s) 605, 912, 1183
BmeT110I CYCGRG 4 cut(s) 430, 952, 977, 1008
BmgBI CACGTC 1 cut(s) 1208
BmgT120I GGNCC 8 cut(s) 605, 636, 637, 652, 665, 912, 1006, 1183
BmrFI CCNGG 8 cut(s) 342, 451, 550, 619, 656, 1001, 1056, 1120
BoxI GACNNNNGTC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 502
Bpu14I TTCGAA 1 cut(s) 797
BpuMI CCSGG 6 cut(s) 342, 451, 550, 619, 1056, 1120
BsaHI GRCGYC 3 cut(s) 201, 645, 789
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 7 cut(s) 241, 340, 618, 628, 640, 655, 1055
BsaWI WCCGGW 2 cut(s) 892, 1019
BsaXI ACNNNNNCTCC 8 cut(s) 54, 84, 530, 560, 698, 728, 818, 848
Bsc4I CCNNNNNNNGG 4 cut(s) 549, 634, 909, 1055
Bse118I RCCGGY 6 cut(s) 209, 281, 506, 556, 892, 982
Bse1I ACTGG 3 cut(s) 220, 732, 1109
Bse21I CCTNAGG 1 cut(s) 764
BseAI TCCGGA 1 cut(s) 1019
BseBI CCWGG 2 cut(s) 656, 1001
BseDI CCNNGG 7 cut(s) 241, 340, 618, 628, 640, 655, 1055
BseLI CCNNNNNNNGG 4 cut(s) 549, 634, 909, 1055
BseMII CTCAG 2 cut(s) 778, 794
BseNI ACTGG 3 cut(s) 220, 732, 1109
BsePI GCGCGC 1 cut(s) 1218
BseRI GAGGAG 3 cut(s) 140, 254, 257
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 6 cut(s) 293, 297, 311, 697, 807, 1202
BseYI CCCAGC 1 cut(s) 688
Bsh1236I CGCG 4 cut(s) 1028, 1046, 1192, 1220
Bsh1285I CGRYCG 1 cut(s) 743
BshFI GGCC 5 cut(s) 219, 638, 654, 666, 1007
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 892
BsiEI CGRYCG 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 4 cut(s) 430, 952, 977, 1008
BslFI GGGAC 4 cut(s) 401, 565, 898, 970
BslI CCNNNNNNNGG 4 cut(s) 549, 634, 909, 1055
BsmAI GTCTC 5 cut(s) 10, 199, 685, 874, 1067
BsmBI CGTCTC 2 cut(s) 199, 874
BsmFI GGGAC 4 cut(s) 401, 565, 898, 970
BsnI GGCC 5 cut(s) 219, 638, 654, 666, 1007
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 4 cut(s) 430, 952, 977, 1008
Bsp119I TTCGAA 1 cut(s) 797
Bsp120I GGGCCC 1 cut(s) 636
Bsp1286I GDGCHC 3 cut(s) 341, 640, 927
Bsp13I TCCGGA 1 cut(s) 1019
Bsp143I GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
BspANI GGCC 5 cut(s) 219, 638, 654, 666, 1007
BspCNI CTCAG 2 cut(s) 777, 795
BspEI TCCGGA 1 cut(s) 1019
BspFNI CGCG 4 cut(s) 1028, 1046, 1192, 1220
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 5 cut(s) 489, 502, 776, 1089, 1096
BspT104I TTCGAA 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 10
BsrBI CCGCTC 2 cut(s) 174, 1188
BsrFI RCCGGY 6 cut(s) 209, 281, 506, 556, 892, 982
BsrI ACTGG 3 cut(s) 220, 732, 1109
BssAI RCCGGY 6 cut(s) 209, 281, 506, 556, 892, 982
BssECI CCNNGG 7 cut(s) 241, 340, 618, 628, 640, 655, 1055
BssHII GCGCGC 1 cut(s) 1218
BssMI GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
BssNI GRCGYC 3 cut(s) 201, 645, 789
Bst2UI CCWGG 2 cut(s) 656, 1001
Bst4CI ACNGT 3 cut(s) 722, 943, 992
Bst6I CTCTTC 1 cut(s) 655
BstACI GRCGYC 3 cut(s) 201, 645, 789
BstAPI GCANNNNNTGC 1 cut(s) 324
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 8 cut(s) 316, 361, 508, 625, 818, 984, 1190, 1220
BstDEI CTNAG 2 cut(s) 764, 803
BstDSI CCRYGG 1 cut(s) 640
BstFNI CGCG 4 cut(s) 1028, 1046, 1192, 1220
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 4 cut(s) 203, 1048, 1220, 1222
BstKTI GATC 7 cut(s) 292, 497, 743, 771, 948, 1084, 1104
BstMAI GTCTC 5 cut(s) 10, 199, 685, 874, 1067
BstMBI GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
BstMCI CGRYCG 1 cut(s) 743
BstMWI GCNNNNNNNGC 7 cut(s) 315, 324, 356, 468, 651, 813, 1010
BstNI CCWGG 2 cut(s) 656, 1001
BstPAI GACNNNNGTC 1 cut(s) 432
BstSCI CCNGG 8 cut(s) 340, 449, 548, 617, 654, 999, 1054, 1118
BstSLI GKGCMC 1 cut(s) 640
BstUI CGCG 4 cut(s) 1028, 1046, 1192, 1220
BstV1I GCAGC 6 cut(s) 293, 297, 311, 697, 807, 1202
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
Bsu36I CCTNAGG 1 cut(s) 764
BsuRI GGCC 5 cut(s) 219, 638, 654, 666, 1007
BtgI CCRYGG 1 cut(s) 640
BtrI CACGTC 1 cut(s) 1208
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 8 cut(s) 316, 361, 508, 625, 818, 984, 1190, 1220
CaiI CAGNNNCTG 1 cut(s) 465
CfoI GCGC 4 cut(s) 203, 1048, 1220, 1222
Cfr10I RCCGGY 6 cut(s) 209, 281, 506, 556, 892, 982
Cfr13I GGNCC 8 cut(s) 605, 636, 637, 652, 665, 912, 1006, 1183
CseI GACGC 1 cut(s) 634
Csp6I GTAC 1 cut(s) 223
CspAI ACCGGT 1 cut(s) 892
CviAII CATG 2 cut(s) 649, 1211
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 764, 803
DinI GGCGCC 1 cut(s) 202
DpnI GATC 7 cut(s) 291, 496, 742, 770, 947, 1083, 1103
DpnII GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 655
EarI CTCTTC 1 cut(s) 655
EciI GGCGGA 3 cut(s) 166, 227, 301
Ecl136II GAGCTC 1 cut(s) 925
Eco24I GRGCYC 3 cut(s) 341, 640, 927
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 3 cut(s) 605, 912, 1183
Eco53kI GAGCTC 1 cut(s) 925
Eco57I CTGAAG 1 cut(s) 1208
Eco81I CCTNAGG 1 cut(s) 764
Eco88I CYCGRG 4 cut(s) 430, 952, 977, 1008
EcoICRI GAGCTC 1 cut(s) 925
EcoO109I RGGNCCY 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 654, 999
EcoT38I GRGCYC 3 cut(s) 341, 640, 927
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
Esp3I CGTCTC 2 cut(s) 199, 874
FaeI CATG 2 cut(s) 652, 1214
FaiI YATR 4 cut(s) 447, 650, 1151, 1212
FaqI GGGAC 4 cut(s) 401, 565, 898, 970
FatI CATG 2 cut(s) 648, 1210
FauI CCCGC 5 cut(s) 87, 368, 581, 626, 1021
FblI GTMKAC 1 cut(s) 724
FriOI GRGCYC 3 cut(s) 341, 640, 927
FspBI CTAG 1 cut(s) 756
GlaI GCGC 4 cut(s) 202, 1047, 1219, 1221
GsaI CCCAGC 1 cut(s) 692
GsuI CTGGAG 1 cut(s) 502
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 5 cut(s) 219, 638, 654, 666, 1007
HgaI GACGC 1 cut(s) 634
HhaI GCGC 4 cut(s) 203, 1048, 1220, 1222
Hin1I GRCGYC 3 cut(s) 201, 645, 789
Hin1II CATG 2 cut(s) 652, 1214
Hin6I GCGC 4 cut(s) 201, 1046, 1218, 1220
HinP1I GCGC 4 cut(s) 201, 1046, 1218, 1220
HincII GTYRAC 2 cut(s) 877, 966
HindII GTYRAC 2 cut(s) 877, 966
HinfI GANTC 9 cut(s) 398, 433, 699, 950, 1016, 1023, 1115, 1144, 1161
HpaI GTTAAC 1 cut(s) 966
HphI GGTGA 3 cut(s) 372, 955, 1147
Hpy166II GTNNAC 4 cut(s) 232, 725, 877, 966
Hpy188I TCNGA 6 cut(s) 336, 490, 571, 609, 773, 787
Hpy188III TCNNGA 6 cut(s) 128, 498, 519, 703, 766, 1020
Hpy8I GTNNAC 4 cut(s) 232, 725, 877, 966
Hpy99I CGWCG 7 cut(s) 194, 275, 494, 791, 794, 923, 964
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 3 cut(s) 722, 943, 992
HpyCH4IV ACGT 3 cut(s) 789, 962, 1207
HpyCH4V TGCA 3 cut(s) 54, 318, 1153
HpyF10VI GCNNNNNNNGC 7 cut(s) 315, 324, 356, 468, 651, 813, 1010
HpyF3I CTNAG 2 cut(s) 764, 803
HpySE526I ACGT 3 cut(s) 789, 962, 1207
Hsp92I GRCGYC 3 cut(s) 201, 645, 789
Hsp92II CATG 2 cut(s) 652, 1214
HspAI GCGC 4 cut(s) 201, 1046, 1218, 1220
KasI GGCGCC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 1019
KroI GCCGGC 2 cut(s) 506, 982
KroNI GCCGGC 2 cut(s) 508, 984
KspAI GTTAAC 1 cut(s) 966
Kzo9I GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
LmnI GCTCC 9 cut(s) 179, 218, 306, 314, 336, 350, 459, 551, 773
Lsp1109I GCAGC 6 cut(s) 293, 297, 311, 697, 807, 1202
MaeI CTAG 1 cut(s) 756
MaeII ACGT 3 cut(s) 789, 962, 1207
MaeIII GTNAC 3 cut(s) 440, 758, 929
MalI GATC 7 cut(s) 291, 496, 742, 770, 947, 1083, 1103
MbiI CCGCTC 2 cut(s) 174, 1188
MboI GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
MboII GAAGA 7 cut(s) 19, 22, 25, 28, 31, 505, 672
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 3 cut(s) 341, 640, 927
MluCI AATT 3 cut(s) 710, 887, 1060
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 5 cut(s) 392, 442, 944, 1010, 1017
MmeI TCCRAC 5 cut(s) 97, 513, 594, 810, 1129
MroI TCCGGA 1 cut(s) 1019
MroNI GCCGGC 2 cut(s) 506, 982
MroXI GAANNNNTTC 2 cut(s) 710, 1064
MseI TTAA 3 cut(s) 614, 885, 965
MspA1I CMGCKG 2 cut(s) 306, 688
MspR9I CCNGG 8 cut(s) 342, 451, 550, 619, 656, 1001, 1056, 1120
MvaI CCWGG 2 cut(s) 656, 1001
MvnI CGCG 4 cut(s) 1028, 1046, 1192, 1220
MwoI GCNNNNNNNGC 7 cut(s) 315, 324, 356, 468, 651, 813, 1010
NaeI GCCGGC 2 cut(s) 508, 984
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 6 cut(s) 342, 451, 550, 619, 1056, 1120
NdeII GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
NgoMIV GCCGGC 2 cut(s) 506, 982
NlaIII CATG 2 cut(s) 652, 1214
NmeAIII GCCGAG 2 cut(s) 222, 609
NspV TTCGAA 1 cut(s) 797
PaqCI CACCTGC 1 cut(s) 413
PauI GCGCGC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 2 cut(s) 749, 959
PdiI GCCGGC 2 cut(s) 508, 984
PdmI GAANNNNTTC 2 cut(s) 710, 1064
PfeI GAWTC 4 cut(s) 699, 1115, 1144, 1161
PflFI GACNNNGTC 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 548
PinAI ACCGGT 1 cut(s) 892
Ple19I CGATCG 1 cut(s) 743
PleI GAGTC 5 cut(s) 392, 441, 944, 1010, 1017
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 5 cut(s) 392, 441, 944, 1010, 1017
PshAI GACNNNNGTC 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 927
Psp6I CCWGG 2 cut(s) 654, 999
PspFI CCCAGC 1 cut(s) 688
PspGI CCWGG 2 cut(s) 654, 999
PspOMI GGGCCC 1 cut(s) 636
PspPI GGNCC 8 cut(s) 605, 636, 637, 652, 665, 912, 1006, 1183
PstNI CAGNNNCTG 1 cut(s) 465
PsuI RGATCY 1 cut(s) 494
PsyI GACNNNGTC 1 cut(s) 413
PteI GCGCGC 1 cut(s) 1218
PvuI CGATCG 1 cut(s) 743
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacI GAGCTC 1 cut(s) 927
SaqAI TTAA 3 cut(s) 614, 885, 965
Sau3AI GATC 7 cut(s) 289, 494, 740, 768, 945, 1081, 1101
Sau96I GGNCC 8 cut(s) 605, 636, 637, 652, 665, 912, 1006, 1183
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 5 cut(s) 392, 442, 944, 1010, 1017
ScrFI CCNGG 8 cut(s) 342, 451, 550, 619, 656, 1001, 1056, 1120
SduI GDGCHC 3 cut(s) 341, 640, 927
SfoI GGCGCC 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 797
SgrAI CRCCGGYG 1 cut(s) 556
SinI GGWCC 3 cut(s) 605, 912, 1183
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 3 cut(s) 710, 887, 1060
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 1 cut(s) 756
SstI GAGCTC 1 cut(s) 927
StyD4I CCNGG 8 cut(s) 340, 449, 548, 617, 654, 999, 1054, 1118
TaaI ACNGT 3 cut(s) 722, 943, 992
TaiI ACGT 3 cut(s) 792, 965, 1210
TaqI TCGA 6 cut(s) 129, 368, 797, 918, 948, 1089
TasI AATT 3 cut(s) 710, 887, 1060
TatI WGTACW 1 cut(s) 222
TauI GCSGC 9 cut(s) 171, 174, 748, 751, 939, 1007, 1051, 1054, 1195
TfiI GAWTC 4 cut(s) 699, 1115, 1144, 1161
Tru1I TTAA 3 cut(s) 614, 885, 965
Tru9I TTAA 3 cut(s) 614, 885, 965
TscAI CASTG 1 cut(s) 395
TseI GCWGC 6 cut(s) 285, 306, 324, 685, 820, 1215
TspGWI ACGGA 5 cut(s) 426, 507, 856, 862, 895
TspRI CASTG 1 cut(s) 395
Tth111I GACNNNGTC 1 cut(s) 413
VpaK11BI GGWCC 3 cut(s) 605, 912, 1183
XapI RAATTY 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 724
XmnI GAANNNNTTC 2 cut(s) 710, 1064
XspI CTAG 1 cut(s) 756
ZraI GACGTC 1 cut(s) 790
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.